[Histonet] Pipette calibration with Artel PCS?
Hi. I'm not sure if Histonet is the best place to ask, but I was wondering if anyone out there is using - or have heard about - the Artel PCS system for pipette calibration as an alternative to the traditional gravimetric method? My work place is in the process of setting up its own pipette calibration instead of sending them away, and since we are out of good spaces to place a balance (climate controlled+vibration free+ergonomically OK) we are looking into options such as the PCS. The cost of aquiring the system would equal the cost of getting a balance with a table, and the PCS is said to be more accurate at the small volumes we use. Reagents are costly though... Any experiences? Nina Pries Dep of Clinical Pathology, Lund, Sweden ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: fish eggs: processing and fixation
Hi Christy, Do you have the facility for resin sectioning? This is the way to go for fish eggs. It's a long time since this type of processing was carried out in our labs but if you are able to process in this way I'm sure I would be able to dig out a protocol from our Thesis library. I also suspect 10%NBF will have to be replaced with another fixative. Regards Debbie Technical Specialist Fish Health and Welfare Laboratories Institute of Aquaculture University of Stirling Stirling, FK9 4LA Tel: +44(1)1786 466592/466590 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thompson, Christy Sent: 23 October 2014 19:39 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish eggs: processing and fixation Hi all, I'm currently working on some unfertilized fish eggs. They were fixed in 10% NBF and processed in a vacuum processor with paraffin. The eggs are extremely hard to section and most of the yolk goes AWOL on the way to the water bath. I did do a search here for some help and a few things were posted but one post in particular found some answers but they were not listed in his reply and I think the post was pretty old (but no date on it). Anyone out there have any success with sectioning fish eggs? Thanks in advance! Christy Thompson A/Histology Lab Leader - Aquatic Animal Health Pacific Biological Station Fisheries and Oceans Canada Nanaimo, BC 250-756-7060 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling has been ranked in the top 12 of UK universities for graduate employment*. 94% of our 2012 graduates were in work and/or further study within six months of graduation. *The Telegraph The University of Stirling is a charity registered in Scotland, number SC 011159. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: working on Saturday
We are a 242 bed hospital with a very active maternity unit and a huge women's health service. We get approx 10,000 cases/year. We find no advantage to working on Saturdays with the exception of a STAT cytology for an inhouse patient. Also, with the new recommended fixation times for breasts, we no longer even have one person for that little bit of Saturday coverage. There was not one peep from any physicians. Even when I used to manage a hospital lab twice the size, we did not cover Saturdays. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 1. Working on Saturday (Smallwood, Lorraine) - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: working on Saturday
We do have someone come in on Saturday for half a day. We have an average of 35 bone marrows a day and getting them cut and ready on Saturday is what we have to do to make Monday work. This is a teaching hospital and the residents love to submit everything but the kitchen sink on Fridays and Saturday mornings. We also have GI and Gyn clinics on Friday and the biopsy load goes up along with routine surgicals so Mondays can be very heavy. The person working the half day Saturday can pick day during the week to leave early. We have the same person on call for the week to cover RUSH transplant biopsies. (They rarely have to come in however; we still have to cover if needed.) The on call person answers any problems with processors or other issues. When we are full staff the cover is every fifth week, so it is not too bad. I cover for any open spaces or health issues. They prefer the supervisor not take call to avoid overtime. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Friday, October 24, 2014 8:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: working on Saturday We are a 242 bed hospital with a very active maternity unit and a huge women's health service. We get approx 10,000 cases/year. We find no advantage to working on Saturdays with the exception of a STAT cytology for an inhouse patient. Also, with the new recommended fixation times for breasts, we no longer even have one person for that little bit of Saturday coverage. There was not one peep from any physicians. Even when I used to manage a hospital lab twice the size, we did not cover Saturdays. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 1. Working on Saturday (Smallwood, Lorraine) - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 131, Issue 26
*Regarding processors* *I also need to help on determine which processor for our core. Our majority tissue is bone. * *Right now, we like VIP serials because of vacuum/pressure plus agitation. Please help me* *on this VIP processor, I never used this kind one. We are very small volume* *core, also do frozen and plastic undecal bone. We have a Shandon Excelsior now and feel the * *vacuum volume for this 10 years old processor is not enough for bone. * *Any input for this will be greatly appreciated. * *Dorothy* *MGH endocrine histocore* 9. RE: Processors (Joelle Weaver) 11. Re: Processors (Jay Lundgren) Message: 9 Date: Thu, 23 Oct 2014 19:05:23 + From: Joelle Weaver joellewea...@hotmail.com Subject: RE: [Histonet] Processors To: Jamal j.rowa...@alborglaboratories.com, 'GMail' nguy0...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: snt149-w792d852db98a977b194067d8...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Agree Joelle Weaver MAOM, HTL (ASCP) QIHC From: j.rowa...@alborglaboratories.com To: nguy0...@gmail.com; histonet@lists.utsouthwestern.edu Date: Thu, 23 Oct 2014 11:25:23 +0300 Subject: RE: [Histonet] Processors CC: hi colleague if your daily processed specimens less than hundred, go ahead for VIP5. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of GMail Sent: Thursday, October 23, 2014 12:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processors Please help me decide on a processor, I am currently inquiring about refurbished processors for a small derm path lab with very low volumes. I have quotes for VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k and a Leica TP1020 type 4 for $17. Which one would you recommend? What's durable and won't break down often? Should I go for the vip2000 compared to vip5 to save money? Sincerely? Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paraffin and Saturday work
We use Leica infiltration paraffin for the processors and their Blue Ribbon for embedding We work Saturdays to lighten the workload on Mondays or it is overwhelming. We are a 24/6 lab (no techs in lab Saturday after 3:30and until 0400 Mondays) Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Working Saturday
I applaud labs who have moved away from Saturday rotations. But I'm really at a loss to understand how it cuts costs? Don't most of you shift your work week for your staff if filling a Saturday rotation? So long as you don't pay OT for a Saturday, wouldn't the costs be the same? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Re: Working Saturday
We give a half day during the week for the half day worked on Saturday. This ended the overtime. Since we start at 4AM generally the person on call will have some day during the week they want to leave early. Since we have the schedule up before the first of the month most can make appointments or personal things for that half day. It cut down on time off during work hours and allowed us to only pay call pay if the person is needed during an overnight. Our shifts start at 4AM and we close at 4:30PM so they only cove the open hours. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Friday, October 24, 2014 10:52 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Working Saturday I applaud labs who have moved away from Saturday rotations. But I'm really at a loss to understand how it cuts costs? Don't most of you shift your work week for your staff if filling a Saturday rotation? So long as you don't pay OT for a Saturday, wouldn't the costs be the same? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Re: Working Saturday
You are right terry, it does not save in costs and just shift the workload. However, we staff one tech on Saturday to cover rush transplant bx That tech takes the previouis Monday off. Because we have to pay 3 hours minimum for any call back we simply staff it 8 hours. The Saturday tech embeds and cuts all the bx received Friday (100 to 150 blocks), does the same-day transplant rush, any Friday overnight rush, and load the processors with blocks from Saturday grossing (a catchup day for residents. ). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Friday, October 24, 2014 8:52 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Working Saturday I applaud labs who have moved away from Saturday rotations. But I'm really at a loss to understand how it cuts costs? Don't most of you shift your work week for your staff if filling a Saturday rotation? So long as you don't pay OT for a Saturday, wouldn't the costs be the same? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IFN gamma
Happy Friday!! I was wondering if the status of IHC staining for IFN gamma has changed since Chris Van Der Loos published the article back in 2001. Back then he stated that it was not possible to stain for IFN gamma via IHC. Immunohistochemical Detection of Interferon-gamma Fake or Fact? Chris M. van der Loos, Mischa A. Houtkamp, Onno J. de Boer, Peter Teeling, Allard C. van der Wal, and Anton E. Becker Academic Medical Center, Department of Cardiovascular Pathology, Amsterdam, The Netherlands Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.commailto:l...@premierlab.com www.premierlab.comhttp://www.premierlab.com/ March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryosectioning hydrogel
I am having a difficult time sectioning hydrogel @ 10u on the cryostat. Does anyone have a helpful suggestion? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ALK IHC
Recently all the pathologists I work with prefer the ALK FISH. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 23 Oct 2014 13:41:15 -0700 From: marktara...@gmail.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ALK IHC
Did you ever run ALK IHC on lung cancer cases or have they always used FISH? thanks Mark Tarango On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver joellewea...@hotmail.com wrote: Recently all the pathologists I work with prefer the ALK FISH. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 23 Oct 2014 13:41:15 -0700 From: marktara...@gmail.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ALK IHC
We are in the process of bringing this on-line. We are using a rabbit monoclonal (clone D5F3) from Cell Signaling Technologies (Danvers, MA). There are cases that are obviously positive and then there cases that are Equivocal (like HER2) where you need to do the FISH test. The advantages of the IHC are less cost, faster result, can be used on specimens with limited tumor cells present, and it may prove useful for those tumors that show genomic heterogeneity. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, October 23, 2014 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ALK IHC
We've always done FISH.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, October 24, 2014 3:49 PM To: Joelle Weaver Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ALK IHC Did you ever run ALK IHC on lung cancer cases or have they always used FISH? thanks Mark Tarango On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver joellewea...@hotmail.com wrote: Recently all the pathologists I work with prefer the ALK FISH. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 23 Oct 2014 13:41:15 -0700 From: marktara...@gmail.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ALK IHC
I can tell you that my biggest trouble in all dealing with ALK was finding enough positive cases. Joelle Weaver MAOM, HTL (ASCP) QIHC From: joyce.we...@emoryhealthcare.org To: marktara...@gmail.com; joellewea...@hotmail.com CC: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ALK IHC Date: Fri, 24 Oct 2014 20:29:42 + We've always done FISH.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, October 24, 2014 3:49 PM To: Joelle Weaver Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ALK IHC Did you ever run ALK IHC on lung cancer cases or have they always used FISH? thanks Mark Tarango On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver joellewea...@hotmail.com wrote: Recently all the pathologists I work with prefer the ALK FISH. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 23 Oct 2014 13:41:15 -0700 From: marktara...@gmail.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ALK IHC
I can tell you that my biggest trouble in all dealing with ALK was finding enough positive cases. Joelle Weaver MAOM, HTL (ASCP) QIHC From: joyce.we...@emoryhealthcare.org To: marktara...@gmail.com; joellewea...@hotmail.com CC: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ALK IHC Date: Fri, 24 Oct 2014 20:29:42 + We've always done FISH.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, October 24, 2014 3:49 PM To: Joelle Weaver Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ALK IHC Did you ever run ALK IHC on lung cancer cases or have they always used FISH? thanks Mark Tarango On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver joellewea...@hotmail.com wrote: Recently all the pathologists I work with prefer the ALK FISH. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 23 Oct 2014 13:41:15 -0700 From: marktara...@gmail.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ALK IHC
I think it is probably better with the rabbit monoclonal IHC than maybe in the past. IHC is MUCH cheaper, easier and faster than the FISH. Doing ALK FISH manually was easier than Her2 FISH manual, but still takes much more time than any IHC, and is expensive ( but necessary for certain cases). Seems like the IHC would be nice screen with a reflex to FISH. Joelle Weaver MAOM, HTL (ASCP) QIHC From: richard.car...@hhchealth.org To: marktara...@gmail.com; Histonet@lists.utsouthwestern.edu Date: Fri, 24 Oct 2014 20:15:24 + Subject: RE: [Histonet] ALK IHC CC: We are in the process of bringing this on-line. We are using a rabbit monoclonal (clone D5F3) from Cell Signaling Technologies (Danvers, MA). There are cases that are obviously positive and then there cases that are Equivocal (like HER2) where you need to do the FISH test. The advantages of the IHC are less cost, faster result, can be used on specimens with limited tumor cells present, and it may prove useful for those tumors that show genomic heterogeneity. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, October 23, 2014 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ALK IHC
I can tell you that my biggest trouble in all dealing with ALK was finding enough positive cases. Joelle Weaver MAOM, HTL (ASCP) QIHC From: joyce.we...@emoryhealthcare.org To: marktara...@gmail.com; joellewea...@hotmail.com CC: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ALK IHC Date: Fri, 24 Oct 2014 20:29:42 + We've always done FISH.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, October 24, 2014 3:49 PM To: Joelle Weaver Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ALK IHC Did you ever run ALK IHC on lung cancer cases or have they always used FISH? thanks Mark Tarango On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver joellewea...@hotmail.com wrote: Recently all the pathologists I work with prefer the ALK FISH. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 23 Oct 2014 13:41:15 -0700 From: marktara...@gmail.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ALK IHC
I can tell you that my biggest trouble in all dealing with ALK was finding enough positive cases. Joelle Weaver MAOM, HTL (ASCP) QIHC From: joyce.we...@emoryhealthcare.org To: marktara...@gmail.com; joellewea...@hotmail.com CC: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ALK IHC Date: Fri, 24 Oct 2014 20:29:42 + We've always done FISH.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, October 24, 2014 3:49 PM To: Joelle Weaver Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ALK IHC Did you ever run ALK IHC on lung cancer cases or have they always used FISH? thanks Mark Tarango On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver joellewea...@hotmail.com wrote: Recently all the pathologists I work with prefer the ALK FISH. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 23 Oct 2014 13:41:15 -0700 From: marktara...@gmail.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
