RE: [Histonet] Re: Embedding
We just use an empty lid top to the slide boxes. We save them as we load the slide printer, to be used for this and many other tasks. Kind Regards! John J Shelley Research Specialist, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Lab: (407) 745-2119 Fax: (407) 745-2001 email: jshel...@sanfordburnham.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, January 22, 2015 1:14 PM To: gayle.cal...@bresnan.net; 'Tony Auge'; histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding We use a urine specimen container under the right lower corner of the para trimmer and toss it each day. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Gayle Callis [gayle.cal...@bresnan.net] Sent: Thursday, January 22, 2015 1:03 PM To: 'Tony Auge'; histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding A fabulous idea! I suspect one could use a cheap travel iron although one needs to devise a way to collect melted paraffin. Even our fancy para trimmer didn't have catch pan for paraffin drippings.I suggest using a new or receycled aluminum baking pans available in supermarkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Thursday, January 22, 2015 9:58 AM To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa Subject: Re: [Histonet] Re: Embedding If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Embedding
We use empty pipette tip boxes. They are the perfect size and fit right under the edge and we just toss them when them get full. Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, January 22, 2015 10:14 AM To: gayle.cal...@bresnan.net; 'Tony Auge'; histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding We use a urine specimen container under the right lower corner of the para trimmer and toss it each day. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Gayle Callis [gayle.cal...@bresnan.net] Sent: Thursday, January 22, 2015 1:03 PM To: 'Tony Auge'; histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding A fabulous idea! I suspect one could use a cheap travel iron although one needs to devise a way to collect melted paraffin. Even our fancy para trimmer didn't have catch pan for paraffin drippings.I suggest using a new or receycled aluminum baking pans available in supermarkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Thursday, January 22, 2015 9:58 AM To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa Subject: Re: [Histonet] Re: Embedding If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] billing question
That's correct, Hazel. Daniel Schneider, MD Amarillo, TX On Thu, Jan 22, 2015 at 3:14 PM, Horn, Hazel V hor...@archildrens.org wrote: Scenario: I am billing for immunostains and there are 3 specimens; A, B, C. Pathologist orders 1 stain on all 3 specimens. I bill 3 88342's. Pathologist orders 2 stains each on A and B. I bill 2 88342's and 2 88341's. Is this correct? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.orgmailto:hor...@archildrens.org archildrens.orghttp://www.archildrens.org/ ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryosectioning biopsies
Hello-In order to preserve scant tissue for future molecular testing, is anyone processing their (needle) biopsies using only frozen sections and subsequently storing the frozen specimens...and NOT paraffin processing them AT ALL? Kate Kathleen Caleri BS HT(ASCP) Histology Lab Supervisor Roswell Park Cancer Institute Elm and Carlton Streets Buffalo, NY 14263 716-845-1329 There is pleasure in the pathless woods, there is rapture on the lonely shore, There is society where none intrude, by the deep sea and in its roar: I love not Man the less...but Nature more. Lord Byron This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tech Time:
I am in need of metrics for the use of requesting an additional histotech. Does anyone have a system in place for presenting the need for additional FTE based on volume, workflow, that you can share? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Embedding
Wow! Now I really feel lucky. My cheap para trimmer came with a wax catcher with disposable liners that is magnetically attached to the trimmer. http://www.tedpella.com/histo_html/histo1.htm.aspx#_28154 Cant't beat $40 and a practical mind-set though. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, January 22, 2015 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Embedding We use empty pipette tip boxes. They are the perfect size and fit right under the edge and we just toss them when them get full. Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, January 22, 2015 10:14 AM To: gayle.cal...@bresnan.net; 'Tony Auge'; histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding We use a urine specimen container under the right lower corner of the para trimmer and toss it each day. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Gayle Callis [gayle.cal...@bresnan.net] Sent: Thursday, January 22, 2015 1:03 PM To: 'Tony Auge'; histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding A fabulous idea! I suspect one could use a cheap travel iron although one needs to devise a way to collect melted paraffin. Even our fancy para trimmer didn't have catch pan for paraffin drippings.I suggest using a new or receycled aluminum baking pans available in supermarkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Thursday, January 22, 2015 9:58 AM To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa Subject: Re: [Histonet] Re: Embedding If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Embedding
That is so cool!! I wish I had one of those!! By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Wed, Jan 21, 2015 at 1:59 PM, histot...@imagesbyhopper.com histot...@imagesbyhopper.com wrote: We also use the para-trimmer. In my view, it is worth its weight in gold! I can melt 5 blocks at a time, works like a charm. I am one who does not mind the wax on the sides, as I am most confident that there is enough paraffin to support the cassette. Michelle Sent from my iPhone On Jan 21, 2015, at 11:41 AM, Morken, Timothy timothy.mor...@ucsf.edu wrote: I agree. We got one of these a couple years ago and the techs love it. It is a heated block on which you rub the cassette. The paraffin melts away. It is especially good for preserving barcodes (but don't press the printed surface on the heat block too long - you can soften the print and cause some damage, but nothing like can happen with scraping). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, January 21, 2015 8:29 AM To: gayle.cal...@bresnan.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Embedding I agree with Gayle. We finally purchased a trimmer from Ted Pella - lowest price by far - and are saving our finger joints. The amount of wax remaining on the cassette also appears to depend on the brand of mold used. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Wednesday, January 21, 2015 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Embedding After years of never winning the battle of paraffin on cassette edges after embedding, we purchased a paraffin block trimmer. It saves time and the stress on finger joints compared to scraping cassettes daily. No matter how careful we were during embedding to keep excess paraffin off cassette edges, we were never successful. Several vendors have these and you may be able to find a refurbished one. Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [EXTERNAL] Re: [Histonet] Re: Embedding
I use a block trimmer. I think it is safer for wrists. Being that you push the block against it and I seemed to always pull when I scraped blocks. I am guitar player and need my wrists. I don't think it is a strong argument to say its faster then hand scraping atleast not until a good technique is developed by the tech. Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opions of the VA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, January 22, 2015 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Re: Embedding That is so cool!! I wish I had one of those!! By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Wed, Jan 21, 2015 at 1:59 PM, histot...@imagesbyhopper.com histot...@imagesbyhopper.com wrote: We also use the para-trimmer. In my view, it is worth its weight in gold! I can melt 5 blocks at a time, works like a charm. I am one who does not mind the wax on the sides, as I am most confident that there is enough paraffin to support the cassette. Michelle Sent from my iPhone On Jan 21, 2015, at 11:41 AM, Morken, Timothy timothy.mor...@ucsf.edu wrote: I agree. We got one of these a couple years ago and the techs love it. It is a heated block on which you rub the cassette. The paraffin melts away. It is especially good for preserving barcodes (but don't press the printed surface on the heat block too long - you can soften the print and cause some damage, but nothing like can happen with scraping). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, January 21, 2015 8:29 AM To: gayle.cal...@bresnan.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Embedding I agree with Gayle. We finally purchased a trimmer from Ted Pella - lowest price by far - and are saving our finger joints. The amount of wax remaining on the cassette also appears to depend on the brand of mold used. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Wednesday, January 21, 2015 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Embedding After years of never winning the battle of paraffin on cassette edges after embedding, we purchased a paraffin block trimmer. It saves time and the stress on finger joints compared to scraping cassettes daily. No matter how careful we were during embedding to keep excess paraffin off cassette edges, we were never successful. Several vendors have these and you may be able to find a refurbished one. Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Morgue
From: Carol Fields Sent: Thursday, January 22, 2015 11:17 AM To: 'histonet-boun...@lists.utsouthwestern.edu' Subject: Morgue Hi All, Could someone tell me what needs to be posted in the Morgue for inspection please. I know CJD info., this is a new morgue and I'm not sure what all is needed. Thank you in advance. Carole Carole Fields,, HT (ASCP) Lead Histotechnologist, Pathology Laboratory Martin Luther King Jr. Community Hospital 1680 E. 120th Street Los Angeles, CA 90059 O:424-338-8000 x 8306 cfie...@mlkch.orgmailto:cfie...@mlkch.org www.MLKCommunityHospital.orghttp://www.mlkcommunityhospital.org/ www.facebook.com/YourMLKCHhttp://www.facebook.com/YourMLKCH www.twitter.com/YourMLKCHhttp://www.twitter.com/YourMLKCH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Embedding
If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Embedding
A fabulous idea! I suspect one could use a cheap travel iron although one needs to devise a way to collect melted paraffin. Even our fancy para trimmer didn't have catch pan for paraffin drippings.I suggest using a new or receycled aluminum baking pans available in supermarkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Thursday, January 22, 2015 9:58 AM To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa Subject: Re: [Histonet] Re: Embedding If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Embedding
We use a urine specimen container under the right lower corner of the para trimmer and toss it each day. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Gayle Callis [gayle.cal...@bresnan.net] Sent: Thursday, January 22, 2015 1:03 PM To: 'Tony Auge'; histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding A fabulous idea! I suspect one could use a cheap travel iron although one needs to devise a way to collect melted paraffin. Even our fancy para trimmer didn't have catch pan for paraffin drippings.I suggest using a new or receycled aluminum baking pans available in supermarkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Thursday, January 22, 2015 9:58 AM To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa Subject: Re: [Histonet] Re: Embedding If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Marker for embedding material?
Hi Histonetters, I'm working on embedding bacterial cells in Technovit 8100 and am curious if folks have any recommendations for dyes that can be used as a marker. I first embed my cells in a small agarose plug, then proceed to infiltrate and embed in Technovit 8100. Its possible to see the little agarose plug and flecks of cell material but can be tricky! Does anyone have suggestions for a good dye or marker that I could mix into the agarose so I can better see when trimming and sectioning the block? The only caveat is that it must not be autofluorescent or interfere with *in situ* hybridization we do downstream on these sections. Thanks in advance! Lizzy -- The obvious goal of any bacterium is to become bacteria. Lizzy Wilbanks Agouron Postdoctoral Fellow, Orphan Lab Division of Geological and Planetary Sciences California Institute of Technology, MC 100-23 Pasadena, CA 91125 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Her2 IHC validation
In advance of preparing for our CAP inspection window, I am working on our validation write-up for our Her2 IHC and was looking for some data concerning the correlation rates.We compared our IHC staining to FISH Her2 results on the same case. Is there a minimum correlation rate? I have been unable to find one in my reading. Thank you in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Her2 IHC validation
Yes, check the ASCO/CAP guidelines there are correlation rates for positives and negatives. Joelle Weaver MAOM, HTL (ASCP) QIHC From: mw...@wakehealth.edu To: histonet@lists.utsouthwestern.edu Date: Thu, 22 Jan 2015 20:21:53 + Subject: [Histonet] Her2 IHC validation In advance of preparing for our CAP inspection window, I am working on our validation write-up for our Her2 IHC and was looking for some data concerning the correlation rates.We compared our IHC staining to FISH Her2 results on the same case. Is there a minimum correlation rate? I have been unable to find one in my reading. Thank you in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cheap Disposable Blades for Facing In
Hello everyone, Does anyone have a source for cheap, low-profile blades for facing in blocks? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology Necropsy Supervisor, President Keith Richards Fan Club UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
