RE: [Histonet] procedural safeguards when accessioning identical sources
We use different colored cassettes. We have 6 different colors and rotate them with the cases. The color of the cassette is also dictated as part of the gross description. We use the same color of slides as the cassettes to carry this one step further. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, February 24, 2011 2:48 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] procedural safeguards when accessioning identical sources Our lab has the policy of not accessioning back to back specimens of the same source. This helps us to ensure if there is a mix up the pathologist can tell when reading the case. For example if the specimen source is a skin and they have an endocervical they would know. We are soon to be getting a higher volume of prostates and will have to accession them together. What kind of procedural safeguards do you have in place when working with a high volume of identical sources? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] leaking specimens
Happy Friday Histonet! I'm wondering how folks out there deal with specimen containers that are leaking when received into the lab. Do you inform the physician's office, send it back, not inform anyone, etc? Thanks! Stacy McLaughlin, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Question from LIS vendor
Yes. After the final report is signed out, any changes, additions, or corrections have to be put through as an addendum. This becomes part of the patient's permanent record. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of William Shipley Sent: Thursday, February 24, 2011 11:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question from LIS vendor Hi, guys, I represent an LIS vendor in the clinical environment. We have a number of customers doing histology on our system, although it's primarily clinical in design. As we keep getting nudged in this direction, I've signed on to sort of 'soak up' the background. I've learned more about stains than I knew was possible already! One of our largest customers is in Malaysia and is doing a rather large amount of histology reports in addition to chemistry and hematology. During an inspection, an issue came up that we are trying to learn about. They took exception to the ability to alter a histology report once it had been 'finaled'. We've been told that it was unacceptable to even correct spelling errors and that any subsequent reporting had to be a separate report referring to the original. Is that practice in the US? I would have expected to have heard about it before. Can someone help me out? William Shipley Schuyler House ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] procedure guidelines
We are in the process of revamping our procedures to be in the same format throughout the entire laboratory. I have been asked to find out if there is any format guidelines particularly for histology. We are using the CLSI standards but histo and cyto are finding that we don't quite fit the mold Thank you in advance, Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC Equipment
Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC Equipment
The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Sheila Fonner sfon...@labpath.com 2/25/2011 10:20 AM Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diff quik and rapid HE billing for touch preps
Happy Friday! I am wondering if anyone can give me feedback in regards to touch prep billing when using diff quik and rapid HE staining. Our facility uses both during frozen section. I am wondering if I can bill 88333 twice per part (one for rapid HE, one for Diff quik) and then additional stains as 88334. Since they are two separate stains, even though they are touch preps, I am thinking I should be able to bill them individually as 88333 and if I do additional stains they would be billed as 88334. Any feedback is greatly appreciated. Thanks! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cdj
Now I'm curious!! Please let us know what this mysterious processing is!! Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Thu, Feb 24, 2011 at 6:46 PM, Pamela Marcum mucra...@comcast.net wrote: The CDC website can tell you exactly what to do with a CJD case and it will surprise you. Pam Marcum UAMS - Original Message - From: Kimberly K Marshall kkmarsh...@anthc.org To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 24, 2011 4:06:53 PM Subject: [Histonet] cdj Hello everyone We recently recieved a case possible positive for CJD. In researching this we have found that they now say Formalin is BAD. As long as I have been a Histo tech it seems the rules were Formalin then Formic acid. But seems there are some studies saying this is no longer enough. Is there anyone out there that has changed and if so What are you doing now??? Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Original Message - From: Kimberly K Marshall kkmarsh...@anthc.org To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 24, 2011 4:06:53 PM Subject: [Histonet] cdj Hello everyone We recently recieved a case possible positive for CJD. In researching this we have found that they now say Formalin is BAD. As long as I have been a Histo tech it seems the rules were Formalin then Formic acid. But seems there are some studies saying this is no longer enough. Is there anyone out there that has changed and if so What are you doing now??? Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cdj
The handling of CJD and other prion diseases is on the CDC website and that is the best place to understand the issues with equipment usage and contamination as well as procedures. Many people have made comments however; the true issue is not just how to handle it but what you can not use again after you have used it for a CJD case or must quarantine and not use again for routine work. Pam Marcum UAMS - Original Message - From: Emily Sours talulahg...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Friday, February 25, 2011 8:46:44 AM Subject: Re: [Histonet] cdj Now I'm curious!! Please let us know what this mysterious processing is!! Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Thu, Feb 24, 2011 at 6:46 PM, Pamela Marcum mucra...@comcast.net wrote: The CDC website can tell you exactly what to do with a CJD case and it will surprise you. Pam Marcum UAMS - Original Message - From: Kimberly K Marshall kkmarsh...@anthc.org To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 24, 2011 4:06:53 PM Subject: [Histonet] cdj Hello everyone We recently recieved a case possible positive for CJD. In researching this we have found that they now say Formalin is BAD. As long as I have been a Histo tech it seems the rules were Formalin then Formic acid. But seems there are some studies saying this is no longer enough. Is there anyone out there that has changed and if so What are you doing now??? Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Original Message - From: Kimberly K Marshall kkmarsh...@anthc.org To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 24, 2011 4:06:53 PM Subject: [Histonet] cdj Hello everyone We recently recieved a case possible positive for CJD. In researching this we have found that they now say Formalin is BAD. As long as I have been a Histo tech it seems the rules were Formalin then Formic acid. But seems there are some studies saying this is no longer enough. Is there anyone out there that has changed and if so What are you doing now??? Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC Equipment
In regards to the Ventana Ultra - if you are running PIN-4 or ISH simultaneously on this instrument, the kits/ab's/probes they require take up a lot of space on the reagent carousel which limits the number of antibodies you can put on and as runs can go some where around 6-hours you could find yourself limited in what you can run without careful planning. I'm a long time user and believer in Ventana, but BondMax and Intellipath do have my loyalties as well. It depends on the needs of your lab (volume/work flow, available lab space and antibody library size), the staffing you have available and their abilities with IHC/ISH. I've done this before and the only way to really know if an instrument will work in your lab is to demo them and connect with other users of the instrument. Vikki On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin gvdob...@ihis.org wrote: The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Sheila Fonner sfon...@labpath.com 2/25/2011 10:20 AM Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC Equipment
Sorry if I confused anyone. It may have been FISH that we couldn't do on the Leica instrument. There was some reason that the docs chose to go with the Ventana. Anyway, I'm sure they are both fine instruments! Sheila From: Greg Dobbin [mailto:gvdob...@ihis.org] Sent: Friday, February 25, 2011 9:31 AM To: Sheila Fonner; histonet@lists.utsouthwestern.edu; 'Cynthia Pyse' Subject: RE: [Histonet] IHC Equipment The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Sheila Fonner sfon...@labpath.com 2/25/2011 10:20 AM Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Telepathology
Dear Histonet, Thank you for your responses to my email this past week! If you have any additional thoughts about telepathology or digital pathology, please email me or call me directly. Have a nice weekend! Andrew Andrew Byrnes VP Sales and Marketing AccelPath, LLC M: 732-312-8008 www.AccelPath.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] procedural safeguards when accessioning identical sources
Carol We mark all our prostates with dye for ease when embedding. For prostates that need to be accessioned in order we alternate the color of the dyes, hematoxylin, safranin, eosin. When grossing, the color of the dye used is in the dictation. Hope this helps. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, February 24, 2011 3:48 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] procedural safeguards when accessioning identical sources Our lab has the policy of not accessioning back to back specimens of the same source. This helps us to ensure if there is a mix up the pathologist can tell when reading the case. For example if the specimen source is a skin and they have an endocervical they would know. We are soon to be getting a higher volume of prostates and will have to accession them together. What kind of procedural safeguards do you have in place when working with a high volume of identical sources? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC Equipment
Vikki, You are absolutely right! We do all of our ISH as overnight runs to avoid the problem of space constraint with the detection kits, etc. I agree that all of the instruments have their good and bad points, and it really depends on the lab and what kind of specimens/volume you have. Didn't mean to offend anyone. I REALLY was just trying to help and give my opinion based on my own personal use. Sheila From: Victoria Baker [mailto:bakevicto...@gmail.com] Sent: Friday, February 25, 2011 10:18 AM To: Greg Dobbin Cc: Sheila Fonner; histonet@lists.utsouthwestern.edu; Cynthia Pyse Subject: Re: [Histonet] IHC Equipment In regards to the Ventana Ultra - if you are running PIN-4 or ISH simultaneously on this instrument, the kits/ab's/probes they require take up a lot of space on the reagent carousel which limits the number of antibodies you can put on and as runs can go some where around 6-hours you could find yourself limited in what you can run without careful planning. I'm a long time user and believer in Ventana, but BondMax and Intellipath do have my loyalties as well. It depends on the needs of your lab (volume/work flow, available lab space and antibody library size), the staffing you have available and their abilities with IHC/ISH. I've done this before and the only way to really know if an instrument will work in your lab is to demo them and connect with other users of the instrument. Vikki On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin gvdob...@ihis.org wrote: The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Sheila Fonner sfon...@labpath.com 2/25/2011 10:20 AM Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may
[Histonet] Deadline extended
Hi Everyone, Good news, the hotel extended the deadline for the GSH discount rate. As long as they have rooms they will honor the $99 @ night rate that includes continental breakfast and entrance to the park. There is no deadline for registering for the meeting, but for us to have name tags ready for you, please register beforehand. Information for the meeting. The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any other states as well. Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge. The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292. Room rates start at $99 which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on the link here: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx Our theme this year is METAMORPHOSIS: Transforming Histotechs. The complete program can be downloaded from our website at this link: www.histosearch.com/gshhttp://www.histosearch.com/gshhttp://www.histosearch.com/gsh%3chttp:/www.histosearch.com/gsh then click on GSH symposium link at the bottom of the home page. There you will find the complete program with registration form on page 4. The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting. If anyone has questions, please contact me for assistance. Come TRANSFORM yourselves. Shirley Powell GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks
Happy Friday Histonetters I want to thank everyone for the information on IHC strainers. This is just what I needed. People who actually use the machines on a daily basis giving their opinions. What a great forum we have. Hope everyone has a great weekend. Currently we are receiving 6-10 inches of snow, hey, what do you except in Buffalo. Thanks again. Cindy Cindy Pyse, CLT, HT(ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Telepathology
Okay, it's Friday so I have to write that my first thought was telepathology? Is that like telekinesis? Like processing slides and coverslipping with your mind? Would this extend to sectioning to? How about pouring your much needed cup of coffee, or even making the coffee? Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Fri, Feb 25, 2011 at 10:24 AM, Andrew Byrnes a.byr...@accelpath.comwrote: Dear Histonet, Thank you for your responses to my email this past week! If you have any additional thoughts about telepathology or digital pathology, please email me or call me directly. Have a nice weekend! Andrew Andrew Byrnes VP Sales and Marketing AccelPath, LLC M: 732-312-8008 www.AccelPath.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC Equipment
Sheila It is impossible to always get everything into an e-mail when you recieve a general info request. I thought what you said was great. Have a wonderful weekend. Vikki On Fri, Feb 25, 2011 at 10:25 AM, Sheila Fonner sfon...@labpath.com wrote: Vikki, You are absolutely right! We do all of our ISH as overnight runs to avoid the problem of space constraint with the detection kits, etc. I agree that all of the instruments have their good and bad points, and it really depends on the lab and what kind of specimens/volume you have. Didn’t mean to offend anyone. I REALLY was just trying to help and give my opinion based on my own personal use. Sheila *From:* Victoria Baker [mailto:bakevicto...@gmail.com] *Sent:* Friday, February 25, 2011 10:18 AM *To:* Greg Dobbin *Cc:* Sheila Fonner; histonet@lists.utsouthwestern.edu; Cynthia Pyse *Subject:* Re: [Histonet] IHC Equipment In regards to the Ventana Ultra - if you are running PIN-4 or ISH simultaneously on this instrument, the kits/ab's/probes they require take up a lot of space on the reagent carousel which limits the number of antibodies you can put on and as runs can go some where around 6-hours you could find yourself limited in what you can run without careful planning. I'm a long time user and believer in Ventana, but BondMax and Intellipath do have my loyalties as well. It depends on the needs of your lab (volume/work flow, available lab space and antibody library size), the staffing you have available and their abilities with IHC/ISH. I've done this before and the only way to really know if an instrument will work in your lab is to demo them and connect with other users of the instrument. Vikki On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin gvdob...@ihis.org wrote: The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Sheila Fonner sfon...@labpath.com 2/25/2011 10:20 AM Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list
[Histonet] Checks and balances for specimen accessioning
Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Telepathology
It's not nice to make fun of people's typing boo-boo's, but thanks for the Friday chuckle!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, February 25, 2011 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Telepathology Okay, it's Friday so I have to write that my first thought was telepathology? Is that like telekinesis? Like processing slides and coverslipping with your mind? Would this extend to sectioning to? How about pouring your much needed cup of coffee, or even making the coffee? Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Fri, Feb 25, 2011 at 10:24 AM, Andrew Byrnes a.byr...@accelpath.comwrote: Dear Histonet, Thank you for your responses to my email this past week! If you have any additional thoughts about telepathology or digital pathology, please email me or call me directly. Have a nice weekend! Andrew Andrew Byrnes VP Sales and Marketing AccelPath, LLC M: 732-312-8008 www.AccelPath.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Checks and balances for specimen accessioning
To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Telepathology
I believe that Telepathology should be the name of what one of our pathologists wanted us to do. Cut to the small focus of tumor and stop. On Fri, Feb 25, 2011 at 8:12 AM, sgoe...@mirnarx.com wrote: It's not nice to make fun of people's typing boo-boo's, but thanks for the Friday chuckle!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, February 25, 2011 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Telepathology Okay, it's Friday so I have to write that my first thought was telepathology? Is that like telekinesis? Like processing slides and coverslipping with your mind? Would this extend to sectioning to? How about pouring your much needed cup of coffee, or even making the coffee? Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Fri, Feb 25, 2011 at 10:24 AM, Andrew Byrnes a.byr...@accelpath.comwrote: Dear Histonet, Thank you for your responses to my email this past week! If you have any additional thoughts about telepathology or digital pathology, please email me or call me directly. Have a nice weekend! Andrew Andrew Byrnes VP Sales and Marketing AccelPath, LLC M: 732-312-8008 www.AccelPath.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Thanks
So cruel! On Fri, Feb 25, 2011 at 8:13 AM, sgoe...@mirnarx.com wrote: It's sunshine and 75 here in Texas...nanny nanny...I do love my state's weather!! We had shorts on yesterday =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, February 25, 2011 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thanks Happy Friday Histonetters I want to thank everyone for the information on IHC strainers. This is just what I needed. People who actually use the machines on a daily basis giving their opinions. What a great forum we have. Hope everyone has a great weekend. Currently we are receiving 6-10 inches of snow, hey, what do you except in Buffalo. Thanks again. Cindy Cindy Pyse, CLT, HT(ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Thanks
Texas Rocks! 2 years and 2 months until I'm home. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: sgoe...@mirnarx.com sgoe...@mirnarx.com To: cp...@x-celllab.com; histonet@lists.utsouthwestern.edu Sent: Fri, February 25, 2011 10:13:19 AM Subject: RE: [Histonet] Thanks It's sunshine and 75 here in Texas...nanny nanny...I do love my state's weather!! We had shorts on yesterday =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, February 25, 2011 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thanks Happy Friday Histonetters I want to thank everyone for the information on IHC strainers. This is just what I needed. People who actually use the machines on a daily basis giving their opinions. What a great forum we have. Hope everyone has a great weekend. Currently we are receiving 6-10 inches of snow, hey, what do you except in Buffalo. Thanks again. Cindy Cindy Pyse, CLT, HT(ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Checks and balances for specimen accessioning
Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets hard-wired after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 Michael Mihalik m...@pathview.com 2/25/2011 10:22 AM To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] kits for LCM archival FFPE tissue
Hi all, I am about to perform PCR on archival FFPE tissue after LCM. My LCM system is from Arcturus and my tissue is on membraned glass slides. My questions are: 1. What Kit to use to get the highest yields and quality RNA? 2. Can anyone who has been doing it for the past 2-3 years suggest me a good amplification and purification kit for FFPE tissue? 3. Can you please send me the serial numbers of the kits? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Checks and balances for specimen accessioning
..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: Mahoney,Janice A [mailto:janice.maho...@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets hard-wired after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 Michael Mihalik m...@pathview.com 2/25/2011 10:22 AM To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
RE: [Histonet] Checks and balances for specimen accessioning
Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person preforming the task of accessioning to diligently check sample information is correct on both the container and the requisition. We too have implemented LEAN concepts throughout our AP flow and have very strict SOPs to address how to handle external incidents of this nature. We do not process until the sample has been corrected by the sender. For us; LEAN concepts/management is the preferred tool and then instrumentation selection is layered over our LEAN environment that interfaces with our LIS. Regards sue -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: February 25, 2011 9:47 AM To: 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: Mahoney,Janice A [mailto:janice.maho...@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets hard-wired after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 Michael Mihalik m...@pathview.com 2/25/2011 10:22 AM To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases
RE: [Histonet] Checks and balances for specimen accessioning
Same here Sue, thanks for the reply. Jan -Original Message- From: Finley, Sue [PH] [mailto:sfin...@providencehealth.bc.ca] Sent: Friday, February 25, 2011 12:08 PM To: 'Michael Mihalik'; Mahoney,Janice A; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person preforming the task of accessioning to diligently check sample information is correct on both the container and the requisition. We too have implemented LEAN concepts throughout our AP flow and have very strict SOPs to address how to handle external incidents of this nature. We do not process until the sample has been corrected by the sender. For us; LEAN concepts/management is the preferred tool and then instrumentation selection is layered over our LEAN environment that interfaces with our LIS. Regards sue -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: February 25, 2011 9:47 AM To: 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: Mahoney,Janice A [mailto:janice.maho...@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets hard-wired after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 Michael Mihalik m...@pathview.com 2/25/2011 10:22 AM To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Checks and balances for specimen accessioning Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure
RE: [Histonet] Checks and balances for specimen accessioning
One quick clarification: If the OR or whomever collects the specimen would put a barcoded label on the specimen container at collection time, all of this would be avoided. That just seems to be a difficult thing for those departments to do. I know that it's not a technical issue. It's something more than that. ..but it can be done and in what appears to be a small percentage of facilities, is indeed done. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: Finley, Sue [PH] [mailto:sfin...@providencehealth.bc.ca] Sent: Friday, February 25, 2011 10:08 AM To: 'Michael Mihalik'; 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person preforming the task of accessioning to diligently check sample information is correct on both the container and the requisition. We too have implemented LEAN concepts throughout our AP flow and have very strict SOPs to address how to handle external incidents of this nature. We do not process until the sample has been corrected by the sender. For us; LEAN concepts/management is the preferred tool and then instrumentation selection is layered over our LEAN environment that interfaces with our LIS. Regards sue -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: February 25, 2011 9:47 AM To: 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: Mahoney,Janice A [mailto:janice.maho...@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets hard-wired after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 Michael Mihalik m...@pathview.com 2/25/2011 10:22 AM To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, February 25, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu Subject:
RE: [Histonet] Checks and balances for specimen accessioning
But you have to hope the whom ever is putting the barcode label on uses the correct patient barcode. That has been the problem that I have seen. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Friday, February 25, 2011 12:49 PM To: 'Finley, Sue [PH]'; 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning One quick clarification: If the OR or whomever collects the specimen would put a barcoded label on the specimen container at collection time, all of this would be avoided. That just seems to be a difficult thing for those departments to do. I know that it's not a technical issue. It's something more than that. ..but it can be done and in what appears to be a small percentage of facilities, is indeed done. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: Finley, Sue [PH] [mailto:sfin...@providencehealth.bc.ca] Sent: Friday, February 25, 2011 10:08 AM To: 'Michael Mihalik'; 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person preforming the task of accessioning to diligently check sample information is correct on both the container and the requisition. We too have implemented LEAN concepts throughout our AP flow and have very strict SOPs to address how to handle external incidents of this nature. We do not process until the sample has been corrected by the sender. For us; LEAN concepts/management is the preferred tool and then instrumentation selection is layered over our LEAN environment that interfaces with our LIS. Regards sue -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: February 25, 2011 9:47 AM To: 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: Mahoney,Janice A [mailto:janice.maho...@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets hard-wired after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438
RE: [Histonet] Checks and balances for specimen accessioning
That's my goal in the next year! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Friday, February 25, 2011 1:49 PM To: 'Finley, Sue [PH]'; 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning One quick clarification: If the OR or whomever collects the specimen would put a barcoded label on the specimen container at collection time, all of this would be avoided. That just seems to be a difficult thing for those departments to do. I know that it's not a technical issue. It's something more than that. ..but it can be done and in what appears to be a small percentage of facilities, is indeed done. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: Finley, Sue [PH] [mailto:sfin...@providencehealth.bc.ca] Sent: Friday, February 25, 2011 10:08 AM To: 'Michael Mihalik'; 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person preforming the task of accessioning to diligently check sample information is correct on both the container and the requisition. We too have implemented LEAN concepts throughout our AP flow and have very strict SOPs to address how to handle external incidents of this nature. We do not process until the sample has been corrected by the sender. For us; LEAN concepts/management is the preferred tool and then instrumentation selection is layered over our LEAN environment that interfaces with our LIS. Regards sue -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: February 25, 2011 9:47 AM To: 'Mahoney,Janice A'; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Checks and balances for specimen accessioning ..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which material from outside the lab comes into the lab. It's the 'interface' or the place where the river water meets the ocean water if you follow my analogy. We have a lot more control over material once it enters the lab. It gets frustrating to spend so much effort and monies on improving practices inside the lab, only to be subject to issues outside of the lab. Yes, you can check for them by manual processes (name verification), but in large volume environments, that's gets tougher. I just get frustrated that so many specimen still come down to the lab without a patient id/order #/something barcode, so that I can ensure positive identification. This is important stuff. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: Mahoney,Janice A [mailto:janice.maho...@alegent.org] Sent: Friday, February 25, 2011 9:12 AM To: 'Nita Searcy'; Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets hard-wired after a while. The inspection of the requisition against the container is one of the steps in the process. Errors can be virtually eliminated using this practice. There is also the wonderful innovation of using bar coding to assure things match. Check out the Vantage system by Ventana. I highly recommend it for eliminating the kind of mistakes you point out. Jan Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Friday, February 25, 2011 10:26 AM To: Allison D' 'Scott; histonet@lists.utsouthwestern.edu; Michael Mihalik Subject: RE: [Histonet] Checks and balances for specimen accessioning You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager,
RE: [Histonet] cdj
This is what I found at the CDC website: Table 9. Tissue Preparation for Human CJD and Related Diseases 1. Histology technicians wear gloves, apron, laboratory coat, and face protection. 2. Adequate fixation of small tissue samples (e.g., biopsies) from a patient with suspected prion disease can be followed by post-fixation in 96% absolute formic acid for 30 minutes, followed by 45 hours in fresh 10% formalin. 3. Liquid waste is collected in a 4L waste bottle initially containing 600 ml 6N NaOH. 4. Gloves, embedding molds, and all handling materials are disposed s regulated medical waste. 5. Tissue cassettes are processed manually to prevent contamination of tissue processors. 6. Tissues are embedded in a disposable embedding mold. If used, forceps are decontaminated as in Table 10. 7. In preparing sections, gloves are worn, section waste is collected and disposed in a regulated medical waste receptacle. The knife stage is wiped with 2N NaOH, and the knife used is discarded immediately in a “regulated medical waste sharps” receptacle. Slides are labeled with “CJD Precautions.” The sectioned block is sealed with paraffin. 8. Routine staining: a. slides are processed by hand; b. reagents are prepared in 100 ml disposable specimen cups; c. after placing the cover slip on, slides are decontaminated by soaking them for 1 hour in 2N NaOH; d. slides are labeled as “Infectious-CJD.” 9. Other suggestions: a. disposable specimen cups or slide mailers may be used for reagents; b. slides for immunocytochemistry may be processed in disposable Petri dishes; c. equipment is decontaminated as described above or disposed as regulated medical waste. Handling and processing of tissues from patients with suspected prion disease The special characteristics of work with prions require particular attention to the facilities, equipment, policies, and procedures involved.10 The related considerations outlined in Table 9 should be incorporated into the laboratory’s risk management for this work. 288 Biosafety in Microbiological and Biomedical Laboratories Table 10. Prion Inactivation Methods for Reusable Instruments and Surfaces 1. Immerse in 1 N NaOH, heat in a gravity displacement autoclave at 121ºC for 30 minutes. Clean and sterilize by conventional means. 2. Immerse in 1 N NaOH or sodium hypochlorite (20,000 ppm) for 1 hours. Transfer into water and autoclave (gravity displacement) at 121ºC for 1 hour. Clean and sterilize by conventional means. 3. Immerse in 1N NaOH or sodium hypochlorite (20,000) for 1 hour. Rinse instruments with water, transfer to open pan and autoclave at 121ºC (gravity displacement) or 134ºC (porous load) for 1 hour. Clean and sterilize by conventional means. 4. Surfaces or heat-sensitive instruments can be treated with 2N NaOH or sodium hypochlorite (20,000 ppm) for 1 hour. Ensure surfaces remain wet for entire period, then rinse well with water. Before chemical treatment, it is strongly recommended that gross contamination of surfaces be reduced because the presence of excess organic material will reduce the strength of either NaOH or sodium hypochlorite solutions. 5. Environ LpH (EPA Reg. No. 1043-118) may be used on washable, hard, non-porous surfaces (such as floors, tables, equipment, and counters), items (such as non-disposable instruments, sharps, and sharp containers), and/or laboratory waste solutions (such as formalin or other liquids). This product is currently being used under FIFRA Section 18 exemptions in a number of states. Users should consult with the state environmental protection office prior to use. (Adapted from www.cdc.gov 11,12) Working Solutions 1 N NaOH equals 40 grams of NaOH per liter of water. Solution should be prepared daily. A stock solution of 10 N NaOH can be prepared and fresh 1:10 dilutions (1 part 10 N NaOH plus 9 parts water) used daily. 20,000 ppm sodium hypochlorite equals a 2% solution. Most commercial household bleach contains 5.25% sodium hypochlorite, therefore, make a 1:2.5 dilution (1 part 5.25% bleach plus 1.5 parts water) to produce a 20,000 ppm solution. This ratio can also be stated as two parts 5.25% bleach to three parts water. Working solutions should be prepared daily. CAUTION: Above solutions are corrosive and require suitable personal protective equipment and proper secondary containment. These strong corrosive solutions require careful disposal in accordance with local regulations. Precautions in using NaOH or sodium hypochlorite solutions in autoclaves: NaOH spills or gas may damage the autoclave if proper containers are not used. The use of containers with a rim and lid designed for condensation to collect and drip back into the pan is recommended. Persons who use this procedure should be cautious in handling hot NaOH solution (post-autoclave) and in avoiding potential exposure to gaseous NaOH; exercise caution during all sterilization steps; and allow the autoclave, instruments, and solutions to
Re: [Histonet] Checks and balances for specimen accessioning
Do you recall the source of pick up for the specimen? If it is picked up by Histology either in the OR or a lab site the pick up person should be checking the name on requisition, container and label in the book or ledger prior to initialing and dating. Also the person bringing the specimen to these sites has to initial and date when they put the pt. label in the ledger. I would go back to the source - as a starting point. Once the specimen is received into the lab, the person accessioning is checking the container against the requisition. Once a patient is registered either in the hospital or at the site they are assigned a medical record number or another unique identifier. When you accession this patient by one of these identifiers the descrepancy should (I stress should) show up and be caught. Another check is to see if the specimen on the container matches what is on the requisition. Something which should be done from pick up point. It varies by site how the specimen is dictated in regards to specimen ID. Some read from the bottle while looking at the requisition and dictate from that, but I've found it isn't always the rule. The original label (source label) has to be clearly visible and not covered with another label at any time in the process. If you are using a barcode label in the Histology lab the barcode needs to be placed way from the source label. The LEAN process does indeed standardize a lot of the work process, but it is also based on the lab volume, work flow which is individual to each lab. Also the use of computer systems and how fully they are utilized within each lab. I've worked in several labs that are LEAN and they still had issues with specimen handling/verification for different reasons. In your situation you would have to follow the paper trail at this point, but it would be a very strong reason to work towards getting a unified system in place that tracks specimens from the source to final sign out. I know that there are companies out there that do this one is PathCentral they provide software and support. Have a great weekend. Vikki On Fri, Feb 25, 2011 at 11:00 AM, Scott, Allison D allison_sc...@hchd.tmc.edu wrote: Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch it either. They usually peel back the copath label and look at the name on the label that came from the procedure area. In my case the resident did not do this. It was not until the pathologist saw a discrepancy in the age on the requisition and what was written in the pertinent history, that it was determined that it had been mislabeled from the beginning. I did a incident report and the area was cited. Besides making sure that who ever is accesioning cases checks that the names match, what else can be done? Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Poor nuclear artifact
Happy Friday Histo people!!! I need some opinions on a processing issue I cant seem to fix. I run a short processing run each night for my small biopsies. With two processors, I rotate so the machine with the newest reagents is the one we run as the biopsy machine. For several weeks now my Pathologist have had issues with one or two blocks a day or one day it was one piece of tissue of many in a GI biopsy block,(only one piece of like 5 were affected the rest were fine) that they are saying has poor nuclear artifact . Most days it is only one block and has happend only once with tissue from the long run. This happens no matter a weekend or over night, fresh reagents or not. I have biopsies come from all over Alaska and in talking with them as well as checking the containers they are submitting in 10% NBF, The stainer is rotated daily so its not a Xylene not clearing the slide problem. I know sections can be thicker in a ribbon but the Pathologist dont seem to think that is the problem either. I have run out of things it could be and could really use some advise. Thanks and have a great weekend ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Poor nuclear artifact
My problem in understanding your difficulty is the definition: what is poor nuclear artifact? No detail? Weak staining? Strong staining? Empty nuclus? The cause of the problem will vary depending on its definition. Unless you are more specific I think it will be very difficult in trying to help you. Please be more specific René J. --- On Fri, 2/25/11, Marshall, Kimberly K kkmarsh...@anthc.org wrote: From: Marshall, Kimberly K kkmarsh...@anthc.org Subject: [Histonet] Poor nuclear artifact To: histonet@lists.utsouthwestern.edu Date: Friday, February 25, 2011, 3:34 PM Happy Friday Histo people!!! I need some opinions on a processing issue I cant seem to fix. I run a short processing run each night for my small biopsies. With two processors, I rotate so the machine with the newest reagents is the one we run as the biopsy machine. For several weeks now my Pathologist have had issues with one or two blocks a day or one day it was one piece of tissue of many in a GI biopsy block,(only one piece of like 5 were affected the rest were fine) that they are saying has poor nuclear artifact . Most days it is only one block and has happend only once with tissue from the long run. This happens no matter a weekend or over night, fresh reagents or not. I have biopsies come from all over Alaska and in talking with them as well as checking the containers they are submitting in 10% NBF, The stainer is rotated daily so its not a Xylene not clearing the slide problem. I know sections can be thicker in a ribbon but the Pathologist dont seem to think that is the problem either. I have run out of things it could be and could really use some advise. Thanks and have a great weekend ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
