[Histonet] Staining for plant peroxidase

2014-11-24 Thread Hans B Snyder
Hello All,

We are in need of help finding sources for plant peroxidase staining.  If
someone could point me in the right direction, a link, papers or books, I
am happy to research.

Thank you


Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com ha...@histologistics.com
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[Histonet] HER2 scoring exercises for breast cases

2014-11-24 Thread Burnett, Brandy
Does anyone know of a good website for practicing HER2 scoring on breast tissue?

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[Histonet] N-Cadherin Antibody for FFPE IHC

2014-11-24 Thread Rachel Gonzalez
Hi 

 

I just joined the histonet (3 days). so I am not sure of the appropriateness
of this question but I would really appreciate the help.  

 

Does anyone know of an N-cadherin antibody that works on paraffin embedded
tissue. I have a deadline in the next few weeks to get the staining done. We
have one in house that is working beautifully but the project requires a
second N-Cadherin antibody.

 

For the second one I have tried Spring Biosciences N-Cadherin M3900 and I am
getting no staining (Kidney, Carcinoid, Glioma).  I used Spring Bioscience
protocol exactly and 9 variations of their protocol. I used a titer of 1:10
and all I get is background staining in mostly fluids in vessels, cysts and
tubules. There is no  membrane staining that the other antibody is giving.
Talking to Spring Biosciences the customer service person (Jennifer W)
stated this antibody only works on mesothelioma. I am not sure that I want
an antibody that only works on one tissue especially since my goal is to
stain carcinoid.

 

I was hoping for a few recommendations to help narrow the scope.

 

Thanks,

RMG

 

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[Histonet] RE: Specimen numbering systems

2014-11-24 Thread hymclab
We do it the same as Toni and from the CAP Uniform Labeling of slides and 
Blocks presentation I attended at NSH that is what CAP came up with for uniform 
labeling (S14-1234 A1, A2, A3, B1, B2, etc...).



Dawn Schneider, HT(ASCP)
Howard Young Medical Center
240 Maple Ave.
Woodruff, WI 54568
715-356-8174




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, November 21, 2014 2:18 PM
To: 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

We use a numeric for the accession number (S-14-1234), followed by alpha for 
the specimen(A, B, C), and within that have numeric for the number of blocks in 
each specimen (A1, A2, A3). We haven't ever gone to Z in our current LIS, and 
the ability to add unlimited blocks to each specimen makes it easy to deal with.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Friday, November 21, 2014 3:11 PM
To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

We are currently using Numeric for the specimen (or part) and Alpha for the 
block.  I don't like it; we frequently have 1Z blocks for large CA 
resections!  I would like to change this going forward.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G.
Sent: Friday, November 21, 2014 2:03 PM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Specimen numbering systems

Can large facilities of more than 500 beds please let me know how they are 
numbering their Surgical specimens.  Alpha for the Specimens and numeric for 
the Block or Numeric for the Specimen and Alpha for the Block.

Thanks,

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager

Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com


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[Histonet] RE: Specimen numbering systems

2014-11-24 Thread Roy, Ryan
I agree with Dawn . Basically use a numeric for the Block and an Alpha for the 
specimen. This way a large resection specimen would receive an A and all blocks 
for said specimen would be labeled as A1,A2,A3...ect 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Monday, November 24, 2014 2:26 PM
To: 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [EXTERNAL] [Histonet] RE: Specimen numbering systems

We do it the same as Toni and from the CAP Uniform Labeling of slides and 
Blocks presentation I attended at NSH that is what CAP came up with for uniform 
labeling (S14-1234 A1, A2, A3, B1, B2, etc...).



Dawn Schneider, HT(ASCP)
Howard Young Medical Center
240 Maple Ave.
Woodruff, WI 54568
715-356-8174




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, November 21, 2014 2:18 PM
To: 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

We use a numeric for the accession number (S-14-1234), followed by alpha for 
the specimen(A, B, C), and within that have numeric for the number of blocks in 
each specimen (A1, A2, A3). We haven't ever gone to Z in our current LIS, and 
the ability to add unlimited blocks to each specimen makes it easy to deal with.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Friday, November 21, 2014 3:11 PM
To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

We are currently using Numeric for the specimen (or part) and Alpha for the 
block.  I don't like it; we frequently have 1Z blocks for large CA 
resections!  I would like to change this going forward.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G.
Sent: Friday, November 21, 2014 2:03 PM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Specimen numbering systems

Can large facilities of more than 500 beds please let me know how they are 
numbering their Surgical specimens.  Alpha for the Specimens and numeric for 
the Block or Numeric for the Specimen and Alpha for the Block.

Thanks,

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager

Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com


**
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are prohibited without proper authorization. If you are not the intended 
recipient (or have received this e-mail in error) please notify the sender 
immediately and destroy this e-mail. Any unauthorized copying, disclosure or 
distribution of the material in this e-mail is strictly forbidden and possibly 
a violation of federal or state law and regulations. Baylor Health Care System, 
its subsidiaries, and affiliates hereby claim all applicable privileges related 
to this information.
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[Histonet] Re: Histonet Digest, Vol 132, Issue 26

2014-11-24 Thread Shaughnessy, Mary
thanks for the heads up. Let me look into this.

Mary Morrow (Shaughnessy)
Southwest Regional
Business Development Manager
General Data Healthcare
mshaughne...@general-data.com
480-291-2757
Sent from my iPad

 On Nov 24, 2014, at 11:02 AM, histonet-requ...@lists.utsouthwestern.edu 
 histonet-requ...@lists.utsouthwestern.edu wrote:

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 When replying, please edit your Subject line so it is more specific
 than Re: Contents of Histonet digest...


 Today's Topics:

   1. CPT code question (Pam DeFazio)
   2. Re: CPT code question (Jeryl Baker)
   3. RE: Intestinal mucus -- Carnoy or what? (Tony Henwood (SCHN))
   4. Staining for plant peroxidase (Hans B Snyder)


 --

 Message: 1
 Date: Sun, 23 Nov 2014 13:19:45 -0500
 From: Pam DeFazio pdefazio...@gmail.com
 Subject: [Histonet] CPT code question
 To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
 Message-ID:
cao_4wqhq9hhqby9vwll1r3wymvqs1bxljv0xkcqd81gt0rj...@mail.gmail.com
 Content-Type: text/plain; charset=UTF-8

 We use a nail softening solution to soften toenails submitted for possible
 fungus. Is there a CPT code for this or can I use the decalcification code?
 Thanks! Pam
 ARMC
 Athens, Ga


 --

 Message: 2
 Date: Sun, 23 Nov 2014 13:05:12 -0600
 From: Jeryl Baker jeribake...@gmail.com
 Subject: Re: [Histonet] CPT code question
 To: Pam DeFazio pdefazio...@gmail.com
 Cc: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
 Message-ID: 451e1aa4-b46c-4696-95b7-27fe94c85...@gmail.com
 Content-Type: text/plain;charset=us-ascii

 FYI Pam, hydrogen peroxide works great, if you soak the nail in that prior to 
 embedding, face the block and then soak the block prior to sectioning.

 Sent from my iPhone

 On Nov 23, 2014, at 12:19 PM, Pam DeFazio pdefazio...@gmail.com wrote:

 We use a nail softening solution to soften toenails submitted for possible
 fungus. Is there a CPT code for this or can I use the decalcification code?
 Thanks! Pam
 ARMC
 Athens, Ga
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 --

 Message: 3
 Date: Sun, 23 Nov 2014 22:22:04 +
 From: Tony Henwood (SCHN) tony.henw...@health.nsw.gov.au
 Subject: RE: [Histonet] Intestinal mucus -- Carnoy or what?
 To: 'Mikael Niku' mikael.n...@helsinki.fi,
histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
 Message-ID: 6d6bd1de8a5571489398b392a38a7157f53cc...@xmdb04.nch.kids
 Content-Type: text/plain; charset=us-ascii

 Since the mucus will tend to be water soluble. I would suggest 10% 
 formaldehyde in alcohol.
 Fix for the usual time, dependant on size.

 Regards
 Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
 Laboratory Manager  Senior Scientist, the Children's Hospital at Westmead
 Adjunct Fellow, School of Medicine, University of Western Sydney
 Tel: 612 9845 3306
 Fax: 612 9845 3318
 Pathology Department
 the children's hospital at westmead
 Cnr Hawkesbury Road and Hainsworth Street, Westmead
 Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mikael Niku
 Sent: Friday, 21 November 2014 9:23 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Intestinal mucus -- Carnoy or what?

 Hello!

 What is the best way to fix intestinal samples in order to preserve the mucus 
 (and the embedded bacteria)?
 I was recommended Carnoy's, but are there any alternatives... perhaps without 
 chloroform?

 With best regards,
 Mikael

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 although no computer viruses were detected, The Sydney Childrens Hospital's 

[Histonet] Kidney bx transport solution

2014-11-24 Thread Moe, Barbi A
Hi all -



Currently our histology lab staff travels to our Interventional Radiology suite 
when a kidney bx is performed.

At the procedure site, the sample is handed directly to the tech, it is 
evaluated for adequacy, divided, and put into the following solutions - 
formalin, Trump's, and Zeus Wash solution.  Upon return to the laboratory, the 
cores in Zeus Wash solution are then frozen for immunofluoresence done on site.



We are looking into a new way of doing things :-) and considering having the 
kidney bx delivered to the laboratory after procurement.  The samples would be 
hand-delivered to the lab immediately (within 5 - 10 minutes) and the samples 
(usually 3-4 cores) would be divided in the histology lab.



Could I get an idea of how many labs have the sample delivered to them vs the 
number that assist at the procedure site?



Also, any thoughts on what the best transport media/method would be?



All thoughts are much appreciated!



Barb Moe

Gundersen Lutheran Medical Center

La Crosse WI

ba...@gundersenhealth.orgmailto:ba...@gundersenhealth.org





Barb Moe
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[Histonet] RE: Kidney bx transport solution

2014-11-24 Thread Morken, Timothy
Barb, the clinical/nursing staff deliver the bx to our Grossing lab. Grossing 
takes some for light microscopy and forwards the parts  for IF and EM to the 
IF/EM lab.(we pick up from grossing or they deliver to us). In EM/If we look at 
the cores and determine the best dissection to make and freeze/fix there. If 
grossing has a hard time deciding if the cores are good they bring all the 
specimens to us and we figure it out. 

Everything comes fresh from the clinician on saline-wetted  telfa pads in a 
45mm petri dish in an ice container. We make up kits in the EM lab, including 
100ml plastic beakers of ice, and they use those kits for the biopsy retrieval. 
We don't use Zeus for in-house specimens (though we may for some future cases 
due to a new facility opening that is 30 to 45 min away by courier).

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for 
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message.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Moe, Barbi A
Sent: Monday, November 24, 2014 12:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Kidney bx transport solution

Hi all -



Currently our histology lab staff travels to our Interventional Radiology suite 
when a kidney bx is performed.

At the procedure site, the sample is handed directly to the tech, it is 
evaluated for adequacy, divided, and put into the following solutions - 
formalin, Trump's, and Zeus Wash solution.  Upon return to the laboratory, the 
cores in Zeus Wash solution are then frozen for immunofluoresence done on site.



We are looking into a new way of doing things :-) and considering having the 
kidney bx delivered to the laboratory after procurement.  The samples would be 
hand-delivered to the lab immediately (within 5 - 10 minutes) and the samples 
(usually 3-4 cores) would be divided in the histology lab.



Could I get an idea of how many labs have the sample delivered to them vs the 
number that assist at the procedure site?



Also, any thoughts on what the best transport media/method would be?



All thoughts are much appreciated!



Barb Moe

Gundersen Lutheran Medical Center

La Crosse WI

ba...@gundersenhealth.orgmailto:ba...@gundersenhealth.org





Barb Moe
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[Histonet] RE: Specimen numbering systems

2014-11-24 Thread Pathology-Histology Sr. Supervisor
I also agree with Dawn. We shall be start from Jan 2015 as this way.

Muhammad Tahseen, MT (JIMTEF) Japan
Senior supervisor Histopathology
SKMCHRC Lahore
Pakistan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roy, Ryan
Sent: Tuesday, November 25, 2014 12:42 AM
To: 'hymclab'; 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

I agree with Dawn . Basically use a numeric for the Block and an Alpha for the 
specimen. This way a large resection specimen would receive an A and all blocks 
for said specimen would be labeled as A1,A2,A3...ect 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Monday, November 24, 2014 2:26 PM
To: 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [EXTERNAL] [Histonet] RE: Specimen numbering systems

We do it the same as Toni and from the CAP Uniform Labeling of slides and 
Blocks presentation I attended at NSH that is what CAP came up with for uniform 
labeling (S14-1234 A1, A2, A3, B1, B2, etc...).



Dawn Schneider, HT(ASCP)
Howard Young Medical Center
240 Maple Ave.
Woodruff, WI 54568
715-356-8174




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, November 21, 2014 2:18 PM
To: 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

We use a numeric for the accession number (S-14-1234), followed by alpha for 
the specimen(A, B, C), and within that have numeric for the number of blocks in 
each specimen (A1, A2, A3). We haven't ever gone to Z in our current LIS, and 
the ability to add unlimited blocks to each specimen makes it easy to deal with.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Friday, November 21, 2014 3:11 PM
To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

We are currently using Numeric for the specimen (or part) and Alpha for the 
block.  I don't like it; we frequently have 1Z blocks for large CA 
resections!  I would like to change this going forward.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G.
Sent: Friday, November 21, 2014 2:03 PM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Specimen numbering systems

Can large facilities of more than 500 beds please let me know how they are 
numbering their Surgical specimens.  Alpha for the Specimens and numeric for 
the Block or Numeric for the Specimen and Alpha for the Block.

Thanks,

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager

Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com


**
This e-mail may contain confidential and/or privileged information. This 
information is intended only for the use of the individual(s) and entity(ies) 
to whom it is addressed. If you are the intended recipient, further disclosures 
are prohibited without proper authorization. If you are not the intended 
recipient (or have received this e-mail in error) please notify the sender 
immediately and destroy this e-mail. Any unauthorized copying, disclosure or 
distribution of the material in this e-mail is strictly forbidden and possibly 
a violation of federal or state law and regulations. Baylor Health Care System, 
its subsidiaries, and affiliates hereby claim all applicable privileges related 
to this information.
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[Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

2014-11-24 Thread Arun Jyothi S.P
Dear all
After processing in vip 6 the last paraffin has a strong odour of formalin

Have anybody experienced the same
Any ideas why it is happening.

Arun
Kuwait
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