[Histonet] chicken EGFP aby
Does anyone have experience with detecting EGFP with some of the current aby's made in chicken? We have used aby's from both AVES labs and Millipore with negative results. In both cases we used the biotinylated secondary aby from AVES followed by a fluorescent streptavidin. Absolutely no staining. Using the same tissue, we were able to use a different aby made in rabbit and it worked great. However, because of double-labeling needs we'd really like to get the chicken to work. Perhaps someone has experience or insights? Thanks! Susan Travers Division of Oral Biology College of Dentistry The Ohio State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] chicken EGFP aby
I've had good luck using Abcam's chicken polyclonal: http://www.abcam.com/GFP-antibody-ab13970.html I use a directly conjugated secondary, and the staining is usually pretty bright. But then again, my GFP expression is high. Adam On Thu, Oct 7, 2010 at 7:44 AM, Susan Travers traver...@osu.edu wrote: Does anyone have experience with detecting EGFP with some of the current aby's made in chicken? We have used aby's from both AVES labs and Millipore with negative results. In both cases we used the biotinylated secondary aby from AVES followed by a fluorescent streptavidin. Absolutely no staining. Using the same tissue, we were able to use a different aby made in rabbit and it worked great. However, because of double-labeling needs we'd really like to get the chicken to work. Perhaps someone has experience or insights? Thanks! Susan Travers Division of Oral Biology College of Dentistry The Ohio State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Training
Does anyone know of any GLP training in El Paso, TX? Kendra Leigh Carter -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, October 06, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 83, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Helicobacter pylori (Laurie Colbert) 2. RE: AChE fiber staining protocoll? (szi...@bio.u-szeged.hu) 3. RE: Helicobacter pylori (Weems, Joyce) 4. PAS STAIN (Diana McCaig) 5. Histokinette 2000 (Biedermann, JoAnn) 6. RE: PAS STAIN (Rathborne, Toni) 7. Query Special Stainers (Joanne Clark) 8. Re: Helicobacter pylori (Brandi Higgins) 9. AW: [Histonet] PAS STAIN (Gudrun Lang) 10. Where to buy Michels RE: [Histonet] DIF Transport Media (gayle callis) 11. RE: Query Special Stainers (Mahoney,Janice A) 12. RE: PAS STAIN (gayle callis) 13. RE: Query Special Stainers (Marcia Funk) 14. PAS staining (Janet Keeping) -- Message: 1 Date: Wed, 6 Oct 2010 07:48:25 -0700 From: Laurie Colbert laurie.colb...@huntingtonhospital.com Subject: RE: [Histonet] Helicobacter pylori To: Kathy M. Gorham km...@grh.org, histonet@lists.utsouthwestern.edu Message-ID: 57be698966d5c54eae8612e8941d768309a5a...@exchange3.huntingtonhospital.com Content-Type: text/plain; charset=us-ascii We basically do a dif quik stain. I buy a kit called Three Step Stain from Cardinal. The total staining process takes about 1 minute. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, October 06, 2010 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter pylori Good Morning Histo land. I would like to know what others are using for a special stain for Helicobacter pylori not IHC. Do you use a kit? From where? Make up your own? Procedure. Thanks you. You have always been so helpful. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Wed, 06 Oct 2010 17:00:35 +0200 From: szi...@bio.u-szeged.hu Subject: RE: [Histonet] AChE fiber staining protocoll? To: Houston, Ronald ronald.hous...@nationwidechildrens.org Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 20101006170035.j2waztulso448...@webmail.u-szeged.hu Content-Type: text/plain; charset=ISO-8859-2; DelSp=Yes; format=flowed Idézet (Houston, Ronald ronald.hous...@nationwidechildrens.org): Thank you for the answer. We will try this protocol and i will reply the results. Csaba Szigeti I have had great success in fine fibers with the modification of Martucciello et al, Eur J Pediatr Surg 2001; 11: 300-304, but only in 15 micron intestine sections, so I do not know how it will transpose to brain studies and the thicker sections Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722
[Histonet] problems with staining IHC
Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] problems with staining IHC
Are you staining by hand or are you automated? Dana Settembre University Hospital Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Thursday, October 07, 2010 10:32 AM To: histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] problems with staining IHC
Hi Mala, I had this same problem. It was solved by adding Tween to the buffer. It made the reagents flow much better over the slides. Also before you stain, put the slides in a rinse of buffer/tween and agitate them for about 30 seconds. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Thursday, October 07, 2010 10:32 AM To: histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] problems with staining IHC
What kind of stainers are you using and is it happening on all your stainers? Or are you staining by hand? Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Thursday, October 07, 2010 9:32 AM To: histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problems with staining IHC
Mala, If your main problem is that the reagents aren't spreading across your slides, I agree that you should put Tween 20 (0.05% per volume) in all of your rinse buffers between steps, regardless of if you do your IHC stains manually or with an autostainer. If this doesn't solve the problem, post your method on histonet so that we know more about your procedure steps and can send additional advice. Jan Shivers UMN Vet Diag Lab - Original Message - From: sris...@mail.holyname.org To: histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Sent: Thursday, October 07, 2010 9:32 AM Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] problems with staining IHC
Hi Mala, We've used the Fisher superfrost plus slides for years and have never had that issue, it sounds more like you need to add some Tween to your buffer. That will help the solutions spread along your slide. Hope that helps. Thanks, Timothy Higgins, HT(ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Thursday, October 07, 2010 9:32 AM To: histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Equipment Purchase Questions
Hello Histo Community, We are currently looking to purchase several items. I'm hoping someone can shed light on whether or not we are headed in the right direction. Processor: Initially we were leaning towards the Leica ASP 300, now I'm wondering if we are about to purchase to much processor for the needs of our lab. We process maybe 50 blocks per month. We are unable to use a microwave processor. We process about 95% breast tissue. Someone mentioned the TP1020. Has anyone any comments on this processor. We are also looking to purchase a ph meter and a fume adsorber, any recommendations ? Your responses will be greatly appreciated as we are looking to purchase before the end of the month. Thanks for all your help and suggestions, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcru...@path.wustl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Equipment Purchase Questions
Karen, I would highly recommend Leica's Peloris processor. We use it for rapid tissue processing (2 hour processing time for cores and small specimens). It gives us the option of using one retort for rapid processing and the other for more conventional processing. We are also saving on reagents since we do not have to change out the entire processor weekly or twice per week. Peloris keeps track of the reagents and lets us know when one of them needs to be changed. We have been using our two units for a little over 9 months and we have yet to have anything either over or under processed. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cruise, Karen Sent: Thursday, October 07, 2010 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Equipment Purchase Questions Hello Histo Community, We are currently looking to purchase several items. I'm hoping someone can shed light on whether or not we are headed in the right direction. Processor: Initially we were leaning towards the Leica ASP 300, now I'm wondering if we are about to purchase to much processor for the needs of our lab. We process maybe 50 blocks per month. We are unable to use a microwave processor. We process about 95% breast tissue. Someone mentioned the TP1020. Has anyone any comments on this processor. We are also looking to purchase a ph meter and a fume adsorber, any recommendations ? Your responses will be greatly appreciated as we are looking to purchase before the end of the month. Thanks for all your help and suggestions, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcru...@path.wustl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Equipment Purchase Questions
I completely agree with Steve. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Thursday, October 07, 2010 11:21 AM To: Cruise, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Equipment Purchase Questions Karen, I would highly recommend Leica's Peloris processor. We use it for rapid tissue processing (2 hour processing time for cores and small specimens). It gives us the option of using one retort for rapid processing and the other for more conventional processing. We are also saving on reagents since we do not have to change out the entire processor weekly or twice per week. Peloris keeps track of the reagents and lets us know when one of them needs to be changed. We have been using our two units for a little over 9 months and we have yet to have anything either over or under processed. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cruise, Karen Sent: Thursday, October 07, 2010 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Equipment Purchase Questions Hello Histo Community, We are currently looking to purchase several items. I'm hoping someone can shed light on whether or not we are headed in the right direction. Processor: Initially we were leaning towards the Leica ASP 300, now I'm wondering if we are about to purchase to much processor for the needs of our lab. We process maybe 50 blocks per month. We are unable to use a microwave processor. We process about 95% breast tissue. Someone mentioned the TP1020. Has anyone any comments on this processor. We are also looking to purchase a ph meter and a fume adsorber, any recommendations ? Your responses will be greatly appreciated as we are looking to purchase before the end of the month. Thanks for all your help and suggestions, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcru...@path.wustl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Equipment Purchase Questions
I third that! I have had my Peloris for about 2 years. The key is thin, uniform sections. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Thursday, October 07, 2010 11:24 AM To: 'Feher, Stephen'; Cruise, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Equipment Purchase Questions I completely agree with Steve. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Thursday, October 07, 2010 11:21 AM To: Cruise, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Equipment Purchase Questions Karen, I would highly recommend Leica's Peloris processor. We use it for rapid tissue processing (2 hour processing time for cores and small specimens). It gives us the option of using one retort for rapid processing and the other for more conventional processing. We are also saving on reagents since we do not have to change out the entire processor weekly or twice per week. Peloris keeps track of the reagents and lets us know when one of them needs to be changed. We have been using our two units for a little over 9 months and we have yet to have anything either over or under processed. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cruise, Karen Sent: Thursday, October 07, 2010 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Equipment Purchase Questions Hello Histo Community, We are currently looking to purchase several items. I'm hoping someone can shed light on whether or not we are headed in the right direction. Processor: Initially we were leaning towards the Leica ASP 300, now I'm wondering if we are about to purchase to much processor for the needs of our lab. We process maybe 50 blocks per month. We are unable to use a microwave processor. We process about 95% breast tissue. Someone mentioned the TP1020. Has anyone any comments on this processor. We are also looking to purchase a ph meter and a fume adsorber, any recommendations ? Your responses will be greatly appreciated as we are looking to purchase before the end of the month. Thanks for all your help and suggestions, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcru...@path.wustl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] problems with staining IHC
If Erie is the manufacture of your Fisher slides then yes we have had the same problem with our IHC's. We use the Ventana Benchmark and have had this problem off and on for about 8 months. There seems to be no consistency with the quality of slides from Erie. We have just switched to the TruBond 200 slides which are distributed by STATLABS. These are working great for our IHC's. Sandra Sandra Esparza HT(ASCP)QIHC Lead Technologist Dell Children's Medical Center of Central Texas 512-324- x87061 sespa...@seton.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Thursday, October 07, 2010 10:53 AM To: sris...@mail.holyname.org; histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] problems with staining IHC Hi Mala, We've used the Fisher superfrost plus slides for years and have never had that issue, it sounds more like you need to add some Tween to your buffer. That will help the solutions spread along your slide. Hope that helps. Thanks, Timothy Higgins, HT(ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Thursday, October 07, 2010 9:32 AM To: histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslip Film
Hello Does anyone use a non-Sakura coverslip film on your Sakura coverslipper? My boss handed me a flyer from a company that sells film and it's a lot less money than the Sakura brand. Thanks, Paula Lab Manager Path Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Responses
Thanks so much for your quick responses .Let me elaborate on my specific needs. We are a research lab that deals mainly with breast tissue. I have been led to believe that microwave processing may not be the way to go because of our specimens are being used for RNA and DNA studies. A quick turn around time is not a concern of ours since we process and hold specimens for future use based upon requests throughout the US. Our main concern is not purchasing more processor than what we need since we only process maybe 5-10 cassettes per week. Thanks again, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcru...@path.wustl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p16 antibody
Hello all I am relatively new to IHC and my pathologist is requesting the p16 antibody, I have not been able to find it at any of the vendors we work with. I did a google search and saw that MTM labs has it but it seems as though they sell it as a kit. Does anyone know where I could find this antibody that I could use on the bond autostaining machine. Thank you, Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16 antibody
If you want the IVD form of the antibody you have to go with MTM labs, they hold the patent. We buy the kit and take out the antibody and use the detection for research. It works very very well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Carr [michelecar...@yahoo.com] Sent: Thursday, October 07, 2010 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody Hello all I am relatively new to IHC and my pathologist is requesting the p16 antibody, I have not been able to find it at any of the vendors we work with. I did a google search and saw that MTM labs has it but it seems as though they sell it as a kit. Does anyone know where I could find this antibody that I could use on the bond autostaining machine. Thank you, Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16 antibody
Hi Michele, Yes, it's true, only MTM labs sells it and they Sell it as a kit and they also sell it along with a negative control reagent. That's how I buy it. They have a license or something and no else can sell it now. Their cat# is 9518 and I think it's called the CINteck Histology V-kit, (you should double check with them.) They will make you open up an account... Good Luck Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Carr Sent: Thursday, October 07, 2010 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody Hello all I am relatively new to IHC and my pathologist is requesting the p16 antibody, I have not been able to find it at any of the vendors we work with. I did a google search and saw that MTM labs has it but it seems as though they sell it as a kit. Does anyone know where I could find this antibody that I could use on the bond autostaining machine. Thank you, Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 83, Issue 8
Another consideration as a few have mentioned could be with your automated stainer (if you use one). We have a DAKO autostainer and we found our staining had become very sporadic. When I did a repeat run manually, everything was beautiful. We found that a pump on the stainer was going and it wasn't washing all the reagents off the slide between steps. In addition the probe needed to be replaced as it was drawing up air into the lines and the volumes it was dispensing on the slides was variable from slide to slide depending on how much air was in the probe lines at any given time. If you do use automation, when was the last time the machine had a PM? I agree with the others that tween in your wash buffer is a must. Joanne Clark, HT Pathology Consultants of New Mexico -- Message: 1 Date: Thu, 7 Oct 2010 07:32:13 -0700 From: sris...@mail.holyname.org Subject: [Histonet] problems with staining IHC To: histonet-boun...@lists.utsouthwestern.edu, Histonet@lists.utsouthwestern.edu Message-ID: of6e74eb3d.a17b453b-on852577b5.004f018d-882577b5.005f9...@holyname.org Content-Type: text/plain; charset=US-ASCII Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Equipment purchases responses
Thanks so much for your quick responses .Let me elaborate on my specific needs. We are a research lab that deals mainly with breast tissue. I have been led to believe that microwave processing may not be the way to go because of our specimens are being used for RNA and DNA studies. A quick turn around time is not a concern of ours since we process and hold specimens for future use based upon requests throughout the US. Our main concern is not purchasing more processor than what we need since we only process maybe 5-10 cassettes per week. Thanks again, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcru...@path.wustl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslip Film
Once I tried one and it was harder, reacted less readily with xylene, and tended to peel-off easier. You could ask for a sample and try it. René J. --- On Thu, 10/7/10, Paula Lucas plu...@biopath.org wrote: From: Paula Lucas plu...@biopath.org Subject: [Histonet] Coverslip Film To: histonet@lists.utsouthwestern.edu Date: Thursday, October 7, 2010, 12:45 PM Hello Does anyone use a non-Sakura coverslip film on your Sakura coverslipper? My boss handed me a flyer from a company that sells film and it's a lot less money than the Sakura brand. Thanks, Paula Lab Manager Path Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone IHC
Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval (pressure cooker for 20mins) most of the boney part of the tissue comes off and the marrow and muscle stays put! The sections are cut onto superfrost plus slides. Any help would be much appreciated, thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ECM stain for growing cartilage
Hello Everyone Is anyone out there aware of an ECM stain that can be used on growing tissue culture cells in a hydrogel. What I'm really looking for is a live stain I can use on the whole gel and see with a light microscope. I want to get a general idea of the amount of ECM that is growing. So it could just be a general bulk ECM (proteoglycan or collagen) stain that will show a contrast from the gel. And I want to be able to return the gel to the incubator and do the stain again multiple days later. I have searched extensively for something that would do this and I have come up with nothing. Any advice is appreciated and thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bone IHC
We lower the temp of retrieval to 70C for 2 hours and have good success with that. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa J. Phelan Sent: Thursday, October 07, 2010 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone IHC Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval (pressure cooker for 20mins) most of the boney part of the tissue comes off and the marrow and muscle stays put! The sections are cut onto superfrost plus slides. Any help would be much appreciated, thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bone IHC
If you have a bone that will require IHC, you will have to make sure that the Gross section is no more than 1.5 mm thick and decalcify it in EDTA. The you have to make sure that the infiltration is complete. These are the 2 initial factors. Later you will have to section it as thin as you can, place the sections in (+) slides let them drain completely before going into the oven. Extra time in the oven is not really required if the aforementioned steps are done. Then add enough dishwasher soap to the HIER solution to get a solution of 2% and you will dewax and retrieve the antigen simultaneously. The sections will survive to complete the IHC procedure.René J. --- On Thu, 10/7/10, Vanessa J. Phelan vjp2...@columbia.edu wrote: From: Vanessa J. Phelan vjp2...@columbia.edu Subject: [Histonet] Bone IHC To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, October 7, 2010, 3:13 PM Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval (pressure cooker for 20mins) most of the boney part of the tissue comes off and the marrow and muscle stays put! The sections are cut onto superfrost plus slides. Any help would be much appreciated, thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bone IHC
I've been experimenting with different ways to solve this problem myself. I fix my tissues in 10% zinc buffered formalin and decalcify in formic acid for 72 hours, followed by embedding and cutting 5 uM sections. From trial and error, I've determined that incubating the slides flat on a slide warmer at 37C (or in my case, the bottom of an unused bacterial incubator) can prevent nearly all the detachment you observe but it's time dependent. I think the problem is that the sections get small amounts of water underneath them when you scoop them up off the water surface during sectioning and during HIER, that water boils and shears off the slide. If you leave the slides overnight, the slides were get destroyed during HIER. However, if you leave them for a week, they tend to be nearly untouched even at 95C for 10 mins. I'm currently in the process of determining if a few days is enough time. Adam On Thu, Oct 7, 2010 at 2:20 PM, Liz Chlipala l...@premierlab.com wrote: We lower the temp of retrieval to 70C for 2 hours and have good success with that. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa J. Phelan Sent: Thursday, October 07, 2010 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone IHC Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval (pressure cooker for 20mins) most of the boney part of the tissue comes off and the marrow and muscle stays put! The sections are cut onto superfrost plus slides. Any help would be much appreciated, thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Supervisor Job in Texas!
Hi Histonet Members I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working with a client in the Midland/Odessa Texas area to locate a Histology Supervisor. This 350+ bed community teaching hospital system has a very strong reputation in area. My client is looking for someone who is HT or HTL certified, has 5 + years for histology experience, and prefers someone with supervisory or lead experience. This hospital system offers a very competitive base salary/hourly rate, an outstanding benefits and retirement package, and relocation assistance. Please email me asap if you are interested in more details. Email ali...@ka-recruiting.com. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Laboratory Opportunities: Histotechnologist: 1. Histotech - Syracuse NY 2. Histology Manager - Michigan 3. Histotech - NYC 4. Histology Supervisor - GA 5. Histotech - NV (Must have Bachelors Degree) 6. Nashville, TN - Histotech 7. Histotech - Cape Cod, MA 8. Histology Supervisor - TX 9. General Manager of Anatomic Pathology - NJ If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cassette labelers
I'm advising a locum tenens client of mine about acquiring a cassette labeler. The only one I'm familiar with is Thermo Scientific's Cassette MicroWriter. Some questions for HistoNet: 1) A JCAHO inspector informed them that a cassette labeler will soon be required. Does anyone know if this is in fact the case? 2) Will the labeler print patients' names, or other second identifiers such as JCAHO now requires? 3) Are these labelers available in the used instrument market? 4) Is there any competition, or is the Thermo product all there is? 5) How well do these labelers withstand use? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Equipment purchases responses
Hi Karen, I am in a the same Research situation as you. If you are sure that all you will need is small (100) tissue cassette processings, the Leica TP1020 should suffice. We call them Dip-and-Dunks, as the tissue carousels from bucket to bucket. It is economical on reagents and is easy to use. Plan on 12+ hour processing runs for tissue with fat. Get the extra paraffin bucket (3) if you can. It is not a necessity for the routine processing run, but it is 'Handy' to have. However for fatty tissues like breast, the tissue processing is superior in vacuum infiltration units like the Leica ASP300S or a Tissue Tek VIP series. If you have the money, I recommend one of these. Both are work-horses and will give you practically no trouble. However, you will hear new terms like Warm Water Flush, Clean the Retort, and Annual preventive maintenance. You will also triple your Waste reagents compared to the TP1020. I have a Leica ASP300. It was a refurbished unit, which I regret every so often, but it was necessary to procure due to money and time. Mostly money. It has saved my bu** more often than not. I can offer some recommendations for vendors like this if you wish. I also have access to the TP1020, ASP300S, Peloris, and the Tissue Teks 300, 5, and 6. I prefer the Tissue Tek 6, but all units have worked well for me. I have read other recommendations for the Leica Peloris or some variety of microwave tissue processor. These processors are absolutely fantastic and fast, but I have the same red-flags as you with RNA/DNA damage.No microwaves. The Peloris, in theory, will not cause further RNA/DNA damage, but it is advertised as Faster Than Microwaves, so I would use caution pursuing this model until someone documents what it is doing to the nucleotides. Also, the Peloris is more expensive and it's 600 block capacity is too big for my (your?) needs. As for your portable fume hood and pH meter. pH meters range from cheap to super-expensive. The pocket version is cheap but falls into disrepair quickly. The bench-top models, requiring pH Standard calibrations and probe care, are great, but a pain to maintain and expensive to buy. You need to define what you need it for. Translational Pathology? Perhaps you can borrow? It seems to be a waste to have an expensive pH meter if (for example) you only measure buffers once a week. We have a Checkmate from Cardinal scientific, but I think this model has been discontinued. Fume hood; If you cannot get your facilities folks to put a real hood in, try Labconco or Thermo. Get one that can be used as a Laminar Flow (air filter parallel to desk), as formalin and xylene fumes lay close to the desk-top. There are lots of brands with different kinds and sizes of hoods. I believe that filtered hoods are not very efficient at cleaning the air of formalin (potassium permanganate filter) or xylene (carbon), simply because there is not enough air exchange. An example of our 36x24x30 self contained hood is in the link below: http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=82010-736inE=1highlight=82010-736 Hope this helps, Hugh Luk, HTL (ASCP) Pathology shared resource lab manager 1236 Lauhala street, Honolulu, HI 96813 Date: Thu, 7 Oct 2010 13:00:47 -0500 From: kcru...@path.wustl.edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Equipment purchases responses Thanks so much for your quick responses .Let me elaborate on my specific needs. We are a research lab that deals mainly with breast tissue. I have been led to believe that microwave processing may not be the way to go because of our specimens are being used for RNA and DNA studies. A quick turn around time is not a concern of ours since we process and hold specimens for future use based upon requests throughout the US. Our main concern is not purchasing more processor than what we need since we only process maybe 5-10 cassettes per week. Thanks again, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcru...@path.wustl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
