[Histonet] Seeking new position

2011-09-16 Thread Charles O
   Hi,
  I am a certified histotech ( with both HT (ASC) and  HTL (ASCP) Plus
QIHC (ASCP) seeking a new position. I have an extensive experience in the
general histological technics and in Immunohistochemistry. I am willing to
relocate.

Thanks,

  Charles O.
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[Histonet] HM staining

2011-09-16 Thread Carmen Maria Garcia Pascual

Good morning:

I have a question, I want to know what can I do if I get a very strong 
staining... it's reversible?

Thank you very much!
Best regards,

Carmen


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[Histonet] (no subject)

2011-09-16 Thread Teisha Robertson
a tabindex=1 title= name=uiluqffwic 
href=http://designkrby.cz/oldtemp/arccre.htm;http://designkrby.cz/oldtemp/arccre.htm/a
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[Histonet] Manual IHC staining

2011-09-16 Thread Reynolds,Donna M
We have a system we make that uses Plexiglas  boxes to create the humid 
environment and a special holder inside that holds  1-10 slides that can be 
picked up and washed all at once.
We have our new baby chamber that only holds 5 slides for the minnie jobs. Our 
chambers will hold 3 holders =30 slides, 4 holders =40 slides, and 6 holders 
=60 slides. Each slide can be treated individually so variations are almost 
endless just how adventurous you fell. \
Any one interested in these can contact me at my personal e-mail. 
cdr...@hotmail.commailto:cdr...@hotmail.com and I can supply more information.

Donna Reynolds  (HT)ASCP
281-954-5022

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[Histonet] Von Kossa vs Alizarin Red

2011-09-16 Thread Molinari, Betsy
Hi,
 Under what circumstances would you prefer one over the other? Thanks! Happy 
Friday! I hope all you convention goers ( is that even a word??) are having a 
great time!

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030-2607
832-355-6524
832-355-6812

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[Histonet] Sequenza

2011-09-16 Thread Edwards, Richard E.
According to my source at Fisher Scientific U.K, the  distributor for the  
Shandon range in the  U.K., the Sequenza   and perhaps more importantly, the  
Coverplates are still listed and available.

 Richard Edwards

 Leicester U.K.

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[Histonet] Leica Bond verses the Ventana Ultra

2011-09-16 Thread Pardue, Judith
Need to know histotechs opinion of the Leica Bond verses the Ventana
Ultra. 
 
judith_par...@memorial.org
 
Judith Pardue




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RE: [Histonet] Leica Bond verses the Ventana Ultra

2011-09-16 Thread Britton, Josette C
I love the Bond!

 

Josie Britton HT

Cheshire Medical Center 

Keene, NH

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue,
Judith
Sent: Friday, September 16, 2011 11:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond verses the Ventana Ultra

 

Need to know histotechs opinion of the Leica Bond verses the Ventana

Ultra. 

 

judith_par...@memorial.org

 

Judith Pardue

 

 

 

 

This message and accompanying documents are covered by

the Electronic Communications Privacy Act 18

U.S.C. Sections 2510-2521, and contain information

intended for the specified individual(s) only. This

information is confidential.  If you are not the intended

recipient or an agent responsible for delivering it to

the intended recipient, you are hereby notified that you

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[Histonet] RE: Leica Bond verses the Ventana Ultra

2011-09-16 Thread Horn, Hazel V
We love our Bond too.  

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith
Sent: Friday, September 16, 2011 10:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond verses the Ventana Ultra

Need to know histotechs opinion of the Leica Bond verses the Ventana
Ultra. 
 
judith_par...@memorial.org
 
Judith Pardue




This message and accompanying documents are covered by
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U.S.C. Sections 2510-2521, and contain information
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the intended recipient, you are hereby notified that you
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[Histonet] RE: Leica Bond verses the Ventana Ultra

2011-09-16 Thread Rathborne, Toni
We love our Bond, but can not compare to the Ultra for long-term use. We demo'd 
both instruments, and chose the Bond.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Friday, September 16, 2011 12:29 PM
To: 'Pardue, Judith'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Leica Bond verses the Ventana Ultra

We love our Bond too.  

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith
Sent: Friday, September 16, 2011 10:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond verses the Ventana Ultra

Need to know histotechs opinion of the Leica Bond verses the Ventana Ultra. 
 
judith_par...@memorial.org
 
Judith Pardue




This message and accompanying documents are covered by the Electronic 
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Re: [Histonet] Manual IHC staining

2011-09-16 Thread Rene J Buesa
Under separate cover I am sending a photo of our large humid chamber
René J.

--- On Fri, 9/16/11, Reynolds,Donna M dreyn...@mdanderson.org wrote:


From: Reynolds,Donna M dreyn...@mdanderson.org
Subject: [Histonet] Manual IHC staining
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Date: Friday, September 16, 2011, 9:04 AM


We have a system we make that uses Plexiglas  boxes to create the humid 
environment and a special holder inside that holds  1-10 slides that can be 
picked up and washed all at once.
We have our new baby chamber that only holds 5 slides for the minnie jobs. Our 
chambers will hold 3 holders =30 slides, 4 holders =40 slides, and 6 holders 
=60 slides. Each slide can be treated individually so variations are almost 
endless just how adventurous you fell. \
Any one interested in these can contact me at my personal e-mail. 
cdr...@hotmail.commailto:cdr...@hotmail.com and I can supply more information.

Donna Reynolds  (HT)ASCP
281-954-5022

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RE: [Histonet] RE: Leica Bond verses the Ventana Ultra

2011-09-16 Thread Ingles Claire
James Bond? Sean Connery was always my favorite. :) Happy Friday! I'm off for a 
week and a half to the northwoods. Those fish better look out, and I don't mean 
zebrafish.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rathborne, Toni
Sent: Fri 9/16/2011 11:33 AM
To: 'Horn, Hazel V'; 'Pardue, Judith'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Leica Bond verses the Ventana Ultra



We love our Bond, but can not compare to the Ultra for long-term use. We demo'd 
both instruments, and chose the Bond.




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[Histonet] Re: Von Kossa vs Alizarin Red

2011-09-16 Thread Johnson, Teri
Hi Betsy,

How timely is your question! We are (well, actually Nancy Thomas is) currently 
evaluating the two stains on some chameleon heads and we have found something 
really interesting we never considered. The chameleon has quite a bit of 
melanin pigment in places. The fact the von kossa will end up staining the bone 
black, it doesn't contrast well with the areas of pigment throughout the 
connective tissue. Otherwise, in other species I usually prefer the von kossa.

The silver technique might also work better for combined stains or combined 
histochemical and immunohistochemical staining than using the Alizarin Red. 
Just my preference.

~Teri

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[Histonet] Histology Opening in Central NJ

2011-09-16 Thread Brian- Prometheus
Reference lab located in Eatontown, NJ currently searching for a night
histotech.

 

mostly doing general histology

 

will train

 

hours roughly 9pm to 5:30am

 

Please contact me today for immediate consideration.

 

Brian Feldman

Principal

Prometheus Healthcare 

Office 301-693-9057

Fax 301-368-2478

 
http://us.mc538.mail.yahoo.com/mc/compose?to=br...@prometheushealthcare.com
 br...@prometheushealthcare.com

 http://www.prometheushealthcare.com/ www.prometheushealthcare.com

*** Stay up to date on the newest positions and healthcare trends nationwide
on Twitter!***

  http://twitter.com/PrometheusBlog http://twitter.com/PrometheusBlog

 

 

 

 

 

 

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[Histonet] Von Kossa vs Alizarin Red

2011-09-16 Thread Amos Brooks
Hi,
I do both often. You would want the VonKossa rather than Alizarin Red if
you had any plans to do any subsequent double staining. The silver isn't
going anywhere. The Alizarin Red will disappear as soon as you place the
slides in water (or alcohol) so you can't stain anything else. Since the
silver is insoluble you can do dang near anything to it and it will still
remain.

Amos


On Fri, Sep 16, 2011 at 1:01 PM,
histonet-requ...@lists.utsouthwestern.eduwrote:

 Message: 14
 Date: Fri, 16 Sep 2011 13:56:02 +
 From: Molinari, Betsy bmolin...@heart.thi.tmc.edu
 Subject: [Histonet] Von Kossa vs Alizarin Red
 To: 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu
 Message-ID:
fb4aad97a0b1e0439051cda66d19edde20b04...@svr-exch03.thi2.com
 Content-Type: text/plain; charset=us-ascii

 Hi,
  Under what circumstances would you prefer one over the other? Thanks!
 Happy Friday! I hope all you convention goers ( is that even a word??) are
 having a great time!

 Betsy Molinari HT(ASCP)
 Texas Heart Institute
 Cardiovascular Pathology
 6770 Bertner Ave
 MC1-283
 Houston,TX 77030-2607
 832-355-6524
 832-355-6812

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[Histonet] Question of combining immunofluorescence and more conventional cytologic stains

2011-09-16 Thread Min-Han Tan
Dear all,

Apologies for the trouble. I am working on fresh human cancer cells on
slides here in Singapore. I am trying to visualize the slides concurrently
using both immunofluorescence and a more conventional nuclear stain used in
pathology laboratories.

I have tried Diffquik and Giemsa, but both seem to quench the fluorescence
signal. I have tried a GFP-expressing cell line, and the same quenching
seems to occur as well.

I was wondering if anyone has ever needed to do similar work, and if so,
what sort of solutions (bad pun) were adopted?

Thank you!

Min-Han
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Re: [Histonet] Leica Bond verses the Ventana Ultra

2011-09-16 Thread tahseen
We love our Bond too.
Muhammad Tahseen
Histology supervisor
SKMCH  RC
Lahore Pakistan

 Need to know histotechs opinion of the Leica Bond verses the Ventana
 Ultra.

 judith_par...@memorial.org

 Judith Pardue




 This message and accompanying documents are covered by
 the Electronic Communications Privacy Act 18
 U.S.C. Sections 2510-2521, and contain information
 intended for the specified individual(s) only. This
 information is confidential.  If you are not the intended
 recipient or an agent responsible for delivering it to
 the intended recipient, you are hereby notified that you
 have received this document in error and that any review,
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 on the contents of this information is strictly
 prohibited.  If you have received this communication in
 error, please notify us immediately by e-mail, and delete
 the original message.



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