Is there any information available that would indicate the effect of
decolorizing an HE stained slide on subsequent IHC staining / antigenicity?
Thanks, Laurie
Laurie Best,BS
Histology and Cyto Prep Supervisor
Sunrise Medical Laboratories
250 Miller Place
Hicksville, N.Y. 11801
tel
Hi Bernice,
We have the TMArrayer from Pathology Devices and we love it.
Easy to use, we had no problem with it since 3 years and built quite a lot
of TMAs.
Véronique
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Histonet mailing list
Histonet@lists.utsouthwestern.edu
You can decolorize an HE stain and use the decolorized section for IHC
procedure. René J.
On Tuesday, December 2, 2014 6:36 AM, Laurie Best lb...@sunriselab.com
wrote:
Is there any information available that would indicate the effect of
decolorizing an HE stained slide on
Hello all,
I am searching for a weighing scale that will record multiple organ weights
during autopsy and then allow display of all data at the end of the session.
Ideally this would be a stand-alone unit rather than computer linked. Does
anyone have experience with this sort of equipment?
We also have the Grand Master. It's quite amazing, however we have had issues
with the punches occasionally. Also alignment issues which turned out to be
software related. Have you seen any of this Ashley??
Regards,
Angie
Sent from my iPhone
On Dec 2, 2014, at 8:59 AM, Troutman, Kenneth A
I beg to differThe VIP 5 is capable of processing 300 cassettes. On
the first page of my VIP 5, Operating Manual (p. 1.1), it says it is
capable of handling 150-300 cassettes.
Tina Van Meter
Histology Core Manager
Scripps Research Institute
Jupiter, FL
On Tue, Dec 2, 2014 at 3:22 AM, Jamal
Does anyone take daily temperature checks of their water bath (used for cutting
only) for CAP?
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph 610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net
This email
Speaking for myself and the labs I have managed, I have been checking ALL temps
and recording them since the early 90’s. These include waters baths for tissue
flotation, and ones used for special stains, ovens, refrigerators, freezers,
cryostats, tissue processors, embedding centers,
I just need a moment to ventis it just me or is everyone seeing more and
more back order issues with their various vendors? It is not specific to any
one particular vendor, product or even category of product, but lately I am
being told more and more often that an item is not
Yes, I have always done that at any lab I worked at.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this transmission may contain privileged and
confidential information,
Martha,
Yes, as a matter of fact I have been noticing this too! A real pain.
Jo Mauger
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha
Ward-Pathology
Sent: Tuesday, December 02, 2014 10:48 AM
To:
Is your lab in need of a new microscope or replacement power supply?
We have several Olympus BX40 and BX41 compound microscopes. Also, we offer
a solution to the broken rheostat (light switch) problem that is common
with the BX40.
Please Email or call with questions regarding Olympus Microscope
Yes, I am having this issue with one vendor in particular (which I won't
mention). It has affected our operations and it is very frustrating!
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The
Happens every year at this time with various vendors and end of year. I always
over order in October to be sure I am good through the end of the year or as
close as I can. I know the things I use most and those are just what is always
in backorder at the end of the year. October became my
It is no longer a CAP requirement to do this, so we haven't for many years.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is
At least I know I am not crazy or being paranoid! :) this year just seems
to be worse than I ever remember it beingand the worse part is while I am
freaking out because I can't get something I desperately need, the customer
service person can only say We are sorry for the
Hello,
I agree, we record daily all our temperatures for all equipment in histology.
Ryan Roy HTL (ASCP)cm
Veterans Affairs Medical Center
Manchester NH
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
We still do, due to the fact that it is on our general temperature chart that
is used in the department.
Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
In the All Common checklist, COM.30750, a Phase II, addresses this and it is a
requirement.
Temperatures are checked and recorded each day of use for all
temperature-dependent equipment and environments using a calibrated
thermometer.
-Original Message-
From:
Thank you to everyone who has responded. They have a more specific question:
Currently they are using SurgiPath DeCal solution II. They are being asked
to use an EDTA decal solution that will provide the best preservation of RNA
and DNA proteins. What is everyone using for this
We record daily temperatures of water baths used for cutting.
Patti L. McDavid
Laboratory Manager
4500 Thirteenth Street
P.O. Box 1810
Gulfport, MS 39502-1810
Phone 228-575-2340
Fax 228-865-3325
pmcda...@mhg.commailto:pmcda...@mhg.com
We have used VIP in the past and it was adequate. We use the
Leica Peloris as our main processor and love it.
Patti L. McDavid
Laboratory Manager
4500 Thirteenth Street
P.O. Box 1810
Gulfport, MS 39502-1810
Phone 228-575-2340
Fax 228-865-3325
pmcda...@mhg.commailto:pmcda...@mhg.com
Same thing here.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patti McDavid
Sent: Tuesday, December 02, 2014 11:22 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Tissue Processor
We
Laurie,
The slides must be pre-coated with poly-L-lisine or any other type adhesive,
suitable for IHC.
IHC is done usually for a several markers. Do you have a several HE slides?
Sincerely,
Maxim Peshkov
Russia,
Taganrog. mailto:maxim...@mail.ru
Surgical Secretary Compentancy - Funny you should mention this as I had
just finished a review containing just such a competency, just before
reading this.
Our Surgical Secretaries take an annual typing test for word speed, have
to demonstrate knowledge of certain computer functions, proficiency
Hello all,
I've done some research on Foxp3 and Geminin and I've seen a few antibodies
that look sufficient for IHC in FFPE tissues. My lab is currently looking into
purchasing these antibodies for a few different projects.
Does anyone on here use these two antibodies for IHC in FFPE tissues?
I suggest you run two positive controls. One control stained with HE the
decolorized. The other as usual.
JB
On Dec 2, 2014 3:35 AM, Laurie Best lb...@sunriselab.com wrote:
Is there any information available that would indicate the effect of
decolorizing an HE stained slide on subsequent IHC
Does anyone know if you have to use controls for IHC on MOHS frozen section
procedures to satisfy CAP requirements?
Thank you,
Cherie Chapman, BS, HT, HTL (ASCP)
Associate Director of Dermatopathology Laboratory
University of Missouri Department of Dermatology
University Physicians Medical
I would think you would have to validate IHC for MOHS the same as FFPE, once
the validation process is done maybe controls would not be necessary.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
To be honest we take every temp but that. Really do not see the need
Stp
TJUH
Sent from my Verizon Wireless 4G LTE smartphone
Original message
From: Abbott, Tanya tanyaabb...@catholichealth.net
Date:12/02/2014 10:29 AM (GMT-05:00)
To: histonet@lists.utsouthwestern.edu
We have been checking ALL temps and recording every shift. These include
cryostats, Embedding centers, Wax dispenser, Hot plates, Hot Ovens, Body
Refrigerator, refrigerators, Water Baths, and room temps and Humidity%.
Muhammad Tahseen MLT (PMF), MT (JIMTEF) Japan
Senior supervisor
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