RE: [Histonet] Keeping Histo room floor clean?
I have a tool that I got from those odd ball lab supply company that carry a little of everything. Its long like a broom handle and has a scraper blade on the bottom with a removable replaceable blade. Cost like 20 bucks works great then I just sweep it up. Nicole Tatum, HT ASCP You can buy this type of thing too if you aren't the McGiver type. For instance...from American Mastertech item # CPW04200E Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Tuesday, June 14, 2011 7:50 PM To: JR R Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Keeping Histo room floor clean? We don't keep it off the floor, but do use a wide-bladed putty knife attached to a mop handle to scrape the residual wax off the floor. It woks quite nicely and doesn't remove the actual floor wax like a razor blade scrapper would. Michelle Sent from my iPhone On Jun 14, 2011, at 6:44 PM, JR R rosenfeld...@hotmail.com wrote: We keep getting a lot of paraffin on the floor of one histo room--especially around the microtome and the embedding station. Short of laying down a tarp, what do folks do keep wax off of the floor? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Decal Solutions
I have used formic acid (5%) for decal for IHC for 20 years, it is slower than HCL but much better for IHC, if you use a platform shaker to keep it moving the exchange can be pretty good for bm bx 4-6 hours is what I would start with. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Monday, June 13, 2011 8:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solutions Hi, Does anyone know of any decal solutions that are compatible with IHC, ISH, FISH other than EDTA and solutions containing HCL ? We have heard that some people use formic acid. Also, what would be the decalcification times for tissue such as a bone marrow bx using these other solutions ? Any help would be greatly appreciated... Thanks, Vicki Gauch AMCH Albany, NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Decal Solutions
We do 95% bone marrow specimens here. We have perfected our ISH and IHC using the Rapid-Cal Immuno from BBC. Most bone marrows decal in 2.5 hours (8 gauge). The decal does not diminish the iron stores either. If you have questions about my procedures feel free to contact me. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Monday, June 13, 2011 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solutions Hi, Does anyone know of any decal solutions that are compatible with IHC, ISH, FISH other than EDTA and solutions containing HCL ? We have heard that some people use formic acid. Also, what would be the decalcification times for tissue such as a bone marrow bx using these other solutions ? Any help would be greatly appreciated... Thanks, Vicki Gauch AMCH Albany, NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Retirement
The smoking thing is what I missed...I know I work in cancer research and I shouldn't smoke, but as I tell everyone...by the time I get lung cancer I will have helped to find the cure (wishful thinking and stupid excuse making I know.) Now almost everywhere I work you have to completely leave the property to smoke, and the latest talk is that soon we won't be able to even smoke on a public sidewalk! I'm sure in my lifetime I will see cigarettes become illegal and pot become legal, kinda funny I think =) Happy Monday all!! PS-Is anyone else out there going to the Innovex thing in CA this week?? I need a happy hour/go to Alcatraz tour buddy =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Sunday, June 19, 2011 7:48 PM To: Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: Re: [Histonet] Retirement And remember mouth pipeting? oops, that's the cotton... Eating and smoking in the lab was the norm. Our alcohol had the tax stamp on it! :o) Michelle On Jun 19, 2011, at 4:19 PM, Paula Sicurello pat...@gmail.com wrote: Sheesh! We used to have people smoke while working with propylene oxide. Eating in your control pigs was part of the benefit of being a graduate student to save on grocery money. Film? My TEM used glass plates. Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. Wearing closed toed shoes was for wimps, you were just fast if you dropped a steel wedge blade. We even wrote using the entire word and proper grammar, none of this acronym stuff for us. Retirement? What's that? Paula :-) On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks amosbro...@gmail.com wrote: Agarose Gels! ... Listen you whipersnapper Agarose is the easy way out. When I learned it we used to have to make up our own polyacrylamide gels. That was after having to walk to work up hill both ways in 30 feet of snow! (No nearer retirement) Crotchety Amos Message: 7 Date: Fri, 17 Jun 2011 13:24:12 -0400 From: Emily Sours talulahg...@gmail.com Subject: Re: [Histonet] Retirement To: histonet@lists.utsouthwestern.edu Message-ID: banlktinejxtxyop-byfweuxn3yw-ff3...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Retirement? I think by the time I get to that point, social security will have run out. Then again, technology will be so advanced, I can tell stories about the old days, where I logged on to the bbs by modem to post messages to my friends and typed in my own html coding. We didn't have google when I was young!! Our cameras used film! And you couldn't see how bad your pictures were until you developed that film!! There was no PCR to sequence your DNA, you ran an agarose gel and hoped for the best!! You could drink the 100% ethanol, there was no denaturing! (okay that was before my time) You could smoke in the lab while you sectioned without gloves!! (okay that was too) Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Retirement
You had COTTON in your pipettes? We used hollowed out reeds with a bit of papyrus in one end. No calibration But plenty accurate enough for histo in those days. However, there are a few things I do not miss from back in da' day. Hand-stropping a knife for an hour, only to have it nicked by a staple in the next hour. -Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Sunday, June 19, 2011 7:48 PM To: Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: Re: [Histonet] Retirement And remember mouth pipeting? oops, that's the cotton... Eating and smoking in the lab was the norm. Our alcohol had the tax stamp on it! :o) Michelle On Jun 19, 2011, at 4:19 PM, Paula Sicurello pat...@gmail.com wrote: Sheesh! We used to have people smoke while working with propylene oxide. Eating in your control pigs was part of the benefit of being a graduate student to save on grocery money. Film? My TEM used glass plates. Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. Wearing closed toed shoes was for wimps, you were just fast if you dropped a steel wedge blade. We even wrote using the entire word and proper grammar, none of this acronym stuff for us. Retirement? What's that? Paula :-) On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks amosbro...@gmail.com wrote: Agarose Gels! ... Listen you whipersnapper Agarose is the easy way out. When I learned it we used to have to make up our own polyacrylamide gels. That was after having to walk to work up hill both ways in 30 feet of snow! (No nearer retirement) Crotchety Amos Message: 7 Date: Fri, 17 Jun 2011 13:24:12 -0400 From: Emily Sours talulahg...@gmail.com Subject: Re: [Histonet] Retirement To: histonet@lists.utsouthwestern.edu Message-ID: banlktinejxtxyop-byfweuxn3yw-ff3...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Retirement? I think by the time I get to that point, social security will have run out. Then again, technology will be so advanced, I can tell stories about the old days, where I logged on to the bbs by modem to post messages to my friends and typed in my own html coding. We didn't have google when I was young!! Our cameras used film! And you couldn't see how bad your pictures were until you developed that film!! There was no PCR to sequence your DNA, you ran an agarose gel and hoped for the best!! You could drink the 100% ethanol, there was no denaturing! (okay that was before my time) You could smoke in the lab while you sectioned without gloves!! (okay that was too) Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Retirement
And that is why I never let you use my knives. Tom -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, June 20, 2011 10:31 AM To: histot...@imagesbyhopper.com; Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: RE: [Histonet] Retirement You had COTTON in your pipettes? We used hollowed out reeds with a bit of papyrus in one end. No calibration But plenty accurate enough for histo in those days. However, there are a few things I do not miss from back in da' day. Hand-stropping a knife for an hour, only to have it nicked by a staple in the next hour. -Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Sunday, June 19, 2011 7:48 PM To: Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: Re: [Histonet] Retirement And remember mouth pipeting? oops, that's the cotton... Eating and smoking in the lab was the norm. Our alcohol had the tax stamp on it! :o) Michelle On Jun 19, 2011, at 4:19 PM, Paula Sicurello pat...@gmail.com wrote: Sheesh! We used to have people smoke while working with propylene oxide. Eating in your control pigs was part of the benefit of being a graduate student to save on grocery money. Film? My TEM used glass plates. Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. Wearing closed toed shoes was for wimps, you were just fast if you dropped a steel wedge blade. We even wrote using the entire word and proper grammar, none of this acronym stuff for us. Retirement? What's that? Paula :-) On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks amosbro...@gmail.com wrote: Agarose Gels! ... Listen you whipersnapper Agarose is the easy way out. When I learned it we used to have to make up our own polyacrylamide gels. That was after having to walk to work up hill both ways in 30 feet of snow! (No nearer retirement) Crotchety Amos Message: 7 Date: Fri, 17 Jun 2011 13:24:12 -0400 From: Emily Sours talulahg...@gmail.com Subject: Re: [Histonet] Retirement To: histonet@lists.utsouthwestern.edu Message-ID: banlktinejxtxyop-byfweuxn3yw-ff3...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Retirement? I think by the time I get to that point, social security will have run out. Then again, technology will be so advanced, I can tell stories about the old days, where I logged on to the bbs by modem to post messages to my friends and typed in my own html coding. We didn't have google when I was young!! Our cameras used film! And you couldn't see how bad your pictures were until you developed that film!! There was no PCR to sequence your DNA, you ran an agarose gel and hoped for the best!! You could drink the 100% ethanol, there was no denaturing! (okay that was before my time) You could smoke in the lab while you sectioned without gloves!! (okay that was too) Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAS
Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PAS
Yes René J. From: sgoe...@mirnarx.com sgoe...@mirnarx.com To: histonet@lists.utsouthwestern.edu Sent: Monday, June 20, 2011 11:01 AM Subject: [Histonet] PAS Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PAS
Kidney is a sensitive control for PAS positive material. The basement membranes are PAS positive. It is not positive for glycogen. sgoe...@mirnarx.com Sent by: histonet-boun...@lists.utsouthwestern.edu 06/20/2011 08:03 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] PAS Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mopec MB100 Grossing Station
This is a used piece in excellent condition and half the price of new. http://media.mopec.com/media/pdf/Catalog2007smaller159.pdf This is a countertop unit. Mark Sofferman, President American ReSource Medical 324 West Englewood Avenue Teaneck, NJ 07666 P: 201.833.1550 F: 201.833.1575 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana's HER2 Dual ISH DNA Probe Cocktail
Ventana just received FDA approval for the first fully automated diagnostic assay for HER2 gene status determination in breast cancer patients. I do not have a Ventana immunostainer and from what I have been told, it is a closed system and this new probe cocktail could not be used on another stainer, such as the Dako. Is anyone out there in HistoLand using this new cocktail and am I correct that it would not work using the Dako Autostainer. A Ventana rep may contact me with this information also. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center Barre, Vermont 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ventana's HER2 Dual ISH DNA Probe Cocktail
In the past the FDA approval has been tied to running the antibody on the specific vendors' instrumentation using a predetermined protocol. This was true for Dako's EGFR that was FDA approved. Ventana antibodies can be dispensed out of their vials and used on any instrumentation, however, you may have an IVD antibody at that point. Definitely check around and see what others have to say. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne [lynne.b...@cvmc.org] Sent: Monday, June 20, 2011 1:28 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Ventana's HER2 Dual ISH DNA Probe Cocktail Ventana just received FDA approval for the first fully automated diagnostic assay for HER2 gene status determination in breast cancer patients. I do not have a Ventana immunostainer and from what I have been told, it is a closed system and this new probe cocktail could not be used on another stainer, such as the Dako. Is anyone out there in HistoLand using this new cocktail and am I correct that it would not work using the Dako Autostainer. A Ventana rep may contact me with this information also. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center Barre, Vermont 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ventana's HER2 Dual ISH DNA Probe Cocktail
If you modify any part of the test protocol from the FDA approved methodology in any way, then it is no longer an FDA approved test. Does that mean you cannot use it on your system? Absolutely not; you would have to validate it as you would any other antibody. However, you do need to be cognisant of the fact that if the FDA approved test is dependent on a patient going on a clinical trial or FDA approved treatment for a particular disease, your patient may not be accepted for that treatment regimen. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Monday, June 20, 2011 1:37 PM To: Bell, Lynne; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Ventana's HER2 Dual ISH DNA Probe Cocktail In the past the FDA approval has been tied to running the antibody on the specific vendors' instrumentation using a predetermined protocol. This was true for Dako's EGFR that was FDA approved. Ventana antibodies can be dispensed out of their vials and used on any instrumentation, however, you may have an IVD antibody at that point. Definitely check around and see what others have to say. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne [lynne.b...@cvmc.org] Sent: Monday, June 20, 2011 1:28 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Ventana's HER2 Dual ISH DNA Probe Cocktail Ventana just received FDA approval for the first fully automated diagnostic assay for HER2 gene status determination in breast cancer patients. I do not have a Ventana immunostainer and from what I have been told, it is a closed system and this new probe cocktail could not be used on another stainer, such as the Dako. Is anyone out there in HistoLand using this new cocktail and am I correct that it would not work using the Dako Autostainer. A Ventana rep may contact me with this information also. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center Barre, Vermont 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Openings in Atlanta
A dynamic private pathology lab in the Atlanta metro area has several openings for full time qualified ASCP-certified histotechs in routine histology processes including grossing, embedding, cutting, special stains and immunohistochemistry. these are full time openings. at least 3 years experience preferred Please contact me today for immediate consideration Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 br...@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog http://twitter.com/PrometheusBlog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] out of office
I will be out of the office starting 06/20/2011 and will not return until 06/23/2011. In my absence please ask for Laurie . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: PAS
Mirna, Lee Luna (Histologic 6(1):77, 1976) suggests that the best control for PAS staining, are the walls of blood vessels. They will show a bright red reaction only if the technique is working well. Glycogen may stain well even with sub-optimal reagents. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Tuesday, 21 June 2011 1:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PAS
No issue, it can be use. Basement of renal tubules blood vessel and mesangial matrix of glomeruli gives good result in PAS. Amita From: Victoria Baker bakevicto...@gmail.com To: sgoe...@mirnarx.com Cc: histonet@lists.utsouthwestern.edu Date: 20/06/11 09:08 PM Subject:Re: [Histonet] PAS Sent by:histonet-boun...@lists.utsouthwestern.edu Liver - excellent source for glycogen. I haven't used kidney-see what others say. Vikki On Jun 20, 2011 11:02 AM, sgoe...@mirnarx.com wrote: Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
