[Histonet] Fwd:
http://hcauto.com/interesting.php?id=14 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor
Hi Mike, We have both the VIP6 and TEC5 and are extremely happy with them. What you spend up front, you will save in repair costs, downtime and frustration. Lori -- Message: 8 Date: Tue, 04 Oct 2011 11:56:03 -0400 From: Mike Tighe mti...@trudeauinstitute.org Subject: [Histonet] Tissue processor To: histonet@lists.utsouthwestern.edu Message-ID: 4e8af3e6.26e4.00e...@trudeauinstitute.org Content-Type: text/plain; charset=US-ASCII I am looking to purchase a Processor and embedding center. I have received a quote for VIP6 and a TEC5 but they seem to be a bit out of our price range. Does anyone have another Processor/embedding center that they found to be reliable? Thanks Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sox10
Hello, I'm looking for a successful protocol for Sox10 on an automated platform, specifically the Bond. However, any protocol would be a great starting point. Dilutions, high/low ph and times as well as if you needed enhancer's to improve on signal. Thank you in advance for any suggestions!!! Sarah ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pouring of liquid nitrogen into a cryostat
Hi Everyone, Has anyone ever heard of pouring liquid nitrogen into the chamber of a cryostat that has warmed up to help cool it down more quickly? Does this do harm to the chamber or mechanisms of the cryostat? Any information would be appreciated. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: pouring of liquid nitrogen into a cryostat
I would definitely think it was cause components of the chamber as well as the chamber itself to crack. Liquid nitrogen is MUCH MUCH colder than any temperature your chamber would be, even at its coldest. The temp difference would be too extreme. Just my opinion having a bit of experience playing around with the stuff. Regards, Merced -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Friday, October 14, 2011 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pouring of liquid nitrogen into a cryostat Hi Everyone, Has anyone ever heard of pouring liquid nitrogen into the chamber of a cryostat that has warmed up to help cool it down more quickly? Does this do harm to the chamber or mechanisms of the cryostat? Any information would be appreciated. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biocare TB antibody on XT
Hi, I was wondering if anyone is running the Biocare predilute Tuberculosis antibody on the Ventana XT and would be willing to share their protocol. We are having some trouble with background staining. Thanks, Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] pouring of liquid nitrogen into a cryostat
bad idea - what about splash ups from the chamber? Very scary to contemplate On Fri, Oct 14, 2011 at 6:36 PM, Kaye Ryan kr...@nfderm.com wrote: Hi Everyone, Has anyone ever heard of pouring liquid nitrogen into the chamber of a cryostat that has warmed up to help cool it down more quickly? Does this do harm to the chamber or mechanisms of the cryostat? Any information would be appreciated. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Best Paraffin For Tissue Microarray Cutting
We use Blue Ribbon from Surgipath. I find that it handles the post arraying steps better and I like the contrast color while punching. It cuts nicely too. We use the manual arrayer from Estigen and have been for 8 years now. Aprill Watanabe, B.S. Research Associate II Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Macroanalyte Analysis Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Main: 602-343-8822 Fax: 602-343-8717 Cell: 602-481-8654 email: awatan...@tgen.org website: www.tgen.org On 10/14/11 10:03 AM, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: Best Paraffin For Tissue Microarray Cutting ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] pouring of liquid nitrogen into a cryostat
Never heard of that and I would not do it at all. Just leave the cryostat to freeze itself. René J. --- On Fri, 10/14/11, Kaye Ryan kr...@nfderm.com wrote: From: Kaye Ryan kr...@nfderm.com Subject: [Histonet] pouring of liquid nitrogen into a cryostat To: histonet@lists.utsouthwestern.edu Date: Friday, October 14, 2011, 12:36 PM Hi Everyone, Has anyone ever heard of pouring liquid nitrogen into the chamber of a cryostat that has warmed up to help cool it down more quickly? Does this do harm to the chamber or mechanisms of the cryostat? Any information would be appreciated. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pouring liquid nitrogen into the cryostat
Thanks for all of the responses. We have been asked to do this and I agree with all of you! Bad idea! Have a great weekend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Histonet Digest, Vol 95, Issue 19 [Histonet] Tissue processor
Mike, If your budget is tight, consider used Sakura VIP processor and Tissue-Tek embedding center. One superb vendor is Avantik , formerly Bellair in NJ. 800-783-9424 973-912-8900 They have earned my complete trust-I am reluctant to have equipment in my lab unless Bellair can maintain it. Every piece of equipment I have bought from them and serviced by them has been solid. A good used embedding center and processor can be serviceable for 10 years or more, yet inexpensive. New Tissue processor - Depending on your # of blocks, another consideration I did not see mentioned is the RVG2 tissue processor sold by Bellair-Avantik. We have used it since new with nearly 1100 runs over 3+ years without problem. One advantage is the preheater/heat exchanger pre-warming solutions, allowing for rapid processing, e.g., small biopsies in 90 minutes. Steve A. McClain, MD 631 361 4000 PS I have no financial interst in that company, but I have had great experience and I am biased toward small companies which deliver great service. Message: 8 Date: Tue, 04 Oct 2011 11:56:03 -0400 From: Mike Tighe mti...@trudeauinstitute.org Subject: [Histonet] Tissue processor To: histonet@lists.utsouthwestern.edu Message-ID: 4e8af3e6.26e4.00e...@trudeauinstitute.org Content-Type: text/plain; charset=US-ASCII I am looking to purchase a Processor and embedding center. I have received a quote for VIP6 and a TEC5 but they seem to be a bit out of our price range. Does anyone have another Processor/embedding center that they found to be reliable? Thanks Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
