[Histonet] HistoPro 150 Embedding Center
Hi Everyone: Has anyone had experience with the Rushabh HistoPro 150 embedding center? Could you share your thoughts and opinions about this machine? Thank you, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Acetone fixation
We use acetone to denature fatty tumors without any problems. They sit in it for at least half an hour. I don't rinse them. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Saturday, November 15, 2014 2:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Acetone fixation Sheree H (where?) asks: Have questions about using acetone at the grossing counter. Does anyone have problems with it being carried over into the formalin on the processor? We rinse, open cassette to let it dry completely and even change the cassette. I've had issues with my processor and want to make sure it's not coming from the acetone. Acetone doesn't belong in the grossing area - too much of a fire and explosion hazard. If your pathologists are using it to fix marking ink onto specimens, they can use 3% acetic acid (half-strength white vinegar). I don't use anything at all - if you blot the specimen dry before you put the ink on, it'll stay put. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processors
We are looking to purchase a new tissue processor for our tissue core. The workload is quite light. Can anyone recommend a small, reliable processor ? Thank you, Valerie Ratliff BS HT(ASCP) Wayne State University Detroit, Michigan --- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Job Opportunity
Hi Histonetters!! Histology Tech position: Full time Monday thru Friday. Jasper, Indiana 47546 Jasper is a regional center in southwestern Indiana, noted for its German Catholic ancestral roots Jasper has often been called the Wood Capital of the World, boasting a large number of furniture companies, including Kimball Internationalhttp://en.wikipedia.org/wiki/Kimball_International and Masterbrand Cabinetshttp://en.wikipedia.org/wiki/Fortune_Brands. Jasper is also home to the Southern Indiana Education Service Center (SIEC), Jasper Engines Transmissions (largest remanufacturer in the market), and to a satellite campus of Vincennes Universityhttp://en.wikipedia.org/wiki/Vincennes_University. JOB SUMMARY: Responsible for embedding, sectioning and cover slipping paraffin blocks and staining tissues to provide the pathologist with accurately prepared specimens for review and diagnosis. The employee works with specimens from patients of all ages. The employee must reflect mission statement and philosophy of Memorial Hospital and Health Care Center in daily work habits and contacts. QUALIFICATIONS: Education: High school diploma or equivalent is required. Associate degree preferred. HT (ASCP) certification is preferred. Three years' experience as histology assist is acceptable. Training: Six months on the job training required. Experience: Previous histology experience is preferred. New graduates of histology school will be considered. S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbald...@mhhcc.orgmailto:sbald...@mhhcc.org Christ's healing mission of compassion empowers us to be for others through Quality and Excellence ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Supervisor job opening near Buffalo, NY
We are currently recruiting for a client in the Buffalo, NY area for a Histology Supervisor with a valid NY lab license and 6 years of experience including experience as a leader. Interested candidates please forward an updated resume to bran...@alliedsearchpartners.com for review. To view a complete list of Allied Search Partners current openings go to: http://www.jobs.net/jobs/alliedsearchpartners/en-us/ http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 F: 888.388.7572 http://www.jobs.net/jobs/alliedsearchpartners/join?joinpath=Exportablejoin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ER, PR, HER2 CARGES
Hi Guys, Please can you guys tell me the CPT Codes for ER, PR and HER2? Thanks for your anticipated cooperation. Thanks, Banjo == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TRAP staining protocol troubleshooting
Hello histonetter heroes! I have an issue with a TRAP staining protocol that I'm currently using that involves Fast Red Violet LB Salt for the staining of the Osteoclast and then Fast Green as a counterstain. The osteoclast are suppose to stain Red Violet and the background is suppose to stain green. However, I'm not seeing any Red Violet Osteoclast but just really really Green tissue. The bone I was using was cortical bone but surely there would at least 1 Osteoclast in all of sections that I had. Here is the protocol that I'm using: Trap Staining solution Mix: Trap Basic Incubation medium, Fast Red Violet LB Salt, and Naphthol AS-MX Phophaget substrate mix Procedure: 1. Place TRAP staining solution mix in staining dish and pre-warm to 37 degree celsius in waterbath 2. Deparafinnize slides and rehydrate through graded ethanols to distilled water. 3. Place slides in pre-warmed TRAP staining solution mix and incubate at 37 celsius for 30 mins or until control is developed. 4. Rinse in distilled wtaer 5. Counterstain with 0.02% Fast Green for 30 seconds and rinse quickly in distilled water. 6. Dehydrate quickly thorugh graded alcohols, 5 seconds each, clear in Xylene and mount. The problem is that the main staining with the Fast Red is not very substantial.at least not as much as it should be. When I take the slide from the water bath, the tissue section is only slightly red...and may even look more orangish yellowwhich is a long ways away from red violet. Then when I counterstain with Fast Green, I feel that the Fast Green stain is so prominent that it just superimposes the Fast Red stain. For anyone that has experience with TRAP staining or Fast Red Violet staining then that would be awesome to hear your input! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Decalcified Bone Clearing time
Hey everyone! I'm having some issues about clearing the bone tissues after they get done dehydrating from decalcification. I know that over clearing the bone tissues in Xylene for too long can cause them to become very brittle and I'm afraid that that was what was happening. The bone blocks are not very bigtheir weights are listed as 0.222g, 0.100g, and 0.052g. The biggest bone block is about 4mmx4mmx4mm so it's not very big I would say. I cleared with 50% Ethanol/50% Xylene for 1 hour, and then I changed it with 100% Xylene for another hour. The bone blocks become clear as expected but they begin to get a yellowish-tan tinge to them. I'm not the most experienced but from what I've studied, I don't think that 2 hours in Xylene is overkill by any means so maybe it's something that I'm doing subconsciously.if anyone has any suggestions are ideas please let me know! :) Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone Marrow processing using Immunocal
I am looking for a Bone Marrow procedure that uses Immunocal as the decalcifier. I am particularly interested in how long they fix in Immunocal and if heat is used in any way . Our lab just started using this product because we are under the impression that it gave better ISH results. Thank-you all so much for any assistance. I really appreciate everyone's help. Carolyn K. Barnes Histology Supervisor McGuire VA Medical Center Richmond, VA 23249 Ph: 804-675-5000, x2158 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Position Available - Field Support Scientist: Molecular
Hi All, This is an exclusively retained opportunity with a world leader in cancer diagnostics. Our client has acquired a company that specializes in molecular FISH probes, and they have recently opened an opportunity on the West Coast for a Field Support Scientist. The ideal candidate will be currently working in a Cytogenetics Laboratory and will have extensive FISH experience. This is a customer facing role with a lot of growth opportunity. The ideal location is anywhere on the West Coast The company offers a strong package including a base salary, competitive commission structure, company car, gas card, cell phone, laptop computer, and full benefits. If you or anyone you know many be interested in learning more about this position, please contact me directly at d...@personifysearch.com Thank you so much, Danielle Robinson Talent Management Executive 5020 Weston Parkway, Ste 315 Cary, NC 27513 US Toll Free: +1 (800) 875-6188 ext. 140 International Access Phone: +1.919.473.3762 http://www.personifysearch.com/ http://www.linkedin.com/pub/danielle-robinson/23/5aa/70a ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laser Capture Microdissection Instrument
We are in the process of purchasing a LCM system, I'm interested in your opinions of Leica and Arcturus. Regards, Mary Ann Clements, B.S. Biorepository Manager Eastern Virginia Medical School Leroy T. Canoles Jr. Cancer Research Center Lewis Hall, Room 3018 700 West Olney Road Norfolk, VA 23501 email: cleme...@evms.edumailto:cleme...@evms.edu phone: (757) 446-7910 fax: (757) 446- 7059 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MOHS Technician Job in Arizona
Hello, I have a MOHS Technician Job in Arizona for Full Time/Direct Hire. Please message me for more details and the job description. Thank you, Melissa Owens (Phelan) Allied Search Partners ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TRAP staining protocol troubleshooting
Actually trap will work if you pre incubate it in 0.2M Tris buffer Ph9 (use sodium hydroxide to pH) for 1 hour at 37dc then proceed with the trap stain without rinsing. Check the stain in 20 min as it develops quickly after this. This protocol was given to me by the late great Hermina and it works really well even on formic acid decaled FFPE bone in my hands. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net From: prueg...@hotmail.com To: wa...@livemail.uthscsa.edu Subject: RE: [Histonet] TRAP staining protocol troubleshooting Date: Mon, 17 Nov 2014 13:22:24 -0700 Trap dose not work on decalcified bone unless you have decaled with EDTA in my experience. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net From: wa...@livemail.uthscsa.edu To: histonet@lists.utsouthwestern.edu Date: Mon, 17 Nov 2014 18:58:50 + Subject: [Histonet] TRAP staining protocol troubleshooting Hello histonetter heroes! I have an issue with a TRAP staining protocol that I'm currently using that involves Fast Red Violet LB Salt for the staining of the Osteoclast and then Fast Green as a counterstain. The osteoclast are suppose to stain Red Violet and the background is suppose to stain green. However, I'm not seeing any Red Violet Osteoclast but just really really Green tissue. The bone I was using was cortical bone but surely there would at least 1 Osteoclast in all of sections that I had. Here is the protocol that I'm using: Trap Staining solution Mix: Trap Basic Incubation medium, Fast Red Violet LB Salt, and Naphthol AS-MX Phophaget substrate mix Procedure: 1. Place TRAP staining solution mix in staining dish and pre-warm to 37 degree celsius in waterbath 2. Deparafinnize slides and rehydrate through graded ethanols to distilled water. 3. Place slides in pre-warmed TRAP staining solution mix and incubate at 37 celsius for 30 mins or until control is developed. 4. Rinse in distilled wtaer 5. Counterstain with 0.02% Fast Green for 30 seconds and rinse quickly in distilled water. 6. Dehydrate quickly thorugh graded alcohols, 5 seconds each, clear in Xylene and mount. The problem is that the main staining with the Fast Red is not very substantial.at least not as much as it should be. When I take the slide from the water bath, the tissue section is only slightly red...and may even look more orangish yellowwhich is a long ways away from red violet. Then when I counterstain with Fast Green, I feel that the Fast Green stain is so prominent that it just superimposes the Fast Red stain. For anyone that has experience with TRAP staining or Fast Red Violet staining then that would be awesome to hear your input! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Decalcified Bone Clearing time
Make sure that you fix well in formalin before you decal (what are you using?), then I rinse off the decal solution (I use formic acid) and put them back into formalin before putting on the tissue processor for a longer than usual schedule and especially give them longer time to infiltrate in paraffin, at least 3 changes for 2 hours each after dehydration and clearing in xylene. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net From: wa...@livemail.uthscsa.edu To: histonet@lists.utsouthwestern.edu Date: Mon, 17 Nov 2014 19:07:42 + Subject: [Histonet] Decalcified Bone Clearing time Hey everyone! I'm having some issues about clearing the bone tissues after they get done dehydrating from decalcification. I know that over clearing the bone tissues in Xylene for too long can cause them to become very brittle and I'm afraid that that was what was happening. The bone blocks are not very bigtheir weights are listed as 0.222g, 0.100g, and 0.052g. The biggest bone block is about 4mmx4mmx4mm so it's not very big I would say. I cleared with 50% Ethanol/50% Xylene for 1 hour, and then I changed it with 100% Xylene for another hour. The bone blocks become clear as expected but they begin to get a yellowish-tan tinge to them. I'm not the most experienced but from what I've studied, I don't think that 2 hours in Xylene is overkill by any means so maybe it's something that I'm doing subconsciously.if anyone has any suggestions are ideas please let me know! :) Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bone Marrow processing using Immunocal
I use a platform shaker and slosh the sample around while it is in decal solution at RT, just make sure it is well fixed before you decal, Immunocal is 5% formic acid decal solution it is not a fixative and it is a bit slower than some of the rapid decal reagents out there but is much better for IHC. When I was in HemePath we would fix for about 6 hrs before decaling on the shaker for 2-4hrs depending on the size (thickness) of the bone bx. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net Date: Mon, 17 Nov 2014 14:05:28 -0500 From: carolyn.barn...@va.gov To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] Bone Marrow processing using Immunocal I am looking for a Bone Marrow procedure that uses Immunocal as the decalcifier. I am particularly interested in how long they fix in Immunocal and if heat is used in any way . Our lab just started using this product because we are under the impression that it gave better ISH results. Thank-you all so much for any assistance. I really appreciate everyone's help. Carolyn K. Barnes Histology Supervisor McGuire VA Medical Center Richmond, VA 23249 Ph: 804-675-5000, x2158 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] TRAP staining protocol troubleshooting
That's interesting because I buffered it to 4.7-5.0..that's actually a huge difference.does that have to do with he Fast Red approach? Sent from my iPhone On Nov 17, 2014, at 2:40 PM, Patsy Ruegg prueg...@hotmail.commailto:prueg...@hotmail.com wrote: Actually trap will work if you pre incubate it in 0.2M Tris buffer Ph9 (use sodium hydroxide to pH) for 1 hour at 37dc then proceed with the trap stain without rinsing. Check the stain in 20 min as it develops quickly after this. This protocol was given to me by the late great Hermina and it works really well even on formic acid decaled FFPE bone in my hands. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.commailto:prueg...@hotmail.com pru...@ihctech.netmailto:pru...@ihctech.net From: prueg...@hotmail.commailto:prueg...@hotmail.com To: wa...@livemail.uthscsa.edumailto:wa...@livemail.uthscsa.edu Subject: RE: [Histonet] TRAP staining protocol troubleshooting Date: Mon, 17 Nov 2014 13:22:24 -0700 Trap dose not work on decalcified bone unless you have decaled with EDTA in my experience. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.commailto:prueg...@hotmail.com pru...@ihctech.netmailto:pru...@ihctech.net From: wa...@livemail.uthscsa.edumailto:wa...@livemail.uthscsa.edu To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Date: Mon, 17 Nov 2014 18:58:50 + Subject: [Histonet] TRAP staining protocol troubleshooting Hello histonetter heroes! I have an issue with a TRAP staining protocol that I'm currently using that involves Fast Red Violet LB Salt for the staining of the Osteoclast and then Fast Green as a counterstain. The osteoclast are suppose to stain Red Violet and the background is suppose to stain green. However, I'm not seeing any Red Violet Osteoclast but just really really Green tissue. The bone I was using was cortical bone but surely there would at least 1 Osteoclast in all of sections that I had. Here is the protocol that I'm using: Trap Staining solution Mix: Trap Basic Incubation medium, Fast Red Violet LB Salt, and Naphthol AS-MX Phophaget substrate mix Procedure: 1. Place TRAP staining solution mix in staining dish and pre-warm to 37 degree celsius in waterbath 2. Deparafinnize slides and rehydrate through graded ethanols to distilled water. 3. Place slides in pre-warmed TRAP staining solution mix and incubate at 37 celsius for 30 mins or until control is developed. 4. Rinse in distilled wtaer 5. Counterstain with 0.02% Fast Green for 30 seconds and rinse quickly in distilled water. 6. Dehydrate quickly thorugh graded alcohols, 5 seconds each, clear in Xylene and mount. The problem is that the main staining with the Fast Red is not very substantial.at least not as much as it should be. When I take the slide from the water bath, the tissue section is only slightly red...and may even look more orangish yellowwhich is a long ways away from red violet. Then when I counterstain with Fast Green, I feel that the Fast Green stain is so prominent that it just superimposes the Fast Red stain. For anyone that has experience with TRAP staining or Fast Red Violet staining then that would be awesome to hear your input! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PgR Negative Cases for 2014
Did anyone else see an increase of PgR Negative cases early in 2014? Seems like there was an increase in the % of cases that were ER + but PgR negative. We saw an increase in cases particularly from Jan to Jun30th. Any thoughts? Thanks in advance, Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] competency checklist
How are laboratories handling the competency checklist? Do you have one list that is all inclusive or blank forms and fill in with the competency observed? Thanks, Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
