[Histonet] DFA Method for the diagnosis of PCP on sputa.
Dear All, Good Morning, We are going to start DFA Method for the diagnosis of Pneumocystis carinii pneumonitis from Sputa or Tracheal Aspirates from Humans. Would anyone like to share a method/protocol for DFA? Thanks and good weekend to all Muhammad Tahseen MLT (Punjab medical Faculty) MT (Japan) Senior Supervisor Histopathology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] In FL, who can perform IHC/ISH?
I had an assignment in SW Florida not too long ago, doing mostly IHC, and at that time I was told that FL requires a tech doing IHC to be a Florida registered Technologist. (Florida has three levels of registration, technician, technologist, and supervisor.) *NOTA BENE*: This is NOT the same thing as an HTL (ASCP). To find the requirements for a Florida registered Technologist, go the the FL DOH website. It's almost time to renew mine. :) Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) : ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] DFA Method for the diagnosis of PCP on sputa.
Use Grocott's methenamine silver method. It has become the standard procedure for P.carinii René J. --- On Sat, 6/2/12, tahs...@brain.net.pk tahs...@brain.net.pk wrote: From: tahs...@brain.net.pk tahs...@brain.net.pk Subject: [Histonet] DFA Method for the diagnosis of PCP on sputa. To: histonet@lists.utsouthwestern.edu Date: Saturday, June 2, 2012, 3:30 AM Dear All, Good Morning, We are going to start DFA Method for the diagnosis of Pneumocystis carinii pneumonitis from Sputa or Tracheal Aspirates from Humans. Would anyone like to share a method/protocol for DFA? Thanks and good weekend to all Muhammad Tahseen MLT (Punjab medical Faculty) MT (Japan) Senior Supervisor Histopathology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Stains
Use modified Steiner with phosphotungstic acid instead of uranyl nitrate. René J. --- On Fri, 6/1/12, Dianne E. Holmes dhol...@umc.edu wrote: From: Dianne E. Holmes dhol...@umc.edu Subject: [Histonet] Stains To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Friday, June 1, 2012, 6:04 PM Does anyone know a retic stain that does NOT use uranyl nitrate? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] In FL, who can perform IHC/ISH?
This is correct. The only exclusion is a private office lab only needs to meet CLIA sub part M requirements. The science courses basically CLIA has been lenient on this but are now getting tuffer with the new healthcare regulations coming out. I don't want to make this a threat but everyone needs to be diligent About upping their game. It's Saturday and I really don't want to get blasted Read CLIA sub part M and go to DOH fl site like jay recommended Cheers Sent from my iPhone On Jun 2, 2012, at 11:07 AM, Jay Lundgren jaylundg...@gmail.com wrote: I had an assignment in SW Florida not too long ago, doing mostly IHC, and at that time I was told that FL requires a tech doing IHC to be a Florida registered Technologist. (Florida has three levels of registration, technician, technologist, and supervisor.) *NOTA BENE*: This is NOT the same thing as an HTL (ASCP). To find the requirements for a Florida registered Technologist, go the the FL DOH website. It's almost time to renew mine. :) Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) : ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] In FL, who can perform IHC/ISH?
All sorry about my double posting. My iPhone must have an echo. :( Enjoy life! Sent from my iPhone On Jun 2, 2012, at 1:11 PM, Kim Donadio one_angel_sec...@yahoo.com wrote: This is correct. The only exclusion is a private office lab only needs to meet CLIA sub part M requirements. The science courses basically CLIA has been lenient on this but are now getting tuffer with the new healthcare regulations coming out. I don't want to make this a threat but everyone needs to be diligent About upping their game. It's Saturday and I really don't want to get blasted Read CLIA sub part M and go to DOH fl site like jay recommended Cheers Sent from my iPhone On Jun 2, 2012, at 11:07 AM, Jay Lundgren jaylundg...@gmail.com wrote: I had an assignment in SW Florida not too long ago, doing mostly IHC, and at that time I was told that FL requires a tech doing IHC to be a Florida registered Technologist. (Florida has three levels of registration, technician, technologist, and supervisor.) *NOTA BENE*: This is NOT the same thing as an HTL (ASCP). To find the requirements for a Florida registered Technologist, go the the FL DOH website. It's almost time to renew mine. :) Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) : ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pinkus' acid orcein Giemsa stain
Luis Chiriboga (at NYU) asks for a method for Pinkus' acid orcein Giemsa stain. I haven't performed this stain or seen it done, but I copied the method out of Pinkus' book quite a few years ago. I don't know if anybody is still doing this stain. Orcein is a natural dye (from a species of lichen) which can be synthesized, though the synthetic dye is different from the natural one. Pinkus specified the synthetic and gave a source (the old Harleco). Bob Richmond Samurai Pathologist Knoxville TN Mehregan, Amir H. (Wayne State). Pinkus’ Guide to Dermatohistopathology. 4th ed. Appleton-Century-Crofts, 1986. Hermann Pinkus, 1905-1985 Acid orcein and Giemsa stain: from Pinkus H, Hunter R. Simplified acid orcein and Giemsa technique for routine staining of skin sections. Arch Dermatol 82:699, 1960 Krobock E, Rahbari H, Mehregan AH. Acid orcein and Giemsa stain. Modification of a valuable stain for dermatologic specimens. J Cutan Pathol 5:37, 1978. Fix in formalin or alcohol, without chromium or mercury. 1. Stain in ORCEIN for 30 minutes. Synthetic orcein stains elastic fibers specifically, with very little background staining. The background may be decolorized by short immersion in absolute alcohol or 0.1% acid alcohol. 2. Wash in running water 10 minutes. 3. Stain overnight in GIEMSA. Krobock et al. speeded this up by staining 1 hour in 1% Giemsa solution at 60° C. 4. Wipe slides. Remove excess blue by rinsing in 95% alcohol to which a small amount of eosin has been added if necessary. Continue until the collagen of the skin looks pink. Then dehydrate. ORCEIN: dissolve 200 mg of Harleco’s synthetic Orcein in 100 mL of 70% alcohol. Add 0.6 mL of concentrated HCl. The solution improves on standing and has a long shelf life. GIEMSA: one drop of stock in 20 mL of distilled water or pH 7.0 phosphate buffer. RESULTS: Collagen is rose-pink, while elastic is dark brown to black. Melanin is dark green to black. Bacteria and fungi are dark blue. Looking at the book, I would suppose that Mehregan had just about abandoned the use of this stain. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BLock alignment tool
Hi, There is one on every microtome made. The two little knobs align the block just perfectly to any angle previously cut. It is much better than just whacking into a block that was cut slightly differently than what a dumb instrument is telling you is aligned. Knowing how to use this alignment tool should be the first lesson. (Well ok maybe the second, the first being don't cut yourself!) This is like driving down the road with a clamp on your steering wheel complaining about the curve in the road causing you to hit a tree. Amos On Fri, Jun 1, 2012 at 10:32 AM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 2 Date: Thu, 31 May 2012 13:48:26 -0500 From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] BLock alignment tool To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 55c1fd599e1ffb479baa2e4b5ef348c3013c53bef...@mmc-mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii Awhile back we got an advertisement for a block alignment tool that would be helpful in recut cases. Can anyone share with me companies that have this device and approximate costs? James Vickroy BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] BLock alignment tool
I thought a alignment tool was one of those weird things histotechs used to make sure their toenails were even on all 5 toes ? Dang! Sent from my iPhone On Jun 2, 2012, at 2:31 PM, Amos Brooks amosbro...@gmail.com wrote: Hi, There is one on every microtome made. The two little knobs align the block just perfectly to any angle previously cut. It is much better than just whacking into a block that was cut slightly differently than what a dumb instrument is telling you is aligned. Knowing how to use this alignment tool should be the first lesson. (Well ok maybe the second, the first being don't cut yourself!) This is like driving down the road with a clamp on your steering wheel complaining about the curve in the road causing you to hit a tree. Amos On Fri, Jun 1, 2012 at 10:32 AM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 2 Date: Thu, 31 May 2012 13:48:26 -0500 From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] BLock alignment tool To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 55c1fd599e1ffb479baa2e4b5ef348c3013c53bef...@mmc-mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii Awhile back we got an advertisement for a block alignment tool that would be helpful in recut cases. Can anyone share with me companies that have this device and approximate costs? James Vickroy BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Retic Stains
The Gordon and Sweets, Gomori, Laidlaw, and Nasher and Shanklin methods for reticulin do NOT use Uranyl nitrate as a sensitizer. The Snook and Wilder methods do. Robert L. Lott, HTL(ASCP) From: Dianne E. Holmes dhol...@umc.edu Subject: [Histonet] Stains To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Friday, June 1, 2012, 6:04 PM Does anyone know a retic stain that does NOT use uranyl nitrate? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
