[Histonet] Alport's testing
Does anyone test for Alport's (alpha 1, 3 5 chains of collagen IV) on paraffin sections? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.nationwidechildrens.org/ One person with passion is better than forty people merely interested. ~ E.M. Forster ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recommendations cryostat
Hi All, We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. Regards Muhammad Tahseen SKMCHRC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene substitute for processing GI biopsies
Theoretically YES! René J. On Sunday, April 20, 2014 7:36 PM, Joe W. Walker, Jr. joewal...@rrmc.org wrote: Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub? We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewal...@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition® and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. René J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt loreli...@mac.com wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining. The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Paraffin
I am still trying to figure out what I need to do for validation for CAP. Do I need to revalidate IHC and/or special stains if I change the paraffin on the processor? The paraffin I have in mind is supposed to work faster than the one we currently use for infiltration, so we may need to adjust times. Thanks. From: Boyd, Debbie M [dkb...@chs.net] Sent: Wednesday, April 16, 2014 10:17 AM To: Cameron, Elizabeth; histonet@lists.utsouthwestern.edu Subject: RE: Paraffin We use Richard Allen Type 6 paraffin for both infiltration and embedding (16 years) with no adverse effects. To validate you can get the pathologist to give you samples for the same specimens you are running. IE; gallbladder 1 for patient dx and 1 for testing, uterus, appendix, any large specimen that you can spare an extra slice. Run the patient dx specimens one night, change only the paraffin in your processor and embedding center run the test samples during the day while the processor isn't in use for patient specimens. Cut and stain per usual as time permits. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cameron, Elizabeth Sent: Wednesday, April 16, 2014 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Hello histonetters, I recently made the move back to a hospital lab after being in research, and we are currently using two different parrafins for infiltration and embedding. I would like to change the type of paraffin that we are using (I have one in mind), but I was wondering if there were any advantages to using different paraffins for the different steps and what type of validation would need to be done to make this change. We only have one processor, so a side by side comparison would be very difficult. Any thoughts on the matter would be greatly appreciated. Thank you, Liz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] COM 30450
Hello Everyone, Does COM 30450 apply to Diff Quik kits and reagents used in special stains (other stain kits)? Or does the lot to lot testing only apply to things such as IHC? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Recommendations cryostat
Best recommendation I can provide is the Leica 1950 was the hands down choice of the employees performing frozen sections at multiple hospital sites in my system. From my perspective, the instruments are very reliable, easy to use, easy to maintain and pathologists approve of the the slide quality. William DeSalvo, BS HTL(ASCP) From: hi...@skm.org.pk To: histonet@lists.utsouthwestern.edu Date: Mon, 21 Apr 2014 14:01:36 + CC: tahseen0...@gmail.com Subject: [Histonet] Recommendations cryostat Hi All, We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. Regards Muhammad Tahseen SKMCHRC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recommendations cryostat
Leica! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor Sent: Monday, April 21, 2014 7:02 AM To: histonet@lists.utsouthwestern.edu Cc: tahseen0...@gmail.com Subject: [Histonet] Recommendations cryostat Hi All, We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. Regards Muhammad Tahseen SKMCHRC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] anti mouse iNOS
Hi Everyone! Anyone have a good antibody for an anti mouse iNOS antibody that they especially like? Thanks! Anna Anna C. Hughes anna.c.hug...@gsk.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Recommendations cryostat
Definitely Leica Sent from the iPhone of Kim Tournear On Apr 21, 2014, at 7:35 AM, Bea DeBrosse-Serra bdebrosse-se...@isisph.com wrote: Leica! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor Sent: Monday, April 21, 2014 7:02 AM To: histonet@lists.utsouthwestern.edu Cc: tahseen0...@gmail.com Subject: [Histonet] Recommendations cryostat Hi All, We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. Regards Muhammad Tahseen SKMCHRC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Xylene substitute for processing GI biopsies
Joe, Yes. We are about to go xylene-free (though we are still planning on using if for cleaning, because the feedback we got was that we still needed to clean the processors with xylene or we would run into problems, and my supervisor heard from people that they had to go back to xylene flushing if they initially stopped). We are going with an odorless substitute because the orange-smelling ones bother some people in the lab. We did a full validation (with blinded evaluations) that included special stains and IHC with all retrieval methods, and Breast Imaging. CAP validation requirements are getting stricter, and new IHC validation guidelenes just came out from CAP, so I would recommend validating. FYI-routinely our pathologists selected the slides with substitute processing over xylene processed (though it was close). Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CRYOSTAT RECOMMENDATIONSRE: Histonet Digest, Vol 125, Issue 24
Good ;Morning, We have the Leica 1950 in our Laboratory and are extremely pleased with the insturment. Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital - South Zone West - Alberta Health Services Lethbridge, Alberta, Canada karen@albertahealthservices.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: April 21, 2014 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 125, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Xylene substitute for processing GI biopsies (Joe W. Walker, Jr.) 2. Alport's testing (Houston, Ronald) 3. Re: Xylene substitute for processing GI biopsies (Rene J Buesa) 4. Recommendations cryostat (Pathology-Histology Sr. Supervisor) 5. RE: Paraffin (Cameron, Elizabeth) 6. COM 30450 (Piche, Jessica) 7. RE: Recommendations cryostat (WILLIAM DESALVO) 8. RE: Recommendations cryostat (Bea DeBrosse-Serra) 9. anti mouse iNOS (Anna Hughes) 10. Re: RE: Recommendations cryostat (Kim Tournear) -- Message: 1 Date: Sun, 20 Apr 2014 23:36:05 + From: Joe W. Walker, Jr. joewal...@rrmc.org Subject: RE: [Histonet] Xylene substitute for processing GI biopsies To: Rene J Buesa rjbu...@yahoo.com, Lori Gemeinhardt loreli...@mac.com,histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 3c2378778400ad448ada6fd6bdb7182a0...@rrmbx03.rrmc.local Content-Type: text/plain; charset=iso-8859-1 Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub? We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewal...@rrmc.orgwww.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt loreli...@mac.com wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining. The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from
[Histonet] Semen Analysis Training
Does anyone know of a facility in the North Jersey or New York City area that has a semen analysis training program. We are looking for motility and morphology. We are familiar with the Cleveland Clinic program but it is a distance as well as expensive. Any input or suggestions would be helpful. Chris D The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
