RE: [Histonet] HE Stain
Our tap water consistently reads 6.0, and has for years. We did try turning off the tap when this first began, and manually rinsing with distilled water, but saw no difference. I will try adding the HCl today with a few test slides. Will let you know how this works out. Thanks for the suggestions. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of WILLIAM DESALVO Sent: Thursday, January 20, 2011 12:26 AM To: jkier...@uwo.ca; allison_sc...@hchd.tmc.edu Cc: histonet Subject: RE: [Histonet] HE Stain I agree that the pH might be high, but I also suggest you check your water rinse on the stainer. If you are using tap water, there can be a significant fluctuation in the quality of the water and the amount of additives and impurities present at any one time can also contribute to the mucin not being rinsed away and staining. If you are using tap water, changing to distilled or dionized water might help to improve the consistency of stain results. Good luck William DeSalvo, B.S., HTL(ASCP) From: jkier...@uwo.ca To: allison_sc...@hchd.tmc.edu Date: Wed, 19 Jan 2011 16:58:57 -0500 Subject: Re: [Histonet] HE Stain CC: histonet@lists.utsouthwestern.edu Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. John Kiernan Anatomy Cell Biology University of Western Ontario London, Canada = = = - Original Message - From: Scott, Allison D allison_sc...@hchd.tmc.edu Date: Wednesday, January 19, 2011 13:01 Subject: [Histonet] HE Stain To: histonet@lists.utsouthwestern.edu Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be
[Histonet] Re: Thanks- CLARIFICATION: CAP #ANP.21382 Reagent Expiration Date.
Thank you to all the wonderful people who responded to my inquiry, It was great to see what the various protocols are in place. I will share this information, and will implement a more formalized and concise policy. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Jan 12, 2011, at 6:21 PM, Akemi Allison wrote: Thanks Laurie, and Histoland, My client does what Laurie suggests. Perhaps, I need to clarify: Please read information below: This is regarding RAW chemicals such as: Iodine crystals, Sodium Thiosulfate, Mercuric Chloride, and Copper, etc, and dry Dye Stains, that do not have expiration dates. I don't have a written policy for evaluation of these chemicals and dyes that do not have an expiration date. How do I know they can use these chemicals and dyes to make up the final end solutions. My client currently visualy inspects these chemicals and dyes to see if they appear to be OK, they put another year to review visually. How can an untrained, non-chemist, judge by visual inspecition if the chemical is OK??? I need a written policy to adhere to CAP guidelines. Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com From: Laurie Colbert laurie.colb...@huntingtonhospital.com To: Akemi Allison akemiat3...@yahoo.com Sent: Wed, January 12, 2011 3:17:20 PM Subject: RE: [Histonet] CAP #ANP.21382 Reagent Expiration Date. Hi Akemi, Our procedure for evaluating stains/reagents is to run control tissue. If the control works, the stain/reagent is good. And that is done much more often than annually - it is done every time the stain is performed. Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 12, 2011 2:07 PM To: histonet Subject: [Histonet] CAP #ANP.21382 Reagent Expiration Date. Hi Everyone in Histoland! Happy Hump day! I have 2 questions to ask you. The CAP #ANP. 21382 Reagent Expiration Date, Evidence of compliance requires a written policy for evaluating reagents lacking manufacturer's expiration date. I worked in the Biotech arena for several years in RD and manufacturing. We had procedures which were in compliance to GLP and manufacturing standards. These policies were much more rigid than for AP departments, who adher to CAP requirements. Would any of you kind people like to share a copy of your written policy with me. I would be forever grateful! Second question: How do you address powder dyes, and chemicals in crystalin and powder form, which are extremely old and do not have an expiration date? What is your criteria for passing of failing these chemicals and dyes? Do you visually inspect these chemicals and dyes on an annual basis, and if they look fine, give it another year for a visual check with next years date, or do you send them out to a company for analysis to see if they past required specifications??? That would be pretty costly! If anyone has a written procedure for this I would love to see it too! Thank you in advance for your assistance. Akemi Akemi Allison BS, HT(ASCP)HTL E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Consult Fees for IHC
Joe: There is sort of a standard payment that almost all universities use of $3000/5 days week. This represents about $75/hour and at that rate is how most consultants work. IF they ask you for your SS number then they will issue you (at the end of the year) the correspondent IRS Form. I have never had any problems with that, you will just add that income to your 1040 Form and at the end your tax deduction will be very small. You do not have to give away your knowledge, just make sure they get their moneys worth! René J. --- On Wed, 1/19/11, Joe Nocito jnoc...@satx.rr.com wrote: From: Joe Nocito jnoc...@satx.rr.com Subject: [Histonet] Consult Fees for IHC To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, January 19, 2011, 5:58 PM Greetings histo land, Hope everyone is having a great New Year. I have been asked to consult on IHC to a local firm. They asked me what my fees are. I told them we can talk about that later. I have no idea what to charge these people. I want to be fair, but I don't want to give this knowledge away (an y'all thought that this was just a petty face). Any ideas for the Texas area. This would be telephone and on site consulting for a research firm. Thanks in advance. Joe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave oven
Dorothy: Regardless of the fact that I agree with you, never ask for the support others may provide to your statements. Give your honest opinion and do not mind what others may think about it! René J. --- On Thu, 1/20/11, tracz...@aol.com tracz...@aol.com wrote: From: tracz...@aol.com tracz...@aol.com Subject: Re: [Histonet] Microwave oven To: amosbro...@gmail.com, histonet@lists.utsouthwestern.edu Date: Thursday, January 20, 2011, 12:10 AM Amos, I think you have a few misconceptions about microwave technology. Lumping laboratory grade units in with household appliances is (in my opinion) unfair. Temperature control is essential and there are microwave processors available that do it quite well. Also, how in the world can all of the buffer solution boil out of a close container? Have you looked at the modules provided by Milestone or Hacker? They all have lids! Check out the mw credentials of a company in North Carolina called CEM. Their research and manufacturing in the field of microwave technology is some of the best in the world. There are always pros cons to be considered when making equipment purchases but I believe the large net you cast damning microwave technology is short sighted. Anyone else agree? Dorothy Dorothy Traczyk MTA Histology LLC Point Pleasant, NJ 08742 -Original Message- From: Amos Brooks amosbro...@gmail.com To: casperhempel casperhem...@gmail.com; histonet histonet@lists.utsouthwestern.edu Sent: Wed, Jan 19, 2011 6:34 pm Subject: [Histonet] Microwave oven Hi, Please don't! Microwaves are horrible for HIER. For retrieval purposes, ou need to get the buffer temperature up to a point and keep it there for a ength of time without the solution boiling over and leaving you with nhappy dry sections. Microwaves heat unevenly really fast then boil over. he solution to this is to nuke it then cool it then nuke it again repeating ntil you figure it is done. Lab grade microwaves do the same thing under a it tighter control. This results in a wave of temperature fluctuation which s anything but standardizable (if that's a word). You would be much better off getting a vegetable steamer on the cheap ide, or a standard laboratory waterbath on the expensive side. These both an allow a direct monitoring of temperature throughout the retrieval rocess. Pressure cookers are viable options as they don't allow the buffers o boil over. Biocare Medical has a decent one as well as temperature strips hat allow you to know if the temperature got to a certain point and didn't xceed another. Honestly with all CAP's nonsensical prattle about tandardisation in labs I can't understand how they allow these onstrosities in modern medical care. Message: 4 ate: Tue, 18 Jan 2011 21:59:40 +0100 rom: Casper Hempel casperhem...@gmail.com ubject: [Histonet] Microwave oven o: histonet@lists.utsouthwestern.edu essage-ID: AANLkTik3+ uZ4RF7NyjQqYKCXys60CAnEwhDrfvL=j...@mail.gmail.com ontent-Type: text/plain; charset=ISO-8859-1 Dear Histonetters e are about to purchase a new microwave oven in our lab for HIER of FFPE issue. Do you have any recommendations? I'm only aware of EMS that sells an ven with temperature control. Any suggestions are welcome heers asper __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: HE Stain
Allison Scott wrote: Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas When you say it is every few slides, is there variation within multiple slides of one case? If all the slides (levels) of a case look the same, I would suspect a problem with that particular case, not the processing and not the staining. Are these all GI biopsies? Or do you see this in other needle biopsies, cervical biopsies and the like? GI biopsies tend to show hazy nuclei in epithelial cells commonly. There are several explanations and mostly are attributed to inadequate fixation. It could be you a client who has changed how they collect and fix the specimens. Are they letting them dry on a gauze before putting them in fixative? It could be you have something out of place on the tissue processor. It could be there is water under the tissue on the slide and it is subject to high heat (microwave or oven) and is cooking the cells prior to staining. As pointed out previously, it could be a problem with your hematoxylin pH. Good luck, and please let us know what the fix for this is when you get it figured out! Best wishes, Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Animal tissue processing
Caula, I have processed my fair share of animal tissue and I used the same reagents and times that I used for human tissue. With some animal organ tissue there is the possibility of drying so you need to be careful and watch out for that. You would adjust your times accordingly. I, because of circumstances, always had the animal tissue on their own run. I do not know if there are any regulations that specifically states this. Never heard of any. I'm sure our veterinary crowd can answer that best. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Gill, Caula A. cgill@marylandge neral.org To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Animal tissue processing 01/20/2011 11:48 AM Hi All, I work in a hospital where we process human tissue. As a favor to a friend the pathologist would like us to process animal tissue. My questions are could we process the animal tissue on the same processor with the human tissue? And Are there different processing times and reagents for animal tissue? Thanks for any help you can give Caula (HT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Myocardial Biopsies
My hospital has recently started performing heart transplants and we are now receiving numerous myocardial biopsies to check for rejection. One of our forensic pathologists is an expert in cardiovascular pathology and has read many biopsies over the years for other hospitals. His protocol for cutting these biopsies is very labor intensive (10 slides with 8 serial sections on each slide, some stained with HE others kept unstained and 2 for C4d immunos). I'm curious to know what other labs are doing for these biopsies in hopes of getting this protocol changes. Thanks for your help Jeff Miller Spectrum Health Grand Rapids. MI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Myocardial Biopsies
Hi Jeff, I work at Children's Memorial in Chicago and we do these routinely. We cut 5 slides with 3 sections per slide. Slides 1,3,5 are for HE and slides 2,4 we stain with the HPS stain. We do C4d but it's not done automatically. Hope this helps, Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jeffery.mil...@spectrum-health.org Sent: Thursday, January 20, 2011 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Myocardial Biopsies My hospital has recently started performing heart transplants and we are now receiving numerous myocardial biopsies to check for rejection. One of our forensic pathologists is an expert in cardiovascular pathology and has read many biopsies over the years for other hospitals. His protocol for cutting these biopsies is very labor intensive (10 slides with 8 serial sections on each slide, some stained with HE others kept unstained and 2 for C4d immunos). I'm curious to know what other labs are doing for these biopsies in hopes of getting this protocol changes. Thanks for your help Jeff Miller Spectrum Health Grand Rapids. MI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Myocardial Biopsies
We've done them for years... 6 levels on 2 slides - L1-L3, L4-L6 1st level placed near label end of the slide No unstained. We do C4d when ordered. For cases with no previous biopsies - Pick up unstained slides at level 3 for: PAS, Masson Trichrome, Iron, Congo Red and Sulfonated Alcian Blue Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jeffery.mil...@spectrum-health.org Sent: Thursday, January 20, 2011 12:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Myocardial Biopsies My hospital has recently started performing heart transplants and we are now receiving numerous myocardial biopsies to check for rejection. One of our forensic pathologists is an expert in cardiovascular pathology and has read many biopsies over the years for other hospitals. His protocol for cutting these biopsies is very labor intensive (10 slides with 8 serial sections on each slide, some stained with HE others kept unstained and 2 for C4d immunos). I'm curious to know what other labs are doing for these biopsies in hopes of getting this protocol changes. Thanks for your help Jeff Miller Spectrum Health Grand Rapids. MI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Animal tissue processing
The NSH has an Animal Processing Manual available at their WEB site. Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gill, Caula A. Sent: Thursday, January 20, 2011 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal tissue processing Hi All, I work in a hospital where we process human tissue. As a favor to a friend the pathologist would like us to process animal tissue. My questions are could we process the animal tissue on the same processor with the human tissue? And Are there different processing times and reagents for animal tissue? Thanks for any help you can give Caula (HT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C3d
Is anyone using a Commercially-available C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] flash freezing tissue
Can someone recommend the best protocol for flash freezing tissue (skin). I am unsure if fixing tissue before freezing or fixing tissue post freezing is best. Also, these samples cannot be fixed with formalin. Thanks in advance. kris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] C3d
Richard, We have used the C3d from Cell Marque for quite some time on our Bonds with very clean crisp results on all our transplant biopsies. The jury is still out on how much more information we get in addition to C4d. Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, January 20, 2011 12:26 PM To: Histonet Subject: [Histonet] C3d Is anyone using a Commercially-available C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NEW CAP?
ANP.22760 NEW REAGENT LOT VERIFICATION New lots of antibody and detection system reagents are tested in parallel with old lots. Record of validation of new reagents/shipments I was wondering if you are using Ventana xt, How are you testing each time you use new DAB-kit ( new lot#) Thanks, Behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Invitation to connect on LinkedIn
LinkedIn richard shook requested to add you as a connection on LinkedIn: -- Jackie, I'd like to add you to my professional network on LinkedIn. - richard Accept invitation from richard shook http://www.linkedin.com/e/yvpgd1-gj5ysfcb-e/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1065146327_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPsOcPoQcjkSc359bQJclTlPinFKbPgTejsPej0Ucj8LrCBxbOYWrSlI/EML_comm_afe/ View invitation from richard shook http://www.linkedin.com/e/yvpgd1-gj5ysfcb-e/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1065146327_3/3dvdP8PdzgNdjoMckALqnpPbOYWrSlI/svi/ -- DID YOU KNOW you can use your LinkedIn profile as your website? Select a vanity URL and then promote this address on your business cards, email signatures, website, etc http://www.linkedin.com/e/yvpgd1-gj5ysfcb-e/ewp/inv-21/ -- (c) 2010, LinkedIn Corporation ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Animal tissue processing
Generally rodent tissues require shorter times for processing. See the Animal Processing Manual published by the NSH for a range of tried and tested protocols. Below I have pasted a copy of my schedule which works well for mouse tissues. As you will see I designed it in the first instance for pancreas which tends to harden on longer processes, now I use it for all my mouse tissues, with the exception of intact heads (these require much longer, i.e. 2 hours per station.) All my processes are developed using the methods in the above mentioned manual as a guide. My processor is a dunk and dip type of machine, the Leica TP1020. I hope this helps. SOP 3 PROGRAMME 4: FOR MOUSE PANCREAS COSHH CBH001 STATION REAGENT DURATION VACUUM 1 Formalin/70% ethanol 30 mins Y 2 80% Ethanol 30 mins Y 3 90% Ethanol 20 mins Y 4 Absolute ethanol/IMS 20 mins Y 5 Absolute ethanol/IMS 30 mins Y 6 Absolute ethanol/IMS 30 mins Y 7 Histoclear II 20 mins Y 8 Histoclear II 30 mins Y 9 Histoclear II 30 mins Y 10 Wax 30 mins Y 11 Wax 45 mins Y 12 Wax 45 mins Y This programme can be run during the day as long as it is started early enough; if not, a delay must be set in order that the samples are not left in hot wax for extended times - refer to instrument manual. Good luck Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gill, Caula A. Sent: 20 January 2011 16:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal tissue processing Hi All, I work in a hospital where we process human tissue. As a favor to a friend the pathologist would like us to process animal tissue. My questions are could we process the animal tissue on the same processor with the human tissue? And Are there different processing times and reagents for animal tissue? Thanks for any help you can give Caula (HT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Myocardial Biopsies
7 slides, one ribbon of 6 sections/slide: HE x4, C4d x1, Masson Trichrome x1, unstained x1 Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jeffery.mil...@spectrum-health.org Sent: Thursday, January 20, 2011 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Myocardial Biopsies My hospital has recently started performing heart transplants and we are now receiving numerous myocardial biopsies to check for rejection. One of our forensic pathologists is an expert in cardiovascular pathology and has read many biopsies over the years for other hospitals. His protocol for cutting these biopsies is very labor intensive (10 slides with 8 serial sections on each slide, some stained with HE others kept unstained and 2 for C4d immunos). I'm curious to know what other labs are doing for these biopsies in hopes of getting this protocol changes. Thanks for your help Jeff Miller Spectrum Health Grand Rapids. MI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Courier Tracking Mechanism
Am looking for a method for verification of specimens for couriers/ laboratories. Anyone use such an animal? Bill, as I recall you have in use such an animal? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Courier Tracking Mechanism
Our lab here, and previous employ in Virginia, uses Gajema Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Thursday, January 20, 2011 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Courier Tracking Mechanism Am looking for a method for verification of specimens for couriers/ laboratories. Anyone use such an animal? Bill, as I recall you have in use such an animal? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] C3d
Hi Dr Cartun, I hope anyone answering this will make it available for all of HistoNet. I would be very interested in the use of this antibody as we are already using C4d IF for kidney here. Pamela Marcum UAMS Little Rock AR - Original Message - From: Richard Cartun rcar...@harthosp.org To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a Commercially-available C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Original Message - From: Richard Cartun rcar...@harthosp.org To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a Commercially-available C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] C3d
Pam, We have been performing C4d by IHC for about 3 years now and C3d for many months on all our transplant biopsies. Obviously, being able to do this on paraffin obviates the need to have additional tissue for frozens (although I do understand the need for additional IF on kidney biopsies - but we're working on that!). Currently we get both antibodies from Cell Marque and run them on our Bonds Let me know if you need any more info. Ronnie Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, January 20, 2011 1:28 PM To: Richard Cartun Cc: Histonet Subject: Re: [Histonet] C3d Hi Dr Cartun, I hope anyone answering this will make it available for all of HistoNet. I would be very interested in the use of this antibody as we are already using C4d IF for kidney here. Pamela Marcum UAMS Little Rock AR - Original Message - From: Richard Cartun rcar...@harthosp.org To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a Commercially-available C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Original Message - From: Richard Cartun rcar...@harthosp.org To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a Commercially-available C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] C3d
Our patholgoists do not feel IHC gives them the discreet staining patterns they see with IF. They have tried IHC for C4d and did not like it. The only platform we have here is Ventana and that often slows us down on things as they may not have the antibody ready to use or a protocol for it. Pam Marcum - Original Message - From: Ronald Houston ronald.hous...@nationwidechildrens.org To: Pamela Marcum mucra...@comcast.net, Richard Cartun rcar...@harthosp.org Cc: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, January 20, 2011 12:33:52 PM Subject: RE: [Histonet] C3d Pam, We have been performing C4d by IHC for about 3 years now and C3d for many months on all our transplant biopsies. Obviously, being able to do this on paraffin obviates the need to have additional tissue for frozens (although I do understand the need for additional IF on kidney biopsies - but we're working on that!). Currently we get both antibodies from Cell Marque and run them on our Bonds Let me know if you need any more info. Ronnie Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, January 20, 2011 1:28 PM To: Richard Cartun Cc: Histonet Subject: Re: [Histonet] C3d Hi Dr Cartun, I hope anyone answering this will make it available for all of HistoNet. I would be very interested in the use of this antibody as we are already using C4d IF for kidney here. Pamela Marcum UAMS Little Rock AR - Original Message - From: Richard Cartun rcar...@harthosp.org To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a Commercially-available C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Original Message - From: Richard Cartun rcar...@harthosp.org To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, January 20, 2011 11:26:29 AM Subject: [Histonet] C3d Is anyone using a Commercially-available C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. - Original Message - From: Ronald Houston ronald.hous...@nationwidechildrens.org To: Pamela Marcum mucra...@comcast.net, Richard Cartun rcar...@harthosp.org Cc: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, January 20, 2011 12:33:52 PM Subject: RE: [Histonet] C3d Pam, We have been performing C4d by IHC for about 3 years now and C3d for many months on all our transplant biopsies. Obviously, being able to do this on paraffin obviates the need to have additional tissue for frozens (although I do understand the need for additional IF on kidney biopsies - but we're working on that!). Currently we get both antibodies from Cell Marque and run them on our Bonds Let me know if you need any more info. Ronnie
RE: [Histonet] Courier Tracking Mechanism
If you mean a tracking system, we use Ventana Vantage and love it. It is a perfect tracking system but so much more. Check it out through your Ventana rep. Jan Omaha, NE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Thursday, January 20, 2011 12:19 PM To: 'Nita Searcy'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Courier Tracking Mechanism Our lab here, and previous employ in Virginia, uses Gajema Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Thursday, January 20, 2011 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Courier Tracking Mechanism Am looking for a method for verification of specimens for couriers/ laboratories. Anyone use such an animal? Bill, as I recall you have in use such an animal? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Animal tissue processing
Thanks everyone the info really helps. At this time we are not sure of the exact type of tissue but I do know that it is for research. I'll check on the regulations if any about running them together. Thanks again, Those in the northeast happy snow -Original Message- From: bsulli...@shorememorial.org [mailto:bsulli...@shorememorial.org] Sent: Thursday, January 20, 2011 11:57 AM To: Gill, Caula A. Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Animal tissue processing Caula, I have processed my fair share of animal tissue and I used the same reagents and times that I used for human tissue. With some animal organ tissue there is the possibility of drying so you need to be careful and watch out for that. You would adjust your times accordingly. I, because of circumstances, always had the animal tissue on their own run. I do not know if there are any regulations that specifically states this. Never heard of any. I'm sure our veterinary crowd can answer that best. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Gill, Caula A. cgill@marylandge neral.org To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Animal tissue processing 01/20/2011 11:48 AM Hi All, I work in a hospital where we process human tissue. As a favor to a friend the pathologist would like us to process animal tissue. My questions are could we process the animal tissue on the same processor with the human tissue? And Are there different processing times and reagents for animal tissue? Thanks for any help you can give Caula (HT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stain
Allison/Toni, Thought I'd throw this out. Maybe nonsense. If you have such acidic tap water, could there be heavy metals lead, magnesium and others (acid tap water does that) in your tap water rinse from being leached out upstream. William DeSalvo talked about the quality of tap water fluctuating. Very true. And the metals from pipes or solder , leached into water by pH6.0, turning a normal hematoxylin into something like a Weigerts hematoxylin. A kind of post-mordanting that I think some call afterchroming. Although if you tried distilled or deionized water with same results, that data wouldn't fit with this problem. And even if it just started happening, has someone recently worked on pipes upsteam of where you are and there is (are) new metals being leached into your hematoxylin rinse? pH 6 is pretty acidic water. RayKoelling PhenoPath Labs Seattle, WA - Original Message - From: Toni Rathborne trathbo...@somerset-healthcare.com To: WILLIAM DESALVO wdesalvo@hotmail.com, jkier...@uwo.ca, allison scott allison_sc...@hchd.tmc.edu Cc: histonet histonet@lists.utsouthwestern.edu Sent: Thursday, January 20, 2011 6:17:46 AM Subject: RE: [Histonet] HE Stain Our tap water consistently reads 6.0, and has for years. We did try turning off the tap when this first began, and manually rinsing with distilled water, but saw no difference. I will try adding the HCl today with a few test slides. Will let you know how this works out. Thanks for the suggestions. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of WILLIAM DESALVO Sent: Thursday, January 20, 2011 12:26 AM To: jkier...@uwo.ca; allison_sc...@hchd.tmc.edu Cc: histonet Subject: RE: [Histonet] HE Stain I agree that the pH might be high, but I also suggest you check your water rinse on the stainer. If you are using tap water, there can be a significant fluctuation in the quality of the water and the amount of additives and impurities present at any one time can also contribute to the mucin not being rinsed away and staining. If you are using tap water, changing to distilled or dionized water might help to improve the consistency of stain results. Good luck William DeSalvo, B.S., HTL(ASCP) From: jkier...@uwo.ca To: allison_sc...@hchd.tmc.edu Date: Wed, 19 Jan 2011 16:58:57 -0500 Subject: Re: [Histonet] HE Stain CC: histonet@lists.utsouthwestern.edu Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. John Kiernan Anatomy Cell Biology University of Western Ontario London, Canada = = = - Original Message - From: Scott, Allison D allison_sc...@hchd.tmc.edu Date: Wednesday, January 19, 2011 13:01 Subject: [Histonet] HE Stain To: histonet@lists.utsouthwestern.edu Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.
Re: [Histonet] Courier Tracking Mechanism
Which is the animal, the courier or the method? René J. --- On Thu, 1/20/11, Nita Searcy nsea...@swmail.sw.org wrote: From: Nita Searcy nsea...@swmail.sw.org Subject: [Histonet] Courier Tracking Mechanism To: histonet@lists.utsouthwestern.edu Date: Thursday, January 20, 2011, 1:15 PM Am looking for a method for verification of specimens for couriers/ laboratories. Anyone use such an animal? Bill, as I recall you have in use such an animal? Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] NEW CAP?
We have selected a standard antibody panel that we run and have the pathologist review when we get a new lot in. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Thursday, January 20, 2011 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NEW CAP? ANP.22760 NEW REAGENT LOT VERIFICATION New lots of antibody and detection system reagents are tested in parallel with old lots. Record of validation of new reagents/shipments I was wondering if you are using Ventana xt, How are you testing each time you use new DAB-kit ( new lot#) Thanks, Behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Training Plan for Microtomy
I was wondering if any of you may have a microtomy training plan in your files that you would be willing to share? We are looking to expand some of the duties of some of our techs and would like to start things in motion by putting in place a good training plan prior to anyone getting near anything sharp. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Training Plan for Microtomy
Stephen There are three excellent references. They all give much more than students need but provide nice context. The Effective Use and Proper Care of the Microtome Oscar W. Richards. Published by American Optical Company. Not sure when last published but my copy is 1959. This gives the theory and the history of microtomes and microtomy. The Microtome. A Guide to Specimen Preparation and Sectioning. Walter F. Published by the Leitz Company. Section Cutting in Microscopy. Steedman H.F. 1960 Blackwell Scientific Publications, Oxford. Great description of development of embedding media . Barry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Thursday, January 20, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training Plan for Microtomy I was wondering if any of you may have a microtomy training plan in your files that you would be willing to share? We are looking to expand some of the duties of some of our techs and would like to start things in motion by putting in place a good training plan prior to anyone getting near anything sharp. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Training Plan for Microtomy
Stephen Sorry I forgot If you want a bare bones approach then Culling's book Cellular Pathology Technique has a good chapter on this. Published by Butterworths Barry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Thursday, January 20, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training Plan for Microtomy I was wondering if any of you may have a microtomy training plan in your files that you would be willing to share? We are looking to expand some of the duties of some of our techs and would like to start things in motion by putting in place a good training plan prior to anyone getting near anything sharp. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] OHIO Position
Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a full time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. To learn more about Avamar Gastroenterology please visit their website at www.avamargasto.com. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mh...@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mh...@carisls.com mailto:mh...@carisls.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] General supervisor requirement for CA and CLIA.
Hi CA Histology Manager's, What is your understanding of who can supervisor the IHC department as a general supervisor? Especially for licensed States like CA. At Cedars they had the IHC lab under the supervision of CLS tech and Managers. I believe they ran into issues due to the high complexity testing issues. My client will need to get on the same page on how they are meeting the general supervisor requirement for CA and CLIA. Thanks, Akemi Allison BS, HT(ASCP)HTL Director PhoenixLab Consulting E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Consult fees
Just wanted to thank all of you for your responses. I may be a little sentimental and biased, but this is still a great forum. You all are great. I belong to another list server (which will left unnamed) and that other place doesn't even come close to the Histonet. Crap, I feel a tear or two coming on. Now I can't see the computer screen. Got to go and thanks again Joe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Animal tissue
If you are processing mammalian tissue, it can be run with your regular human' tissues. Rodent tissues are totally different and require shorter processing times. As long as your animal tissues are grossed at the proper thickness you should have no trouble. Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] in situ hybridization
Hi all, after a decade of not doing ISH, I have been asked to initiate a project in our department. needless to say i am VERY rusty. can any one suggest: - a good on-line resource to brush up my knowledge - a company that have reference materials/handbooks etc (I seem to recall Roche did this)? - A course/workshop that i can attend specifically on embryo and bone in situ? As always i thank the forum for its help best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
