Re: [Histonet] Eosin

2010-10-19 Thread Massimo
Yes. Especially when you watch into a test tube an 1% eosin solution and it is 
going to be diluted with distilled water. At that point it can be used for the 
staining of histological slices.

Best Regards,

Massimo




Da: Rene J Buesa rjbu...@yahoo.com
A: histonet@lists.utsouthwestern.edu; sgoe...@xbiotech.com
Inviato: Lun 18 ottobre 2010, 17:51:43
Oggetto: Re: [Histonet] Eosin

Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if 
you 
observe a solution with transmitted light.
René J.

--- On Mon, 10/18/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote:


From: sgoe...@xbiotech.com sgoe...@xbiotech.com
Subject: [Histonet] Eosin
To: histonet@lists.utsouthwestern.edu
Date: Monday, October 18, 2010, 10:53 AM



   Once  upon a time I heard that eosin fluoresces is this true? = ; What
   color  does it show up?  Could this be used as a sort of back gr= ound
   stain so you can tell exactly how many cells are in the field?
   Sarah Goebel, B.A., HT (ASCP)
   Histotechnician
   = = XBiotech USA Inc.
   8201 East Riversid= e Dr. Bldg 4 Suite 100
   Austin, Texas=   78744
   (512)386-2907
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[Histonet] hi-profile blades

2010-10-19 Thread Margiotta, Michele
Hi All,

One of my techs loves using hi-profile blades but recently she has been having 
problems with the quality and consistency of the blades.  One will cut great 
and the next one will give her problems cutting.  I'd appreciate any 
suggestions for blade type/company that histotechs are happy with so we can try 
them out.

Thanks,
Michele Margiotta
BMHMC
Histology Supervisor
631-654-7192




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[Histonet] hi-profile blades

2010-10-19 Thread Wendy Bridges
I have always found Accu-Cut blades by Sakura to be the best blades.

 

Wendy Bridges, HT (ASCP)

Lead Tech Specialist

Cape Fear Valley Hospital

Fayetteville, NC

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[Histonet] 2 Histoech positions open at PBM Dallas Tx

2010-10-19 Thread Jose Taramona
Looking for 2 excellent, reliable histotechs for routine histology
department.
Also looking for an excellent histotech with Immuno experience.
Competitive pay. Interested?
Need more information contact Jose Taramona at:
Email: jo...@pbmlabs.com
Ph# 214-818-9138
Fax# 214-818-9170 attn: Jose
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RE: [Histonet] Un related to actual histology

2010-10-19 Thread sgoebel
Roll Roll Roll in the hay =)  One of my all time favorite movies!!


Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907




 Original Message 
Subject: Re: [Histonet] Un related to actual histology
From: Emily Sours talulahg...@gmail.com
Date: Mon, October 18, 2010 3:37 pm
To: Ann Bennett ann.bennett...@yahoo.com,
histonet@lists.utsouthwestern.edu

I don't know if you're a fan of Mystery Science Theater 3000, but you
could
be Dr Clayton Forrester!
Or you could be Dexter or House. Dexter would be fun.
Better yet, be Frankenstein, the doctor. That'll confuse people who
aren't
aware the monster had no name.
Or even, FrankenSTEEN. Stab a scapel in your thigh for full effect. Also
bring a recording of horses neighing so you can say Frau Bleuker!

Emily
--
Outside of a dog, a book is man's best friend. Inside of a dog it's too
dark
to read.
--Groucho Marx


On Mon, Oct 18, 2010 at 5:24 PM, Ann Bennett
ann.bennett...@yahoo.comwrote:

 Hello everyone!

 Our lab is having a laboratory related costume contest for Halloween. I'd
 love to hear your ideas!



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[Histonet] kappa and lambda by CISH

2010-10-19 Thread Kuhnla, Melissa
Hello All,
Anyone currently running Kappa and Lambda by CISH?  I am in the process
of validating the Ventana products.  Due to the ASR status of these
products the vendor is extremely 'tight lipped'.  I have the stains
worked up and have completed a 25 case validation. Does anyone know if
this test is not to be used on decaled specimens??
Thank you


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[Histonet] TN - Great HT Opprotunity

2010-10-19 Thread Hale, Meredith
 

Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mh...@carisls.com

 

 

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mh...@carisls.com 

 

 

 

 

 

 

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[Histonet] DAKO Coverslipper

2010-10-19 Thread Kim . Donadio
Has anyone ever used the Dako coverslipper? If so, what did you think? 


Thanks



Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

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[Histonet] RE: kappa and lambda by CISH

2010-10-19 Thread McMahon, Loralee A
I have been using the RISH from Biocare and you can use it on Decaled 
specimens.  they have a slightly different protocol (pretreatment) but we have 
found that you don't need to adjust with our form of decal.
Not completely finished with the validation process on these, but that is what 
we have found so far. 


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa 
[melissa.kuh...@chsli.org]
Sent: Tuesday, October 19, 2010 10:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] kappa and lambda by CISH

Hello All,
Anyone currently running Kappa and Lambda by CISH?  I am in the process
of validating the Ventana products.  Due to the ASR status of these
products the vendor is extremely 'tight lipped'.  I have the stains
worked up and have completed a 25 case validation. Does anyone know if
this test is not to be used on decaled specimens??
Thank you


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AW: [Histonet] kappa and lambda by CISH

2010-10-19 Thread Gudrun Lang
I've found, that kappa and lambda mRNA CISH is reliable for specimen
decalcified with 5-10% formic acid. Decal with harsher acids like
hydrochloric acid should be validated, because any acid will cause
degradation of nucleic acids. We use the CISH kit from Zytovision.
Journal of Histotechnol 33(1):9-13, 2010

Gudrun Lang

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Kuhnla,
Melissa
Gesendet: Dienstag, 19. Oktober 2010 16:50
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] kappa and lambda by CISH

Hello All,
Anyone currently running Kappa and Lambda by CISH?  I am in the process
of validating the Ventana products.  Due to the ASR status of these
products the vendor is extremely 'tight lipped'.  I have the stains
worked up and have completed a 25 case validation. Does anyone know if
this test is not to be used on decaled specimens??
Thank you


The information in this e-mail, and any attachments therein, is confidential
and for use by the intended addressee only. If this message is received by
you in error please do not disseminate or read further. Please reply to the
sender that you have received the message in error, then delete the message.
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and attachments for viruses, it does not guarantee that either are
virus-free and accepts no liability for any damage sustained as a result of
viruses. Thank you.

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RE: [Histonet] hi-profile blades

2010-10-19 Thread Bernice Frederick
I've been using Accu-edge blades from Sakura and have found nothing to equal
them. We use low profile here, but high are the same quality.


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margiotta,
Michele
Sent: Tuesday, October 19, 2010 7:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] hi-profile blades

Hi All,

One of my techs loves using hi-profile blades but recently she has been
having problems with the quality and consistency of the blades.  One will
cut great and the next one will give her problems cutting.  I'd appreciate
any suggestions for blade type/company that histotechs are happy with so we
can try them out.

Thanks,
Michele Margiotta
BMHMC
Histology Supervisor
631-654-7192




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Re: [Histonet] hi-profile blades

2010-10-19 Thread Drew Meyer
I agree with Bernice... I've used both the low and high profile Sakura
AccuEdge nothing comes close to their quality and consistency.

Drew

On Tue, Oct 19, 2010 at 11:44, Bernice Frederick 
b-freder...@northwestern.edu wrote:

 I've been using Accu-edge blades from Sakura and have found nothing to
 equal
 them. We use low profile here, but high are the same quality.


 Bernice Frederick HTL (ASCP)
 Northwestern University
 Pathology Core Facility
 ECOGPCO-RL
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margiotta,
 Michele
 Sent: Tuesday, October 19, 2010 7:14 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] hi-profile blades

 Hi All,

 One of my techs loves using hi-profile blades but recently she has been
 having problems with the quality and consistency of the blades.  One will
 cut great and the next one will give her problems cutting.  I'd appreciate
 any suggestions for blade type/company that histotechs are happy with so we
 can try them out.

 Thanks,
 Michele Margiotta
 BMHMC
 Histology Supervisor
 631-654-7192




 DISCLAIMER:
 This e-mail and any files transmitted with it are confidential and are
 intended solely for the use of the individual or entity to which they are
 addressed.  This communication may contain material protected by the
 attorney-client privilege.  If you are not the intended recipient or the
 person responsible for delivering the e-mail to the intended recipient, be
 advised that you have received this e-mail in error and that any use,
 dissemination, forwarding, printing, or copying of this e-mail is strictly
 prohibited.  If you have received this e-mail in error, please immediately
 notify the sender via return e-mail or call Brookhaven Memorial Hospital
 Medical Center at (631) 654-7282.

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Re: [Histonet] kappa and lambda by CISH

2010-10-19 Thread Mark Tarango
Hi Melissa,

We use it on bone marrow biopsies all the time on the Ventana XT.  We made
sure to include BMBXs in the validation.  We do get strange staining from
time to time, so it's particularily important to look at the negative
control for these.

Mark Tarango

On Tue, Oct 19, 2010 at 7:49 AM, Kuhnla, Melissa
melissa.kuh...@chsli.orgwrote:

 Hello All,
 Anyone currently running Kappa and Lambda by CISH?  I am in the process
 of validating the Ventana products.  Due to the ASR status of these
 products the vendor is extremely 'tight lipped'.  I have the stains
 worked up and have completed a 25 case validation. Does anyone know if
 this test is not to be used on decaled specimens??
 Thank you


 The information in this e-mail, and any attachments therein, is
 confidential and for use by the intended addressee only. If this message is
 received by you in error please do not disseminate or read further. Please
 reply to the sender that you have received the message in error, then delete
 the message. Although Catholic Health Services of Long Island attempts to
 sweep e-mail  and attachments for viruses, it does not guarantee that either
 are virus-free and accepts no liability for any damage sustained as a result
 of viruses. Thank you.

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RE: [Histonet] hi-profile blades

2010-10-19 Thread Anatoli Gleiberman
We use AccuEdge low and high profile from Sakura and several low and
high profile blades from Sturkey (Silver, Gold and Extremus) and found
that Sturkey knifes are as good as AccuEdge (both for paraffin and cryo
sections) and significantly cheaper if you buy it directly from the
company. 

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: agleiber...@cbiolabs.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew
Meyer
Sent: Tuesday, October 19, 2010 11:48 AM
To: Bernice Frederick
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] hi-profile blades

I agree with Bernice... I've used both the low and high profile Sakura
AccuEdge nothing comes close to their quality and consistency.

Drew

On Tue, Oct 19, 2010 at 11:44, Bernice Frederick 
b-freder...@northwestern.edu wrote:

 I've been using Accu-edge blades from Sakura and have found nothing to
 equal
 them. We use low profile here, but high are the same quality.


 Bernice Frederick HTL (ASCP)
 Northwestern University
 Pathology Core Facility
 ECOGPCO-RL
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Margiotta,
 Michele
 Sent: Tuesday, October 19, 2010 7:14 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] hi-profile blades

 Hi All,

 One of my techs loves using hi-profile blades but recently she has
been
 having problems with the quality and consistency of the blades.  One
will
 cut great and the next one will give her problems cutting.  I'd
appreciate
 any suggestions for blade type/company that histotechs are happy with
so we
 can try them out.

 Thanks,
 Michele Margiotta
 BMHMC
 Histology Supervisor
 631-654-7192




 DISCLAIMER:
 This e-mail and any files transmitted with it are confidential and are
 intended solely for the use of the individual or entity to which they
are
 addressed.  This communication may contain material protected by the
 attorney-client privilege.  If you are not the intended recipient or
the
 person responsible for delivering the e-mail to the intended
recipient, be
 advised that you have received this e-mail in error and that any use,
 dissemination, forwarding, printing, or copying of this e-mail is
strictly
 prohibited.  If you have received this e-mail in error, please
immediately
 notify the sender via return e-mail or call Brookhaven Memorial
Hospital
 Medical Center at (631) 654-7282.

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Re: [Histonet] hi-profile blades

2010-10-19 Thread Langenberg, Stacey
I too swear by Sturkey. It is the only blade my lab buys and uses. My techs 
could use any blade they want but they all love Sturkey!

Stacey Langenberg
Sent via BlackBerry from T-Mobile

-Original Message-
From: Anatoli Gleiberman agleiber...@cbiolabs.com
Sender: histonet-boun...@lists.utsouthwestern.edu
histonet-boun...@lists.utsouthwestern.edu
Date: Tue, 19 Oct 2010 10:00:01 
To: Histonet@lists.utsouthwestern.eduHistonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] hi-profile blades

We use AccuEdge low and high profile from Sakura and several low and
high profile blades from Sturkey (Silver, Gold and Extremus) and found
that Sturkey knifes are as good as AccuEdge (both for paraffin and cryo
sections) and significantly cheaper if you buy it directly from the
company. 

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: agleiber...@cbiolabs.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew
Meyer
Sent: Tuesday, October 19, 2010 11:48 AM
To: Bernice Frederick
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] hi-profile blades

I agree with Bernice... I've used both the low and high profile Sakura
AccuEdge nothing comes close to their quality and consistency.

Drew

On Tue, Oct 19, 2010 at 11:44, Bernice Frederick 
b-freder...@northwestern.edu wrote:

 I've been using Accu-edge blades from Sakura and have found nothing to
 equal
 them. We use low profile here, but high are the same quality.


 Bernice Frederick HTL (ASCP)
 Northwestern University
 Pathology Core Facility
 ECOGPCO-RL
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Margiotta,
 Michele
 Sent: Tuesday, October 19, 2010 7:14 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] hi-profile blades

 Hi All,

 One of my techs loves using hi-profile blades but recently she has
been
 having problems with the quality and consistency of the blades.  One
will
 cut great and the next one will give her problems cutting.  I'd
appreciate
 any suggestions for blade type/company that histotechs are happy with
so we
 can try them out.

 Thanks,
 Michele Margiotta
 BMHMC
 Histology Supervisor
 631-654-7192




 DISCLAIMER:
 This e-mail and any files transmitted with it are confidential and are
 intended solely for the use of the individual or entity to which they
are
 addressed.  This communication may contain material protected by the
 attorney-client privilege.  If you are not the intended recipient or
the
 person responsible for delivering the e-mail to the intended
recipient, be
 advised that you have received this e-mail in error and that any use,
 dissemination, forwarding, printing, or copying of this e-mail is
strictly
 prohibited.  If you have received this e-mail in error, please
immediately
 notify the sender via return e-mail or call Brookhaven Memorial
Hospital
 Medical Center at (631) 654-7282.

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information.  If you received this communication in error, please contact the 
sender immediately and destroy the material in its entirety, whether electronic 
or hard copy.  This communication may contain nonpublic information about 
individuals and businesses subject to the restrictions of the 
Gramm-Leach-Bliley Act.  You may not directly or indirectly reuse or redisclose 
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[Histonet] CLIA Inspection

2010-10-19 Thread Weems, Joyce
Would someone who has been CLIA inspected, please contact me privately? Thanks, 
j


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


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[Histonet] Fwd: IHC coverglassing

2010-10-19 Thread Jen Campbell


Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

- Forwarded Message -
From: Jen Campbell jcampbell_...@frontier.com
To: histonet histonet-boun...@lists.utsouthwestern.edu
Sent: Tuesday, October 19, 2010 11:16:38 AM
Subject: IHC coverglassing

Greetings to all the histonet followers!!

A question was recently brought forth in the lab I work at. Can non-automated 
IHC slides be dehydrated and cleared(xylene substitute) after staining and be 
placed on an automated coverglasser?

Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

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[Histonet] High Profile Blades

2010-10-19 Thread Parker, Helayne
I used to love the TBS blades (green- I think), but we use low profile here. We 
use Thermo and Accu Edge.

Helayne Parker
CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or 
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[Histonet] Frozen sections

2010-10-19 Thread Maria Katleba
Question:  When doing frozen section on a lung mass (not fatty- just regular), 
and it chatters, what is the cause?

The cryostat was set at -26, we used the normal OCT media, and cut at 
5microns.

I prefer not so cold (like -23 to -24)   and I cut sections at 4, 5 , and even 
6 to see if it would help... still chatter!The pathologist said it looked 
all necrotic-like!!

Without knowing for sure if the tissue's morphology is due to its real state 
(ie- full of cancer) or a cause of freezing artifact, I need some help from any 
histotech that is a Frozen Section Guru :)

Maria Katleba MS HT(ASCP)
Pathology Dept. Mgr
Queen of the Valley Medical Center
1000 Trancas Street
Napa CA 94558
(707) 252-4411 x3689 direct
(707) 226-4385 pager
(707) 294-9229 cell- anytime


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RE: [Histonet] Fwd: IHC coverglassing

2010-10-19 Thread Thomas, Nancy
Hi Jen,
We do it that way routinely.  No problems.
Nancy

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jen Campbell
Sent: Tuesday, October 19, 2010 12:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: IHC coverglassing




Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

- Forwarded Message -
From: Jen Campbell jcampbell_...@frontier.com
To: histonet histonet-boun...@lists.utsouthwestern.edu
Sent: Tuesday, October 19, 2010 11:16:38 AM
Subject: IHC coverglassing

Greetings to all the histonet followers!!

A question was recently brought forth in the lab I work at. Can non-automated 
IHC slides be dehydrated and cleared(xylene substitute) after staining and be 
placed on an automated coverglasser?

Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

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RE: [Histonet] Fwd: IHC coverglassing

2010-10-19 Thread Houston, Ronald
absolutely

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jen Campbell
Sent: Tuesday, October 19, 2010 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: IHC coverglassing



Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

- Forwarded Message -
From: Jen Campbell jcampbell_...@frontier.com
To: histonet histonet-boun...@lists.utsouthwestern.edu
Sent: Tuesday, October 19, 2010 11:16:38 AM
Subject: IHC coverglassing

Greetings to all the histonet followers!!

A question was recently brought forth in the lab I work at. Can non-automated 
IHC slides be dehydrated and cleared(xylene substitute) after staining and be 
placed on an automated coverglasser?

Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

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[Histonet] FX

2010-10-19 Thread Tench, Bill
it's too cold.  set your cryostat at 20.  put your tumb on the block
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
bill.te...@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

[None] made the following annotations
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RE: [Histonet] Fwd: IHC coverglassing

2010-10-19 Thread sgoebel
Absolutely!!  After the buffer rinse (after the counterstain), rinse in
water, then 95, then 100, then xylene, and coverslip away!!  I do it
everyday and it works fine =)


Sarah Goebel, B.A., HT (ASCP)

Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907




 Original Message 
Subject: [Histonet] Fwd: IHC coverglassing
From: Jen Campbell jcampbell_...@frontier.com
Date: Tue, October 19, 2010 10:16 am
To: histonet@lists.utsouthwestern.edu



Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

- Forwarded Message -
From: Jen Campbell jcampbell_...@frontier.com
To: histonet histonet-boun...@lists.utsouthwestern.edu
Sent: Tuesday, October 19, 2010 11:16:38 AM
Subject: IHC coverglassing

Greetings to all the histonet followers!!

A question was recently brought forth in the lab I work at. Can
non-automated IHC slides be dehydrated and cleared(xylene substitute)
after staining and be placed on an automated coverglasser?

Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

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RE: [Histonet] Frozen sections

2010-10-19 Thread sgoebel
Chatter can also be caused by your blade angle.  Try making the angle
more obtuse, just don't over do it or you will get venitian (sp?) blind.
 The temperature is also a little cold.  Try putting your thumb on the
chuck, on top of the tissue, for a few sections before cutting and see
if that helps any.  If the tissue is necrotic, it's kind of like
fat...not much you can do?
Good Luck!!

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907




 Original Message 
Subject: [Histonet] Frozen sections
From: Maria Katleba maria.katl...@stjoe.org
Date: Tue, October 19, 2010 10:27 am
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu

Question: When doing frozen section on a lung mass (not fatty- just
regular), and it chatters, what is the cause?

The cryostat was set at -26, we used the normal OCT media, and cut at
5microns.

I prefer not so cold (like -23 to -24) and I cut sections at 4, 5 , and
even 6 to see if it would help... still chatter! The pathologist said it
looked all necrotic-like!!

Without knowing for sure if the tissue's morphology is due to its real
state (ie- full of cancer) or a cause of freezing artifact, I need some
help from any histotech that is a Frozen Section Guru :)

Maria Katleba MS HT(ASCP)
Pathology Dept. Mgr
Queen of the Valley Medical Center
1000 Trancas Street
Napa CA 94558
(707) 252-4411 x3689 direct
(707) 226-4385 pager
(707) 294-9229 cell- anytime


Notice from St. Joseph Health System:
Please note that the information contained in this message may be
privileged and confidential and protected from disclosure.



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[Histonet] fx

2010-10-19 Thread Tench, Bill
that's suppose to be  put your thumb on the block. 
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
bill.te...@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

[None] made the following annotations
-
Confidential E-Mail: This e-mail is intended only for the person or entity to 
which it is addressed, and may contain information that is privileged, 
confidential, or otherwise protected from disclosure. Dissemination, 
distribution, or copying of this e-mail or the information herein by anyone 
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Re: [Histonet] hi-profile blades

2010-10-19 Thread Jennifer MacDonald
Is the inconsistency between brands of blades, the same brand, or within 
an individual dispenser?





Margiotta, Michele mmargio...@bmhmc.org 
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/19/2010 05:18 AM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] hi-profile blades






Hi All,

One of my techs loves using hi-profile blades but recently she has been 
having problems with the quality and consistency of the blades.  One will 
cut great and the next one will give her problems cutting.  I'd appreciate 
any suggestions for blade type/company that histotechs are happy with so 
we can try them out.

Thanks,
Michele Margiotta
BMHMC
Histology Supervisor
631-654-7192




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[Histonet] Tissue Processors

2010-10-19 Thread Matt Brooks
Hello All,

I am in the process of developing the Histology budget for next three
years.  There have been some positive posts about a few processors
recently; but if there are more opinions out there (and I know that
there are) please let me know.  At NSH I had the opportunity to view and
hear about the most of the tissue processors on the market.  There are
several features that I need to consider price, reliability, ease of
use/maintenance, timely and quality service, and processing TAT, just to
name a few.  Fellow Histology professionals you can email/contact me
directly and your input is greatly appreciated.

Thank you,   

Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbro...@incytepathology.com
509-892-2744


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Re: [Histonet] Cassette Marking

2010-10-19 Thread Kim . Donadio
IF I had to. 

pencil. And I am very funny about making sure it has a nice sharp dark 
lead. 



Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



Nita Searcy nsea...@swmail.sw.org 
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/19/2010 01:22 PM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] Cassette Marking






** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens 
? Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street 
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438

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RE: [Histonet] Cassette Marking

2010-10-19 Thread Maria Katleba
Try www.MercedesMedical.com   item # MER MARKERcalled Platinum Marker

These pens are dark and keep their colour,  pens outlast the Secureline marker 
and this company is very competitive with the price of this marker

Maria Katleba MS HT(ASCP)
Pathology Dept. Mgr
Queen of the Valley Medical Center
1000 Trancas Street
Napa CA 94558
(707) 252-4411 x3689 direct
(707) 226-4385 pager
(707) 294-9229 cell- anytime

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Tuesday, October 19, 2010 11:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking
Importance: High

** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens ? 
Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438


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Please note that the information contained in this message may be privileged 
and confidential and protected from disclosure.



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Re: [Histonet] Cassette Marking

2010-10-19 Thread Karen Sly
We use Cancer Diagnostics,
Moist Mark Plus. 

Karen Sly
Histology Dept.
Central Michigan Community Hospital
Mount Pleasant, Michigan

Please consider the environment before printing this e-mail 

 Please consider the environment before printing this e-mail

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RE: [Histonet] Cassette Marking

2010-10-19 Thread Sherwood, Margaret
The marking pencils work fine and we just ordered some marking pens from StatLab
which work great. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Tuesday, October 19, 2010 2:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking
Importance: High

** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens ?
Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street 
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


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RE: [Histonet] Cassette Marking

2010-10-19 Thread Jacqueline Farnsworth
Surgipath  01880
Permanent Ink . Ultra fine point. Non-toxic

Jacqueline Farnsworth
Anatomic Pathology, Tech III
Diagnostic Scientific Centre
Calgary Laboratory Services

Phone: 403-770-3588
Pager: 403-212-8223  X07630

P Please consider the environment before printing this email.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret  
[msherw...@partners.org]
Sent: October 19, 2010 12:38 PM
To: Nita Searcy; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cassette Marking

The marking pencils work fine and we just ordered some marking pens from StatLab
which work great.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Tuesday, October 19, 2010 2:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking
Importance: High

** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens ?
Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
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dispose of the e-mail.


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RE: [Histonet] Cassette Marking

2010-10-19 Thread Maria Katleba
Yes- I love Statlab! These marking pencils are great

Maria

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, 
Margaret
Sent: Tuesday, October 19, 2010 11:38 AM
To: Nita Searcy; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cassette Marking

The marking pencils work fine and we just ordered some marking pens from StatLab
which work great.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Tuesday, October 19, 2010 2:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking
Importance: High

** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens ?
Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


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RE: [Histonet] Cassette Marking

2010-10-19 Thread sgoebel
I've used this one too, the tip is very tiny, but the pen is awesome!

Sarah Goebel, B.A., HT (ASCP)

Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907




 Original Message 
Subject: RE: [Histonet] Cassette Marking
From: Maria Katleba maria.katl...@stjoe.org
Date: Tue, October 19, 2010 11:34 am
To: Nita Searcy nsea...@swmail.sw.org,
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu

Try www.MercedesMedical.com item # MER MARKER called Platinum Marker

These pens are dark and keep their colour, pens outlast the Secureline
marker and this company is very competitive with the price of this
marker

Maria Katleba MS HT(ASCP)
Pathology Dept. Mgr
Queen of the Valley Medical Center
1000 Trancas Street
Napa CA 94558
(707) 252-4411 x3689 direct
(707) 226-4385 pager
(707) 294-9229 cell- anytime

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita
Searcy
Sent: Tuesday, October 19, 2010 11:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking
Importance: High

** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette
pens ? Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438


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Please note that the information contained in this message may be
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RE: [Histonet] Cassette Marking

2010-10-19 Thread Sherwood, Margaret
Nita,

I would say you have quite a choice!  For the longest time, we had so much
trouble with so many pens, we went to the pencil for the cassettes which are the
best.  But we still needed pens for the slides; that's when we switched to
StatLab.  We still use the pencil for the cassettes.

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Tuesday, October 19, 2010 2:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking
Importance: High

** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens ?
Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street 
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
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[Histonet] Plain Vanilla Autostainer?

2010-10-19 Thread Johnson, Kevin
I am a simple man in a simple lab with simple needs.  I work in a multiple-lab 
research environment, typically providing paraffin/frozen sections + HE to 
individuals who then perform their own manual IHC.  A simple man, yes, but also 
a busy man, who needs to be freed from staining dishes in order to pursue other 
tasks.

What we need: (1) Deparaffinization only; (2) Deparaffinization -- HE; (3) 
HE only system, preferably with (4) a relatively small, preferably benchtop 
footprint (current unit lives in a 30 X 33 X 34 vented workstation).  
Automatic coverslipping extremely optional.

What we have: Thermo Shandon Varistain 24-4, which performed these mundane 
tasks admirably until going electronically insane.  It's been denied a service 
contract, the production line has been discontinued and the writing is on the 
wall.

What seem to be available:  Special staining systems with a whole lot of bells 
and whistles, designed primarily for a high-throughput hospital setting.

Is there anything out there that would serve my needs, even if it is a fancy 
system that would be slumming it here?  Or is mine just too obsolete a market?  
Price up to $15K, slightly negotiable.  (Bench space only slightly negotiable; 
floor model if I must.)

Many thanks,

Kevin Johnson
University of Miami
Diabetes Research Institute
Miami, FL
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RE: [Histonet] Plain Vanilla Autostainer?

2010-10-19 Thread sgoebel
What about just a simple old school ski stainer?

Sarah Goebel, B.A., HT (ASCP)

Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907




 Original Message 
Subject: [Histonet] Plain Vanilla Autostainer?
From: Johnson, Kevin kjohn...@med.miami.edu
Date: Tue, October 19, 2010 11:50 am
To: 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu

I am a simple man in a simple lab with simple needs. I work in a
multiple-lab research environment, typically providing paraffin/frozen
sections + HE to individuals who then perform their own manual IHC. A
simple man, yes, but also a busy man, who needs to be freed from
staining dishes in order to pursue other tasks.

What we need: (1) Deparaffinization only; (2) Deparaffinization -- HE;
(3) HE only system, preferably with (4) a relatively small, preferably
benchtop footprint (current unit lives in a 30 X 33 X 34 vented
workstation). Automatic coverslipping extremely optional.

What we have: Thermo Shandon Varistain 24-4, which performed these
mundane tasks admirably until going electronically insane. It's been
denied a service contract, the production line has been discontinued and
the writing is on the wall.

What seem to be available: Special staining systems with a whole lot of
bells and whistles, designed primarily for a high-throughput hospital
setting.

Is there anything out there that would serve my needs, even if it is a
fancy system that would be slumming it here? Or is mine just too
obsolete a market? Price up to $15K, slightly negotiable. (Bench space
only slightly negotiable; floor model if I must.)

Many thanks,

Kevin Johnson
University of Miami
Diabetes Research Institute
Miami, FL
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RE: [Histonet] Cassette Marking

2010-10-19 Thread Kim . Donadio
Yes, that's the reason we chose pencil's. We would have so many different 
marking pens around, some would wash off or smudge. One of the bad ones 
would end up in a drawer and get used later on down the line giving us a 
major headache. So pencil it is if we ever needed to. We have a cassette 
marker. But if it broke the handy dandy pencil would be our sure known 
tool of choice. 



Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



Sherwood, Margaret  msherw...@partners.org 
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/19/2010 01:49 PM

To
Nita Searcy nsea...@swmail.sw.org, histonet@lists.utsouthwestern.edu
cc

Subject
RE: [Histonet] Cassette Marking






Nita,

I would say you have quite a choice!  For the longest time, we had so much
trouble with so many pens, we went to the pencil for the cassettes which 
are the
best.  But we still needed pens for the slides; that's when we switched to
StatLab.  We still use the pencil for the cassettes.

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita 
Searcy
Sent: Tuesday, October 19, 2010 2:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking
Importance: High

** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens 
?
Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street 
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438



The information in this e-mail is intended only for the person to whom it 
is
addressed. If you believe this e-mail was sent to you in error and the 
e-mail
contains patient information, please contact the Partners Compliance 
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in 
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but does not contain patient information, please contact the sender and 
properly
dispose of the e-mail.


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This message along with any attached data, are the confidential and
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RE: [Histonet] Cassette Marking

2010-10-19 Thread hymclab
We use the pens from Statlab also and love them.

Dawn D. Schneider, HT(ASCP)
Lead Histology Tech
Howard Young Medical Center
240 Maple St.
Woodruff, WI  54568
715-356-8174


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, 
Margaret
Sent: Tuesday, October 19, 2010 1:38 PM
To: Nita Searcy; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cassette Marking

The marking pencils work fine and we just ordered some marking pens from 
StatLab which work great.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Tuesday, October 19, 2010 2:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking
Importance: High

** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens ?
Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


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[Histonet] Autofluorescence and literature for getting rid of the problem

2010-10-19 Thread gayle callis
Richard, 

 

You will find what you need in a free pdf on autofluorescence from Wright
Imaging Facility in Toronto Canada.  They have a website, then download
Autofluorescence:  Causes and Cures.  Also, if you can't get rid of
autofluorescence , use a near infrared fluorophore e.g. Alexa 750.  There is
no autofluorescence seen in the NIR range.  Just Google the title and it
will come up instantly.  They also have a pdf on Mounting Media for
fluorescence work.

 

 

For the little FFPE fluorescent work we do, you can try 100 to 300 mM
glycine in TRIS buffer pH 7.4 for 20 to 30 minutes before embarking on
immunostaining.   Glycine binds free aldehydes to reduce the
autofluorescence but doesn't always work 100%.  It may reduce the problem
but not totally eliminate it.  

 

There are other references on getting rid of autofluorescence, one for GFP,
a review, but it applies to FFPE tissue even not containing GFP.   I will be
happy to  send the pdf if you wish.   

 

Gayle M. Callis

HTL/HT/MT(ASCP) 

Bozeman MT 

 

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Re: [Histonet] Cassette Marking

2010-10-19 Thread Sean McBride
Nita,

We use HistoTec pens by Newcomer Supply

~Sean


On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote:

 ** High Priority **
 
 If you HAVE to manually mark cassettes - what are you using? Cassette pens ? 
 Pencils ? What is the rest of the world doing?
 
 Anything else on the market?
 
 Thanks
 
 
 
 Nita Searcy, HT/HTL (ASCP)
 Scott and White Hospital
 Division Manager, Anatomic Pathology
 2401 S. 31st. Street 
 254-724-2438
 Temple, Texas, 76502
 nsea...@swmail.sw.org
 
 
 254-724-2438
 
 Nita Searcy.vcf___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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Re: [Histonet] Cassette Marking

2010-10-19 Thread Victor Tobias
 I'm somewhat surprised that many labs are still handwriting blocks and 
slides. If you are using a LIS, can it integrate printing blocks and 
slides? Is the cost too high to add the printing capability? The cost of 
equipment is so cheap compared with one lawsuit.


It would also reduce the stress of loosing your job over a labeling 
mistake. Just seems like a win win for everyone.


Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.


On 10/19/2010 1:38 PM, Sean McBride wrote:

Nita,

We use HistoTec pens by Newcomer Supply

~Sean


On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote:


** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens ? 
Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438

Nita Searcy.vcf___
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RE: [Histonet] Cassette Marking

2010-10-19 Thread sgoebel
As compared to a lawsuit, yes it's cheaper.  But, when trying to explain
to a budget committee that you need something to label things that you
can do by hand...they usually don't see the point.  This brings me back
to my original point of mislabelling things 2 times in a year...

Sarah Goebel, B.A., HT (ASCP)

Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907




 Original Message 
Subject: Re: [Histonet] Cassette Marking
From: Victor Tobias vic...@pathology.washington.edu
Date: Tue, October 19, 2010 1:56 pm
To: histonet@lists.utsouthwestern.edu

 I'm somewhat surprised that many labs are still handwriting blocks and 
slides. If you are using a LIS, can it integrate printing blocks and 
slides? Is the cost too high to add the printing capability? The cost of

equipment is so cheap compared with one lawsuit.

It would also reduce the stress of loosing your job over a labeling 
mistake. Just seems like a win win for everyone.

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.


On 10/19/2010 1:38 PM, Sean McBride wrote:
 Nita,

 We use HistoTec pens by Newcomer Supply

 ~Sean


 On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote:

 ** High Priority **

 If you HAVE to manually mark cassettes - what are you using? Cassette pens ? 
 Pencils ? What is the rest of the world doing?

 Anything else on the market?

 Thanks



 Nita Searcy, HT/HTL (ASCP)
 Scott and White Hospital
 Division Manager, Anatomic Pathology
 2401 S. 31st. Street
 254-724-2438
 Temple, Texas, 76502
 nsea...@swmail.sw.org


 254-724-2438

 Nita Searcy.vcf___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 ___
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RE: [Histonet] Cassette Marking

2010-10-19 Thread Vanessa Avalos
Most places will also send you a sample. This way you can run test blocks in
your processor to make sure you are happy w/ the results. Just remember to
label the block w/ the pen name so you  wont get confused! :) I currently
use Mercedes Platinum Marker

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jacqueline
Farnsworth
Sent: Tuesday, October 19, 2010 11:43 AM
To: Sherwood, Margaret ; Nita Searcy; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cassette Marking

Surgipath  01880
Permanent Ink . Ultra fine point. Non-toxic

Jacqueline Farnsworth
Anatomic Pathology, Tech III
Diagnostic Scientific Centre
Calgary Laboratory Services

Phone: 403-770-3588
Pager: 403-212-8223  X07630

P Please consider the environment before printing this email.

From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret
[msherw...@partners.org]
Sent: October 19, 2010 12:38 PM
To: Nita Searcy; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cassette Marking

The marking pencils work fine and we just ordered some marking pens from
StatLab
which work great.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Tuesday, October 19, 2010 2:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking
Importance: High

** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens ?
Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
contains patient information, please contact the Partners Compliance
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in
error
but does not contain patient information, please contact the sender and
properly
dispose of the e-mail.


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RE: [Histonet] Cassette Marking

2010-10-19 Thread Sherwood, Margaret
Victor,

We are a research lab and don't generate near the amount of specimens that a
clinical lab would.  We were happy to get an automatic stainer and coverslipper!

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias
Sent: Tuesday, October 19, 2010 4:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cassette Marking


  I'm somewhat surprised that many labs are still handwriting blocks and 
slides. If you are using a LIS, can it integrate printing blocks and 
slides? Is the cost too high to add the printing capability? The cost of 
equipment is so cheap compared with one lawsuit.

It would also reduce the stress of loosing your job over a labeling 
mistake. Just seems like a win win for everyone.

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.


On 10/19/2010 1:38 PM, Sean McBride wrote:
 Nita,

 We use HistoTec pens by Newcomer Supply

 ~Sean


 On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote:

 ** High Priority **

 If you HAVE to manually mark cassettes - what are you using? Cassette pens ?
Pencils ? What is the rest of the world doing?

 Anything else on the market?

 Thanks



 Nita Searcy, HT/HTL (ASCP)
 Scott and White Hospital
 Division Manager, Anatomic Pathology
 2401 S. 31st. Street
 254-724-2438
 Temple, Texas, 76502
 nsea...@swmail.sw.org


 254-724-2438

 Nita Searcy.vcf___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 ___
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Re: [Histonet] Cassette Marking

2010-10-19 Thread histot...@imagesbyhopper.com
Statlab pens for us.  We have a cassette printer, but we hand write our slides. 
 I *love* the statlab pens!!

Sent from my iPod

On Oct 19, 2010, at 5:26 PM, Sherwood, Margaret  msherw...@partners.org 
wrote:

 Victor,
 
 We are a research lab and don't generate near the amount of specimens that a
 clinical lab would.  We were happy to get an automatic stainer and 
 coverslipper!
 
 Peggy 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias
 Sent: Tuesday, October 19, 2010 4:57 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Cassette Marking
 
 
  I'm somewhat surprised that many labs are still handwriting blocks and 
 slides. If you are using a LIS, can it integrate printing blocks and 
 slides? Is the cost too high to add the printing capability? The cost of 
 equipment is so cheap compared with one lawsuit.
 
 It would also reduce the stress of loosing your job over a labeling 
 mistake. Just seems like a win win for everyone.
 
 Victor
 
 Victor Tobias
 Clinical Applications Analyst
 University of Washington Medical Center
 Dept of Pathology Room BB220
 1959 NE Pacific
 Seattle, WA 98195
 vic...@pathology.washington.edu
 206-598-2792
 206-598-7659 Fax
 =
 Privileged, confidential or patient identifiable information may be
 contained in this message. This information is meant only for the use
 of the intended recipients. If you are not the intended recipient, or
 if the message has been addressed to you in error, do not read,
 disclose, reproduce, distribute, disseminate or otherwise use this
 transmission. Instead, please notify the sender by reply e-mail, and
 then destroy all copies of the message and any attachments.
 
 
 On 10/19/2010 1:38 PM, Sean McBride wrote:
 Nita,
 
 We use HistoTec pens by Newcomer Supply
 
 ~Sean
 
 
 On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote:
 
 ** High Priority **
 
 If you HAVE to manually mark cassettes - what are you using? Cassette pens ?
 Pencils ? What is the rest of the world doing?
 
 Anything else on the market?
 
 Thanks
 
 
 
 Nita Searcy, HT/HTL (ASCP)
 Scott and White Hospital
 Division Manager, Anatomic Pathology
 2401 S. 31st. Street
 254-724-2438
 Temple, Texas, 76502
 nsea...@swmail.sw.org
 
 
 254-724-2438
 
 Nita Searcy.vcf___
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[Histonet] Coverslipping video

2010-10-19 Thread Caroline Bass
Hello,

I¹m wondering if anyone could direct me to an online video of coverslipping,
preferably permount. I¹d like a reference to show some newbies in the lab.

Thanks,

Caroline
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