RE: [Histonet] Re: Histology Consultant
Thanks Nathan!! I just don't want to be cheated on what I will be getting paid. My old job is wanting me to come in and do the small amount of histology work they have. This would all be on evenings and weekends. It is not PRN, more like call me once a month and I will come do the work. I'm getting anywhere from $17/hr (that's insane!! How do you even sleep at night paying a registered, college educated person that? Working at Walmart would pay you more!!!) to $50/hr (which was more what I was thinking). Just trying to get a good feel, and not cheat myself or the other company. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Thursday, November 25, 2010 6:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histology Consultant If you don't know what the going rate is, that is exactly why you ask. That is what this forum is for. A little professionalism is appreciated. Nathan Jentsch BS HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD20 for IHC on mouse FFPE tissue
Thanks Guys, I plan to order one of the Rb monoclonals for CD20. Best, Brett -Original Message- From: Amos Brooks [mailto:amosbro...@gmail.com] Sent: Friday, November 26, 2010 4:58 PM To: koelli...@comcast.net Cc: Connolly, Brett M; histonet@lists.utsouthwestern.edu; peter.t...@abdserotec.com Subject: Re: [Histonet] CD20 for IHC on mouse FFPE tissue Thanks Brett, Looks like I got a couple lines crossed there. I had been told that there wasn't any CD20 in mice. I have been using B220 for BCell detection. After you pointed out the error I looked it up at Biocompare and clearly there are some CD20 markers for mice. http://tinyurl.com/38wyh42 I was also apparently wrong about BD being a Thermo company. Thanks Peter. They do distribute it, but don't own it. My mistake ... again :-) With those goofs I'll shut up now and blame the turkey Enjoy your weekend guys, Amos On Friday 26 November 2010 04:08:06 pm koelli...@comcast.net wrote: Hello, Maybe I'm remembering wrong since I deleted the original message of Bretts looking for murine CD20 on mouse FFPE (hopefully rabbit origin?). If so anti-murine CD20 that can be made to work on mouse FFPE tissues certainly exist. Have used them from BD (flow reagent), Santa Cruz and others. In Biocompare website, can see them plus new rabbit monoclonal anti-murine CD20 for paraffin, reactive to murine tissues. There are a few CD20+ murine B-cells that have no B220, and vice-versa so I would'nt look for CD45R if I wanted to find CD20+ cells. Would look for CD20+ cells. Maybe I'm remembering original post incorrectly. Ray Ray Koelling PhenoPath Labs Seattle, WA - Original Message - From: Amos Brooks amosbro...@gmail.com To: brett connolly brett_conno...@merck.com, histonet@lists.utsouthwestern.edu Sent: Thursday, November 25, 2010 3:31:27 AM Subject: [Histonet] CD20 for IHC on mouse FFPE tissue Hi Brett, CD20 is not going to work for mouse tissue. You need CD45R (clone B220). This will selectively label B cells just like CD20 in humans. I get this from BD Biosciences (Now Thermo ... again) Catalog # 347460. It is raised in Rats and works great as long as you have a good rat secondary. The one from Jackson is good. Happy Thanksgiving, Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Histology Consultant
Your situation sounds more like contract work instead of consultation work. Your have a right to expect and I suggest you must demand a fair hourly rate to compensate for your education, experience, time and effort. You must take into consideration the market hourly rate for the area your work and the degree of difficulty in labs acquiring quality technicians/technologists. In my experience, I believe that $30.00 - $33.00/hr base rate for routine Histology work is not unusual for a contract hire without benefits. I would also suggest that you set a minimum hourly guarantee of 4 hours pay each time you come in to work and negotiate for a shift differential of 10%-15% for working nights. Remember, you are producing work that the lab or pathologist will be able to charge a minimum of $100.00/case, depending on the CPT code level and not counting the number of special stains or IHC attached to each case. You should have no issue in producing no less than 40 cases in a 4 hour period (embedding, cutting and staining). You must ask for fair compensation and always provide superior quality. Your contribution to the process is essential. Additionally, if the lab was to hire a contract person from one of the placement services, they would be charged $60.00-$65.00/hr and have to make a set time commitment (typically 1-3 months). You are a known performer to the lab and they have examples of your quality, they do not have that level of knowledge or comfort if they hire an unknown contractor. You and all of us in our Histotechnology profession, as registered, trained, competent and educated technologists should expect and demand to be fairly compensated as an essential and professional member of the Anatomic Pathology lab process. William DeSalvo, B.S., HTL(ASCP) Date: Mon, 29 Nov 2010 08:31:11 -0600 From: sgoe...@mirnarx.com To: njblademas...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histology Consultant CC: Thanks Nathan!! I just don't want to be cheated on what I will be getting paid. My old job is wanting me to come in and do the small amount of histology work they have. This would all be on evenings and weekends. It is not PRN, more like call me once a month and I will come do the work. I'm getting anywhere from $17/hr (that's insane!! How do you even sleep at night paying a registered, college educated person that? Working at Walmart would pay you more!!!) to $50/hr (which was more what I was thinking). Just trying to get a good feel, and not cheat myself or the other company. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Thursday, November 25, 2010 6:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histology Consultant If you don't know what the going rate is, that is exactly why you ask. That is what this forum is for. A little professionalism is appreciated. Nathan Jentsch BS HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat Question Please
We have this model as well and like it. We got the vacuum attachment. It's not perfect, but does facilitate cleaning up OCT and tissue scraps. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 24, 2010 4:14 PM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cryostat Question Please Very consistant, easy to use. 2 drawbacks 1. We got the decontamination add-on trying to automate the CAP requirement, but now it seems Thermo says it is not an adequate process. I believe they are doing more intense studies, but for the time being, I have to decontaminate it by hand. 2. OCT and tissue scraps build up under collection device and cause the mechanism to clunk unless buildup is removed. These two things add a good chunk of time to my day. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, November 24, 2010 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Question Please Hello all Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat? If so, what is your opinion/pros and cons? Thanks so much in advance, Paula Lab Manager BP Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotechs and cytotechs wanted
Looking for experienced histology technicians and cytotechnologists in Columbus, Ohio for new GU pathology laboratory. Excellent compensation with great benefits. Experience with IHC, FISH, and GU pathology a plus. Send resume or CV to care...@aksm.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processor change
How many blocks do you guys normally process before you change the solutions in the processor? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processor change
I change solutions after 500 blocks; paraffin after 1500 blocks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processor change
Change or rotate? ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, November 29, 2010 4:18 PM To: sgoe...@mirnarx.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processor change I change solutions after 500 blocks; paraffin after 1500 blocks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1170 / Virus Database: 426/3286 - Release Date: 11/28/10 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processor change
When the processor capacity is reached, I start to rotate the reagents, discarding the first of each group, moving forward the remaining, and replacing with fresh reagents the last. René J. --- On Mon, 11/29/10, sgoe...@mirnarx.com sgoe...@mirnarx.com wrote: From: sgoe...@mirnarx.com sgoe...@mirnarx.com Subject: [Histonet] Processor change To: histonet@lists.utsouthwestern.edu Date: Monday, November 29, 2010, 4:04 PM How many blocks do you guys normally process before you change the solutions in the processor? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processor change
Nancy Klemme, can you comment on this specifically for the Tissue Tek VIP's? Does Sakura have guidelines for rotating the solutions? Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Monday, November 29, 2010 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor change How many blocks do you guys normally process before you change the solutions in the processor? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Processor change
We change based on runs, not blocks - two times a week.. Change each single solution and first of each series and rotate. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Monday, November 29, 2010 16:05 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor change How many blocks do you guys normally process before you change the solutions in the processor? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for info on SF Bay area jobs
Hello all fellow histonet users, I am thinking of relocating to the San Francisco Bay area and was wondering if any users live and work in the area? I am curious about job opportunities and pay scale and that sort of thing. Any information or advice is greatly appreciated! Thank you, allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Robert Lott
Robert - Please email me at work ( pw...@beaumont.edu ), how/where I can contact you. (Thank you to Histonet for allowing me to use this great resource to contact another histotech.) Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Nails softened up during grossing process or at the microtome bench?
I work at a derm lab, and we see 3-5 nail specimens a week on average. We use a 10% potassium hydroxide and let them soak for 1-3 hours depending on the size of the specimen. Then at the microtome after we face in, we soak the blocks in 10% ammonium hydroxide for 5-10 minutes, which softens them up nicely for cutting. We also use the ammonium hydroxide to soak specimens with a high keratin content that makes them extremely crispy at the microtome. Nathan Jentsch BS HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Procedure for Xylene and Formalin Monitoring
Hello to all in Histoland. I hope that eveyone had a good Thaksgiving. Does any one have a procedure for when you have your lab gets monitored for xylene and formalin levels. Any help would be appreciated. I am asking for another supervisor. On the anatomic checklist youhave to have evidence of monitoring. On the micro checklist you have to have a written procedure. Thanks in advance. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
