RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

2014-11-25 Thread Jamal
Hi
If the paraffin are overheated it gives abnormal odor.
Recheck the paraffin bath temperature and the paraffin melting point then make 
sure to adjust the bath temperature at 2 degrees above the melting point.
Please update me.


Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg Medical 
Laboratories |  Mobile +966 503629832| j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: 
+966 12 670 0099   | Fax: +966 12 676 4984 | www.alborglaboratories.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P
Sent: Tuesday, November 25, 2014 9:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue 
tek

Dear all
After processing in vip 6 the last paraffin has a strong odour of formalin

Have anybody experienced the same
Any ideas why it is happening.

Arun
Kuwait
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[Histonet] fixative

2014-11-25 Thread Schade, Adelle
Hello,
I am considering the following fixatives for a high school histology experiment 
and I would appreciate any input considering safety, disposal, etc.


1-   Ultrum II (American MasterTech):  promotes disposal in local sewer?

2-  Excell Plus (American MasterTech):  low-hazard but any idea on disposal 
from those who use it?

3-  Histochoice Tissue Fixative

Anyone using these products/ advice is greatly appreciated!

Ms. Adelle L. Schade, B.S., M.Ed.
Anatomy and Physiology
Conrad Weiser High School
44 Big Spring Rd.
Robesonia, PA  19551
610-693-8599 x6736
a_sch...@conradweiser.org

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Re: [Histonet] fixative

2014-11-25 Thread WILLIAM DESALVO
What are you going to accomplish? Alcohol will work as a fixative and 
dehydration solution. Less of a disposal issue.

William DeSalvo
william.desa...@sonoraquest.ccom
602-768-3692
Sent from my iPhone

 On Nov 25, 2014, at 8:30 AM, Schade, Adelle a_sch...@conradweiser.org wrote:
 
 Hello,
 I am considering the following fixatives for a high school histology 
 experiment and I would appreciate any input considering safety, disposal, etc.
 
 
 1-   Ultrum II (American MasterTech):  promotes disposal in local sewer?
 
 2-  Excell Plus (American MasterTech):  low-hazard but any idea on 
 disposal from those who use it?
 
 3-  Histochoice Tissue Fixative
 
 Anyone using these products/ advice is greatly appreciated!
 
 Ms. Adelle L. Schade, B.S., M.Ed.
 Anatomy and Physiology
 Conrad Weiser High School
 44 Big Spring Rd.
 Robesonia, PA  19551
 610-693-8599 x6736
 a_sch...@conradweiser.org
 
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Re: [Histonet] fixative

2014-11-25 Thread Rene J Buesa
I think the best you should do is obtaining the MSDS for those fixatives and 
check their composition and disposal.René J. 

 On Tuesday, November 25, 2014 10:30 AM, Schade, Adelle 
a_sch...@conradweiser.org wrote:
   

 Hello,
I am considering the following fixatives for a high school histology experiment 
and I would appreciate any input considering safety, disposal, etc.


1-      Ultrum II (American MasterTech):  promotes disposal in local sewer?

2-      Excell Plus (American MasterTech):  low-hazard but any idea on disposal 
from those who use it?

3-      Histochoice Tissue Fixative

Anyone using these products/ advice is greatly appreciated!

Ms. Adelle L. Schade, B.S., M.Ed.
Anatomy and Physiology
Conrad Weiser High School
44 Big Spring Rd.
Robesonia, PA  19551
610-693-8599 x6736
a_sch...@conradweiser.org

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RE: [EXTERNAL] Re: [Histonet] fixative

2014-11-25 Thread Roy, Ryan
I agree that alcohol is also less toxic. What type of tissue are you fixing?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Tuesday, November 25, 2014 10:46 AM
To: Schade, Adelle
Cc: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] fixative

What are you going to accomplish? Alcohol will work as a fixative and 
dehydration solution. Less of a disposal issue.

William DeSalvo
william.desa...@sonoraquest.ccom
602-768-3692
Sent from my iPhone

 On Nov 25, 2014, at 8:30 AM, Schade, Adelle a_sch...@conradweiser.org wrote:
 
 Hello,
 I am considering the following fixatives for a high school histology 
 experiment and I would appreciate any input considering safety, disposal, etc.
 
 
 1-   Ultrum II (American MasterTech):  promotes disposal in local sewer?
 
 2-  Excell Plus (American MasterTech):  low-hazard but any idea on 
 disposal from those who use it?
 
 3-  Histochoice Tissue Fixative
 
 Anyone using these products/ advice is greatly appreciated!
 
 Ms. Adelle L. Schade, B.S., M.Ed.
 Anatomy and Physiology
 Conrad Weiser High School
 44 Big Spring Rd.
 Robesonia, PA  19551
 610-693-8599 x6736
 a_sch...@conradweiser.org
 
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[Histonet] Kidney bx transport

2014-11-25 Thread Gagnon, Eric
We receive kidney biopsies brought directly to our grossing area within a few 
minutes after the cores are obtained. Previously, this was done by 
nephrologists, but since the renal biopsies are performed in radiology, the 
interventional radiologist brings them. We decide adequacy first, then 
apportion specimen to EM, IF and formalin.



Similar to the protocol Tim has mentioned, our cores are received on 
saline-soaked pads in a petri dish. We transfer them to a slide wetted with 
saline to assess under light microscopy.



It seems to come down to a decision of who wants to go where :) The IVR folks 
are willing to bring the biopsies to our laboratory - they get instant 
feedback, and often use the exchange for teaching purposes as we are a tertiary 
care academic centre. Another factor would be the location of microscope used 
for visualizing adequacy of the cores. The distance between the two locations 
is not huge, and there is always an MLT instantly available to assess the cores.



Rarely has this system had hiccups. Occasionally, the adequacy is a 
question-mark, and we err on the side of asking IVR to obtain more tissue. This 
is usually delivered within 5-10 minutes after we request it.



Hope this helps,



Eric Gagnon MLT

Histology Laboratory

Kingston General Hospital

Kingston, Ontario, Canada


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RE: [EXTERNAL] Re: [Histonet] fixative

2014-11-25 Thread Schade, Adelle

Some of our samples are from preserved specimen.  
We have a significant number of students that work/live on farms as well so 
from time to time we get a chick embryo that did not survive.
Also, we have researched and would like to try some plant/seed histology 
processes.

If alcohol will produce similar results without excessive dehydration that 
would be a great option for this setting.
Thanks for the input
Adelle

-Original Message-
From: Roy, Ryan [mailto:ryan@va.gov] 
Sent: Tuesday, November 25, 2014 11:49 AM
To: 'WILLIAM DESALVO'; Schade, Adelle
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [EXTERNAL] Re: [Histonet] fixative

I agree that alcohol is also less toxic. What type of tissue are you fixing?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Tuesday, November 25, 2014 10:46 AM
To: Schade, Adelle
Cc: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] fixative

What are you going to accomplish? Alcohol will work as a fixative and 
dehydration solution. Less of a disposal issue.

William DeSalvo
william.desa...@sonoraquest.ccom
602-768-3692
Sent from my iPhone

 On Nov 25, 2014, at 8:30 AM, Schade, Adelle a_sch...@conradweiser.org wrote:
 
 Hello,
 I am considering the following fixatives for a high school histology 
 experiment and I would appreciate any input considering safety, disposal, etc.
 
 
 1-   Ultrum II (American MasterTech):  promotes disposal in local sewer?
 
 2-  Excell Plus (American MasterTech):  low-hazard but any idea on 
 disposal from those who use it?
 
 3-  Histochoice Tissue Fixative
 
 Anyone using these products/ advice is greatly appreciated!
 
 Ms. Adelle L. Schade, B.S., M.Ed.
 Anatomy and Physiology
 Conrad Weiser High School
 44 Big Spring Rd.
 Robesonia, PA  19551
 610-693-8599 x6736
 a_sch...@conradweiser.org
 
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

2014-11-25 Thread Jennifer MacDonald
There may be a leaking valve. 



From:   Jamal j.rowa...@alborglaboratories.com
To: 'Arun Jyothi S.P' arunjyoth...@gmail.com, 
histonet@lists.utsouthwestern.edu
Date:   11/25/2014 12:21 AM
Subject:RE: [Histonet] Formalin smell in the last paraffin station 
in vip  6   tissue tek
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hi
If the paraffin are overheated it gives abnormal odor.
Recheck the paraffin bath temperature and the paraffin melting point then 
make sure to adjust the bath temperature at 2 degrees above the melting 
point.
Please update me.


Best Regards,


Jamal M. Al Rowaihi  Anatomic Pathology Supervisor   | Al 
Borg Medical Laboratories |  Mobile +966 503629832| 
j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| 
Phone: +966 12 670 0099| Fax: +966 12 676 4984 | 
www.alborglaboratories.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi 
S.P
Sent: Tuesday, November 25, 2014 9:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 
tissue tek

Dear all
After processing in vip 6 the last paraffin has a strong odour of formalin

Have anybody experienced the same
Any ideas why it is happening.

Arun
Kuwait
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RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

2014-11-25 Thread Cooper, Brian
Not sure if it's the same scenario, but this happened to us once about a dozen 
years ago on an older version of a VIP.  Luckily, I haven't seen this again!  

One of our lab assistants loaded a basket of formalin-fixed tissues onto a 
processor that hadn't yet been through the clean cycle.  The machine wouldn't 
let him start the process, so he took the basket out and put it back into 
formalin to wait while he ran the clean cycle.  For that brief moment that the 
basket was in the retort, a bunch of residual formalin drained off the tissues 
and basket and mixed in with the residual molten paraffin that was left in the 
bottom of the retort.  Anyway, after he ran the clean cycle he started the run 
as usual.  8 hours later, our blocks smelled like formalin when we cut them, 
and they were all mushy in texture.   

So Sakura told us that whenever you run the clean cycle, the VIP (again, it was 
a really old model and I'm not sure if the technology is still the same) will 
attempt to draw back into the last station that was used, any residual reagent 
that was left in the retort before proceeding to the next reagent.  Since the 
last reagent had been paraffin, anything in the retort (including the newly 
mixed in formalin) went back into the last paraffin chamber on the VIP.  As 
such, the next basket of tissues were infiltrated with formalin-infused 
paraffin in the very last processing step.   If I recall, we had to reprocess 
almost all of those blocks.  

Hope this helps.   

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P
Sent: Monday, November 24, 2014 10:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue 
tek

Dear all
After processing in vip 6 the last paraffin has a strong odour of formalin

Have anybody experienced the same
Any ideas why it is happening.

Arun
Kuwait
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[Histonet] RE: HER2 scoring exercises for breast cases

2014-11-25 Thread Joana Moreira
Hi!
I found the Leica Bond Oracle HER2 IHC e-Learning quite informative.
You can find it at the bottom of the following page: 
http://www.leicabiosystems.com/ihc-ish/novocastra-reagents/theranostics/details/product/leica-oracle-her2-bond-ihc-system-2/
And if you're interested the Leica BOND HER2 FISH e-Learning course is good too.
Joana


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[Histonet] RE: Kidney bx transport

2014-11-25 Thread Tony Henwood (SCHN)
Try a cell culture solution like Hanks.

It preserves better than normal saline (which despite its name is not isotonic 
with cells).
It will keep a renal and skin biopsy quite well for at least an hour.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric
Sent: Wednesday, 26 November 2014 3:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Kidney bx transport

We receive kidney biopsies brought directly to our grossing area within a few 
minutes after the cores are obtained. Previously, this was done by 
nephrologists, but since the renal biopsies are performed in radiology, the 
interventional radiologist brings them. We decide adequacy first, then 
apportion specimen to EM, IF and formalin.



Similar to the protocol Tim has mentioned, our cores are received on 
saline-soaked pads in a petri dish. We transfer them to a slide wetted with 
saline to assess under light microscopy.



It seems to come down to a decision of who wants to go where :) The IVR folks 
are willing to bring the biopsies to our laboratory - they get instant 
feedback, and often use the exchange for teaching purposes as we are a tertiary 
care academic centre. Another factor would be the location of microscope used 
for visualizing adequacy of the cores. The distance between the two locations 
is not huge, and there is always an MLT instantly available to assess the cores.



Rarely has this system had hiccups. Occasionally, the adequacy is a 
question-mark, and we err on the side of asking IVR to obtain more tissue. This 
is usually delivered within 5-10 minutes after we request it.



Hope this helps,



Eric Gagnon MLT

Histology Laboratory

Kingston General Hospital

Kingston, Ontario, Canada


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RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

2014-11-25 Thread Tony Henwood (SCHN)
Also check the last alcohol and xylene.
Has someone accidentally replaced these solution with formalin by mistake?

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Wednesday, 26 November 2014 5:17 AM
To: Arun Jyothi S.P; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Formalin smell in the last paraffin station in vip 6 
tissue tek

Not sure if it's the same scenario, but this happened to us once about a dozen 
years ago on an older version of a VIP.  Luckily, I haven't seen this again!  

One of our lab assistants loaded a basket of formalin-fixed tissues onto a 
processor that hadn't yet been through the clean cycle.  The machine wouldn't 
let him start the process, so he took the basket out and put it back into 
formalin to wait while he ran the clean cycle.  For that brief moment that the 
basket was in the retort, a bunch of residual formalin drained off the tissues 
and basket and mixed in with the residual molten paraffin that was left in the 
bottom of the retort.  Anyway, after he ran the clean cycle he started the run 
as usual.  8 hours later, our blocks smelled like formalin when we cut them, 
and they were all mushy in texture.   

So Sakura told us that whenever you run the clean cycle, the VIP (again, it was 
a really old model and I'm not sure if the technology is still the same) will 
attempt to draw back into the last station that was used, any residual reagent 
that was left in the retort before proceeding to the next reagent.  Since the 
last reagent had been paraffin, anything in the retort (including the newly 
mixed in formalin) went back into the last paraffin chamber on the VIP.  As 
such, the next basket of tissues were infiltrated with formalin-infused 
paraffin in the very last processing step.   If I recall, we had to reprocess 
almost all of those blocks.  

Hope this helps.   

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P
Sent: Monday, November 24, 2014 10:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue 
tek

Dear all
After processing in vip 6 the last paraffin has a strong odour of formalin

Have anybody experienced the same
Any ideas why it is happening.

Arun
Kuwait
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RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

2014-11-25 Thread Arun Jyothi S.P
Dear all
We find the problem
The retort was over heating and thats y the condensation also very high
When the condensation chamber is full its pumping back the reagent in the
condensation chamber to the retort and mixing it with paraffin
Thanks for all the support
On 26 Nov 2014 02:39, Tony Henwood (SCHN) tony.henw...@health.nsw.gov.au
wrote:

 Also check the last alcohol and xylene.
 Has someone accidentally replaced these solution with formalin by mistake?

 Regards
 Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
 Laboratory Manager  Senior Scientist, the Children’s Hospital at Westmead
 Adjunct Fellow, School of Medicine, University of Western Sydney
 Tel: 612 9845 3306
 Fax: 612 9845 3318
 Pathology Department
 the children's hospital at westmead
 Cnr Hawkesbury Road and Hainsworth Street, Westmead
 Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
 Sent: Wednesday, 26 November 2014 5:17 AM
 To: Arun Jyothi S.P; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Formalin smell in the last paraffin station in vip
 6 tissue tek

 Not sure if it's the same scenario, but this happened to us once about a
 dozen years ago on an older version of a VIP.  Luckily, I haven't seen this
 again!

 One of our lab assistants loaded a basket of formalin-fixed tissues onto a
 processor that hadn't yet been through the clean cycle.  The machine
 wouldn't let him start the process, so he took the basket out and put it
 back into formalin to wait while he ran the clean cycle.  For that brief
 moment that the basket was in the retort, a bunch of residual formalin
 drained off the tissues and basket and mixed in with the residual molten
 paraffin that was left in the bottom of the retort.  Anyway, after he ran
 the clean cycle he started the run as usual.  8 hours later, our blocks
 smelled like formalin when we cut them, and they were all mushy in
 texture.

 So Sakura told us that whenever you run the clean cycle, the VIP (again,
 it was a really old model and I'm not sure if the technology is still the
 same) will attempt to draw back into the last station that was used, any
 residual reagent that was left in the retort before proceeding to the next
 reagent.  Since the last reagent had been paraffin, anything in the retort
 (including the newly mixed in formalin) went back into the last paraffin
 chamber on the VIP.  As such, the next basket of tissues were infiltrated
 with formalin-infused paraffin in the very last processing step.   If I
 recall, we had to reprocess almost all of those blocks.

 Hope this helps.

 Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of
 Pathology and Laboratory Medicine Children's Hospital Los Angeles
 bcoo...@chla.usc.edu

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P
 Sent: Monday, November 24, 2014 10:53 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Formalin smell in the last paraffin station in vip 6
 tissue tek

 Dear all
 After processing in vip 6 the last paraffin has a strong odour of formalin

 Have anybody experienced the same
 Any ideas why it is happening.

 Arun
 Kuwait
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 or legally privileged information. Any unauthorized review, use, disclosure
 or distribution is prohibited. If you are not the intended recipient,
 please contact the sender by reply e-mail and destroy all copies of this
 original message.

 -



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 If you are not the intended recipient, please delete it and notify the
 sender.

 Views expressed in this message and any attachments are those of the
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 Children's Hospitals Network.

 This note also confirms that this email message has been virus scanned and
 although no computer viruses were detected, The Sydney Childrens Hospital's
 Network accepts no liability for any consequential damage resulting from
 email containing computer viruses.

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