[Histonet] Rubber mats for pinning specimens
Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something we can cut to fit our current containers, no small specimen pans needed. The pathologists have also stated that they do NOT want cork. Thanks again, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Rubber mats for pinning specimens
Have you tried to pin on large cork sheets? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, November 18, 2014 8:18 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Rubber mats for pinning specimens Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something we can cut to fit our current containers, no small specimen pans needed. The pathologists have also stated that they do NOT want cork. Thanks again, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Rubber mats for pinning specimens
We make up paraffin trays, either in a cafeteria tray (sssh) or in the lid of a slide box if it's for something small. Works like a charm and supplies are at hand. Besides that, paraffin floats in a formalin tank as well. You could have custom cut trays and still use your paraffin. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, November 18, 2014 10:21 AM To: Dennis Hahn; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Rubber mats for pinning specimens Have you tried to pin on large cork sheets? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, November 18, 2014 8:18 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Rubber mats for pinning specimens Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something we can cut to fit our current containers, no small specimen pans needed. The pathologists have also stated that they do NOT want cork. Thanks again, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Rubber mats for pinning specimens
We use Styrofoam and break it to the right size. Get them out of shipping boxes. Free! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Tuesday, November 18, 2014 10:25 AM To: Bea DeBrosse-Serra; Dennis Hahn; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Rubber mats for pinning specimens We make up paraffin trays, either in a cafeteria tray (sssh) or in the lid of a slide box if it's for something small. Works like a charm and supplies are at hand. Besides that, paraffin floats in a formalin tank as well. You could have custom cut trays and still use your paraffin. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, November 18, 2014 10:21 AM To: Dennis Hahn; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Rubber mats for pinning specimens Have you tried to pin on large cork sheets? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, November 18, 2014 8:18 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Rubber mats for pinning specimens Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something we can cut to fit our current containers, no small specimen pans needed. The pathologists have also stated that they do NOT want cork. Thanks again, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] tissue tek 5 embedding center
Can anyone tell me if the Tissue Tek 5 has a work light? Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.orgmailto:hor...@archildrens.org archildrens.orghttp://www.archildrens.org/ ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome ergonimic evaluation
Has anyone ever had an ergonomic evaluation specific to their microtome station? If so, could you please give me the contact information of the evaluator? Thanks, Kristina Wyatt, M.A. Supervisor: Histopathology/Immunohistochemistry L216 Mosier Hall Kansas State Veterinary Diagnostic Laboratory 785-532-4464 kdwy...@vet.k-state.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Flames at embedding centers
Is anyone out there still using flames at the embedding centers for their forceps? If not, what are you using instead? Pat Ludlow Technical Specialist/Supervisor Histology HRLMP 905-522-1155 X35954 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Flames at embedding centers
Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. If you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, there are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination. Cotton applicator swabs work great for this. And always keep a towel or gauze handy to wipe the tips of the forceps between specimens. Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Flames at embedding centers
Grinning and remembering the good old days. What's more fun is the look of horror on the faces of the young ones when they hear it! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. If you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, there are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination. Cotton applicator swabs work great for this. And always keep a towel or gauze handy to wipe the tips of the forceps between specimens. Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Flames at embedding centers
Like! ditto! Paula Pierce,BS,HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com From: Blazek, Linda lbla...@digestivespecialists.com To: Jay Lundgren jaylundg...@gmail.com; Ludlow Patricia lud...@hhsc.ca Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, November 18, 2014 3:03 PM Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the good old days. What's more fun is the look of horror on the faces of the young ones when they hear it! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. If you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, there are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination. Cotton applicator swabs work great for this. And always keep a towel or gauze handy to wipe the tips of the forceps between specimens. Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Flames at embedding centers
The open flames were great because they incinerated any residual tissue fragments, like from EMCs. Hardy ever had cross contamination back in those days. Lab fires, yes - I remember one in the late 1960's at St. Elizabeth Hospital in Chicago. (Just heard about it, wasn't there at the time) Alcohol lamp ignited xylene in the lab on the 8th floor. Bad story. I can see the reason to not have open flames anymore. We used loop incinerators from the Bac-T lab for awhile, too, instead of open flames. Jackie O' -Original Message- From: Blazek, Linda lbla...@digestivespecialists.com To: Jay Lundgren jaylundg...@gmail.com; Ludlow Patricia lud...@hhsc.ca Cc: histonet histonet@lists.utsouthwestern.edu Sent: Tue, Nov 18, 2014 3:04 pm Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the good old days. What's more fun is the look of orror on the faces of the young ones when they hear it! -Original Message- rom: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] n Behalf Of Jay Lundgren ent: Tuesday, November 18, 2014 2:42 PM o: Ludlow Patricia c: histonet@lists.utsouthwestern.edu ubject: Re: [Histonet] Flames at embedding centers Ahh, the good old days of walking into the lab and lighting all the Bunsen urners first thing in the morning. I didn't have any hair on my knuckles for ears. Embedding with a Coke on the cold plate, and a smoke in the ashtray next o you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. f you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, here are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to revent cross contamination. Cotton applicator swabs work great for this. And lways keep a towel or gauze handy to wipe the tips of the forceps between pecimens. Forceps warmers unfortunately don't incinerate any stray tissue like Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Flames at embedding centers
Yes, the good old days!And, we survived! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children’s Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, November 18, 2014 3:14 PM To: Blazek, Linda; Jay Lundgren; Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Like! ditto! Paula Pierce,BS,HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com From: Blazek, Linda lbla...@digestivespecialists.com To: Jay Lundgren jaylundg...@gmail.com; Ludlow Patricia lud...@hhsc.ca Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, November 18, 2014 3:03 PM Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the good old days. What's more fun is the look of horror on the faces of the young ones when they hear it! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. If you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, there are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination. Cotton applicator swabs work great for this. And always keep a towel or gauze handy to wipe the tips of the forceps between specimens. Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Flames at embedding centers
We're well preserved! -Original Message- From: Horn, Hazel V [mailto:hor...@archildrens.org] Sent: Tuesday, November 18, 2014 4:23 PM To: 'Paula Pierce'; Blazek, Linda; Jay Lundgren; Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Flames at embedding centers Yes, the good old days!And, we survived! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children’s Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, November 18, 2014 3:14 PM To: Blazek, Linda; Jay Lundgren; Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Like! ditto! Paula Pierce,BS,HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com From: Blazek, Linda lbla...@digestivespecialists.com To: Jay Lundgren jaylundg...@gmail.com; Ludlow Patricia lud...@hhsc.ca Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, November 18, 2014 3:03 PM Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the good old days. What's more fun is the look of horror on the faces of the young ones when they hear it! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. If you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, there are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination. Cotton applicator swabs work great for this. And always keep a towel or gauze handy to wipe the tips of the forceps between specimens. Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Flames at embedding centers
We have days when one of the histologist who has been here for years and I (going in 50years) start talking about the good days and everyone here is under 40. They look shocked and then disbelief and then they think we are kidding. They are so sure we have always known which chemicals were dangerous or killers and no one could possibly ever have been so careless. WOW! I like to show them old equipment and ask how they think it might work in a lecture on tissue processing. They have no clue about an open processor in small room with no ventilation and we worked in it with our open stains lines and flames on the counter. Now it does sound scary! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, November 18, 2014 3:04 PM To: Jay Lundgren; Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the good old days. What's more fun is the look of horror on the faces of the young ones when they hear it! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. If you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, there are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination. Cotton applicator swabs work great for this. And always keep a towel or gauze handy to wipe the tips of the forceps between specimens. Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Flames at embedding centers
My very first Histo job was with Metpath in Hackensack NJ. We had Bunsen burners right next to a xylene bath for dirty molds. One day a tech accidentally had a xylene Bunsen burner collision and started a fire. We had another tech who was quick with head and feet who was able to extinguish it - but there was damage. As to smoking in the lab I do remember. Between the fumes and the smoke I found breathing a little difficult. But yes those were the good 'ol days. Vikki On Nov 18, 2014 5:22 PM, Marcum, Pamela A pamar...@uams.edu wrote: We have days when one of the histologist who has been here for years and I (going in 50years) start talking about the good days and everyone here is under 40. They look shocked and then disbelief and then they think we are kidding. They are so sure we have always known which chemicals were dangerous or killers and no one could possibly ever have been so careless. WOW! I like to show them old equipment and ask how they think it might work in a lecture on tissue processing. They have no clue about an open processor in small room with no ventilation and we worked in it with our open stains lines and flames on the counter. Now it does sound scary! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, November 18, 2014 3:04 PM To: Jay Lundgren; Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the good old days. What's more fun is the look of horror on the faces of the young ones when they hear it! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. If you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, there are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination. Cotton applicator swabs work great for this. And always keep a towel or gauze handy to wipe the tips of the forceps between specimens. Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] double staining alkaline phosphatase
Dear Histonetters, Is it technically possible to perform a double staining using alkaline phosphatase in both stainings ? I was thinking to perform a seqeuntial staining and develop the staining for the first protein and detect with a blue AP substrate and then perform staining for the second protein and detect with a red AP substrate. However, after the first staining, I would need to be able to completely block AP-activity or circumvent cross-reaction in some way. Yves ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
