[Histonet] Mercury free Gilson's fluid
Hello all, Does anyone know about a Gilson's fluid formula which is mercury-free? I've been searching and I've found a paper on it in Histo-Logic Vol VI, Num 4, October 1976 by Carolyn A. Barsczcz who reccomends the use oz zinc chloride in substitution of mercury chloride in zenker type fixatives (Gilson's fluid seems to be of this kind), but I haven't been able to find formulas. ¿Any suggestion? Thank you very much Jorge Tornero ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: paraffin sections: white with airbubbles
Hi An, You did not mention a clearing step (xylene, or xylene substitute) between your alcohol and paraffin steps. Maybe you just forgot to write it, but if you actually missed this step, your samples would be improperly processed leading to the problems you have. Nancy Thomas Stowers Institute for Medical Research Kansas City, Mo -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of An Eerdekens Sent: Friday, January 28, 2011 4:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin sections: white with airbubbles Dear collegues, I am experiencing following problem. I have embedded hypothalamus tissue in paraffin using the following procedure: -fixation in 4% paraformaldehyde for 48 hours, fixation of the tissue in 50% alcohol, next day in 70% alcohol,next day paraffin embedding. During the paraffin embedding there was a short circuit and the machine did not work for any hours, so there was a delay in the process. Now I am making slices of 5 micrometer, using the Microm HM 360. The tissue is very white (looks like I am making much thicker sections) on the slices with airbells inside. I don't have an explanation for this and many samples are showing the same features. Does someone know what might be the reason? Thanks for the help. Regards, An Eerdekens Laboratory of Intensive Care Medicine Catholic University Leuven, Belgium 003216330518 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] OFF-TOPIC - stuff to do in New Orleans
Hi Guys, I am sure that there are New Orleans histonetters out there. I am going there in a couple of weeks for a few days with the kids (boys, age 9 and 14). I was wondering if anyone could suggest some good places to go, for both kids and grownups (we are going with other families and have built-in babysitters). Thanks a bunch, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] oct effect on hydroxyproline assays?
Do you know if people inflate with OCT and then successfully get protein out of the tissue or look at collagen via hydroxyproline assay? I know we have been able to extract mRNA but not sure if the gycols in oct would have any effect on getting protein out to look at collagen via the assay mentioned. Many thanks to the histo geniusi out there. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Invitation to connect on LinkedIn
LinkedIn James Dooley requested to add you as a connection on LinkedIn: -- Jackie, I'd like to add you to my professional network on LinkedIn. - James Accept invitation from James Dooley http://www.linkedin.com/e/yvpgd1-gjh624wd-5a/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1084711877_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPsTe34NdPgUc359bR5NrCdve6NVbP0OejsUcjAOcz8LrCBxbOYWrSlI/EML_comm_afe/ View invitation from James Dooley http://www.linkedin.com/e/yvpgd1-gjh624wd-5a/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1084711877_3/3dvdPsUcj4Td3wMckALqnpPbOYWrSlI/svi/ -- DID YOU KNOW LinkedIn can help you find the right service providers using recommendations from your trusted network? Using LinkedIn Services, you can take the risky guesswork out of selecting service providers by reading the recommendations of credible, trustworthy members of your network. http://www.linkedin.com/e/yvpgd1-gjh624wd-5a/svp/inv-25/ -- (c) 2011, LinkedIn Corporation ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LEAN Processes/ new lab
Hi Histonetters- I am looking for input, pros and cons or anything else you can offer. We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes. What would you do differently? What would you do the same? Are you in the tri-state (IA, WI, MN) area and open to visitors? Thank you in advance for your thoughts- Nancy Schmitt MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] LEAN Processes/ new lab
Hi Nancy, We built a brand new pathology lab from the bottom up last year. Drop me an email off line or give me a call and we can discuss our lessons learned etc. I worked with a LEAN workflow specialist on the design and equipment placement prior to the plans being approved. We included the architect in all of out discussions about this. You can always come to snowy New Hampshire and check it out yourself? Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, January 28, 2011 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LEAN Processes/ new lab Hi Histonetters- I am looking for input, pros and cons or anything else you can offer. We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes. What would you do differently? What would you do the same? Are you in the tri-state (IA, WI, MN) area and open to visitors? Thank you in advance for your thoughts- Nancy Schmitt MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Any problems with Bond Max Heater Assembly (SSA)
Has anyone had any problems with the Leica Bond Max's Heater assembly. We have 2 Bonds that are less than 2 years old and one of the heater assembles went. That is 10 less slides that we have to work with. Thank you, Dana Settembre University Hospital - UMDNJ Newark, NJ 973-972-5232 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Friday, January 28, 2011 10:06 AM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] LEAN Processes/ new lab Hi Nancy, We built a brand new pathology lab from the bottom up last year. Drop me an email off line or give me a call and we can discuss our lessons learned etc. I worked with a LEAN workflow specialist on the design and equipment placement prior to the plans being approved. We included the architect in all of out discussions about this. You can always come to snowy New Hampshire and check it out yourself? Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, January 28, 2011 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LEAN Processes/ new lab Hi Histonetters- I am looking for input, pros and cons or anything else you can offer. We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes. What would you do differently? What would you do the same? Are you in the tri-state (IA, WI, MN) area and open to visitors? Thank you in advance for your thoughts- Nancy Schmitt MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Any problems with Bond Max Heater Assembly (SSA)
We have a Bond III that's about a year old. I'm also interested in the answer. What does Leica say is the normal replacement period for this item? Is it covered under your service contract? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Settembre, Dana Sent: Friday, January 28, 2011 10:13 AM To: 'Feher, Stephen'; Nancy Schmitt; histonet@lists.utsouthwestern.edu Cc: Delia, Catherine Subject: [Histonet] Any problems with Bond Max Heater Assembly (SSA) Importance: High Has anyone had any problems with the Leica Bond Max's Heater assembly. We have 2 Bonds that are less than 2 years old and one of the heater assembles went. That is 10 less slides that we have to work with. Thank you, Dana Settembre University Hospital - UMDNJ Newark, NJ 973-972-5232 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Friday, January 28, 2011 10:06 AM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] LEAN Processes/ new lab Hi Nancy, We built a brand new pathology lab from the bottom up last year. Drop me an email off line or give me a call and we can discuss our lessons learned etc. I worked with a LEAN workflow specialist on the design and equipment placement prior to the plans being approved. We included the architect in all of out discussions about this. You can always come to snowy New Hampshire and check it out yourself? Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, January 28, 2011 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LEAN Processes/ new lab Hi Histonetters- I am looking for input, pros and cons or anything else you can offer. We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes. What would you do differently? What would you do the same? Are you in the tri-state (IA, WI, MN) area and open to visitors? Thank you in advance for your thoughts- Nancy Schmitt MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Any problems with Bond Max Heater Assembly (SSA)
Hi Folks, Someone from Leica should answer this question for you (Sara, are you listening in?), but I have a Bondmax and from time-to-time one of the heating pads will burnout. The other 9 positions continue to work. It used to be that the whole assembly was automatically replaced but now they manufacture them in such a way that an individual heater pad can be replaced. If you have a service contract this work will be covered. I think one or two heating pad errors per year could be expected - at least that has been my experience, but as I said, it doesn't slow us down much because the other 9 positions remain functional. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Rathborne, Toni trathbo...@somerset-healthcare.com 1/28/2011 11:17 AM We have a Bond III that's about a year old. I'm also interested in the answer. What does Leica say is the normal replacement period for this item? Is it covered under your service contract? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Settembre, Dana Sent: Friday, January 28, 2011 10:13 AM To: 'Feher, Stephen'; Nancy Schmitt; histonet@lists.utsouthwestern.edu Cc: Delia, Catherine Subject: [Histonet] Any problems with Bond Max Heater Assembly (SSA) Importance: High Has anyone had any problems with the Leica Bond Max's Heater assembly. We have 2 Bonds that are less than 2 years old and one of the heater assembles went. That is 10 less slides that we have to work with. Thank you, Dana Settembre University Hospital - UMDNJ Newark, NJ 973-972-5232 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Friday, January 28, 2011 10:06 AM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] LEAN Processes/ new lab Hi Nancy, We built a brand new pathology lab from the bottom up last year. Drop me an email off line or give me a call and we can discuss our lessons learned etc. I worked with a LEAN workflow specialist on the design and equipment placement prior to the plans being approved. We included the architect in all of out discussions about this. You can always come to snowy New Hampshire and check it out yourself? Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, January 28, 2011 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LEAN Processes/ new lab Hi Histonetters- I am looking for input, pros and cons or anything else you can offer. We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes. What would you do differently? What would you do the same? Are you in the tri-state (IA, WI, MN) area and open to visitors? Thank you in advance for your thoughts- Nancy Schmitt MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext.
[Histonet] Histotech with Hospital Experience
Still have an immediate need in the East Bay of San Francisco for a histotech. Must have hospital experience. Hours can be flexible. Ongoing contract position. Great opportunity! Contact me today for more details! Heidi Hawthorne Sr. Account Executive On Assignment, Inc. t: (510) 663-8622 c: (510) 435-7326 f: (866) 741-0805 heidi.hawtho...@onassignment.commailto:heidi.hawtho...@onassignment.com www.onassignment.comhttp://www.onassignment.com/ NASDAQ: ASGN People First. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] LEAN Processes/ new lab
1-Make sure that your instruments are located in the same sequence as your procedure allowing the readily transfer of specimens between them. 2-Get a tissue processor and processing protocol that will allow you to spend the same time preparing and processing the specimens, as the time it takes you to finish the slides. 3-Write cassettes and slides automatic, and reader for both in each cutting station. 4-Automate staining (all types) and coverslipping, and 5-Delegate all non technical tasks to assistants René J. --- On Fri, 1/28/11, Nancy Schmitt nancy_schm...@pa-ucl.com wrote: From: Nancy Schmitt nancy_schm...@pa-ucl.com Subject: [Histonet] LEAN Processes/ new lab To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Friday, January 28, 2011, 9:47 AM Hi Histonetters- I am looking for input, pros and cons or anything else you can offer. We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes. What would you do differently? What would you do the same? Are you in the tri-state (IA, WI, MN) area and open to visitors? Thank you in advance for your thoughts- Nancy Schmitt MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Careers Bulletin 01/28/2011
Hi Histonetters! Did you make a New Years Resolution? How is it going so far? Of course I resolved that I will try to eat right and exercise and I am trying. But my most important resolution is to help even more histo techs find the right opportunity in the right place at the right time. In 2010 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get better salaries, shifts and benefits. In 2011 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately their quality of life. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. Here is a list of my current openings: HISTOLOGY/MANAGEMENT NC Histology Lab Manager Western NC NH- Histology Supervisor - Manchester, NH CA - Histology Lab Manager Central Valley of CA LA - Histology Supervisor Baton Rouge, LA HISTOTECHNICIANS/HISTOTECHNOLOGISTS NC Western NC TX East Texas will consider new grads TX Austin Histotechnician/Histotechnologist TN Nashville Histotechnician. NY Long Island Histotechs NYS license req. NY Long Island Cyto prep techs NYS license req. NY Long Island Electron Microscopy Specialist NYS license req. NY-Orange/Rockland County NYS license req. NY Rochester Dermpath Histotech NYS license required WI Lead Histotech/Immunohistochemistry Specialist Of course I cant put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. Also remember I have new positions coming in on an almost daily basis so if the area you desire is not listed dont worry we can launch a specific search for you for your preferred location remember it is free of charge and it never hurts to look. So if you or anyone you know has resolved to make a job change this year please let me know. We can get started right away or we can map out a strategy for when the timing is right. Shoot me an e-mail at rel...@earthlink.net or call me toll free at 866-607-3542. There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 9 years I have dedicated my practice solely to placing histology professionals like you. Thanks again and I hope to hear from you soon, Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] KC Dermpath job
I remember a post about a dermpath job opening. Is this still available? Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ventilation and exposure
Yesterday I asked for some direction on ventilation firms to evaluate my lab and the fumes. I think I've got some decent leads but I have another quick question, does any change the processor bottles, formalin, alcohols, xylem/subs, inside a ventilated hood? Thank for your input, Curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ventilation and exposure
Curt We fill our bottles in the hood. They are emptied into a vented flammable cabinet that contains a 55 gallon drum. You know you can do your own chemical badges for both xylene and formalin. I like to run a couple TWA (8 hour) exposures and then I badge a lot of STEL (15 minutes) exposures on what I think are worse case scenarios or where I think individuals have exposure for a short period of time, like changing the tissue processor, or loading the tissue processor, changing the stainer, manually coverslipping, grossing in tissues, etc. If you are a small business OSHA has support services. They will come to your lab and perform an audit and review your safety practices (non punitive) and give you a report where they think you can improve. It was helpful to us. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Friday, January 28, 2011 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ventilation and exposure Yesterday I asked for some direction on ventilation firms to evaluate my lab and the fumes. I think I've got some decent leads but I have another quick question, does any change the processor bottles, formalin, alcohols, xylem/subs, inside a ventilated hood? Thank for your input, Curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ventilation and exposure
Our 2 closed system processors are located under a hood. We do a remote drain and fill. This is all completed under the hood. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Curt Tague c.tague@patholog yarts.com To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] ventilation and exposure 01/28/2011 12:23 PM Yesterday I asked for some direction on ventilation firms to evaluate my lab and the fumes. I think I've got some decent leads but I have another quick question, does any change the processor bottles, formalin, alcohols, xylem/subs, inside a ventilated hood? Thank for your input, Curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] UAMS Jobs for Histology
Sorry it would not go through for me on a regular post. Pam Good Morning, We have two positions open in Little Rock, Arkansas at UAMS. I will preface this by saying we are not able to pay relocation or other bonuses so please be aware of this before answering. I will also state we are not allowed to use the services of out side placement services so please do not reply as I cannot (even if I want to) use your help. If you are interested please send me a resume or CV and I will call you. 1. We need a Histology Supervisor with a minimum of 5 years supervisory experience and must Ht or HTL certified. This is a position that will require 60% bench work in the laboratory. The salary levels are set by the state of Arkansas so the negotiating is limited. I want to be completely honest in what we can do and mislead anyone. 2. We need a histologist with IHC experience in our IHC department. The person must be an HT, perferably with experience in this area. Some rotation with Histology is possible. We currently have Ventana instruments so a familiarity with the equipment would be helpful. General Histology experience preferred in the clinical area. Pamela Marcum UAMS Anatomic Pathology Manager - Original Message - From: Pam Barker rel...@earthlink.net To: Histonet histonet@lists.utsouthwestern.edu Sent: Friday, January 28, 2011 10:51:21 AM Subject: [Histonet] RELIA Histology Careers Bulletin 01/28/2011 Hi Histonetters! Did you make a New Year’s Resolution? How is it going so far? Of course I resolved that I will try to eat right and exercise and I am trying. But…my most important resolution is to help even more histo techs find the right opportunity in the right place at the right time. In 2010 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get better salaries, shifts and benefits. In 2011 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately their quality of life. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. Here is a list of my current openings: HISTOLOGY/MANAGEMENT NC – Histology Lab Manager – Western NC NH- Histology Supervisor - Manchester, NH CA - Histology Lab Manager – Central Valley of CA LA - Histology Supervisor – Baton Rouge, LA HISTOTECHNICIANS/HISTOTECHNOLOGISTS NC – Western NC TX – East Texas will consider new grads TX – Austin – Histotechnician/Histotechnologist TN – Nashville Histotechnician. NY – Long Island Histotechs NYS license req. NY – Long Island Cyto prep techs NYS license req. NY – Long Island Electron Microscopy Specialist NYS license req. NY-Orange/Rockland County NYS license req. NY – Rochester Dermpath Histotech NYS license required WI – Lead Histotech/Immunohistochemistry Specialist Of course I can’t put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. Also remember I have new positions coming in on an almost daily basis so if the area you desire is not listed don’t worry we can launch a specific search for you for your preferred location remember it is free of charge and it never hurts to look. So if you or anyone you know has resolved to make a job change this year please let me know. We can get started right away or we can map out a strategy for when the timing is right. Shoot me an e-mail at rel...@earthlink.net or call me toll free at 866-607-3542. There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 9 years I have dedicated my practice solely to placing histology professionals like you. Thanks again and I hope to hear from you soon, Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
[Histonet] Gilson's fluid
Jorge Tornero asks about Gilson's fluid. The original Gilson's fluid was made up of Mercuric chloride 2 grams, glacial acetic acid 0.4ml, nitric acid 1.8 ml, 95% ethyl alcohol 10 ml, and distilled water 88ml. I don't know about usining zinc chloride in the formula Michael Titford USA Pathology Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] LEAN Processes/ new lab
Hi Nancy, For labeling and identification our company provides a LEAN solution that allows you to print-on-demand using barcodes from cassettes, specimen containers, or requisition forms. This can eliminate batch processing, keyboard entry, handwriting, and adhesive labels from your workflow. Patient information is drawn from your LIS so there's always a single data source for everything that gets printed. The solution works for histology and cytology (including Hologic ThinPrep and BD Focal Point processors). The solution, overall, provides a LEAN, Zero Inventory, workflow with On-Demand printing at the microtome or cytology processor. I'd be happy to answer any questions or go into greater detail about how a process like this can work for you. Feel free to contact me if you would like to know more. Best Regards, Brian Brian Wood President Detangle IT Tel 516-594-9344 br...@detangleit.commailto:br...@detangleit.com www.detangleIT.comhttp://www.detangleIT.com From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Friday, January 28, 2011 10:06 AM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] LEAN Processes/ new lab Hi Nancy, We built a brand new pathology lab from the bottom up last year. Drop me an email off line or give me a call and we can discuss our lessons learned etc. I worked with a LEAN workflow specialist on the design and equipment placement prior to the plans being approved. We included the architect in all of out discussions about this. You can always come to snowy New Hampshire and check it out yourself? Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.orgmailto:sfe...@cmc-nh.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, January 28, 2011 9:47 AM To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] LEAN Processes/ new lab Hi Histonetters- I am looking for input, pros and cons or anything else you can offer. We are designing a new histology lab to move to and I am interested in how others have handled this along with using LEAN processes. What would you do differently? What would you do the same? Are you in the tri-state (IA, WI, MN) area and open to visitors? Thank you in advance for your thoughts- Nancy Schmitt MLT, HT (ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message scanned by Reflexion Total Control___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] fixation question for IHC
Jan, Depends on your material, further processing and antigens. Surprisingly, some antigens are better preserved after formaldehyde fixation than after buffered formalin (probably, due to high sensitivity of particular epitope to alcohol traces from standard formalin solution). I know at least one - mouse EpCAM epithelial marker almost negative after formalin and quite well stained after formaldehyde (rat monoclonal anti EpCAM antibody G8.8 from Developmental Studies Hybridoma Bank). Morphology and antigen staining on cryo-sections from formaldehyde-fixed mouse embryos (e11-e16) are slightly better than after formalin fixation (used the same 4-6h fixation time either with buffered formalin or with 4% formaldehyde on PBS prepared from 20% formaldehyde water solution from Electron Microscopy Sciences, following PBS wash and storage in PBS at +4 C). It seems, embryonic tissue is quite sensitive to coagulation induced by the presence of small amount of alcohol. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Berry Sent: Friday, January 28, 2011 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fixation question for IHC Is there a difference between using paraformaldehyde and neutral buffered formalin when choosing a fixative for IHC? I would prefer to use formalin because of easier preparation, but am willing to put in the extra time to make fresh paraformaldehyde solution if there is a compelling reason. Jan Berry University of Michigan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Any problems with Bond Max Heater Assembly (SSA)
Hi, I see this issue from time to time. One thing to note, does the same heater stay out or do they jump around? If they jump around, this is probably due to an issue with the power for the instrument. Make sure all your line conditioners and battery backups are performing properly and this will fix it. If the same heater is going out, the heater is definitely bad and should be replaced. We have had a couple go out and it is a relatively easy fix, even though it can be a bit of a bother until they get replaced. Good luck! Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] OHIO
Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a full time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. To learn more about Avamar Gastroenterology please visit their website at www.avamar.com Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mh...@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mh...@carisls.com mailto:mh...@carisls.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Mercury free Gilson's fluid
Gilson - whoever he may have been - the name is probably French, pronounced zheelSAW - apparently published both mercury and zinc forms of the fixative, more than a century ago. My source is the venerable Microtomist's Vade-Mecum, 11th ed. 1950, pages 40 and 43. The mercury formula can be conveniently accessed at http://stainsfile.info/StainsFile/prepare/fix/fixatives/gilson.htm Is there enough nitric acid in the brew to interfere with IHC? The zinc formula uses glacial acetic acid 5 mL 80% nitric acid 5 mL 80% alcohol 100 mL distilled water 300 mL dry zinc chloride 20 grams Published by Gilson in La Cellule, vi, 1890, page 122. Petrunkevitch's fixative is closely related, both historically and in composition. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: fixation question for IHC (Jan Berry)
I think it is a very small minority of markers that would be sensitive to the methanol residues present in formalin. So it depends on what you plan on staining. For general purposes, I would always go with formalin, unless there is a proven need for formaldehyde. __ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Québec, Canada J7H 1N8 tel: 450-435-9482 ext.247 fax: 450-435-4795 jm.lapoi...@accellab.com -- Message: 3 Date: Fri, 28 Jan 2011 13:15:19 -0500 From: Jan Berry jebe...@umich.edu Subject: [Histonet] fixation question for IHC To: histonet@lists.utsouthwestern.edu Message-ID: 1ba205bb-22b9-4167-a36d-01b7f3f04...@umich.edu Content-Type: text/plain; charset=us-ascii Is there a difference between using paraformaldehyde and neutral buffered formalin when choosing a fixative for IHC? I would prefer to use formalin because of easier preparation, but am willing to put in the extra time to make fresh paraformaldehyde solution if there is a compelling reason. Jan Berry University of Michigan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Best Ever Oil Red O Protocol
Ada Feldman sent me an article on Oil Red O staining and I wrote the following protocol for my lab based on the article. It works exceptionally well, frozen adrenal controls have brightly stained positive cortex and clear negative medulla. Best of all it has no precipitate like all the other methods. It also birefringent under polarizing filters. This is the best Oil Red O protocol anywhere! Our lab has tried several water based mounting mediums and the warm glycerin jelly works best. With glycerin jelly the stain and hematoxylin fades very little, even after a week. Also the cover glass doesnt slide around because the jelly firms up when it cools. OIL RED O IN TRIETHYL PHOSPHATE From: Ada T. Feldman and Richard W. Dapson (1974) Medical Laboratory Technology 31, 335-341, RELATIVE EFFECTIVENESS OF VARIOUS SOLVENTS FOR OIL RED O, Department of Biology, University of Michigan-Flint, Flint, Michigan 48503, U.S.A. Triethyl Phosphate (Spectrum T2256, 500ML, CAS 78-40-0) VWR cat. # 76-688 Oil Red O (Alfa Aesar CAS# 1320-06-5, 25G, C.I. 26125) VWR cat # AAA12989-14 Glycerin Jelly Mounting Medium (Electron Microscopy Sciences, Cat. # 17998-10, 100 ml) VWR cat# 17998-10 VWR Harris Hematoxylin (VWR Premium Stains, 95057-858) VWR cat # 95057-858 Lithium Carbonate (TCI America, 500 G) VWR cat TCL0224-500G Disposable Transfer pipettes VWR cat# 16001-180 VWR Superfrost Plus Microscope slides (VWR 25X75MM PK72) VWR cat# 48311-703 VWR® Micro Cover Glasses, Rectangular, No. 11/2 VWR cat# 48393-194 Solutions: 60% Triethyl Phosphate 600 ml Trithyl Phosphate 400 ml Distilled Water 0.5% Oil Red O in 60% Triethyl Phosphate 300 ml 60% Triethyl phosphate 1.5 gm Oil Red O Harris Hematoxylin Saturated Lithium Carbonate 18 gm lithium carbonate 1500 ml Distilled Water Technic: 1. Pre-warm Glycerin Jelly in 60°C waterbath 30mins prior to staining. 2. Bring frozen sections to room temperature. 3. 60% Triethyl Phosphate a few dips. 4. Stain sections in 0.5% Oil Red O, 15 to 20 minutes. 5. Rinse in water 2 minutes. 6. Counterstain in filtered Harris Hematoxylin, 2 minutes. 7. Blue in saturated Lithium Carbonate solution, 10 seconds 8. Rinse in water 5 minutes and hold in water. 9. Mount with warm Glycerin Jelly using a clean transfer pipette. Take care to avoid contaminating the pipette and Glycerin Jelly. Results: Fat Pink, Red, Bright Orange Nuclei - Blue Donna J. Emge, ASCP-HT Mouse Histology and Phenotyping Laboratory Manager Northwestern University Olson Pavilion 8-333 710 North Fairbanks Court Chicago, IL 60611 d-e...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Mercury free Gilson's fluid
Hello, Robert, Michael and all those keeping an eye on this list, Thank you very much for your fast (instant, I'll say, taking into account our respective timezones) answer to my question. Best regards, Jorge Tornero Instituto Español de Oceanografía-Cádiz Spain www.ieo.es 2011/1/28 Robert Richmond rsrichm...@gmail.com Gilson - whoever he may have been - the name is probably French, pronounced zheelSAW - apparently published both mercury and zinc forms of the fixative, more than a century ago. My source is the venerable Microtomist's Vade-Mecum, 11th ed. 1950, pages 40 and 43. The mercury formula can be conveniently accessed at http://stainsfile.info/StainsFile/prepare/fix/fixatives/gilson.htm Is there enough nitric acid in the brew to interfere with IHC? The zinc formula uses glacial acetic acid 5 mL 80% nitric acid 5 mL 80% alcohol 100 mL distilled water 300 mL dry zinc chloride 20 grams Published by Gilson in La Cellule, vi, 1890, page 122. Petrunkevitch's fixative is closely related, both historically and in composition. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
