[Histonet] RE: Plain Vanilla Autostainer?

2010-10-20 Thread Susan.Walzer
Leica Auto Stainer is the only way to go. It does all that you ask for and ours 
is old and still a work horse.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin
Sent: Tuesday, October 19, 2010 2:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Plain Vanilla Autostainer?

I am a simple man in a simple lab with simple needs.  I work in a multiple-lab 
research environment, typically providing paraffin/frozen sections + HE to 
individuals who then perform their own manual IHC.  A simple man, yes, but also 
a busy man, who needs to be freed from staining dishes in order to pursue other 
tasks.

What we need: (1) Deparaffinization only; (2) Deparaffinization -- HE; (3) 
HE only system, preferably with (4) a relatively small, preferably benchtop 
footprint (current unit lives in a 30 X 33 X 34 vented workstation).  
Automatic coverslipping extremely optional.

What we have: Thermo Shandon Varistain 24-4, which performed these mundane 
tasks admirably until going electronically insane.  It's been denied a service 
contract, the production line has been discontinued and the writing is on the 
wall.

What seem to be available:  Special staining systems with a whole lot of bells 
and whistles, designed primarily for a high-throughput hospital setting.

Is there anything out there that would serve my needs, even if it is a fancy 
system that would be slumming it here?  Or is mine just too obsolete a market?  
Price up to $15K, slightly negotiable.  (Bench space only slightly negotiable; 
floor model if I must.)

Many thanks,

Kevin Johnson
University of Miami
Diabetes Research Institute
Miami, FL
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RE: [Histonet] Cassette Marking

2010-10-20 Thread Nita Searcy
As I sent to Victor- only mission critical  items are being bought and as 
yet, I have not proved mission critical!
One can;'t show errors not caught until way after the fact!

Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street 
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438

 sgoe...@xbiotech.com 10/19/2010 4:04 PM 
As compared to a lawsuit, yes it's cheaper.  But, when trying to explain
to a budget committee that you need something to label things that you
can do by hand...they usually don't see the point.  This brings me back
to my original point of mislabelling things 2 times in a year...

Sarah Goebel, B.A., HT (ASCP)

Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907




 Original Message 
Subject: Re: [Histonet] Cassette Marking
From: Victor Tobias vic...@pathology.washington.edu
Date: Tue, October 19, 2010 1:56 pm
To: histonet@lists.utsouthwestern.edu 

 I'm somewhat surprised that many labs are still handwriting blocks and 
slides. If you are using a LIS, can it integrate printing blocks and 
slides? Is the cost too high to add the printing capability? The cost of

equipment is so cheap compared with one lawsuit.

It would also reduce the stress of loosing your job over a labeling 
mistake. Just seems like a win win for everyone.

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu 
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
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On 10/19/2010 1:38 PM, Sean McBride wrote:
 Nita,

 We use HistoTec pens by Newcomer Supply

 ~Sean


 On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote:

 ** High Priority **

 If you HAVE to manually mark cassettes - what are you using? Cassette pens ? 
 Pencils ? What is the rest of the world doing?

 Anything else on the market?

 Thanks



 Nita Searcy, HT/HTL (ASCP)
 Scott and White Hospital
 Division Manager, Anatomic Pathology
 2401 S. 31st. Street
 254-724-2438
 Temple, Texas, 76502
 nsea...@swmail.sw.org 


 254-724-2438

 Nita Searcy.vcf___
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BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Nita Searcy
TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org
N:Searcy;Nita
TITLE:Manager, Pathology Division
TEL;PAGER:633-2370
END:VCARD

BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Nita Searcy
TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org
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TITLE:Manager, Pathology Division
TEL;PAGER:633-2370
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[Histonet] m.bovis

2010-10-20 Thread Kathleen Jones
Hello Histonet! 

I know this has come up in the past, but I have not been able to find a
solution in the archives...I am trying to get a mycoplasma bovis
protocol worked up and am having tremendous difficulties. My antibody
strain is M23 but it does not seem to be working very well for my IHC
applications. I have tried several antigen retrieval methods, antibody
dilutions and detection methods to no avail. I am thinking I need a new
source of antibody...Can anyone suggest a commercially available m.bovis
antibody that works for them? Any suggestions at all would be greatly
appreciated!

Thanks!!
Kathleen


Kathleen Jones
Research Technologist
Pathology/Microbiology
AVC - UPEI
(902)566-0595


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[Histonet] RE: Plain Vanilla Autostainer?

2010-10-20 Thread Cheri Miller
I will second that!

Cheryl A. Miller HT(ASAP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Wednesday, October 20, 2010 2:19 AM
To: kjohn...@med.miami.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Plain Vanilla Autostainer?

Leica Auto Stainer is the only way to go. It does all that you ask for and ours 
is old and still a work horse.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin
Sent: Tuesday, October 19, 2010 2:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Plain Vanilla Autostainer?

I am a simple man in a simple lab with simple needs.  I work in a multiple-lab 
research environment, typically providing paraffin/frozen sections + HE to 
individuals who then perform their own manual IHC.  A simple man, yes, but also 
a busy man, who needs to be freed from staining dishes in order to pursue other 
tasks.

What we need: (1) Deparaffinization only; (2) Deparaffinization -- HE; (3) 
HE only system, preferably with (4) a relatively small, preferably benchtop 
footprint (current unit lives in a 30 X 33 X 34 vented workstation).  
Automatic coverslipping extremely optional.

What we have: Thermo Shandon Varistain 24-4, which performed these mundane 
tasks admirably until going electronically insane.  It's been denied a service 
contract, the production line has been discontinued and the writing is on the 
wall.

What seem to be available:  Special staining systems with a whole lot of bells 
and whistles, designed primarily for a high-throughput hospital setting.

Is there anything out there that would serve my needs, even if it is a fancy 
system that would be slumming it here?  Or is mine just too obsolete a market?  
Price up to $15K, slightly negotiable.  (Bench space only slightly negotiable; 
floor model if I must.)

Many thanks,

Kevin Johnson
University of Miami
Diabetes Research Institute
Miami, FL
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[Histonet] TN - HT Opprotunity

2010-10-20 Thread Hale, Meredith
Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mh...@carisls.com

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mh...@carisls.com 

 

 

 

 

 

 

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RE: [Histonet] RE: Plain Vanilla Autostainer?

2010-10-20 Thread Sherwood, Margaret
We also love our Leica Autostainer XL (refurbished).  Kevin, that is the way to
go if money is tight. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Wednesday, October 20, 2010 8:45 AM
To: susan.wal...@hcahealthcare.com; kjohn...@med.miami.edu;
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Plain Vanilla Autostainer?

I will second that!

Cheryl A. Miller HT(ASAP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
susan.wal...@hcahealthcare.com
Sent: Wednesday, October 20, 2010 2:19 AM
To: kjohn...@med.miami.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Plain Vanilla Autostainer?

Leica Auto Stainer is the only way to go. It does all that you ask for and ours
is old and still a work horse.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin
Sent: Tuesday, October 19, 2010 2:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Plain Vanilla Autostainer?

I am a simple man in a simple lab with simple needs.  I work in a multiple-lab
research environment, typically providing paraffin/frozen sections + HE to
individuals who then perform their own manual IHC.  A simple man, yes, but also
a busy man, who needs to be freed from staining dishes in order to pursue other
tasks.

What we need: (1) Deparaffinization only; (2) Deparaffinization -- HE; (3) HE
only system, preferably with (4) a relatively small, preferably benchtop
footprint (current unit lives in a 30 X 33 X 34 vented workstation).
Automatic coverslipping extremely optional.

What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks
admirably until going electronically insane.  It's been denied a service
contract, the production line has been discontinued and the writing is on the
wall.

What seem to be available:  Special staining systems with a whole lot of bells
and whistles, designed primarily for a high-throughput hospital setting.

Is there anything out there that would serve my needs, even if it is a fancy
system that would be slumming it here?  Or is mine just too obsolete a market?
Price up to $15K, slightly negotiable.  (Bench space only slightly negotiable;
floor model if I must.)

Many thanks,

Kevin Johnson
University of Miami
Diabetes Research Institute
Miami, FL
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RE: [Histonet] RE: Plain Vanilla Autostainer?

2010-10-20 Thread Bernice Frederick
We use the Autostainer XL as well. Just got a new one with the attached
coverslipper. The old one is now in IHC for deparaffinization and running
down before coverslipping. We have a program on the new autostainer that
picks up from the load drawer and goes to the last xylene for the
coverslipper to do its thing!
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Wednesday, October 20, 2010 7:45 AM
To: susan.wal...@hcahealthcare.com; kjohn...@med.miami.edu;
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Plain Vanilla Autostainer?

I will second that!

Cheryl A. Miller HT(ASAP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
susan.wal...@hcahealthcare.com
Sent: Wednesday, October 20, 2010 2:19 AM
To: kjohn...@med.miami.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Plain Vanilla Autostainer?

Leica Auto Stainer is the only way to go. It does all that you ask for and
ours is old and still a work horse.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson,
Kevin
Sent: Tuesday, October 19, 2010 2:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Plain Vanilla Autostainer?

I am a simple man in a simple lab with simple needs.  I work in a
multiple-lab research environment, typically providing paraffin/frozen
sections + HE to individuals who then perform their own manual IHC.  A
simple man, yes, but also a busy man, who needs to be freed from staining
dishes in order to pursue other tasks.

What we need: (1) Deparaffinization only; (2) Deparaffinization -- HE; (3)
HE only system, preferably with (4) a relatively small, preferably benchtop
footprint (current unit lives in a 30 X 33 X 34 vented workstation).
Automatic coverslipping extremely optional.

What we have: Thermo Shandon Varistain 24-4, which performed these mundane
tasks admirably until going electronically insane.  It's been denied a
service contract, the production line has been discontinued and the writing
is on the wall.

What seem to be available:  Special staining systems with a whole lot of
bells and whistles, designed primarily for a high-throughput hospital
setting.

Is there anything out there that would serve my needs, even if it is a fancy
system that would be slumming it here?  Or is mine just too obsolete a
market?  Price up to $15K, slightly negotiable.  (Bench space only slightly
negotiable; floor model if I must.)

Many thanks,

Kevin Johnson
University of Miami
Diabetes Research Institute
Miami, FL
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[Histonet] Laser Capture Machine

2010-10-20 Thread Reuel Cornelia
To anybody using a laser capture machine, I want to ask your opinion on the 
following machine which one is better with regard to their instrumentation and 
cost of consumables because we are in the process of purchasing one and your 
honest opinion with regards to handling this machine is very much accountable. 
1. Arcturus XT
2. Leica AS LMD
3. P.A.L.M LPC
4. MMI Cell Cut
5. Arcturus PixCell lle

Thank you very much.



Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768



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[Histonet] Histotech needed can you help?

2010-10-20 Thread Pam Barker
Hi Histonetters!!
I hope you are enjoying a beautiful Fall Day.  

.  I am currently working with a hospital in Northern Indiana about an
hour south of Chicago that is in need of a histo tech.  This is a
permanent full time dayshift position.  The client offers a great
environment, a great crew to work with and excellent salary, benefits
and relocation assistance.

 

My question is do you know of anyone who might be interested in this
position?

 

If you think you or someone you know might be interested please contact
me.

I can be reached at 866-607-3542 or  mailto:rel...@earthlink.net
rel...@earthlink.net  Thanks-Pam

 
 
Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail:  mailto:rel...@earthlink.net rel...@earthlink.net 
 http://www.facebook.com www.facebook.com  search Pam Barker RELIA
 http://www.linkedin.com/reliasolutions
www.linkedin.com/reliasolutions
 http://www.myspace.com/pamatrelia www.myspace.com/pamatrelia
 http://www.twitter.com/pamatrelia www.twitter.com/pamatrelia 

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[Histonet] Job Opening in West Columbia, SC

2010-10-20 Thread Blake Taylor
Hello everyone, we have an open position here at Lexington Medical Center in 
West Columbia, South Carolina for a full-time histotech. If you are interested 
or know anyone who may be please contact me or apply through our website.  
www.lexmed.com

thanks 

Blake Taylor
Section Supervisor
Dept. of Pathology
Lexington Medical Center
803-936-8214
bcdu...@lexhealth.org


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[Histonet] Job Opening in San Diego

2010-10-20 Thread Alyssa Peterson
Allied Search Partners is currently accepting resumes for a
histotechnician/histotechnologist in San Diego, CA



*Shift:* Full Time, Day Shift, Monday-Friday



*Environment:*

New State of the art Laboratory. Flexible hours. Ability to work side by
side with Pathologist. No quotas. Family oriented environment.



*Location:*

San Diego, CA



*Essential Functions and Duties:*



Must be able to work independently under minimal supervision, maintain
laboratory supplies, equipment, and QC/QA records



The person should be reliable with great inter-personal communication
skills, and willing to coordinate with Pathologist



*Requirements:*

Must meet the CLIA standards to gross**

HT (ASCP) preferred.



To apply for this position please submit resume to
aly...@alliedsearchpartners.com for initial prescreening. No resume will be
submitted to client until we speak to you for a phone interview. All resumes
kept confidential.


-- 
Alyssa Peterson, Director of Candidate Recruitment
LinkedIN:http://www.linkedin.com/in/alyssapetersonasp

Allied Search Partners

T: 888.388.7571

F: 888.388.7572

www.alliedsearchpartners.com

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[Histonet] IEC-CTD IEC Minotome Cryostat Wanted!!!

2010-10-20 Thread Eric Baltazar
Greetings to you all fellow Histonetters!

Our company is currently looking to buy any available *Cryostat model
IEC-CTD  Minotome *for use in our mobile Mohs laboratoy service
business. If you still have these units in your offices and storages or
probably know somebody who have this equipment and have not use it for years
bec. they now have a much modern unit to use, contact us *ASAP* through this
email add or on the phone listed below so we can coordinate purchasing and
shipping procedure. We will take care of the shipping cost on these
machines.

This *Cryostat IEC-CTD* are the ones that looks like a mini-ref w/ caster
wheels produced in the 70's,the Minotome would be the updated model w/ wider
body and both are use to cut frozen sections. These are vintage machines but
we can surely utilize them for our mobile Mohs lab business.

TURN THAT SLEEPING MACHINE INTO *GREEN BUCKS* AND *WE WILL BUY THESE UNITS
REGARDLESS OF THEIR CONDITIONS*  So act and call us now!

Sincerely,

Eric T. Baltazar
Dermtech Mohs Services Group Inc.
Tel.no.:707-651-9420
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[Histonet] RE: Cassette Marking

2010-10-20 Thread Adam Harris
Hi All,

If anyone would like to try a free sample of the KP Lab marker for
cassette, slide, or general purpose labeling, feel free to contact me and I
will be more than happy to send you one. It's a win-win situation!! 

Adam Harris
Sales Associate 
Sensor Health Inc. 
110-6 Turnbull Crt.
Cambridge, ON
N1T 1K6
T: 1-888-777-7080
T: 519-621-1515
F: 519-621-8778
www.sensorhealth.com

-Original Message-
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[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: None
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 83, Issue 33

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Today's Topics:

   1. RE: Cassette Marking (hymclab)
   2. Autofluorescence and literature for getting rid of the
  problem  (gayle callis)
   3. Re: Cassette Marking (Sean McBride)
   4. Re: Cassette Marking (Victor Tobias)
   5. RE: Cassette Marking (sgoe...@xbiotech.com)
   6. RE: Cassette Marking (Vanessa Avalos)
   7. RE: Autofluorescence and literature for getting rid ofthe
  problem (WILLIAM DESALVO)
   8. RE: Cassette Marking (Sherwood, Margaret )
   9. Re: Cassette Marking (histot...@imagesbyhopper.com)
  10. Coverslipping video (Caroline Bass)
  11. RE: Plain Vanilla Autostainer? (susan.wal...@hcahealthcare.com)
  12. RE: Cassette Marking (Nita Searcy)
  13. m.bovis (Kathleen Jones)
  14. RE: Plain Vanilla Autostainer? (Cheri Miller)
  15. TN  -   HT Opprotunity  (Hale, Meredith)
  16. RE: RE: Plain Vanilla Autostainer? (Sherwood, Margaret )
  17. RE: RE: Plain Vanilla Autostainer? (Bernice Frederick)
  18. Laser Capture Machine (Reuel Cornelia)
  19. Histotech needed can you help? (Pam Barker)
  20. Job Opening in San Diego (Alyssa Peterson)


--

Message: 1
Date: Tue, 19 Oct 2010 14:49:18 -0500
From: hymclab hymclab.hymc...@ministryhealth.org
Subject: RE: [Histonet] Cassette Marking
To: 'Sherwood, Margaret ' msherw...@partners.org, Nita Searcy
nsea...@swmail.sw.org, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:

ef3001233998854390c692d19b058f0f6d8a650...@exmhcmbx01vs.ministryhealth.net

Content-Type: text/plain; charset=us-ascii

We use the pens from Statlab also and love them.

Dawn D. Schneider, HT(ASCP)
Lead Histology Tech
Howard Young Medical Center
240 Maple St.
Woodruff, WI  54568
715-356-8174


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood,
Margaret
Sent: Tuesday, October 19, 2010 1:38 PM
To: Nita Searcy; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cassette Marking

The marking pencils work fine and we just ordered some marking pens from
StatLab which work great.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Tuesday, October 19, 2010 2:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Marking
Importance: High

** High Priority **

If you HAVE to manually mark cassettes - what are you using? Cassette pens ?
Pencils ? What is the rest of the world doing?

Anything else on the market?

Thanks



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
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HelpLine at
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it or its contents 

[Histonet] Sara Phinney-availability

2010-10-20 Thread Sara . Phinney

I will be out of the office starting  10/20/2010 and will not return until
10/22/2010.

I will be in the office but with limited availability from October18th till
Friday October 22.
I will be checking voicemail and email periodically throughout the day.

My best,
Sara

Please contact Mark Burton in my absence:
 mark.bur...@leica-microsystems.com


If you require technical assistance please dial 1-800-248-0123.

For all other matters please call Timm Piper at 617-653-2605.


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[Histonet] IHC techs needed

2010-10-20 Thread Troutman, Kenneth A
Vanderbilt University is seeking two certified techs for 2nd shift.  Primary 
duties will be IHC staining.  Anyone interested please visit 
www.mc.vanderbilt.eduhttp://www.mc.vanderbilt.edu/ and click on Careers at 
Vanderbilt.  Select Current staff openings and type Histotech in the 
keyword search.  Thank you!

Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN  37232
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[Histonet] Celestine Blue for Technovit 7200

2010-10-20 Thread Suzanne Bruce
Hi everyone,
 
I'm searching for a procedure or help w/a stain for thick ground sections using 
Technovit 7200.  The stain in question is a celestine blue/alum hematoxylin w/a 
Van Gieson counterstain.  Anyone have a procedure or recommendations?
 
Thanks in advance,
Suzanne
sbr...@vetpathservicesinc.com
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[Histonet] Human anti human

2010-10-20 Thread sgoebel
Hey ya'll
I was going through the archives to try and find a solution to this, but
the last post was in 2008, so I thought I would throw it back out there.
 I need a human anti human polymer...is there one yet? I looked and
couldn't find one through my normal places.
As to not start the conversation that is on the archive up again. I
have tried several approaches already to no avail. I tried FITC, I
conjugated the primary human anti human with HRP, I've tried TSA (and
tons of other companies version of TSA)...no luck...
My antibody just isn't staining...I even tried putting it on the tissue
and PBMCs at a 1:1 dilution...nope...any thoughts out there? I want to
rip my hair out 


Sarah Goebel, B.A., HT (ASCP)

Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907


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RE: [Histonet] Celestine Blue for Technovit 7200

2010-10-20 Thread Jack Ratliff

Why not use Sanderson's Rapid Bone Stain with Van Gieson picrofuchsin? You can 
get the Sanderson's and one or all of three counterstains for this particular 
kind of staining in a kit from Dorn and Hart Microedge (www.dornandhart.com).
 
Jack
 

 
 Date: Wed, 20 Oct 2010 16:52:26 -0400
 From: sbr...@vetpathservicesinc.com
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Celestine Blue for Technovit 7200
 
 Hi everyone,
 
 I'm searching for a procedure or help w/a stain for thick ground sections 
 using Technovit 7200. The stain in question is a celestine blue/alum 
 hematoxylin w/a Van Gieson counterstain. Anyone have a procedure or 
 recommendations?
 
 Thanks in advance,
 Suzanne
 sbr...@vetpathservicesinc.com
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[Histonet] QMS MANUAL

2010-10-20 Thread Adrienne Aperghis Kavanagh

Does anyone have a basic format or table of contents they would be willing to 
share regarding a QMS (Quality Management Systems) Manual?
I am in the process of getting one together and am a little overwhelmed.  We 
are supposed to base our QMS on NYSDOH...as if that helps.  Even a link in the 
right direction would be MUCH appreciated.  You can message me privately, if 
you wish.

Thanks in advance, friends!

ADRIENNE

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RE: [Histonet] Celestine Blue for Technovit 7200

2010-10-20 Thread Suzanne Bruce
Thanks Jack, what's the difference between the celestine blue w/alum 
hematoxylin and the Sanderson's?  What's the difference between the Sanderson's 
and the Masson's Goldner's?  Have you tried the celestine blue stain?
 
Thanks in advance, 
Suzanne
 


From: Jack Ratliff [mailto:ratliffj...@hotmail.com]
Sent: Wed 10/20/2010 5:04 PM
To: Suzanne Bruce; Histonet
Subject: RE: [Histonet] Celestine Blue for Technovit 7200


Why not use Sanderson's Rapid Bone Stain with Van Gieson picrofuchsin? You can 
get the Sanderson's and one or all of three counterstains for this particular 
kind of staining in a kit from Dorn and Hart Microedge (www.dornandhart.com 
http://www.dornandhart.com/ ).
 
Jack
 

 
 Date: Wed, 20 Oct 2010 16:52:26 -0400
 From: sbr...@vetpathservicesinc.com
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Celestine Blue for Technovit 7200
 
 Hi everyone,
 
 I'm searching for a procedure or help w/a stain for thick ground sections 
 using Technovit 7200. The stain in question is a celestine blue/alum 
 hematoxylin w/a Van Gieson counterstain. Anyone have a procedure or 
 recommendations?
 
 Thanks in advance,
 Suzanne
 sbr...@vetpathservicesinc.com
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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