[Histonet] How to Unsubscribe
It's a do-it-yourself. - Scroll to bottom of any Histonet email. - Click on the link that has the word lists in it. - Scroll to the bottom of that page, where there is information on how to unsubscribe. - Follow directions. Peggy Wenki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] UCP1 Antibody
Hi Histonetters, I was hoping those of you who may have worked with UCP1 antibody might be willing to share how you optimized this antibody. I am using UCP1 antibody (ab10983) from Abcam. I have followed the datasheet and have used both protease and proteinase k and at varying times with no results. I am using brown fat from a wild type mouse and this is the control tissue that should work. I am running it overnight in a 4 ° to see if that might help since the Post Doc said that their western blot worked better that way. Any help or ideas how to proceed would be appreciated. Thanks in advance! Kind Regards! John J Shelley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC validation
Hematoxylin should not affect the immunoreactivity (since counterstaining occurs following the IHC reaction), so no, changing the hematoxylin would not require a revalidation. Just make sure you are not obscuring the immunostain with an overly-dark counterstain. The point is to provide contrast between the DAB (brown) or fast red chromagen and to offer the reader a sense of context of the surrounding morphology of the tissue being stained. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 10 Date: Tue, 19 Apr 2011 14:04:32 -0500 From: Ring, Mary L mary.l.r...@healthpartners.com Subject: [Histonet] IHC validation To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 2594d39b69223047b6e2f4adf1e62db9010ffb487...@hpemx3.healthpartners.int Content-Type: text/plain; charset=iso-8859-1 Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains? Thanks for your help! Mary Ring, HT, QIHC Regions Hospital, St Paul, Mn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cyto prep
Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] processing times for skin specimens
Hello everyone! What is the shortest cycle you may be using for processing skin specimens? Can skin biopsies, shaves, and punches be processed well on a short run on the VIP 6? I need to find a successful protocol if possible to run skins on a 6 hour process, if possible. Thank you in advance for any input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC menu
Would anyone be willing to share their IHC menu with us? I want to see how we compare with other top-class facilities. Obviously I am more interested in pediatric facilities, but we are constantly evaluating antibodies for research studies, and what may have applications in pediatric pathology from the adult world. I'd be happy to send our menu on return Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cyto prep
We have lab assistants running thin preps. Our cytotechs screen and do HPV. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From: Cynthia Pyse cp...@x-celllab.com To: Histonet histonet@lists.utsouthwestern.edu Sent: Thu, April 21, 2011 9:51:35 AM Subject: [Histonet] cyto prep Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unsubscribe
Jupiter's thunder two people in a row? really?!!?!? LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT YOU SUBSCRIBED ON. Both of you are officially banned from the internet. Forever. Don't use it again. And don't EVER join a mailing list. EVER. Emily, who would love to unsubscribe you so I didn't have to get your ridiculous emails about how you can't do it your damn self. A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cyto prep
We no longer do Gyn, but when we did we had lab assistants do the prep. Have lab asst for non-Gyn prep currently. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, April 21, 2011 09:52 To: 'Histonet' Subject: [Histonet] cyto prep Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unsubscribe
**Listserv master: Some people are reporting problems with managing their subscription as they have submitted their unsubscription multiple times in the prescribed manner and are still getting Histonet emails. This was reported to me by one such (failed) unsubscriber.** --On Thursday, April 21, 2011 10:25 AM -0400 Emily Sours talulahg...@gmail.com wrote: Jupiter's thunder two people in a row? really?!!?!? LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT YOU SUBSCRIBED ON. Both of you are officially banned from the internet. Forever. Don't use it again. And don't EVER join a mailing list. EVER. Emily, who would love to unsubscribe you so I didn't have to get your ridiculous emails about how you can't do it your damn self. A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6118 Fx: (716) 829-2665 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] eosin bleeding on frozen sections
Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid HE stain on frozen sections. Any advice would be greatly appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Neutralizing Formalin
Is anyone out there in Histoland Neutralizing their formalin before discarding it down the drain? We are looking into doing this because of our water authorities and we are finding that it is very costly. Has anyone found a cost efficient way to do this? I would need to know what supplier you are purchasing it from and the name of the product. Thank you in advance!! Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] eosin bleeding on frozen sections
This happened to me a couple weeks ago. You got water on the slide after the eosin staining. Just run it back through alcohols, and rinse for about five minutes. Change out the alcohols, and re-stain. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christi Cosby Sent: Thursday, April 21, 2011 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin bleeding on frozen sections Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid HE stain on frozen sections. Any advice would be greatly appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hi from Southern Cali, We neutralize our formalin with a product called Neutralex. It comes in powder form and after 30 mins we can dump it down the drain. We also keep records for QC purposes. Product is purchased from a company called American MasterTech. Hope this gives you a starting place. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cyto prep
Hi Cindy- We have cyto prep/lab asst. position that performs pap and non-gyn preparation. Cytotechs screen and HPVs are sent out. Nancy Schmitt HT, MLT(ASCP) Dubuque, IA Date: Thu, 21 Apr 2011 09:51:35 -0400 From: Cynthia Pyse cp...@x-celllab.com Subject: [Histonet] cyto prep To: 'Histonet' histonet@lists.utsouthwestern.edu Message-ID: 001501cc002b$3a929360$afb7ba20$@com Content-Type: text/plain; charset=us-ascii Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cp...@x-celllab.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] eosin bleeding on frozen sections
Try dehydrating longer. If there is water left on the slide, the eosin will bleed. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christi Cosby Sent: Thursday, April 21, 2011 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin bleeding on frozen sections Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid HE stain on frozen sections. Any advice would be greatly appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] decalcification of articular cartilage
We do a lot of IHC staining on bone and cartilage samples and we use 10% formic acid for decalcification for immunohistochemistry. I'm afraid you may not be able to turnaround this in a week. The tissue needs to be adequately fixed prior to decalcification, and properly decaled. I would trim the tissue to about 4 mm thick if you can on a saw you might be able to turn this around in your time frame, but I have learned from experience if you rush decalcification you have a greater chance of running into problems. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz Sent: Wednesday, April 20, 2011 8:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of articular cartilage Dear Members, We're planning to study sheep knee articular cartilage with histochemistry and immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde at the moment. We need an effective, quick and cartilage tissue protective decalcification method. We'll perform only light microscopic examination in this study and we have to complete the study within one week. In these circumstances what are your advices? Thanks in advance. Dr. Necat Yilmaz University of Mersin School of Medicine __ ESET NOD32 Antivirus Akıllı Güvenlik tarafından sağlanan bilgiler, virüs imza veritabanı sürümü: 6057 (20110420) __ İleti ESET NOD32 Antivirus Akıllı Güvenlik tarafından denetlendi. http://www.nod32.com.tr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] decalcification of articular cartilage
You could always get formical which fixes and decals at the same time. This would eliminate the waiting for fixation. Although I do usually let it fix in straight formalin overnight first. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, April 21, 2011 12:59 PM To: Nejat Yilmaz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decalcification of articular cartilage We do a lot of IHC staining on bone and cartilage samples and we use 10% formic acid for decalcification for immunohistochemistry. I'm afraid you may not be able to turnaround this in a week. The tissue needs to be adequately fixed prior to decalcification, and properly decaled. I would trim the tissue to about 4 mm thick if you can on a saw you might be able to turn this around in your time frame, but I have learned from experience if you rush decalcification you have a greater chance of running into problems. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz Sent: Wednesday, April 20, 2011 8:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of articular cartilage Dear Members, We're planning to study sheep knee articular cartilage with histochemistry and immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde at the moment. We need an effective, quick and cartilage tissue protective decalcification method. We'll perform only light microscopic examination in this study and we have to complete the study within one week. In these circumstances what are your advices? Thanks in advance. Dr. Necat Yilmaz University of Mersin School of Medicine __ ESET NOD32 Antivirus Akıllı Güvenlik tarafından sağlanan bilgiler, virüs imza veritabanı sürümü: 6057 (20110420) __ İleti ESET NOD32 Antivirus Akıllı Güvenlik tarafından denetlendi. http://www.nod32.com.tr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] question on Leica ST4020 stainer users
Hello, Does anyone use the Leica St4020 stainer? If so, how do you have the stainer set up and how many slides do you run on the stainer before you change reagents? Which reagents do you change and which do you not? Thanks, Trini ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
We are currently in need of some ergonomic chairs for our embedding and microtomy stations. Does anyone have any informatio to share in reguard to chairs? __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How to keep paraffin sections from sticking to blade/blade holder.
While sectioning paraffin embedded tissue on a rotary microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise for me. Thanks in advance for your suggestions . Bret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Yes - make sure they're long enough in the SEAT! Mine roll around the lab like little 5-wheeled rockets, but cut off the circulation to my thighs (is that something I can say on television?). And no arms on the chairs! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processing times for skin specimens
Carol We are a research lab but we have always processed our skin samples on longer processing cycles, even the punch biopsy samples. The shaves might be different, if I wanted to cut down I would start with how you normally process them and then cut down at 5 minute increments to start and see how that goes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, April 21, 2011 8:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] processing times for skin specimens Hello everyone! What is the shortest cycle you may be using for processing skin specimens? Can skin biopsies, shaves, and punches be processed well on a short run on the VIP 6? I need to find a successful protocol if possible to run skins on a 6 hour process, if possible. Thank you in advance for any input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How to keep paraffin sections from sticking to blade/blade holder.
1- change cutting angle 2- keep the blade holder clean (without paraffin) 3- make sure the blade is not blunt René J. --- On Thu, 4/21/11, Clough, Bret clo...@medicine.tamhsc.edu wrote: From: Clough, Bret clo...@medicine.tamhsc.edu Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder. To: Histonet list serv. histonet@lists.utsouthwestern.edu Date: Thursday, April 21, 2011, 3:07 PM While sectioning paraffin embedded tissue on a rotary microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise for me. Thanks in advance for your suggestions . Bret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How to keep paraffin sections from sticking to blade/blade holder.
Keep everything cold, cold, cold. The block, the blade, the blade holder. Cold spray the blade and blade holder, cool the block on ice. Warning: Use cold spray sparingly on the block, you can ruin your tissue with too much. Hullabaloo, Caneck! Caneck! Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How to keep paraffin sections from sticking to blade/blade holder.
Open a beer to the science gods. It may also help to follow the other instructions sent to the histonet list. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Thu, Apr 21, 2011 at 3:07 PM, Clough, Bret clo...@medicine.tamhsc.eduwrote: While sectioning paraffin embedded tissue on a rotary microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise for me. Thanks in advance for your suggestions . Bret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] endogenous peroxidases
Hello I know I can block endogenous peroxidases in paraffin sections before antigen retrieval or after it, but which is better? Does it make a difference? Also, since our H2O2 is 30%, do I need to account for the water in it when I'm diluting it in 100% MeOH? (I was going to use a 0.3% H2O2 in MeOH wash for 30 minutes) I'm not even sure how I would account for it, I just thought it might affect the MeOH since it wouldn't be anhydrous anymore. Emily ps put your hand to the screen for an internet high five!! it's national high five day! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] endogenous peroxidases
The endogenous peroxidase has to be blocked (quenching others call this step) only before the procedure because the idea is to avoid the interference of the peroxidase with it. René J. --- On Thu, 4/21/11, Emily Sours talulahg...@gmail.com wrote: From: Emily Sours talulahg...@gmail.com Subject: [Histonet] endogenous peroxidases To: histonet@lists.utsouthwestern.edu Date: Thursday, April 21, 2011, 4:32 PM Hello I know I can block endogenous peroxidases in paraffin sections before antigen retrieval or after it, but which is better? Does it make a difference? Also, since our H2O2 is 30%, do I need to account for the water in it when I'm diluting it in 100% MeOH? (I was going to use a 0.3% H2O2 in MeOH wash for 30 minutes) I'm not even sure how I would account for it, I just thought it might affect the MeOH since it wouldn't be anhydrous anymore. Emily ps put your hand to the screen for an internet high five!! it's national high five day! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mohs Key Performane Indicators (Recuts)
Hello, I work for a Mohs surgeon who wants to establish key performance indicators (KPIs) for the mohs techs to evaluate daily performance. Does anyone have any information of surveys or standards for the number of recuts per day or per block as a measure for quality Mohs sectioning? She's working with a standard that 4 or less recuts in a day (average 30 to 50 blocks per day) is quality work. Has anyone come up with standards in this regard for Mohs sectioning? Thanks in advance, Martino Tanner “Life can either be accepted or changed. If it is not accepted, it must be changed. If it cannot be changed, then it must be accepted.” ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Input needed
What’s the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How to keep paraffin sections from sticking to blade/blade holder.
Another culprit is static. Moisture helps to keep the static down. Clough, Bret clo...@medicine.tamhsc.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 04/21/2011 12:11 PM To Histonet list serv. histonet@lists.utsouthwestern.edu cc Subject [Histonet] How to keep paraffin sections from sticking to blade/blade holder. While sectioning paraffin embedded tissue on a rotary microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise for me. Thanks in advance for your suggestions . Bret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Input needed
I have had graduates from my program that are mature. They have not had problems getting jobs, or advancing once hired. That is true for either gender. A good tech is a good tech. Jennifer MacDonald Mt. San Antonio College Mary mhunt...@earthlink.net Sent by: histonet-boun...@lists.utsouthwestern.edu 04/21/2011 03:08 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Input needed What’s the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] processing times for skin specimens
90 minutes for up to 2mm thickness using K3000 or VIP5. I do not have a VIP6 Steve A. McClain, MD McClain Laboratories, LLC 45 Manor Road Smithtown, NY 11787 631 361-4000 631 361-4037fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, April 21, 2011 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 89, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Cassette labeler (Martin, Gary) 2. 'Deplasticizing' before staining? (Mahesh Polavarapu) 3. Re: Request help on finding a part for Leitz 1512 (Henry Chidgey) 4. RELIA Histology Job Alert. Excellent opportunity on Long Island Day shift NY Lic required. (Pam Barker) 5. RE: Re: Request help on finding a part for Leitz 1512 (Weems, Joyce) 6. Re: Re: Request help on finding a part for Leitz 1512 (Grantham, Andrea L - (algranth)) 7. Part time Histo Tech position in Phoenix AZ (jco...@yahoo.com) 8. Unsubscribe (Tate Maria) 9. Re: Unsubscribe (Mollie Hannon) 10. Graduating in June from histo program (Teresa Moore) 11. RE: whole retinal staining (Abbey Mortimer) 12. How to Unsubscribe (Lee Peggy Wenk) 13. trichrome/ frozen cardiac tissue (Molinari, Betsy) 14. UCP1 Antibody (John Shelley) 15. IHC validation (Troutman, Kenneth A) 16. cyto prep (Cynthia Pyse) 17. processing times for skin specimens (Carol Bryant) 18. IHC menu (Houston, Ronald) 19. Re: cyto prep (Sheila Haas) 20. Re: Unsubscribe (Emily Sours) 21. RE: cyto prep (Weems, Joyce) 22. Re: Unsubscribe (Merced Leiker) 23. eosin bleeding on frozen sections (Christi Cosby) 24. Neutralizing Formalin (Evans, Andria B) 25. RE: eosin bleeding on frozen sections (sgoe...@mirnarx.com) 26. (no subject) (SHANE NELSON) -- Message: 1 Date: Wed, 20 Apr 2011 10:12:34 -0700 From: Martin, Gary gmar...@marshallmedical.org Subject: [Histonet] Cassette labeler To: histonet@lists.utsouthwestern.edu Message-ID: 6ED9D4252F278841A0593D3D788AF24C0D480110@mailsvr.MARSHMED.local Content-Type: text/plain; charset=us-ascii We have just received a Lamb Micro Writer (model # E22.01MWI) single shoe. The problem is that we did not receive an operator manual. Does anyone know where I can get a manual? -- Message: 2 Date: Wed, 20 Apr 2011 12:24:55 -0500 From: Mahesh Polavarapu polavarapu.mah...@gmail.com Subject: [Histonet] 'Deplasticizing' before staining? To: histonet histonet@lists.utsouthwestern.edu Message-ID: BANLkTim6mC2iuyvCLeX5G=9fwspcjrq...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi, I have 50uM thick sections of soft tissue embedded in MMA (cold cured Technovit 9100). Do these need to be 'deplasticized' before they will take up stain? Thanks in advance! - Mahesh -- Message: 3 Date: Wed, 20 Apr 2011 12:31:10 -0500 From: Henry Chidgey hchid...@earthlink.net Subject: [Histonet] Re: Request help on finding a part for Leitz 1512 To: histonet@lists.utsouthwestern.edu Message-ID: 016f01cbff80$bef19030$3cd4b090$@net Content-Type: text/plain; charset=us-ascii I was asked by a member to share the solution so here it is : Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA. He will probably have some or can make you some. Great guy and very knowledgeable. Thanks Joyce for giving me this informationJ Henry -- Message: 4 Date: Wed, 20 Apr 2011 13:36:45 -0400 From: Pam Barker rel...@earthlink.net Subject: [Histonet] RELIA Histology Job Alert. Excellent opportunity on Long Island Day shift NY Lic required. To: 'Histonet' histonet@lists.utsouthwestern.edu Message-ID: DCFA9EE40E204519866E4B9E75992D67@ownerf1abaad51 Content-Type: text/plain; charset=iso-8859-1 Hi Histonetters!! I have a great new opportunity that I want to tell you about. I am working with a Laboratory located on Long Island that is in need of a NY licensed histology tech. This is a dayshift full time permanent position. My client offers a very competitive salary, excellent benefits, a great place to work and they are ready to interview and hire right away. If you or anyone you know might be interested in this position please contact me. I can be reached toll
[Histonet] Re: processing times for skin specimens
We do a short run some mornings for shaves and small punches. Keep in mind that we don't work 24/7, so these have been sitting in formalin overnight. 20 min formalin 1 min distilled water 10 min 80% alcohol 10 min 90% alcohol 10 min 95% alcohol 10 min absolute alcohol 20 min absolute alcohol 10 min pro-par (xylene substitute) 10 min pro-par 20 min pro-par 10 min paraffin 10 min paraffin 10 min paraffin 10 min paraffin That comes out to be about 3 hours of time once you factor in the pump in/pump out time, and we have a VIP 5. Nathan Jentsch BS HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
