[Histonet] Decolorizing effect on IHC stain
Is there any information available that would indicate the effect of decolorizing an HE stained slide on subsequent IHC staining / antigenicity? Thanks, Laurie Laurie Best,BS Histology and Cyto Prep Supervisor Sunrise Medical Laboratories 250 Miller Place Hicksville, N.Y. 11801 tel (631)435-1515x1018 fax(631)435-2014 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: TMA arrayer
Hi Bernice, We have the TMArrayer from Pathology Devices and we love it. Easy to use, we had no problem with it since 3 years and built quite a lot of TMAs. Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Decolorizing effect on IHC stain
You can decolorize an HE stain and use the decolorized section for IHC procedure. René J. On Tuesday, December 2, 2014 6:36 AM, Laurie Best lb...@sunriselab.com wrote: Is there any information available that would indicate the effect of decolorizing an HE stained slide on subsequent IHC staining / antigenicity? Thanks, Laurie Laurie Best,BS Histology and Cyto Prep Supervisor Sunrise Medical Laboratories 250 Miller Place Hicksville, N.Y. 11801 tel (631)435-1515x1018 fax(631)435-2014 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mortuary scales advice
Hello all, I am searching for a weighing scale that will record multiple organ weights during autopsy and then allow display of all data at the end of the session. Ideally this would be a stand-alone unit rather than computer linked. Does anyone have experience with this sort of equipment? Thank you Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.fin...@glasgow.ac.ukmailto:finlay.fin...@glasgow.ac.uk The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] TMA arrayer
We also have the Grand Master. It's quite amazing, however we have had issues with the punches occasionally. Also alignment issues which turned out to be software related. Have you seen any of this Ashley?? Regards, Angie Sent from my iPhone On Dec 2, 2014, at 8:59 AM, Troutman, Kenneth A ashley.trout...@vanderbilt.edu wrote: Hello Bernice, We have a TMA Grand Master from Perkin Elmer. It is quite a machine. Let me know by email if you would like contact information. Regards, Ashley Troutman BS, MBA, HT(ASCP)QIHC Supervisor-Translational Pathology Shared Resource Vanderbilt University Medical Center S-1310 Medical Center North 1161 21st Avenue South Nashville, TN 37232 Message: 1 Date: Mon, 1 Dec 2014 14:34:23 + From: Bernice Frederick b-freder...@northwestern.edumailto:b-freder...@northwestern.edu Subject: [Histonet] TMA arrayer To: Histonet (histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Message-ID: 62c639732d3f274daced033ebdf6adaf2f131...@evcspmbx2.ads.northwestern.edumailto:62c639732d3f274daced033ebdf6adaf2f131...@evcspmbx2.ads.northwestern.edu Content-Type: text/plain; charset=us-ascii Hello all, If anyone has a semi-automated arrayer, where did you get it? We have one from Veridiam, but are wondering if anyone else makes them. We are looking to purchase another unit. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edumailto:b-freder...@northwestern.edu%3cmailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processor
I beg to differThe VIP 5 is capable of processing 300 cassettes. On the first page of my VIP 5, Operating Manual (p. 1.1), it says it is capable of handling 150-300 cassettes. Tina Van Meter Histology Core Manager Scripps Research Institute Jupiter, FL On Tue, Dec 2, 2014 at 3:22 AM, Jamal j.rowa...@alborglaboratories.com wrote: Hi Sakura VIP 5 dose not process more than 150 cassettes per day I recommend Sakura VIP 6 or Leica ASP 6025 Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tina Van Meter Sent: Monday, December 01, 2014 10:15 PM To: Abbott, Tanya Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Processor Sakura VIP 5 or 6 On Mon, Dec 1, 2014 at 2:03 PM, Abbott, Tanya tanyaabb...@catholichealth.net wrote: Looking for a tissue processor to process 200-300 cassettes per run, any favourites? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Water baths
Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Water baths
Speaking for myself and the labs I have managed, I have been checking ALL temps and recording them since the early 90’s. These include waters baths for tissue flotation, and ones used for special stains, ovens, refrigerators, freezers, cryostats, tissue processors, embedding centers, decloaking chambers and room temps. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com Tele: (831) 375-3577 X117 On Dec 2, 2014, at 7:29 AM, Abbott, Tanya tanyaabb...@catholichealth.net wrote: Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] back orders from various vendors
I just need a moment to ventis it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not availableexpected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems . Thanks for letting get this off my chest.I will resume my frantic search for another antibody vendor now. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Water baths
Yes, I have always done that at any lab I worked at. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 7:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: back orders from various vendors
Martha, Yes, as a matter of fact I have been noticing this too! A real pain. Jo Mauger -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, December 02, 2014 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back orders from various vendors I just need a moment to ventis it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not availableexpected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems . Thanks for letting get this off my chest.I will resume my frantic search for another antibody vendor now. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microscope Problems?
Is your lab in need of a new microscope or replacement power supply? We have several Olympus BX40 and BX41 compound microscopes. Also, we offer a solution to the broken rheostat (light switch) problem that is common with the BX40. Please Email or call with questions regarding Olympus Microscope issues. -- Scott Munday Phone: 919-775-5596 Fax: 919-776-9566 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: back orders from various vendors
Yes, I am having this issue with one vendor in particular (which I won't mention). It has affected our operations and it is very frustrating! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, December 02, 2014 7:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back orders from various vendors I just need a moment to ventis it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not availableexpected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems . Thanks for letting get this off my chest.I will resume my frantic search for another antibody vendor now. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: back orders from various vendors
Happens every year at this time with various vendors and end of year. I always over order in October to be sure I am good through the end of the year or as close as I can. I know the things I use most and those are just what is always in backorder at the end of the year. October became my answer as long as expiration dates are not an issue. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, December 02, 2014 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back orders from various vendors I just need a moment to ventis it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not availableexpected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems . Thanks for letting get this off my chest.I will resume my frantic search for another antibody vendor now. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Water baths
It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: back orders from various vendors
At least I know I am not crazy or being paranoid! :) this year just seems to be worse than I ever remember it beingand the worse part is while I am freaking out because I can't get something I desperately need, the customer service person can only say We are sorry for the inconvenience. Argg -Original Message- From: Marcum, Pamela A [mailto:pamar...@uams.edu] Sent: Tuesday, December 02, 2014 10:59 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: back orders from various vendors Happens every year at this time with various vendors and end of year. I always over order in October to be sure I am good through the end of the year or as close as I can. I know the things I use most and those are just what is always in backorder at the end of the year. October became my answer as long as expiration dates are not an issue. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, December 02, 2014 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back orders from various vendors I just need a moment to ventis it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not availableexpected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems . Thanks for letting get this off my chest.I will resume my frantic search for another antibody vendor now. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [EXTERNAL] Re: [Histonet] Water baths
Hello, I agree, we record daily all our temperatures for all equipment in histology. Ryan Roy HTL (ASCP)cm Veterans Affairs Medical Center Manchester NH -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Tuesday, December 02, 2014 10:47 AM To: Abbott, Tanya Cc: Histonet Subject: [EXTERNAL] Re: [Histonet] Water baths Speaking for myself and the labs I have managed, I have been checking ALL temps and recording them since the early 90’s. These include waters baths for tissue flotation, and ones used for special stains, ovens, refrigerators, freezers, cryostats, tissue processors, embedding centers, decloaking chambers and room temps. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.commailto:aalli...@montereygi.com Tele: (831) 375-3577 X117 On Dec 2, 2014, at 7:29 AM, Abbott, Tanya tanyaabb...@catholichealth.netmailto:tanyaabb...@catholichealth.net wrote: Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.netmailto:tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Water baths
We still do, due to the fact that it is on our general temperature chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com www.parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Water baths
In the All Common checklist, COM.30750, a Phase II, addresses this and it is a requirement. Temperatures are checked and recorded each day of use for all temperature-dependent equipment and environments using a calibrated thermometer. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, December 02, 2014 11:50 AM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths We still do, due to the fact that it is on our general temperature chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com www.parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: EDTA decalcification solution follow up questions
Thank you to everyone who has responded. They have a more specific question: Currently they are using SurgiPath DeCal solution II. They are being asked to use an EDTA decal solution that will provide the best preservation of RNA and DNA proteins. What is everyone using for this purpose? Thanks again for any information Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Monday, December 01, 2014 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EDTA decalcification solution I am asking this question for our Histology Lab. They are being asked about using EDTA as a decal solution for bone and wondered if anyone else is using this?Is this available as a ready to use? Or do you have to make it up? What vendors are you using and could you provide a procedure about how you use itlength of time in solution, etc. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Water Baths
We record daily temperatures of water baths used for cutting. Patti L. McDavid Laboratory Manager 4500 Thirteenth Street P.O. Box 1810 Gulfport, MS 39502-1810 Phone 228-575-2340 Fax 228-865-3325 pmcda...@mhg.commailto:pmcda...@mhg.com [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. § 75-24-29), the Privacy Act of 1974 (5 U.S.C. § 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **PRIVATE AND CONFIDENTIAL** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor
We have used VIP in the past and it was adequate. We use the Leica Peloris as our main processor and love it. Patti L. McDavid Laboratory Manager 4500 Thirteenth Street P.O. Box 1810 Gulfport, MS 39502-1810 Phone 228-575-2340 Fax 228-865-3325 pmcda...@mhg.commailto:pmcda...@mhg.com [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. § 75-24-29), the Privacy Act of 1974 (5 U.S.C. § 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **PRIVATE AND CONFIDENTIAL** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Tissue Processor
Same thing here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patti McDavid Sent: Tuesday, December 02, 2014 11:22 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Processor We have used VIP in the past and it was adequate. We use the Leica Peloris as our main processor and love it. Patti L. McDavid Laboratory Manager 4500 Thirteenth Street P.O. Box 1810 Gulfport, MS 39502-1810 Phone 228-575-2340 Fax 228-865-3325 pmcda...@mhg.commailto:pmcda...@mhg.com [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. § 75-24-29), the Privacy Act of 1974 (5 U.S.C. § 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **PRIVATE AND CONFIDENTIAL** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Decolorizing effect on IHC stain
Laurie, The slides must be pre-coated with poly-L-lisine or any other type adhesive, suitable for IHC. IHC is done usually for a several markers. Do you have a several HE slides? Sincerely, Maxim Peshkov Russia, Taganrog. mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Surgical Secretary Compentancy
Surgical Secretary Compentancy - Funny you should mention this as I had just finished a review containing just such a competency, just before reading this. Our Surgical Secretaries take an annual typing test for word speed, have to demonstrate knowledge of certain computer functions, proficiency at using certain LIS reporting functions, meeting typing and send out TAT and also a few basic office items, such as answering the phone correctly and billing of miscellaneous send out tests. I hope this helps, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -Original Message- Sent: Tuesday, December 02, 2014 9:51 AM Subject: Histonet Digest, Vol 133, Issue 2 Today's Topics: 1. Competency Assessment (Abbott, Tanya) Does any have their Surgical Secretaries perform any sort of annual competency? If so, any ideas you could share please? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FoxP3 and Geminin Immunohistochemistry
Hello all, I've done some research on Foxp3 and Geminin and I've seen a few antibodies that look sufficient for IHC in FFPE tissues. My lab is currently looking into purchasing these antibodies for a few different projects. Does anyone on here use these two antibodies for IHC in FFPE tissues? I'm going to use them in bright field, but we may also turn to IF for other studies. I'd be grateful for any information from what clone/company do you buy from and retrieval methods to primary antibody incubations/dilutions. Thank you all for any information, Steven Swartwood HT(ASCP) Cedars Sinai Medical Center steven.swartw...@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Decolorizing effect on IHC stain
I suggest you run two positive controls. One control stained with HE the decolorized. The other as usual. JB On Dec 2, 2014 3:35 AM, Laurie Best lb...@sunriselab.com wrote: Is there any information available that would indicate the effect of decolorizing an HE stained slide on subsequent IHC staining / antigenicity? Thanks, Laurie Laurie Best,BS Histology and Cyto Prep Supervisor Sunrise Medical Laboratories 250 Miller Place Hicksville, N.Y. 11801 tel (631)435-1515x1018 fax(631)435-2014 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Does anyone know if you have to use controls for IHC on MOHS frozen section procedures to satisfy CAP requirements? Thank you, Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
I would think you would have to validate IHC for MOHS the same as FFPE, once the validation process is done maybe controls would not be necessary. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net From: chapm...@health.missouri.edu To: histonet@lists.utsouthwestern.edu Date: Tue, 2 Dec 2014 21:22:38 + Subject: [Histonet] (no subject) Does anyone know if you have to use controls for IHC on MOHS frozen section procedures to satisfy CAP requirements? Thank you, Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water baths
To be honest we take every temp but that. Really do not see the need Stp TJUH Sent from my Verizon Wireless 4G LTE smartphone Original message From: Abbott, Tanya tanyaabb...@catholichealth.net Date:12/02/2014 10:29 AM (GMT-05:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Water baths
We have been checking ALL temps and recording every shift. These include cryostats, Embedding centers, Wax dispenser, Hot plates, Hot Ovens, Body Refrigerator, refrigerators, Water Baths, and room temps and Humidity%. Muhammad Tahseen MLT (PMF), MT (JIMTEF) Japan Senior supervisor histopathology SKMCHRC Lahore Pakistan -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 8:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
