[Histonet] RE: Negative Reagent Controls in Diagnostic IHC....
Okay so my question is.how did you address the non- use if you happen to be required? (Joint Commission) Beatrice L. Sullivan Corporate Histology Supervisor Virtua Voorhees -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Wednesday, October 09, 2013 4:59 PM To: Histonet Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?. I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Negative Reagent Controls in Diagnostic IHC....
Richard, I wish we could eliminate them but not only do our negatives displaying some non-specific staining, seems to be tissue dependent, but our in-house QA people say we need to continue using them per the data sheets accompanying many of our antibodies. We use Ventana UltraView DAB detection, a multimer kit. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Wednesday, October 09, 2013 3:59 PM To: Histonet Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?. I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] India Ink for inking surgical margin borders
I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. Does anyone know if I can used the artist india ink for Pathology use? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecre...@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] India Ink for inking surgical margin borders
The only warning I know of and I have used India Ink is to be sure it is the permanent India Ink not the washable. If you buy the non-permanent it will come off in processing. Pam Marcum - Original Message - From: Ed Crespo ecre...@cmblabs.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 10, 2013 11:53:17 AM Subject: [Histonet] India Ink for inking surgical margin borders I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. Does anyone know if I can used the artist india ink for Pathology use? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecre...@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Novec 7100 Engineerd Fluid
Hello to all in histoland. Is there anyone using the Novec 7100 fluid for freezing frozen blocks in a low temp freezing bath. We were getting it from fisher but they have discontinued it. I am trying to find another vendor. Any help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Novec 7100 Engineerd Fluid
Hi Allison, We use Novec 7000, but we also had to switch suppliers. Now it is purchased direct through the 3M company. You will find it on their website. Nancy Thomas Stowers Institute for Medical Research -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, October 10, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Novec 7100 Engineerd Fluid Hello to all in histoland. Is there anyone using the Novec 7100 fluid for freezing frozen blocks in a low temp freezing bath. We were getting it from fisher but they have discontinued it. I am trying to find another vendor. Any help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: India Ink for inking surgical margin borders
Ed Crespo, CT(ASCP) in Cypress CA asks: I normally purchase India Ink from one of our vendors, but know it's also sold at artist supply shops. Does anyone know if I can use the artist india ink for Pathology? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. I've used india ink from artist supply stores for marking surgical margins, for many years, and it's entirely satisfactory. A cheap source of colored particulate inks is tattoo inks - available in a huge range of colors - I have one pathologist client who's used them for years. The only downside is that you have to read some seriously yucky catalogs. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] India Ink for inking surgical margin borders
India Ink = India ink, no matter where you get it from. rené J. From: Ed Crespo ecre...@cmblabs.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 10, 2013 12:53 PM Subject: [Histonet] India Ink for inking surgical margin borders I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. Does anyone know if I can used the artist india ink for Pathology use? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecre...@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 119, Issue 13
Dear Histonetters, I am so happy to discover a new CD31 antibody on mouse tissues through Histonet. It really works well. Now I need p16 antibody that is good for FFPE mouse tissues. Appreciate your help as always! Regards, Mesru mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: India Ink for inking surgical margin borders
Tattoos Yucky? Guess I'm yucky. I got yucky way before tattoos became popular when the response was more akin to fringe element. Happy member of the Fringe. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, October 10, 2013 11:43 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: India Ink for inking surgical margin borders Ed Crespo, CT(ASCP) in Cypress CA asks: I normally purchase India Ink from one of our vendors, but know it's also sold at artist supply shops. Does anyone know if I can use the artist india ink for Pathology? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. I've used india ink from artist supply stores for marking surgical margins, for many years, and it's entirely satisfactory. A cheap source of colored particulate inks is tattoo inks - available in a huge range of colors - I have one pathologist client who's used them for years. The only downside is that you have to read some seriously yucky catalogs. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] External UV for a Leica 1850?
Hello Fellow Netters, Has anyone tried using some type of external UV source to decontaminate a Leica 1850 cryostat? I found out that it is not possible to retro fit the 1850 for UV. I would like to be able to avoid having to defrost, breakdown and bleach the cryostat everytime a suspected infectious tissue is cut in it. Suggestions kindly welcomed. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fluorochrome conjugated polymers for antibody detection
Hello; Can anyone tell me if there are anti-rabbit polymers (secondary antibody) that are conjugated to fluorchromes ? Or are the only polymers available conjugated to HRP? Susan Wert, PhD Cincinnati Children's Hospital Research Foundation Perinatal Institute/Pulmonary Biology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thermo Fisher Slide Mate printer
We are using them at Penn Medicine Dermatopathology. We interface with our homegrown LIS. We like the printers. We have had some ribbon issues causing the print to move and some numbers to be cut off and the ink on the fisher slide can be wiped off so we are looking at other slides to see if they are more resistant to chemicals and oils with that printer, but overall we have been happy with them. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Moe, Barbi A Sent: Thursday, October 10, 2013 3:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Fisher Slide Mate printer If anyone is currently utilizing this printer, could you please share pros and cons of your experience? Specifically interested if anyone has the unit interfaced with Power Path computer system. Thank you! Barb Moe Gundersen Health System 1910 South Ave La Crosse WI 54601 ba...@gundersenhealth.orgmailto:ba...@gundersenhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative Reagent Controls in Diagnostic IHC....
I would like to see the PP slide. I was unable to attend in Providence. Joelle Weaver MAOM, HTL (ASCP) QIHC From: richard.car...@hhchealth.org To: histonet@lists.utsouthwestern.edu Date: Wed, 9 Oct 2013 20:58:38 + Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?. I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thermo Fisher Slide Mate printer
We use the Slide Mate and we really like them. We did have one problem with the printer tape and then we got a different lot and things have been much better. The slides we use are the Thermo Colorfrost slides, in 6 colours and we have had no issues with the ink smearing or coming off. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Thursday, October 10, 2013 2:58 PM To: Moe, Barbi A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo Fisher Slide Mate printer We are using them at Penn Medicine Dermatopathology. We interface with our homegrown LIS. We like the printers. We have had some ribbon issues causing the print to move and some numbers to be cut off and the ink on the fisher slide can be wiped off so we are looking at other slides to see if they are more resistant to chemicals and oils with that printer, but overall we have been happy with them. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Moe, Barbi A Sent: Thursday, October 10, 2013 3:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Fisher Slide Mate printer If anyone is currently utilizing this printer, could you please share pros and cons of your experience? Specifically interested if anyone has the unit interfaced with Power Path computer system. Thank you! Barb Moe Gundersen Health System 1910 South Ave La Crosse WI 54601 ba...@gundersenhealth.orgmailto:ba...@gundersenhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] External UV for a Leica 1850?
If you are a CAP accredited lab, CAP says that the cryostat must be defrosted and disinfectant decontaminated at regular intervals with a TB disinfectant. - - - ANP.23410 Cryostat Decontamination Phase II There is a documented procedure for the routine decontamination of the cryostat at defined intervals, and decontamination records are evident. NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed surfaces with tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. This should be done at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. - - - - Even if you can use a UV light, ALL debris/contaminants must be removed from the cryostat chamber BEFORE using the UV light. The germicidal effect of radiation is only good on the areas that the UV light can hit directly. So any little corners, or areas under metal plates, or areas under the OCT/tissue shavings will not be directly illuminated by the UV light, and thus will not be disinfected. There are also different types of UV lamps. I have heard that low efficiency UV lamps need a long period of time of being turned on to disinfect, and that this long exposure in a small area of the chamber of the cryostat can produce a high level of ozone in the chamber, so there could be an ozone exposure level to the tech using the cryostat. So, UV light can be used in CONJUNCTION with wiping out, chemical disinfecting, and defrosting. But I don't believe it can be used IN PLACE of wiping out, chemical disinfecting, and defrosting. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Paula Sicurello Sent: Thursday, October 10, 2013 2:36 PM To: HistoNet Subject: [Histonet] External UV for a Leica 1850? Hello Fellow Netters, Has anyone tried using some type of external UV source to decontaminate a Leica 1850 cryostat? I found out that it is not possible to retro fit the 1850 for UV. I would like to be able to avoid having to defrost, breakdown and bleach the cryostat everytime a suspected infectious tissue is cut in it. Suggestions kindly welcomed. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AFB Controls
Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngfl...@yahoo.com Cheryl Kerry, HT(ASCP) 281.852.9457 Office 800.756.3309 Phone Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AFB Controls
Sigma provides AFB control slides if you can't get block. The item number is A2299 Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wine...@novusint.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Thursday, October 10, 2013 4:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Controls Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngfl...@yahoo.com Cheryl Kerry, HT(ASCP) 281.852.9457 Office 800.756.3309 Phone Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB Controls
I know source medical products has them cheaper than newcomer supply. E-mail and ask for Walter. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com On Thu, Oct 10, 2013 at 5:24 PM, Cheryl tkngfl...@yahoo.com wrote: Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngfl...@yahoo.com Cheryl Kerry, HT(ASCP) 281.852.9457 Office 800.756.3309 Phone Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB Controls
Make your own. Take some fresh lung (slightly edematous is better, if you can get it). Cut into 2x2 mm cubes (or largest 3x3 mm cubes). Contact Microbiology, and have them make a broth with a non-pathogenic AFB in a large tube (e.g., plastic centrifuge tube). Put lung cubes in broth. Incubate overnight (I found that room temp is usually OK). Next morning, add 10% NBF. Wait about an hour or so, then dispose of NBF, and add fresh 10% NBF. (The first NBF is diluted by the broth, and by allowing the NBF to sit in the broth for a while, it kills the bacteria. Adding the 2nd NBF allows the tissue to be fixed in 10% NBF, rather than diluted NBF.) Allow to fix most of the day, put tissue in cassettes, process as usual, embed, and you have lots of AFB controls for really cheap. Write up a cost containment (how much it cost you to make X number of blocks that you can get Y number of control slides from vs. the cost of buying the same number of Y slides from a vendor.) Management will love you for your cost containment. Works for gram +, gram -, and fungus (get the correct broth). Will work for spirochete too, but our micro lab has only a LARGE non-pathogenic spirochete, which is much larger than syphilis. So doing a Steiner stain would most likely yield a false-negative. (When the large spirochetes control is seen, the little syphilis would not be stained.) Please realize, these controls may not work for IHC. Better check on the genus. They work great for Kinyoun/Ziehl-Neelsen/Fites, Brown and Hopps/Brown and Brenn, GMS/PAS, Steiner/Warthin-Starry. Peggy Wenk, HTL(ASCP)SLS -Original Message- From: Cheryl Sent: Thursday, October 10, 2013 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Controls Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngfl...@yahoo.com Cheryl Kerry, HT(ASCP) 281.852.9457 Office 800.756.3309 Phone Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Negative Reagent Controls in Diagnostic IHC....
Hi Richard, I would appreciate a copy of the power point. thanks, Andrea On 2013-10-09, at 4:58 PM, Cartun, Richard richard.car...@hhchealth.org wrote: My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?. I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] External UV for a Leica 1850?
Paula, A UV insert in available from Hacker Instruments. (1-800-4-HACKER) It was not designed specifically for an 1850, but I don't see why it would not work out for you. Give Jim Mullen a call, he should be able to give you the device dimensions and power specifications. Have a great day. Dorothy Dorothy Traczyk BS, HT(ASCP) Histology Technician Monmouth Medical Center Long Branch, NJ 07740 In a message dated 10/10/2013 2:36:42 P.M. Eastern Daylight Time, pat...@gmail.com writes: Hello Fellow Netters, Has anyone tried using some type of external UV source to decontaminate a Leica 1850 cryostat? I found out that it is not possible to retro fit the 1850 for UV. I would like to be able to avoid having to defrost, breakdown and bleach the cryostat everytime a suspected infectious tissue is cut in it. Suggestions kindly welcomed. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Novec 7100 Engineerd Fluid
3M™ Novec™ Engineered Fluid HFE-7100 is made by 3M. They should be able to tell you how to get it. I haven't had my hands on it yet for frozen sections, but it's being very highly recommended. Bob Richmond Samurai Pathologist Maryville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: AFB Controls
With due deference to Peggy Wenk, I'm a little hesitant about AFB and other bacterial and fungal controls prepared by artificially inoculating normal lung tissue, particularly with AFB other than Mycobacterium tuberculosis. I don't understand why people don't use animal material here. Surely some enterprising vendor could find a researcher who's infecting guinea pigs with Myco. tuberculosis. The best AFB control material I've ever seen came from rhesus monkeys imported from India (a practice now prohibited) for research purposes. The veterinarians tuberculin tested the monkeys, put down the ones that tested positive, and autopsied them. Perfectly preserved tissue, loaded with red bugs, and HIPAA doesn't give a hoot about monkeys. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: India Ink for inking surgical margin borders
I use Artist China ( India ) Ink. The secret is to immerse the blocks in Bouin’s Fixative to coagulate the Ink. After that put the tissue blocks directly in the First Alcohol. Don’t go back to formalin. Cesar Romero Buenos Aires Argentina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: India Ink for inking surgical margin borders
Pam Marcum notes that The only warning I know of (and I have used India Ink) is to be sure it is the permanent India Ink not the washable. If you buy the non-permanent it will come off in processing. India ink is a suspension of carbon black (basically soot) in a suitable vehicle. It's quite permanent - there is no washable India ink. Ask at the art supply store if you're unsure of what you're buying. if you blot the specimen thoroughly dry before you ink it, you don't need fixatives for the ink like acetic acid, acetone, or Bouin fixative. I never use them. Ink won't stick to a cauterized surface (like a LEEP or a lumpectomy specimen) but the pathologist can see those cauterized margins under the microscope anyway. I didn't say tattoos were yucky - I said the catalogs were yucky. But bear with an old man who doesn't think they make girls any more like they did in 1955 (fortunately I've got one). As more and more restrictions are put on the tools grossing pathologists, PAs, and technologists can have, it becomes more important to know how to obtain tools and supplies in the real world. I can't replace my 25 year old Satterlee amputation saw, so I cut fractured femoral heads with a seven dollar hacksaw I bought at Home Depot. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
