RE: [Histonet] Re: Negative Controls
So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Negative Controls
As of now, my lab took that as you just needed one negative slide per run. And that negative slide is getting the negative mouse or rabbit serum. Is this correct? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PT
I need to enroll in a Proficiency testing program just for the technical part. I am looking for the cheapest program that CLIA would recognize. I am only CLIA certified. Thanks. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Negative Controls
Tasha, We use the negative elements within our patient sample as our negative tissue control. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Negative Controls
So do we. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, April 30, 2014 8:35 AM To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls Tasha, We use the negative elements within our patient sample as our negative tissue control. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Negative Controls
So do we Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, April 30, 2014 8:35 AM To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls Tasha, We use the negative elements within our patient sample as our negative tissue control. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including
[Histonet] Special stain control validation- CAP compliance
Hello, We validate our control blocks prior to patient use. We label the slide with the stain, date, and results. We keep the slides in a file for reference. Our recent CAP inspection cited us on this because it was not documented on paper. I am considering challenging this, as I feel what we're doing meets the intent of the checklist question. What are your opinions? Thanks, Stacy **NEW** 07/29/2013 ANP.21460 Special Stain Controls Phase I Validated tissue controls are required for each special stain. NOTE: Positive tissue controls assess the performance of the special stain. Special stains are performed on sections of control tissue known to contain components specific to each special stain. Validation of tissue used as a positive control must be performed and documented before being used with clinical specimens. Evidence of Compliance: ✓ Written results of special stain control tissue validation [Cooley-Dickinson.org] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Question about high complexity testing for histotechnologists
NO, because the license in histotechnology INCLUDES high complexity testing. On the other hand there are some tests like FISH with the VYSIS system that requires a special training with its own certification. René J. On Tuesday, April 29, 2014 10:15 PM, Delia, Catherine deli...@uhnj.org wrote: Does anyone know if there is a regulatory agency that requires high complexity testing certification for histotechnologists. Catherine Susan Delia, BS. HT. ASCP Chief Technologist, Anatomic Pathology University Hospital 150 Bergen Street E-151 Newark, New Jersey 07103 Phone: 973-972-5717 Cell: 908-391-1060 Fax: 973-972-5724 deli...@uhnj.orgmailto:deli...@uhnj.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP question ANP.22978 - Her2 assay validation
https://www.biomax.us/tissue-arrays/Breast/ theres the link, they have various kinds, so just choose the one that comes with the IHC results Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: Martha Ward-Pathology [mailto:mw...@wakehealth.edu] Sent: Wednesday, April 30, 2014 7:11 AM To: Vanessa Perez Subject: RE: CAP question ANP.22978 - Her2 assay validation Thanks. I like the idea of the microarray slide as well. Where did you purchase your slide? martha -Original Message- From: Vanessa Perez [mailto:vpe...@pathreflab.com] Sent: Tuesday, April 29, 2014 5:18 PM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: CAP question ANP.22978 - Her2 assay validation From what I have read and understand you should be able to do a write up the retroactive review based on the PT results. What we did here was bought a microarray slide that came with the HER2/ER/PR results, ran them on our machine, and compared our results to the ones that came with the slide. Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, April 29, 2014 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question ANP.22978 - Her2 assay validation Hello all, I have been reading through the most recent revisions and want to see how others are handling this question. The explanation states that it is for new and existing assays and that if your validation does not meet current standards that you must supplement and bring it into compliance. Furthermore if you do not have any documentation from the initial validation the assay must be fully revalidated and documented. Our lab has been performing the Herceptest from Dako (FDA approved) since before 2008 and participating in the HER2 proficiency testing since it was first offered. We have our statistical results comparing our IHC patient results to FISH Her2 results since 2008 and we have always done well on our CAP proficiency testing (95%-100%).We do inter-pathologist result comparisons, using know CAP slides and have 95% to 100% agreements. What I do not have however is the original results of the slides that were stained to set up the original assay. Under these circumstances will we need to completely revalidate the assay, using the mandated 20+/20- cases, or can we simply do a retroactive formal review and write up of our past performances on our proficiency testing challenges? Thanks in advance for your help with this! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thank You!
All- A very sincere thank you to everyone who has responded so graciously to my request for information for Seventh Wave Pathology and for additional suggestions for CRO Histology/Pathology services. I have forwarded the information on. Thank you again for the great feedback! Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] animal human biopsies
Some threw the thought of processing biopsies from veterinary clinics as a way of increasing outpatient volumes. I am concerned there may be a regulation against this. Does anybody know of any? Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin fixation problems
Recently we started having problems with our 10% nb formalin not completely fixing our animal tissues. We haven't done any thing different in making up the solutions, but we're finding incomplete fixation in the center of the tissues. We are letting it stay in formalin the same amount of time (24-48 hrs) as we've always done. The pH has not changed. Does any one have any suggestions as to where we can look to trouble shoot correcting this problem? Our recipe method is: 4g Sodium Phosphate, monobasic monohydrate(Fisher Chemical co.) 6.5g Sodium Phosphate, dibasic anhydrous(EMD Chemical co.) 37% Formaldehyde(EMD Chem.co.) 10 parts formaldehyde to 90 parts Dh20. Any helpful thoughts would be appreciated. Thank you, Fran Pearsall HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Is there a way to post pictures of slides on histonet? Or to make them available for people to look at?
Hi everyone, What is a good way to post pictures of slides for you guys to look at? I had a weird black precipitate in one of my IHCs and I'd like for you guys to look at them and see if you recognize what this artifact is? Thanks Patrick. PS: I tried uploading them as attachments but the size was too large even though I tried to crop things to keep the size to a minimum. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Is there a way to post pictures of slides on histonet? Or to make them available for people to look at?
HI Patrick, Marvin Hanna posted these instructions in the below message on histonet yesterday. This is a good way to post images you wish to discuss on histonet. He has made available a way to do this for the histonet community, as well as has the archives of histonet on the www.histosearch.com website. You cannot post images on histonet. Shirley Powell Hi Histonetters, Tim Morken asked me about posting images for discussion on Histonet a couple of weeks ago and we noticed our images website is no longer running. As we move into digital pathology, having the ability to post images to discuss on Histonet is important. So, we set up an easy way to post images for Histonet on Histosearch at http://histosearch.com/imageupload/. You need to fill in your name, a title for your images, select images to upload from your computer or smartphone and click submit. There is no need to register and it takes about 2 minutes. We intentionally did not include comments on the website so the discussions will take place on Histonet. We have also set up mobile pages for searching Histosearch and the archives at http://m.histosearch.com and http://m.histosearch.com/histonet/ for histologists on the go. You may also want to try out the new image searching capabilities on Histosearch. After searching with keyword(s) such as ki67 or alcian blue, there is a link at the top of the results page for images. Clicking this will provide images related to your keyword(s) from the over 12 million histopathology related pages searched in Histosearch. We believe we have the largest list of histology educational resources on the internet at Histosearch and they are searchable for text and images. Let us know if we've missed any histology resources. Best Regards, Marvin Hanna -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Wednesday, April 30, 2014 1:43 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Is there a way to post pictures of slides on histonet? Or to make them available for people to look at? Hi everyone, What is a good way to post pictures of slides for you guys to look at? I had a weird black precipitate in one of my IHCs and I'd like for you guys to look at them and see if you recognize what this artifact is? Thanks Patrick. PS: I tried uploading them as attachments but the size was too large even though I tried to crop things to keep the size to a minimum. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Is there a way to post pictures of slides on histonet? Or to make them available for people to look at?
Hi Patrick, You can upload images for Histonet at http://histosearch.com/imageupload/. The Histonet listserver does not allow attachments to messages to prevent the spread of malware. Let me know if you have any problems. Best Regards, Marvin Hanna On 04/30/2014 01:43 PM, Lewis, Patrick wrote: Hi everyone, What is a good way to post pictures of slides for you guys to look at? I had a weird black precipitate in one of my IHCs and I'd like for you guys to look at them and see if you recognize what this artifact is? Thanks Patrick. PS: I tried uploading them as attachments but the size was too large even though I tried to crop things to keep the size to a minimum. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] I just posted 2 photos to histosearch
Thanks everyone, I just posted 2 images to histo search. IS there a way to post up to 5 pics at once? It looked like it suggested that you could post up to 5 pics as a group but I didn't see HOW to do it. Anyway, I have one post of outside of the tissue area, and one post of the tissue area. What is this weird black precipitate? I was using Red AEC as a substrate. Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Awards Spotlight - The Newcomer Helping Hand Award
Award Spotlight - The Newcomer Helping Hand Award This award is sponsored by Newcomer Supply Inc. and consist of a $1500.00 grant. This is a new award for the NSH it has only been given out once so far, last year was the first year it was given and Jean Mitchell NSH's Region IV director was its first recipient. Jean knows first-hand what it's like to help others internationally she was able to travel to Botswana, Africa in August 2013 as part of an ASCP Laboratory Mission to assess and teach good histotechnology practices in Botswana. This grant is intended to: Support national and or international histology education outreach efforts. Increase the quality and expertise of histology educational opportunities for student groups with limited or deteriorated access to educational facilities and educators. Affect efforts to recruit future generations of histotechnologists and promote the study of histology. Provide needed educational supplies or equipment. This grant can be used to support any educational opportunity such as hosting high school students in your laboratory or at a STEM event. Nominate yourself if you are planning such an event or a colleague if you know that they have been involved in educational opportunities in the past and are planning to do so in the future at the following link http://nsh.org/scholarships-awards/advocacy/service%20awards?nid=2930 This is just one way that this award can be used. Helping others both nationally and internationally learn about histology can be a really fun experience. We host students yearly in our lab and it is an exhausting but very rewarding experience. Getting our young students interested in science is in my opinion is a really worthy cause especially if I can do that by teaching them about histology. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.commailto:l...@premierlab.com www.premierlab.comhttp://www.premierlab.com/ March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] I just posted 2 photos to histosearch
Hi Patrick, To post more than one image, there is a link under choose file where you uploaded the first picture for add another image. When you click add another image, it opens up another choose file box for a second image to upload. Or you can just post them one at a time if you like. I've seen that black precipitate on slides in an IHC lab I was at a couple of years ago. If I remember correctly, we thought it was a formalin-heme pigment which can be caused when formalin is acidic (not buffered). Marvin On 04/30/2014 02:05 PM, Lewis, Patrick wrote: Thanks everyone, I just posted 2 images to histo search. IS there a way to post up to 5 pics at once? It looked like it suggested that you could post up to 5 pics as a group but I didn't see HOW to do it. Anyway, I have one post of outside of the tissue area, and one post of the tissue area. What is this weird black precipitate? I was using Red AEC as a substrate. Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] gmed/ggastro interfacing with pathology for seamless transition?
Hi all, Our practice is moving forward to bringing in-house pathology since we already have in-house histology. Any gastro practices that use gmed/ggastro and have a seamless pathology module that can be added for a smooth transition? This would entail using ggastro/gmed and interfacing with the pathology module. We currently use WINSURGE but cannot interface with ggmed/gastro easily. Any thoughts? Thanks, Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
