RE: [Histonet] Smooth muscle cell actin
Patti, The best antibody (much better than any rabbit, goat or even the same 1A4 clone conjugated with FITC) are: Mouse monoclonal Anti-alpha-smooth muscle actin Cy3 conjugated, Sigma cat.No.C6198. Works in dilution 1:500-1:2000 on formaldehyde fixed cell cultures, cryosections (both from fresh frozen and formaldehyde fixed samples, paraffin after formalin etc). I work with it last 8 years without any problem. They are relatively expensive, but last for long due to high working dilution. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patricia Bourassa Sent: Thursday, March 07, 2013 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Smooth muscle cell actin Howdy! Need to attempt immunostaining of smooth muscle cell actin in rat lung. Any recommendations for reliable SMC primary? Thanks in advance! Patti -- *Patti Bourassa Senior Scientist Karos Pharmaceuticals (203) 535-0540, ext 207* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: DAB source needed
I love Sigma DAB Tablets, they come in 1-5 and 15 MLs and you have to dissolve in water. The only problem is that they do not have any expression date. Soofia From: Blazek, Linda lbla...@digestivespecialists.com To: Sandra Cheasty cheas...@svm.vetmed.wisc.edu; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, March 6, 2013 9:48 AM Subject: [Histonet] RE: DAB source needed I have retired from shoveling but try Bio Care for your DAB. They have a very nice one. Also if you find someone to shovel send them my way! Although Wisconsin to Ohio might be tricky. But thanks for sending the snow our way. :) Linda Linda Blazek HT (ASCP) Manager/Supervisor Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty Sent: Wednesday, March 06, 2013 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB source needed Hello All, Can anyone recommend a DAB kit that is reliable and reasonable priced? Also, can someone shovel my driveway? Thanks! Sandy Sandra Cheasty Histology Necropsy Supervisor UW-Madison, Wisconsin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question on Estrogen antibodies for fish
I am asking this for a researcher. Please respond to her at klar_elizab...@columbusstate.edumailto:klar_elizab...@columbusstate.edu. Her name is Ely Klar. She is looking for any form of estrogen antibody that works on bass fish gonads. Vendor's responses welcome. Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problem with DAB staining
We would need to see the entire set of steps as there may be a variety of causes. Robert Schoonhoven, HT/HTL (ASCP) From: Nicole Cosenza ncose...@siumed.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 7, 2013 12:50 PM Subject: [Histonet] problem with DAB staining Hello all: I recently did IHC on paraffin embedded slides via the DAB method. Upon addition of the DAB, the entire section turned brown. I know this is not true staining, as I don't expect a high yield of my protein of interest. Any suggestions as to why this happened and what to do about it? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problem with DAB staining
Section should not dry in any stapes. Tahseen On 2013-03-07 22:50, Nicole Cosenza wrote: Hello all: I recently did IHC on paraffin embedded slides via the DAB method. Upon addition of the DAB, the entire section turned brown. I know this is not true staining, as I don't expect a high yield of my protein of interest. Any suggestions as to why this happened and what to do about it? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome upgrade, planning stages...
Add another Leica to your list René J, From: kgrob...@rci.rutgers.edu kgrob...@rci.rutgers.edu To: histonet histonet@lists.utsouthwestern.edu Sent: Thursday, March 7, 2013 2:32 PM Subject: [Histonet] Microtome upgrade, planning stages... We are starting to look into upgrading to higher-end, but used, microtomes. What we have is still working, but the writing is on the wall for them in terms of repair/parts. Which brands/models are considered higher-end in the used market these days? (Leica is already on my list, but which models?) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Kudos
Thanks to everyone for your suggestions and protocols for the IDH1 antibody. It helped immensely and now I don't have to beat my head against the wall anymore! Billie Zimmerman MT(ASCP)QIHC Laboratory Clinical Operations Mgr. BF 117 706-721-5617/3630 Fax: 706-434-4883 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SOP numbering for whole lab
Our histology SOPs start with H- (to distinguish them from other area SOPs) then each is assigned a unique chronological 4 digit ID number followed by the version number. For example: H-0001.00 where 0001 is the number for a specific SOP and .00 refers to the original version. The number for the first revision of this SOP would be H-0001.01. It's simple and every SOP and revision has a unique number. We keep master lists of the SOPs and the SOPs are under document control as well so each has its own history file. Betty Pollock Diagnostic Services Unit Manager Faculty of Veterinary Medicine University of Calgary Calgary, AB, Canada T3R 1J3 Tel: 403-220-2806 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: March 4, 2013 3:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SOP numbering for whole lab Help? What kind of formula do you use when numbering and coding your SOPs for the lab? Is there an easy way to keep them sorted and in line with all the regulatory bodies to help on inspection days? Any suggestions welcome--we're starting from scratch. Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Has anybody ever used...
Leica's CoolClamp, or Thermo's Cool Cut? These sound like a possible good solution for the inevitable days when the aging AC plant here can't keep up with the summer heat. Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
