[Histonet] mouse heart trimming and sectioning_papillary muscle
Dear fellow histonetters, I would like to have your suggestions regarding trimming and sectioning of mouse heart. Our pathologists are interested in evaluating mouse ventricle papillary muscle for some lesions. I am not able to cut sections having papillary muscle in all sections.Some sections I am getting but not in all. Anybody experienced this situation and any experience to share. With regards Abi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peloris Rapid tissue processor
Dear All, Our lab is in the process of purchasing our automated tissue processor (Peloris Rapid tissue processor). I would really appreciate comments from anyone who really likes, or dislikes the processor that they are using. Thanks in advance!!! Muhammad Tahseen Histology Supervisor Deptt. Pathology Shaukat Khanum Cancer Hospital And Research Center Lahore. Pakistan Ph. +92 42 5180725-36 Ext 2369, Fax. +92 42 5180723 e-mail. Histology @t skm.org.pk ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Peloris Rapid tissue processor
Muhammad: The only thing I dislike about the Peloris technology (it is a technology in itself) is that after the dehydration with 2-propanol, the tissues are subjected to a DRY HOT evaporation of the 2-propanol in vacuum before the infiltration step with melted paraffin. That step of drying out the tissues to eliminate the 2-propanol to facilitate the infiltration is the one I do not like because the tissues are exposed to a very high gradient. If you end buying the instrument I think you should run a large series of validation tests to find out if the results you obtain with the Peloris compare with what you are used to, not referring to the sectioning quality of the blocks, but to their microscopic appearance.. Peloris was developed in Australia by VisionBioSystems and later bought by Leica Microsystems. Although there were tests published by VisionBioSystems, all referred to animal tissues, and the instrument was never independently validated. Leica did not make known (published) independent validations. I for one would never subject the tissues to a hot dry desiccation before infiltration. I hope this will help you in your decision. René J. --- On Sat, 8/13/11, tahs...@brain.net.pk tahs...@brain.net.pk wrote: From: tahs...@brain.net.pk tahs...@brain.net.pk Subject: [Histonet] Peloris Rapid tissue processor To: histonet@lists.utsouthwestern.edu Cc: histol...@skm.org.pk Date: Saturday, August 13, 2011, 6:18 AM Dear All, Our lab is in the process of purchasing our automated tissue processor (Peloris Rapid tissue processor). I would really appreciate comments from anyone who really likes, or dislikes the processor that they are using. Thanks in advance!!! Muhammad Tahseen Histology Supervisor Deptt. Pathology Shaukat Khanum Cancer Hospital And Research Center Lahore. Pakistan Ph. +92 42 5180725-36 Ext 2369, Fax. +92 42 5180723 e-mail. Histology @t skm.org.pk ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: IHC on Pig help
Treat the tissue exactly as if it was human. Sure, your anti CD68 may not work on pig but, it's worth a try. LBH? Probably LDH. If so, better to measure serum levels? Trypen blue? Prob. Trypan blue. It's a dye used for cell viability tests. So, it seems that 2 and 3 are not IHC at all. More details? Also ask for IHC help here, if you wish : http://www.immunoportal.com/ Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AUTO: Heidi Couture is out of the office. (returning 08/15/2011)
I am out of the office until 08/15/2011. I will be out of the office Thursday 8/11 Friday 8/12, returning Monday 8/15, please call 1800-225-3035 (leica customer service) with any urgent matters, or email lisa.raym...@leica-microsystems.com. Please have your account number and PO available. Thank you, have a great day! Note: This is an automated response to your message Histonet Digest, Vol 93, Issue 18 sent on 8/13/2011 11:59:41 AM. You will receive a notification for each message you send to this person while the person is away. __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: IHC on Pig help
Carl is correct... treat the tissue as if it is human... i.e., use anti-mouse IgG as your link secondary Ab for CD68 mouse primary antibody, etc. Not all CD68s cross-react with non-primates; I can let you know which clones I've tried when I get back into work on Monday. Jan Shivers IHC Section Head Veterinary Diagnostic Lab Univ. of Minnesota College of Veterinary Medicine St. Paul, MN On Aug 13 2011, Hobbs, Carl wrote: Treat the tissue exactly as if it was human. Sure, your anti CD68 may not work on pig but, it's worth a try. LBH? Probably LDH. If so, better to measure serum levels? Trypen blue? Prob. Trypan blue. It's a dye used for cell viability tests. So, it seems that 2 and 3 are not IHC at all. More details? Also ask for IHC help here, if you wish : http://www.immunoportal.com/ Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
