[Histonet] 1. IHC wet workshop announcement (Workshop IHC)
Does anyone know of any similar IHC courses on the east coast? I'd love to attend this one, but won't be able to swing the travel costs. Thanks! Message: 1 Date: Tue, 14 Jan 2014 08:41:40 -0800 From: Workshop IHC workshop...@gmail.com Subject: [Histonet] IHC wet workshop anouncement To: histonet@lists.utsouthwestern.edu Message-ID: CAEa=20X1JbbBV-7hASHTQiKSdJwqv6G2j=Y+dpB9h00BMn=k...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Learn IHC staining, Hands - on IHC Lab courseCEU approved; February 27th 28th, 2014 San Francisco Bay area. For more info contact Maria workshop...@gmail.com -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ah...@georgetown.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Frozen sections (UNCLASSIFIED)
Classification: UNCLASSIFIED Caveats: NONE Hi LeAnn, Please note my response below. -Original Message- From: Bassett, Juan L CTR (US) Sent: Wednesday, January 15, 2014 7:39 AM To: 'Michael LaFriniere' Cc: 'stonet-boun...@lists.utsouthwestern.edu' Subject: RE: Frozen sections (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Michael, Based on my experience the pathologist or pathologists asst. are qualified to submit the specimen to be sectioned during a Frozen section procedure. And the pathologist asst. is allowed with the pathologist's approval/acknowledgement. Qualified HT and HTL's can gross non complicated specimens within normal tissue grossing procedures. There could be legal issues involved with a technician's unilateral decision during a frozen section procedure! To get more accurate feedback on this topic , I suggest you contact the ASCP with your consideration. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael LaFriniere Sent: Tuesday, January 14, 2014 1:46 PM To: Leann M. Murphy; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Frozen sections Hi Leann, Dependant on the case and how much tissue comes in from the OR, In my many years...I have on many occasions grossed and placed tissue for FS on chuck, and performed the frozen section, had it under the scope looking at it prior to the Pathologist coming in for DX.(why) because we have experience and it's a comfort level the pathologists under their guidance and supervision, Many of us have the experience to do this especially if you are grandfathered or have the required education under the CLIA regs to gross. I don't know of any regulation other than what I have mentioned above that would not allow the experienced HT to perform this operation. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafrini...@ccplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Monday, January 13, 2014 10:35 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen sections Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Frozen sections (UNCLASSIFIED)
Hi Juan, I am not sure of any ASCP guidelines nor jurisdiction over CLIA requirements. It is my understanding all US laboratories have to follow the CLIA set guidelines for performing all laboratory testing. Qualified HT or HTLs can gross complicated (high Complexity) testing under specific CLIA guidelines. CLIA guidelines have a grandfather clause for high complexity testing of experienced techs prior to (I think the exact date is) Sept 1996 that can demonstrate experience of the high complexity testing. Following Sept 1996 the educational guidelines set in for any high complexity laboratory testing requirements. Michael -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bassett, Juan L CTR (US) Sent: Wednesday, January 15, 2014 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Frozen sections (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Hi LeAnn, Please note my response below. -Original Message- From: Bassett, Juan L CTR (US) Sent: Wednesday, January 15, 2014 7:39 AM To: 'Michael LaFriniere' Cc: 'stonet-boun...@lists.utsouthwestern.edu' Subject: RE: Frozen sections (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Michael, Based on my experience the pathologist or pathologists asst. are qualified to submit the specimen to be sectioned during a Frozen section procedure. And the pathologist asst. is allowed with the pathologist's approval/acknowledgement. Qualified HT and HTL's can gross non complicated specimens within normal tissue grossing procedures. There could be legal issues involved with a technician's unilateral decision during a frozen section procedure! To get more accurate feedback on this topic , I suggest you contact the ASCP with your consideration. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael LaFriniere Sent: Tuesday, January 14, 2014 1:46 PM To: Leann M. Murphy; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Frozen sections Hi Leann, Dependant on the case and how much tissue comes in from the OR, In my many years...I have on many occasions grossed and placed tissue for FS on chuck, and performed the frozen section, had it under the scope looking at it prior to the Pathologist coming in for DX.(why) because we have experience and it's a comfort level the pathologists under their guidance and supervision, Many of us have the experience to do this especially if you are grandfathered or have the required education under the CLIA regs to gross. I don't know of any regulation other than what I have mentioned above that would not allow the experienced HT to perform this operation. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafrini...@ccplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Monday, January 13, 2014 10:35 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen sections Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Warthin Starry Help
Thank you so much. These tips seem very helpful. Could you give me your protocol? Times in temps in the impregnation step. And your measurements for each reagent in the developing solution. I use acidulated water and I usually get the pH at about 3.8. I am staining for h.Pylori. Like I said, when I first started making my own reagents, I actually made up enough of each for a week and the stain was working beautifully. But after a couple months, it has just mysteriously stopped working. I just don't get it. I can use a kit and the sections will stain wonderfully but just cant get my own reagents to work and I am trying to save money since I am such a small lab. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: Tony Reilly [mailto:tony.rei...@health.qld.gov.au] Sent: Tuesday, January 14, 2014 7:38 PM To: Campbell, Tasha M.; histonet@lists.utsouthwestern.edu Subject: RE: Warthin Starry Help Hi Tasha I have been performing the Warthin-Starry successfully for nearly 40 years. Without seeing your method these are a few tips that should be followed. 1. Make sure all solutions are made fresh each time using an acetate buffer or acidulated water as diluent. I prefer an acetate buffer. 2. Because of the difficulty in controlling development with this method, more than one section and control should be stained, with shorterand longer development times. 3. Sections must not be allowed to dry before flooding with the developer solution, as this will cause precipitation of silver, making results difficult to interpret. 4. Do not wash slides between pouring off the silver solution and adding the developer. 5. Ensure that the developer is made up just before use and is up to temperature. Add silver nitrate to dissolved gelatin, stirring continuously. Ensure this is done slowly to prevent the formation of precipitate. Just before time of use, add hydroquinone. 6. Allow sections on the first pair of slides to develop until the background appears yellow to light golden brown. This will take approximately 5-7 minutes. Allow the second pair of slides to develop until the background appears brown to dark brown. This may take up to an additional 3-5 minutes. Regular microscopic examination is required to check for reaction endpoints. Flood slides with acetate buffer or acidulated water once endpoints are reached to prevent further silver development. 7. Fixatives containing chrome and mercuric salts should not be used as they can interfere with the reaction, giving false negative results. 8. Discard developer once it becomes too dark. If sections are underdeveloped they can be placed into a second batch of fresh developer solution for another 3-5 minutes. 9. The method will demonstrate a number of argyrophilic organisms. If a particular infectious agent is suspected, use a control section containing that organism. If the cause of infection is in doubt, use a control containing Spirochaetes. If this is successfully impregnated it canvirtually be assumed that all other argyrophilic organisms would be demonstrated. 10. Melanin and other argentaffin or argyrophilic substances may also be demonstrated. 11. Some methods wash in hot tap water following development, I prefer to rinse in hot acetate buffer or acidulated water first to avoid any background precipitation of silver. I hope this is of some use. Regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld 4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony.rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tasha M. Campbell Sent: Wednesday, 15 January 2014 12:55 AM To: Tony Reilly; histonet@lists.utsouthwestern.edu Subject: [Histonet] Warthin Starry Help I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori
[Histonet] FISH technique and NBF as fixative
I need to do FISH with the universal bacterial probe EUB338. I am not too familiar with FISH. Can we fix the sample with NBF or is 4% paraformaldehyde better? The samples are acellular dermal matrix. A quick lit search shows people using 4% paraformaldehyde, but the histologist that will be embedding the tissue said that NBF is gentler on tissue than 4% PFA... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HER2/NEU
I just wanted to see what others in the histology world might be experiencing. We have seen a marked increase in the percentage of HER2 cases resulting as Indeterminate, when we compare our last year patient predictive marker results to this year's results. The clone we are using is CB11 from Cell Marque. We did a HER2 study last year where we sent out our cases for a second opinion, and the study showed others interpreted our Indeterminates as that also. Has anyone else also noted this increase in their annual comparison, and what clone are you using? It is so nice to be able to reach out to our peers with these types of questions. Thank you for responding back! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknut...@primecare.orgmailto:dknut...@primecare.org This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HER2/NEU
That is an interesting observation, as we have seen something of the same thing and we use the Dako Herceptest.I would be interested in hearing others responses as well. Thank you for asking the question. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Wednesday, January 15, 2014 11:46 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HER2/NEU I just wanted to see what others in the histology world might be experiencing. We have seen a marked increase in the percentage of HER2 cases resulting as Indeterminate, when we compare our last year patient predictive marker results to this year's results. The clone we are using is CB11 from Cell Marque. We did a HER2 study last year where we sent out our cases for a second opinion, and the study showed others interpreted our Indeterminates as that also. Has anyone else also noted this increase in their annual comparison, and what clone are you using? It is so nice to be able to reach out to our peers with these types of questions. Thank you for responding back! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknut...@primecare.orgmailto:dknut...@primecare.org This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: HER2/NEU
Consider the ASCO-CAP guideline and scoring changes for Her2/ FISH, not sure of the timeframe for your trend in increased positives, but my pathologist has indicated that I should expect more positive and equivocal cases, so perhaps related to categorizing of DX, rather performance or clone related.? see if that correlates Joelle Weaver MAOM, HTL (ASCP) QIHC From: mw...@wakehealth.edu To: dknut...@primecare.org; histonet@lists.utsouthwestern.edu Date: Wed, 15 Jan 2014 17:04:10 + CC: Subject: [Histonet] RE: HER2/NEU That is an interesting observation, as we have seen something of the same thing and we use the Dako Herceptest.I would be interested in hearing others responses as well. Thank you for asking the question. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Wednesday, January 15, 2014 11:46 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HER2/NEU I just wanted to see what others in the histology world might be experiencing. We have seen a marked increase in the percentage of HER2 cases resulting as Indeterminate, when we compare our last year patient predictive marker results to this year's results. The clone we are using is CB11 from Cell Marque. We did a HER2 study last year where we sent out our cases for a second opinion, and the study showed others interpreted our Indeterminates as that also. Has anyone else also noted this increase in their annual comparison, and what clone are you using? It is so nice to be able to reach out to our peers with these types of questions. Thank you for responding back! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknut...@primecare.orgmailto:dknut...@primecare.org This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recuts/deepers
This is my first time working in a GLP/GMP lab, Ive only been here for 6 months. We cut a variety of tissue. At trimming is where they decide what tissue to go in what cassette and so one cassete could have 4-6 different types of tissue. And on the other hand we do osteoinduction studies, and when we get recuts for the OI it means that our first section given to the pathologist did not pass the requirement. The pathologist sometimes list what they are looking for and I have noticed that the problem is the tissue was not embedded at the same plane in the block. I do admit I have cut shallow before because I did not want to go too deep to get the other section and lose the tissue before it (example: adrenals). My plan after I get opinions/advice on histonet is to go talk to the pathologists and study directors. Thank you for your help!!! Trini ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat Decontamination
I am looking for a procedure and product to use for decontamination after a potential TB case, help please! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2014 CPT Changes
Hello, I know this subject had been looked at many times. I just want to see if I am correct before I start doing anything different. 1. 88342 , not much change . We can still just bill once per site, per case. This is per single antibody per Specimen. If I have a case with two parts and each part has 3 immuno's ordered I will bill them all 88342, Even if I do it on multiple blocks of the same specimen, I will still charge once. If one of these antibodies is a cocktail, I will bill 88342 for the first and 88343 for subsequent parts of that cocktail, and I can do this per specimen. So the only change I see here is the breakdown of the cocktail charges into 88343. 2. Medicare codes, GO461 is ordered for the first antibody , per specimen site per case, all additional antibodies, including cocktails , if any, are billed GO462. As before only 1 antibody can be billed by site/source. Now just to work out how the billing will flip over once the charges go across. Is anybody doing it differently? Susie Hargrove HT (ASCP) Histology Technical Specialist United Regional Health Care Wichita Falls, Texas 76301 Ph 940-764-3881 Fax-940-764-3129 CONFIDENTIALITY NOTICE: This e-mail, and any attachment, may contain identifiable health information that is subject to protection under state and federal law. This information is intended only for the person or entity to which it is addressed. If you are not the intended recipient, be aware that any review, re-transmission, copying, dissemination or other use of this information by persons or entities other than the intended recipient is prohibited and may be punishable by law. If you received this in error, please return it to the sender by electronic mail (reply) and delete the material from any computer or server on which it resides due to your receipt. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat
Need to decon my cryostat Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Do I have to destabilize MMA?
I am new to MMA plastic bone technique. Some one gave me his protocol, in which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. But others told me I don't need to do the destabilization step. Could any expert in this area to tell me if this step is necessary? And why have to do? Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
