[Histonet] Methyl green going away...

Hi my boss requires me to counterstain some DAB immunohistochemistry with methyl green. I use for that purpose the methyl green in aquos solution 0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate and the methyl green becomes extremely faint with the alcohols. My

[Histonet] CAP Question

I have a question regarding a new CAP checklist question dealing with ER/PR validation.  The new question is ANP.22976 ER/PgR Validation and states:   “If the laboratory performs immunohistochemistry for estrogen receptor (ER) and/or progesterone receptor (PgR) as a prognostic/predictive

[Histonet] A big thanks

I would like to say a big thank you to everyone who replied and sent me ideas to get the wrinkles out of the diaphysis of the mouse tibias I was cutting. Will be trying out some of the tips I received. Thanks again Nancy Lowen ___ Histonet mailing list

RE: [Histonet] CAP Question -ER/PR Validation

You should pose that question to CAP via acc...@cap.org they answer quickly and when they do you can print out their answer and keep for when your inspectors come. When I asked them that question, they said that it was usually at the discretion of the pathologist in charge. But instead of

RE: [Histonet] CAP Question

Shaundra, The CAP requirements are for those who are starting ER/PR for the first time, or changing antibodies to a new clone. If you did the validation years ago before those recommendations came out and have a history of running it successfully then you do not have to re-validate the

[Histonet] Re: Histonet Digest, Vol 101, Issue 21

Message: 1 Date: Mon, 16 Apr 2012 11:26:47 -0400 From: Alonso Martinez-Canabal alonso.martinezcana...@utoronto.ca Subject: [Histonet] Methyl green going away... To: histonet@lists.utsouthwestern.edu Message-ID: 000901cd1be5$5c48d470$14da7d50$@utoronto.ca Content-Type: text/plain;      

[Histonet] Tissue Processor

Hi everyone, I was wondering if anyone had any input on the Logos tissue processor by Milestone. Is anyone currently using it and what are the pros and cons, etc. . Any information would be greatly appreciated. Thanks, Vicki Gauch AMCH Albany, NY

[Histonet] Re: IHC in porcine tissues

Dear Colleagues  What  secondary antibody or polymer would be better to use for mouse primary antibody on FFPE  porcine tissues?Also, please share your experience regarding macrophages detection in porcine arteries. I found couple in Abcam and Serotec (only for frozen), but your opinions is

RE: [Histonet] Re: IHC in porcine tissues

Galina Try MAC387 that is a macrophage/monocyte marker that cross reacts in porcine. We use the one from abcam. We have found that some of the anti-mouse polymer reagents cross react to porcine so we use a rabbit anti-mouse from Dako E0464 as a secondary and then an envision rabbit or strept

RE: [Histonet] Methyl green going away...

You could try dehydrating in acetone (very quick). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead