RE: [Histonet] unstained paraffin tissue slides storage
Room temperature storage has always worked fine for us, of course it all depends on the quality of fixation and processing, and what antigen you are after.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: 03 November 2010 20:55 To: sgoe...@xbiotech.com; Pop Elena Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage Hello, We have been storing our slides in very small Ziploc bags at -20 and find that this method works fairly well. We have done a study(Berez et.al in process) and slides stored in this fashion for 5 years stain better than freshly cut slides from the same blocks that have been stored at room temperature. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.com Sent: Wednesday, November 03, 2010 4:26 PM To: Pop Elena Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage Plain slide boxes is ok. I think you can store them for up to a year in a fridg. (4 degrees), but I usually pu them in a freezer (-20). Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] unstained paraffin tissue slides storage From: Pop Elena med_l...@yahoo.com Date: Wed, November 03, 2010 1:21 pm To: histonet@lists.utsouthwestern.edu Hello, I found here a few disscussions regarding the storage of tissue slides but I did not find a clear answer to the questions I have. I would really appreciate an answer from anybody that has experience with this. I need to store for long term a bunch of unstained tissue slides for the purpose of doing immunostaining even in a few years from now on. Unfortunatelly they were stored for about 3 years at room temperature. What it is usually recomended: to store them at -20 degrees Celsius? If yes, is it OK to store them in the regular 100 slides boxes? And when you need to start an immunostaining just take them out of the freezer and let them at room temp for a while before starting the stain or what procedure do you use? I heard some labs keep them in nitrogen gas containers. Do you have any info about this? Any imput is appreciated! Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] eosin as tissue dye
I know using eosin as a tissue dye during processing has been a recent discussion. My lab has been experiencing issue with the cobalt blue we use currently. At embedding, small biopsies are not seen on blue sponges, white sponges turn blue. Biopsies that are wrapped are not turning blue, the paper is not allowing the blue thru. Eosin has come up as a suggestion. I know it is known to fluoresce. What is everyone's experience with this?? The only FISH test we are running is Her2 by pathvysion. What recipe are you all using?? On the processor?? Thank you so much. Melissa The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] unstained paraffin tissue slides storage--why cold?
-70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay viable. I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours talulahg...@gmail.com Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] unstained paraffin tissue slides storage--why cold?
The blocks also degrade but just on the surface, where the air oxygen can act. When you use the block for recuts to be used in IHC you always have to resurface the block, hence eliminating the oxidized surface layer and exposing the preserved deeper layer that you are actually using in the test. René J. --- On Thu, 11/4/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote: From: sgoe...@xbiotech.com sgoe...@xbiotech.com Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? To: Emily Sours talulahg...@gmail.com Cc: histonet@lists.utsouthwestern.edu Date: Thursday, November 4, 2010, 11:21 AM -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay viable. I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours talulahg...@gmail.com Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] unstained paraffin tissue slides storage--why cold?
The study that we did showed that the staining on freshly cut slides from a block stored at room temperature was in fact not as good as slides that were sectioned 5 years earlier and stored at -20. Therefore the blocks should be stored in the cold as well. The tissue is degrading in the block and on the slides and the cold does slow down the process. The fixation in lots of the tissue is not optimal. Fixing for 48 hours will definitely be better than the current fixation practices going on in most clinical labs, if what you want is the best tissue preservation possible. But most of us do not have that option. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.com Sent: Thursday, November 04, 2010 11:21 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay viable. I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours talulahg...@gmail.com Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] unstained paraffin tissue slides storage--why cold?
Helen, did you write a paper from that study? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, November 04, 2010 8:56 AM To: sgoe...@xbiotech.com; Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? The study that we did showed that the staining on freshly cut slides from a block stored at room temperature was in fact not as good as slides that were sectioned 5 years earlier and stored at -20. Therefore the blocks should be stored in the cold as well. The tissue is degrading in the block and on the slides and the cold does slow down the process. The fixation in lots of the tissue is not optimal. Fixing for 48 hours will definitely be better than the current fixation practices going on in most clinical labs, if what you want is the best tissue preservation possible. But most of us do not have that option. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.com Sent: Thursday, November 04, 2010 11:21 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay viable. I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours talulahg...@gmail.com Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Qualifications to run a Thin Prep 2000?
Dear Histonetters, I am interested in someone from the Cytyc Corp or a Cytology lab supervisor contacting me regarding the educational/training requirements for personnel running a Thin Prep 2000 instrument. I am on assignment at a Histology lab in Illinois where they process their own cytology specimens and they have a position open for someone to accession specimens and run the Thin Prep 2000. The Laboratory Director is of the opinion that they need to hire an HT or HTL to run this instrument, and I'm not so sure. I know I've worked in labs where they had lab assistants (college kids @ $8./hr) preparing thin preps. If the respondent could cite the relevant CAP regulation regarding the Thin Prep instrument, it would be fantastic. Thank you so much. Lee Luna Lives! Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] unstained paraffin tissue slides storage--why cold?
I did an experiment about 10 or 12 years ago, where I cut the sections and stained them at various intervals: Day1, Day 15, 1 month, 3 months, 6 months, 1 year. Before staining: Set of slides were allowed to sit at room temp (RT). Set of slides were stored at 4C. Set of slides were vacuum sealed and stored at RT. Set of slides were vacuum sealed and stored at 4C. Slides stored at 4C consistently stained well, with a slight variance in staining after 1 year. Did not matter weather they were vacuum sealed or not. Slides left out at RT did not fare so well. Staining variability was noticed after 1 month. When the slides were stained after one year, signal was almost eliminated. Variability and loss of antigenicity was observed with the vacuum sealed slides as well. I did this experiment for just one antibody which was being extensively used at the time for one of our projects. It could be that other antibodies fare mach better under RT conditions, but why take a chance? We keep all of our control slides at 4C. Blocks are kept at RT. Thanks Dusko Trajkovic From: Morken, Tim timothy.mor...@ucsfmedctr.org To: Helen Fedor hfe...@jhmi.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thu, November 4, 2010 9:09:16 AM Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? Helen, did you write a paper from that study? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, November 04, 2010 8:56 AM To: sgoe...@xbiotech.com; Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? The study that we did showed that the staining on freshly cut slides from a block stored at room temperature was in fact not as good as slides that were sectioned 5 years earlier and stored at -20. Therefore the blocks should be stored in the cold as well. The tissue is degrading in the block and on the slides and the cold does slow down the process. The fixation in lots of the tissue is not optimal. Fixing for 48 hours will definitely be better than the current fixation practices going on in most clinical labs, if what you want is the best tissue preservation possible. But most of us do not have that option. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.com Sent: Thursday, November 04, 2010 11:21 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay viable. I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours talulahg...@gmail.com Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] unstained paraffin tissue slides storage--why cold?
Hello, We have not published the paper yet . It is in progress and hope to get it out of the pile soon. Helen From: dusko trajkovic [mailto:dunat...@sbcglobal.net] Sent: Thursday, November 04, 2010 1:45 PM To: Morken, Tim; Helen Fedor Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? I did an experiment about 10 or 12 years ago, where I cut the sections and stained them at various intervals: Day1, Day 15, 1 month, 3 months, 6 months, 1 year. Before staining: Set of slides were allowed to sit at room temp (RT). Set of slides were stored at 4C. Set of slides were vacuum sealed and stored at RT. Set of slides were vacuum sealed and stored at 4C. Slides stored at 4C consistently stained well, with a slight variance in staining after 1 year. Did not matter weather they were vacuum sealed or not. Slides left out at RT did not fare so well. Staining variability was noticed after 1 month. When the slides were stained after one year, signal was almost eliminated. Variability and loss of antigenicity was observed with the vacuum sealed slides as well. I did this experiment for just one antibody which was being extensively used at the time for one of our projects. It could be that other antibodies fare mach better under RT conditions, but why take a chance? We keep all of our control slides at 4C. Blocks are kept at RT. Thanks Dusko Trajkovic From: Morken, Tim timothy.mor...@ucsfmedctr.org To: Helen Fedor hfe...@jhmi.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thu, November 4, 2010 9:09:16 AM Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? Helen, did you write a paper from that study? Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, November 04, 2010 8:56 AM To: sgoe...@xbiotech.commailto:sgoe...@xbiotech.com; Emily Sours Cc: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? The study that we did showed that the staining on freshly cut slides from a block stored at room temperature was in fact not as good as slides that were sectioned 5 years earlier and stored at -20. Therefore the blocks should be stored in the cold as well. The tissue is degrading in the block and on the slides and the cold does slow down the process. The fixation in lots of the tissue is not optimal. Fixing for 48 hours will definitely be better than the current fixation practices going on in most clinical labs, if what you want is the best tissue preservation possible. But most of us do not have that option. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 -Original Message- From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.commailto:sgoe...@xbiotech.com Sent: Thursday, November 04, 2010 11:21 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold? -70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay viable. I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours talulahg...@gmail.commailto:talulahg...@gmail.com Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept
[Histonet] Paraffin liver sections
Dear all, One of my colleagues has consulted me about some paraffin embedded mouse livers he's been trying to cut. They were fixed in neutral buffered formalin for 3 days and then processed and embedded into paraffin. He was trying to cut 5 uM sections on our microtome, except as soon as the blade hit the liver, the liver would start to roll up and collapse into a small sliver, and you'd just get sections of paraffin with a hole where the liver should be. We tried changing cutting angle and soaking the blocks in ice water with no success. This is the first time we've tried livers, but we routinely cut decalcified bones on that microtome and have never seen that. Any ideas on what is going on? Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
I know that I am opening the er/pr/her2 can of worms again but has anyone gone as far as to educate the breast surgeons of the fixation requirements or impose any type of cutoff for when they can submit their tissues. We do not work weekends and we do have an alternate processor with a weekend breast cycle, but we are still running into the issue of inadequately fixed breast tissue as the pathologists seem to be rushing the tissue through so we don't run over our allotted 48 hours. Also, we do not perform er/pr/her2 in house so we are unable to validate a longer fixation time. Any advice would be greatly appreciated. Thanks Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin liver sections
What is your processing schedule for the livers? It is hard to know without that information. Pam Marcum UAMS - Original Message - From: Adam . anonwu...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, November 4, 2010 1:08:03 PM Subject: [Histonet] Paraffin liver sections Dear all, One of my colleagues has consulted me about some paraffin embedded mouse livers he's been trying to cut. They were fixed in neutral buffered formalin for 3 days and then processed and embedded into paraffin. He was trying to cut 5 uM sections on our microtome, except as soon as the blade hit the liver, the liver would start to roll up and collapse into a small sliver, and you'd just get sections of paraffin with a hole where the liver should be. We tried changing cutting angle and soaking the blocks in ice water with no success. This is the first time we've tried livers, but we routinely cut decalcified bones on that microtome and have never seen that. Any ideas on what is going on? Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Original Message - From: Adam . anonwu...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, November 4, 2010 1:08:03 PM Subject: [Histonet] Paraffin liver sections Dear all, One of my colleagues has consulted me about some paraffin embedded mouse livers he's been trying to cut. They were fixed in neutral buffered formalin for 3 days and then processed and embedded into paraffin. He was trying to cut 5 uM sections on our microtome, except as soon as the blade hit the liver, the liver would start to roll up and collapse into a small sliver, and you'd just get sections of paraffin with a hole where the liver should be. We tried changing cutting angle and soaking the blocks in ice water with no success. This is the first time we've tried livers, but we routinely cut decalcified bones on that microtome and have never seen that. Any ideas on what is going on? Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Qualifications to run a Thin Prep 2000?
Jay, I hope you are referring to the Thin Prep 2000 as being so user-friendly that anyone can do it and not belittling laboratory assistants by your reference to monkeys and bananas. Everyone that works in the laboratory is an important part of the end result--patient care. Thank you, Amber M. Fimbres, MHA, CT(ASCP)HT This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Qualifications to run a Thin Prep 2000?
Of course that's what he meant. It is wonderful - even I can run it!! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fimbres, Amber Sent: Thursday, November 04, 2010 15:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Qualifications to run a Thin Prep 2000? Jay, I hope you are referring to the Thin Prep 2000 as being so user-friendly that anyone can do it and not belittling laboratory assistants by your reference to monkeys and bananas. Everyone that works in the laboratory is an important part of the end result--patient care. Thank you, Amber M. Fimbres, MHA, CT(ASCP)HT This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] alcoholic formalin
I have a client that uses alcoholic formalin as a primary fixative for small endometrial and cervical biopsies. What can I do to keep the cells from looking like raisins? We presently run these biopsies on a conventional processor with a short biopsy schedule. Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Qualifications to run a Thin Prep 2000?
Amber, I'm sorry if I offended anyone, I was merely referring to this instrument's wonderful ease of operation. Would you happen to know the answer to my question, as I see you are a cytotech? Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Qualifications to run a Thin Prep 2000?
Joyce, I was hoping that's what he meant too and wanted to clarify so it wouldn't be taken out of context. =) Amber -Original Message- From: Weems, Joyce [mailto:jwe...@sjha.org] Sent: Thursday, November 04, 2010 12:19 PM To: Fimbres, Amber; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Qualifications to run a Thin Prep 2000? Of course that's what he meant. It is wonderful - even I can run it!! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fimbres, Amber Sent: Thursday, November 04, 2010 15:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Qualifications to run a Thin Prep 2000? Jay, I hope you are referring to the Thin Prep 2000 as being so user-friendly that anyone can do it and not belittling laboratory assistants by your reference to monkeys and bananas. Everyone that works in the laboratory is an important part of the end result--patient care. Thank you, Amber M. Fimbres, MHA, CT(ASCP)HT This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: alcoholic formalin
Easy solution is to provide your client with 10% NBF Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Elmgren Sent: Thursday, November 04, 2010 3:24 PM To: Histonet Subject: [Histonet] alcoholic formalin I have a client that uses alcoholic formalin as a primary fixative for small endometrial and cervical biopsies. What can I do to keep the cells from looking like raisins? We presently run these biopsies on a conventional processor with a short biopsy schedule. Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin liver sections
Sounds to me like they are overprocessed (dried out). Try soaking them on ice for 30 minutes or so before cutting. Some people use a drop of fabric softener on their ice tray to soften the tissue, or there are commercial products like soft block from Polyscience. Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Qualifications to run a Thin Prep 2000?
Jay, Our cytology laboratory is a recent convert to the Thin Prep system so I may not be able to answer your question in all of its entirety. However, our Cytyc/Hologic representative trained everyone (cytotechs and lab assistants) on how to operate the Thin Prep instruments. Cytyc had a competency form which listed who was trained and when and was then signed by the Cytyc rep. This paper is on file in our department. As to if a Thin Prep trained cytotech at your place of employment can perform the same training in place of a Cytyc representative, I'm unsure. Our Cytyc rep comes to our department almost monthly, so perhaps a friendly call to your rep would be in order? Good luck, Amber From: Jay Lundgren [mailto:jaylundg...@gmail.com] Sent: Thursday, November 04, 2010 12:27 PM To: Weems, Joyce Cc: Fimbres, Amber; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Qualifications to run a Thin Prep 2000? Amber, I'm sorry if I offended anyone, I was merely referring to this instrument's wonderful ease of operation. Would you happen to know the answer to my question, as I see you are a cytotech? Jay A. Lundgren M.S., HTL (ASCP) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Qualifications to run a Thin Prep 2000?
I have sent an email to cytyc, and am awaiting a response, but I was hoping someone on Histonet knew the answer to my query. Thanks, Jay -- This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Qualifications to run a Thin Prep 2000?
I also wanted to add Jay that our lab assistants are not certified as either HT or HTL. The entry level education requirement of a lab assistant is a high school diploma (or equivalent). Of course what you want in a lab assistant is totally different (such as previous experience, knowledge of medical terminology, etc.). Thanks, Amber From: Jay Lundgren [mailto:jaylundg...@gmail.com] Sent: Thursday, November 04, 2010 1:33 PM To: Fimbres, Amber Cc: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Qualifications to run a Thin Prep 2000? I have sent an email to cytyc, and am awaiting a response, but I was hoping someone on Histonet knew the answer to my query. Thanks, Jay This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslipping Hood
Does anyone know of a coverslipping hood that has a downdraft exhaust? Any recommendations on coverslipping hoods??? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: alcoholic formalin
If they are insistent on using the alcohol try shortening or deleting some of the alcohol times on the processor. Weems, Joyce jwe...@sjha.org Sent by: histonet-boun...@lists.utsouthwestern.edu 11/04/2010 01:08 PM To Laurie Elmgren lelmg...@sunriselab.com, Histonet histonet@lists.utsouthwestern.edu cc Subject [Histonet] RE: alcoholic formalin Can you get the client to use 10% NBF? That would be the first thing I'd do. If not, I'd soak blocks. One thing you can do is throw it in your water bath for a few seconds - then put on ice. The warm water seems to work really well especially on bloody tissue. j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Elmgren Sent: Thursday, November 04, 2010 15:24 To: Histonet Subject: [Histonet] alcoholic formalin I have a client that uses alcoholic formalin as a primary fixative for small endometrial and cervical biopsies. What can I do to keep the cells from looking like raisins? We presently run these biopsies on a conventional processor with a short biopsy schedule. Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Automatic special stainer for Histology
To the Histology community; Need information on automatic special stainer that are being used in Histology community laboratory. Price per slide and reliability of the instrument as well as the stains. Thank you. Ermestine Middleton, Manager Montefiore Medical Center Histology Laboratory; Dept. Pathology Bronx, New York 718-920-4157 emidd...@montefiore.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin liver sections
Thanks to all the prompt responses. He fixed the entire liver in excess formalin (I think around 15 mL) with rocking for 3 days. I'm not sure about the processing schedule; I'll have to contact the histology core to ask. I forwarded all of the advice along, and he tried soaking the blocks in ice water for several minutes. It fixed most of the problem, at least for now. You people are far too helpful. Thanks, Adam On Thu, Nov 4, 2010 at 2:53 PM, Jay Lundgren jaylundg...@gmail.com wrote: Sounds to me like they are overprocessed (dried out). Try soaking them on ice for 30 minutes or so before cutting. Some people use a drop of fabric softener on their ice tray to soften the tissue, or there are commercial products like soft block from Polyscience. Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
