[Histonet] Bubbles in mounting media
Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bubble problems!
Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bubbles in mounting media
Hi Melissa Try to fill the tube of mounting media some hours before use. I use to fill it in the end of the day and in the next day the micro bubbles will be completely disappeared. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Melissa Ng Sent: Thursday, August 07, 2014 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubbles in mounting media Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: p16
Do you know what the dilution is from Biocare, and how many tests per vial? From: Weems, Joyce K. [joyce.we...@emoryhealthcare.org] Sent: Wednesday, August 06, 2014 5:02 PM To: 'Ronda Mire'; Burnett, Brandy Cc: histonet@lists.utsouthwestern.edu; sarah.dys...@stdavids.com Subject: RE: [Histonet] RE: p16 They bought the company! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ronda Mire Sent: Wednesday, August 06, 2014 3:37 PM To: Burnett, Brandy Cc: histonet@lists.utsouthwestern.edu; sarah.dys...@stdavids.com Subject: Re: [Histonet] RE: p16 Before Ventana had p16, it was only available from MTM labs, if I recall correctly the name. I am not sure if Ventana is the exclusive vendor now or not. Good luck. On Aug 6, 2014, at 3:13 PM, Burnett, Brandy bburn...@capecodhealth.org wrote: I am looking for one as well, I wasn't sure if they owned the patent. Let me know if you find one. Thanks! Brandy Burnett, HTL (ASCP) Cape Cod Hospital From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of sarah.dys...@stdavids.com [sarah.dys...@stdavids.com] Sent: Wednesday, August 06, 2014 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist. Thanks Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Bubble problems!
Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium should do it. Then, leave the bottle of mounting medium for about a day or two to allow any air that may have gotten mixed into to dissipate. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Melissa Ng melissa...@spdscientific.com Sent: Thursday, August 07, 2014 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubble problems! Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Bubble problems!
Melissa we are having the same problem. We use a different mounting medium. I will try Barbara's advice and try adding a bit of xylene to the media. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, August 07, 2014 8:12 AM To: Melissa Ng; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bubble problems! Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium should do it. Then, leave the bottle of mounting medium for about a day or two to allow any air that may have gotten mixed into to dissipate. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Melissa Ng melissa...@spdscientific.com Sent: Thursday, August 07, 2014 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubble problems! Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pay Wage
What is a good pay wage for a 25yr experience in Histology. Any good information out there ? Indiana based. S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbald...@mhhcc.orgmailto:sbald...@mhhcc.org:) Christ's healing mission of compassion empowers us to be for others through Quality and Excellence Vision Statement: We are committed to being the preferred health and wellness provider; transforming lives through faith based, compassionate care. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Negative slides:
Is it necessary to run a negative slide per IHC case per CLIA when using polymer based detection? Does anyone have references? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] questions
I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: questions
When I worked in clinical labs the order was first the priority cases (biopsies, bone marrows, etc.) then numerically. I remember once two physicians brought small biopsies and were in a hurry for the results, both wanted to be first out and I can still hear myself explaining to them that there could only be ONE first! Andi From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Webb, Dorothy L [dorothy.l.w...@healthpartners.com] Sent: Thursday, August 07, 2014 9:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: questions
... and I can still hear myself explaining to them that there could only be ONE first! Yes, DAILY!!! Every time someone wants to change things around so they get theirs first, this is our only defense as to why everyone can't get theirs first. 8 years of college doesn't seem to help here... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, August 07, 2014 10:08 AM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: questions When I worked in clinical labs the order was first the priority cases (biopsies, bone marrows, etc.) then numerically. I remember once two physicians brought small biopsies and were in a hurry for the results, both wanted to be first out and I can still hear myself explaining to them that there could only be ONE first! Andi From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Webb, Dorothy L [dorothy.l.w...@healthpartners.com] Sent: Thursday, August 07, 2014 9:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: questions
Our cases are cut by priority. And we don't process breasts. (children's hospital) Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, August 07, 2014 11:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p16
We use p16 on the BondMax at a 1:4 dilution. There are 11.5 ml in the container when received. Comes from Dako. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator Dubuque, IA * Message: 12 Date: Thu, 7 Aug 2014 13:06:37 + From: Burnett, Brandy bburn...@capecodhealth.orgmailto:bburn...@capecodhealth.org Subject: RE: [Histonet] RE: p16 To: Weems, Joyce K. joyce.we...@emoryhealthcare.orgmailto:joyce.we...@emoryhealthcare.org, 'Ronda Mire' rm...@cvpath.orgmailto:rm...@cvpath.org Cc: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu, sarah.dys...@stdavids.commailto:sarah.dys...@stdavids.com sarah.dys...@stdavids.commailto:sarah.dys...@stdavids.com Message-ID: c7ceb8be7df69446817c237018344d0004a...@fhexmb1.cchdomain1.capecodhealth.orgmailto:c7ceb8be7df69446817c237018344d0004a...@fhexmb1.cchdomain1.capecodhealth.org Content-Type: text/plain; charset=us-ascii Do you know what the dilution is from Biocare, and how many tests per vial? NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] questions
1. Our early (small biopsies)processor finishes at 4am. Anything with special instructions (Cyto blocks, liver bxs, pros bxs, Bone Marrow cases) are done first. Then just by numerical as they come out of the rack. Late processor finishes at 0630 and the first done are Breast bxs, sent node cases or other with special instructions. 2. We find that Small bxs and core bxs do well with less than 24 hours. Not sure if that is what you are looking for:) We have a primary embedder each day. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator * Message: 17 Date: Thu, 7 Aug 2014 11:54:00 -0500 From: Webb, Dorothy L dorothy.l.w...@healthpartners.commailto:dorothy.l.w...@healthpartners.com Subject: [Histonet] questions To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Message-ID: 65365f35c0f2ef4d846ec3ca73e49c4302f6d319b...@hpemx3.healthpartners.intmailto:65365f35c0f2ef4d846ec3ca73e49c4302f6d319b...@hpemx3.healthpartners.int Content-Type: text/plain; charset=us-ascii I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16
Are you sure Dako has p16? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, August 07, 2014 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 We use p16 on the BondMax at a 1:4 dilution. There are 11.5 ml in the container when received. Comes from Dako. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator Dubuque, IA * Message: 12 Date: Thu, 7 Aug 2014 13:06:37 + From: Burnett, Brandy bburn...@capecodhealth.orgmailto:bburn...@capecodhealth.org Subject: RE: [Histonet] RE: p16 To: Weems, Joyce K. joyce.we...@emoryhealthcare.orgmailto:joyce.we...@emoryhealthcare.org , 'Ronda Mire' rm...@cvpath.orgmailto:rm...@cvpath.org Cc: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern. edu histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern. edu, sarah.dys...@stdavids.commailto:sarah.dys...@stdavids.com sarah.dys...@stdavids.commailto:sarah.dys...@stdavids.com Message-ID: C7CEB8BE7DF69446817C237018344D0004A719@FHEXMB1.cchdomain1.capecodhealth .orgmailto:c7ceb8be7df69446817c237018344d0004a...@fhexmb1.cchdomain1.ca pecodhealth.org Content-Type: text/plain; charset=us-ascii Do you know what the dilution is from Biocare, and how many tests per vial? NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P16
Actually comes from Roche..sorry about that. Nancy Schmitt MLT, HT(ASCP) Histology Coordinator NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC QC forms:
Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: questions
Sounds like we are all working at the same place! LOL I hear exactly the same questions.is it something they teach to all pathology residents Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, August 07, 2014 1:18 PM To: 'Grantham, Andrea L - (algranth)'; Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: questions ... and I can still hear myself explaining to them that there could only be ONE first! Yes, DAILY!!! Every time someone wants to change things around so they get theirs first, this is our only defense as to why everyone can't get theirs first. 8 years of college doesn't seem to help here... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, August 07, 2014 10:08 AM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: questions When I worked in clinical labs the order was first the priority cases (biopsies, bone marrows, etc.) then numerically. I remember once two physicians brought small biopsies and were in a hurry for the results, both wanted to be first out and I can still hear myself explaining to them that there could only be ONE first! Andi From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Webb, Dorothy L [dorothy.l.w...@healthpartners.com] Sent: Thursday, August 07, 2014 9:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] questions
Priority driven block order, by specimen type/protocol, defined in SOP ( TAT for 'stat, ASAP routine) Fixation is monitored for all tissues, just easier to do all the same, made entry field in LIS, and tracking log for manual back up for computer down times. Keep to CAP guidelines for breast, 6-72, disclaimers when needed for alternate fixation, decal, or missing times such as for referrals where it is not sent or recorded. I am the only, technical person, but this is what I do for SOP as of right now. Congrats on up coming retirement! Joelle Weaver MAOM, HTL (ASCP) QIHC From: dorothy.l.w...@healthpartners.com To: histonet@lists.utsouthwestern.edu Date: Thu, 7 Aug 2014 11:54:00 -0500 Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC QC forms:
We only check and document control slide quality and need for repeat due to control failure. The pathologist reviewing the slides will send a QC form to us if there is a problem with case slides. Plus thye can enter a repeat for a poor stain as a repeat and we can track the number of repeats in the system. It is actually one of our QA monitors and review of repeats is documented in our quarterly QA review meeting. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Thursday, August 07, 2014 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QC forms: Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] On the lighter side...
How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Industry Standard
Friends - One of our metrics we monitor in our lab is a receive to sign out time metric. We measure the time the specimen comes to the lab to the time the case is signed out by a pathologist. We normally run on average 31-32 hours per case for our inpatients (about 1200 cases per month) but I wanted to ask this group if you measure a similar metric. If you do, was is your turn around time? Any articles on the industry standard for this? Thank you so very much for any/all help you can provide. Best, Matthew J. Lauterbach, MBA, MLS (ASCP)CM Laboratory Services Manager Phlebotomy/Campus Services/Histology/POC Avera McKennan Region Phone: (605) 322-7126 Cell: (605) 496-1272 Fax: (605) 322-7083 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On the lighter side...
36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Sullivan, Beatrice bsulli...@virtua.org To: 'Douglas Porter' doug.por...@caplab.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
39 years for me Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net Date: Thu, 7 Aug 2014 11:59:44 -0700 From: cont...@excaliburpathology.com To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Sullivan, Beatrice bsulli...@virtua.org To: 'Douglas Porter' doug.por...@caplab.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
46 - but I was 2 when I took the sat for the exam Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
29. I feel so young... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, August 07, 2014 1:51 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: questions
We embed and cut and by priority for: 1) bone marrows, 2) biopsies, 3) breast cases 4) surgicals, 5) placentas, 6) autopsies. RUSH cases for kidney, liver and heart take priority over all! Occasionally we will have requests for a specific case to be cut earlier than the usual priority list and will do that as needed. The cases are cut in numerical order for bone marrows and biopsies to make it easier to stain, organize and deliver them. (We don't have to look for them for an hour if they are in order and anything missing is immediately apparent.) Since we have four to five people cutting in the morning for surgicals we cut in numeric order by case and also alpha order again to help with staining, check out and delivery. Depending on the person sectioning faster or slower we may have cases out of numeric order at times on the stainer and at check out. We adhere strictly to the breast times for 6 to 72 hours in formalin as minimum and maximum limits. We would be prefer all breast have overnight fixation and that is not possible at times. We use the rule anything not in a cassette by 3PM cannot be processed overnight for breast. Then we know we have 6 hours based on time from gross to completion of formalin on the processors. The trick was to make them understand it had to be grossed and in a cassette by cut off not just soaking in formalin. We have residents. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, August 07, 2014 11:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
41 years. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Sullivan, Beatrice bsulli...@virtua.org To: 'Douglas Porter' doug.por...@caplab.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On the lighter side...
I just made 50. Started early. Pam - Original Message - From: Hazel V Horn hor...@archildrens.org To: Paula Pierce cont...@excaliburpathology.com, Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 2:09:40 PM Subject: RE: [Histonet] On the lighter side... 41 years. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Sullivan, Beatrice bsulli...@virtua.org To: 'Douglas Porter' doug.por...@caplab.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] questions
So here at TJUH, we are color coded and all blocks other than routine and decals are cut in this manner. One tech is assigned rapids (green), another tech is assigned bone marrow and cell blocks (yellow and blue) and one tech is assiged out satalite hospital (pink cassettes) All are completed by 10AM. The fourth tech will start on biopsies (peach). This is all done while embedding is still taking place. Routine and decals are placed in order qc'd an distributed among all tech except the rapid tech. They are all stained accordingly. We do adhere the the proper fixation times for IHC. Autopy cassettes are cut by a tech in the afternoon depending on who is available. We do not have any lab aides so a tech is assigned to instrumentation, and one tech is assiged up front, that is staining, sorting and releasing of cases. This tech is assisted by another tech when they have completed microtomy. The tech that is in the front will cut biopsies, especially the ones for H-Pylori and than go to the front for the remainder of the shift. I know it sounds confusing but it actually works. Sue Paturzo TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
41 years also!! Sharon Beckham Head, Histotechnology Stowers Institute for Medical Research Kansas City, MO -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, August 07, 2014 2:10 PM To: 'Paula Pierce'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 41 years. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Sullivan, Beatrice bsulli...@virtua.org To: 'Douglas Porter' doug.por...@caplab.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] formalin pigment and 10% NBF question
Hi everyone, As I read and was told, formalin pigment is seen as a dark precipitate under light microscope. I also read that you can see this via polarizing microscope as it is birifringent. I'm wondering: does it first need to be seen under the light microscope, and birifringence is just a confirmation? Or, can you see its birifringence without needing to see the precipitates under the light microscope? With regards to 10% NBF, do you guys pH this fixative if it's commercially prepared? Is this always reliably neutral? Thank you for your time! Cecilia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
13 years Michaela LeFaivre BS, HTL (ASCP) CM Molecular Technician IV Molecular Pathology Rm 4344 Purple Zone 919-684-4303 919-684-5095 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Shirley A. Powell [powell...@mercer.edu] Sent: Thursday, August 07, 2014 3:30 PM To: Paula Pierce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 52 years, if we are all posting. Lol -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Sullivan, Beatrice bsulli...@virtua.org To: 'Douglas Porter' doug.por...@caplab.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: On the lighter side
34 years. Egads, I'm OLD! When did that happen? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 12. On the lighter side... (Douglas Porter) Message: 12 Date: Thu, 7 Aug 2014 14:38:32 -0400 From: Douglas Porter doug.por...@caplab.org Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You?? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org -- Message: 13 Date: Thu, 7 Aug 2014 18:51:28 + From: Sullivan, Beatrice bsulli...@virtua.org Subject: RE: [Histonet] On the lighter side... To: 'Douglas Porter' doug.por...@caplab.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 6932520047f7ee46b512e9801344f1608002a...@exchangemb-1.virtua.org Content-Type: text/plain; charset=us-ascii 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: On the lighter side
Well I'm just a baby.in pathology 6 years...only! Sent from my iPhone On Aug 7, 2014, at 3:47 PM, Terri Braud tbr...@holyredeemer.com wrote: 34 years. Egads, I'm OLD! When did that happen? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 12. On the lighter side... (Douglas Porter) Message: 12 Date: Thu, 7 Aug 2014 14:38:32 -0400 From: Douglas Porter doug.por...@caplab.org Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You?? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org -- Message: 13 Date: Thu, 7 Aug 2014 18:51:28 + From: Sullivan, Beatrice bsulli...@virtua.org Subject: RE: [Histonet] On the lighter side... To: 'Douglas Porter' doug.por...@caplab.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 6932520047f7ee46b512e9801344f1608002a...@exchangemb-1.virtua.org Content-Type: text/plain; charset=us-ascii 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] please remove me from the list
This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
36 years here -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Sullivan, Beatrice bsulli...@virtua.org To: 'Douglas Porter' doug.por...@caplab.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
30years tomorrow. Lee Ann Baldridge IUSM -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sources of LVN (low viscosity nitrocellulose)
Historically, we used LVN (low viscosity nitrocellulose, also marketed as Celloidin and various other trade names) to embed specimens such as whole decalcified salmon heads for thick sectioning (30 to 100µm) using sliding microtomes such as the AO 860. I used to be able to purchase LVN powder wetted with alcohol from the Hercules Company. Because their LVN was not highly purified, it was much less expensive than Celloidin. It worked perfectly for our technique. Unfortunately, Hercules is no longer in business. Does anyone know of a commercial source for LVN? Willy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
Well...I appears most of us are right properly fixed. Good to see some newbies chiming in. This also shows that we need to get/keep more of the young ones interested in the field. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: Baldridge, Lee Ann [mailto:lhad...@iupui.edu] Sent: Thursday, August 07, 2014 3:56 PM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 30years tomorrow. Lee Ann Baldridge IUSM -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4744 / Virus Database: 3986/7994 - Release Date: 08/06/14 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
I have been recruiting exclusively in histology for 10 years. ; ) Thanks-Pam Right Place, Right Time, Right Opportunity. Pam Barker RELIA Solutions. rel...@earthlink.net (407) 353-5070 cell/text Sent from my Galaxy S®III div Original message /divdivFrom: Douglas Porter doug.por...@caplab.org /divdivDate:08/07/2014 4:10 PM (GMT-05:00) /divdivTo: 'Baldridge, Lee Ann' lhad...@iupui.edu, histonet@lists.utsouthwestern.edu /divdivSubject: RE: [Histonet] On the lighter side... /divdiv /divWell...I appears most of us are right properly fixed. Good to see some newbies chiming in. This also shows that we need to get/keep more of the young ones interested in the field. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: Baldridge, Lee Ann [mailto:lhad...@iupui.edu] Sent: Thursday, August 07, 2014 3:56 PM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 30years tomorrow. Lee Ann Baldridge IUSM -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4744 / Virus Database: 3986/7994 - Release Date: 08/06/14 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sources of LVN (low viscosity nitrocellulose)
Technically LVN and celloidin are not quite the same. LVN (also known as gun cotton) is useful for large specimens -we used to use this for aborted fetal heads and large bone specimens. It penetrates much more rapidly that celloidin and is much easier and safer to handle. Tissues prepared especially in celloidin are magnificent due to the very low rate of shrinkage. The cost of safe transport of these is prohibitive. I am retired now but in the 1990s celloidin from Fisher was $1,200 a pound plus transport costs. Barry . On Thu, Aug 7, 2014 at 3:01 PM, Willy Bemis we...@cornell.edu wrote: Historically, we used LVN (low viscosity nitrocellulose, also marketed as Celloidin and various other trade names) to embed specimens such as whole decalcified salmon heads for thick sectioning (30 to 100µm) using sliding microtomes such as the AO 860. I used to be able to purchase LVN powder wetted with alcohol from the Hercules Company. Because their LVN was not highly purified, it was much less expensive than Celloidin. It worked perfectly for our technique. Unfortunately, Hercules is no longer in business. Does anyone know of a commercial source for LVN? Willy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
35 years here. Histology has been very, very good to me! :-) Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, August 07, 2014 3:09 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 46 - but I was 2 when I took the sat for the exam Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
28 years here -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeff L Sent: Thursday, August 07, 2014 3:54 PM To: 'Paula Pierce'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 36 years here -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Sullivan, Beatrice bsulli...@virtua.org To: 'Douglas Porter' doug.por...@caplab.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
34 years for me, but it seems like just yesterday it was 33.999 years. Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Water/Bubbles
Hello all, Recently we have been experiencing a problem when running HE slides on our Gemini stainer (Thermo Shandon Varistain). At the end of the run, small water droplets or possibly air bubbles are seen on the slides after taking the slides out of xylene. If they are coverslipped, a white haze will be seen around the specimen after a few minutes. Any suggestions of what causes this and how to correct it? Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water/Bubbles
Hi Julie, We have the Gemini stainer and have noticed that water gets trapped in the top of the slide rack. Make sure the level of your dehydrating alcohols after the eosin are high enough to cover the top of the rack, and then make sure your xylene level is high enough to get rid of all the alcohol. Also, avoid shaking the excess xylene off of the rack when you take it off the stainer before coverslipping. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Julie Bowman Sent: Thursday, August 07, 2014 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water/Bubbles Hello all, Recently we have been experiencing a problem when running HE slides on our Gemini stainer (Thermo Shandon Varistain). At the end of the run, small water droplets or possibly air bubbles are seen on the slides after taking the slides out of xylene. If they are coverslipped, a white haze will be seen around the specimen after a few minutes. Any suggestions of what causes this and how to correct it? Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
Celebrated 1yr HTL in July Terra Wineman, HTL (ASCP)CM Research Biologist, Nutritional Physiology 636-926-7476 phone terra.wine...@novusint.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Thursday, August 07, 2014 3:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 34 years for me, but it seems like just yesterday it was 33.999 years. Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: on the light er side...
16 years!!! Kathy Machado, HTL Danville Regional Medical Center Danville, VA kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net 434-799-3867 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
15 Years and still going strong :) Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vriv...@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 129, Issue 10
44 years here On 8/7/14 1:26 PM, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: questions (Marcum, Pamela A) 2. Re: questions (Sue) 3. RE: On the lighter side... (Beckham, Sharon) 4. Re: On the lighter side... (Pam Marcum) 5. RE: On the lighter side... (Shirley A. Powell) 6. formalin pigment and 10% NBF question (Cecilia A.) 7. RE: On the lighter side... (Michaela Lefaivre) 8. please remove me from the list (Huimin Wang) 9. RE: On the lighter side (Terri Braud) 10. Re: RE: On the lighter side (Melissa Burns) 11. RE: On the lighter side... (Howery, Jeff L) 12. RE: On the lighter side... (Baldridge, Lee Ann) 13. Sources of LVN (low viscosity nitrocellulose) (Willy Bemis) 14. RE: On the lighter side... (Douglas Porter) 15. RE: On the lighter side... (Pam Barker) 16. Re: Sources of LVN (low viscosity nitrocellulose) (Barry Rittman) 17. RE: On the lighter side... (Mark Turner) 18. Water/Bubbles (Julie Bowman) -- Message: 1 Date: Thu, 7 Aug 2014 19:09:40 + From: Marcum, Pamela A pamar...@uams.edu Subject: [Histonet] RE: questions To: Webb, Dorothy L dorothy.l.w...@healthpartners.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 41d3a1af6fef0643bdc89e0516a6ea32012404d...@mail2node2.ad.uams.edu Content-Type: text/plain; charset=us-ascii We embed and cut and by priority for: 1) bone marrows, 2) biopsies, 3) breast cases 4) surgicals, 5) placentas, 6) autopsies. RUSH cases for kidney, liver and heart take priority over all! Occasionally we will have requests for a specific case to be cut earlier than the usual priority list and will do that as needed. The cases are cut in numerical order for bone marrows and biopsies to make it easier to stain, organize and deliver them. (We don't have to look for them for an hour if they are in order and anything missing is immediately apparent.) Since we have four to five people cutting in the morning for surgicals we cut in numeric order by case and also alpha order again to help with staining, check out and delivery. Depending on the person sectioning faster or slower we may have cases out of numeric order at times on the stainer and at check out. We adhere strictly to the breast times for 6 to 72 hours in formalin as minimum and maximum limits. We would be prefer all breast have overnight fixation and that is not possible at times. We use the rule anything not in a cassette by 3PM cannot be processed overnight for breast. Then we know we have 6 hours based on time from gross to completion of formalin on the processors. The trick was to make them understand it had to be grossed and in a cassette by cut off not just soaking in formalin. We have residents. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, August 07, 2014 11:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If
[Histonet] RE: re: on the light er side...
36 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kathy.mach...@lpnt.net Sent: Thursday, August 07, 2014 4:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: on the light er side... 16 years!!! Kathy Machado, HTL Danville Regional Medical Center Danville, VA kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net 434-799-3867 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On the lighter side...
31 years Jennifer MacDonald From: Douglas Porter doug.por...@caplab.org To: histonet@lists.utsouthwestern.edu Date: 08/07/2014 11:36 AM Subject:[Histonet] On the lighter side... Sent by:histonet-boun...@lists.utsouthwestern.edu How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On the lighter side...
Registered 4 years Doing histology 30 years. On Thu, Aug 7, 2014 at 5:19 PM, Jennifer MacDonald jmacdon...@mtsac.edu wrote: 31 years Jennifer MacDonald From: Douglas Porter doug.por...@caplab.org To: histonet@lists.utsouthwestern.edu Date: 08/07/2014 11:36 AM Subject:[Histonet] On the lighter side... Sent by:histonet-boun...@lists.utsouthwestern.edu How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
28 years Rick Carter -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 7, 2014 2:20 PM To: Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 31 years Jennifer MacDonald From: Douglas Porter doug.por...@caplab.org To: histonet@lists.utsouthwestern.edu Date: 08/07/2014 11:36 AM Subject:[Histonet] On the lighter side... Sent by:histonet-boun...@lists.utsouthwestern.edu How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 129, Issue 11
10 years certified, 12 total! :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
15 years and over 10 certified HTL. Claire Ingles From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Richard A. Carter [rcar...@cancer-test.com] Sent: Thursday, August 07, 2014 4:36 PM To: 'Jennifer MacDonald'; Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 28 years Rick Carter -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 7, 2014 2:20 PM To: Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 31 years Jennifer MacDonald From: Douglas Porter doug.por...@caplab.org To: histonet@lists.utsouthwestern.edu Date: 08/07/2014 11:36 AM Subject:[Histonet] On the lighter side... Sent by:histonet-boun...@lists.utsouthwestern.edu How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On the lighter side...
49 years and 45 years certified as HT and 22 as HTL Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com Tele: (831) 375-3577 X117 On Aug 7, 2014, at 3:39 PM, Ingles Claire cing...@uwhealth.org wrote: 15 years and over 10 certified HTL. Claire Ingles From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Richard A. Carter [rcar...@cancer-test.com] Sent: Thursday, August 07, 2014 4:36 PM To: 'Jennifer MacDonald'; Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 28 years Rick Carter -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 7, 2014 2:20 PM To: Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 31 years Jennifer MacDonald From: Douglas Porter doug.por...@caplab.org To: histonet@lists.utsouthwestern.edu Date: 08/07/2014 11:36 AM Subject:[Histonet] On the lighter side... Sent by:histonet-boun...@lists.utsouthwestern.edu How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Bubble problems!
Dear Hazel and Barbara, Thank you for your feedback. Wouldn't adding too much xylene result in drying effects post-coverslipping? Best thanks and regards, Melissa -Original Message- From: Horn, Hazel V [mailto:hor...@archildrens.org] Sent: Thursday, 7 August, 2014 10:10 PM To: 'Barbara Tibbs'; Melissa Ng; Histonet@lists.utsouthwestern.edu Subject: RE: Bubble problems! Melissa we are having the same problem. We use a different mounting medium. I will try Barbara's advice and try adding a bit of xylene to the media. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, August 07, 2014 8:12 AM To: Melissa Ng; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bubble problems! Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium should do it. Then, leave the bottle of mounting medium for about a day or two to allow any air that may have gotten mixed into to dissipate. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Melissa Ng melissa...@spdscientific.com Sent: Thursday, August 07, 2014 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubble problems! Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eons in Histology
I've been registered since 1969. This thread is interesting and I'd like to see what those of us Who Have Been in this Game so Long have as registry numbers. Mine is 04958 (I think...). How many of you can beat that? But who's counting? I retired 2.5 years ago but I'll always be a histotech! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Correction
I must be getting old - my ASCP number is 04258, not 04958 (or whatever number I first wrote down)... I hate it when that happens! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
