[Histonet] New lab setup
I am working on setting up an adequate validation study for tissue processing at a new lab. I want it to be adequate but also not too labor intensive or costly. I have been in Histotechnology for over 36 years and have done this before in the lab I can from. Unfortunately sometimes I make things more complicated than others. I realize the standards for testing new protocols and antibodies are defined in the CAP checklist but routine tissue processing just says to document the procedure and to run parallel samples as a blind study. We are going to run only GI biopsies at first and I had planned on running parallel samples with the department from my previous employer. I also thought that down the road we may have more than a rapid biopsy program so I should do a few larger samples at a regular processing schedule to validate both schedules (rapid and normal). I also thought that I should pick out a couple of blocks for representative special stains and IHC stains tha we would compare even though the new lab will send everything to the old lab for special stains and IHC stains. We have two processors and know I will also have to run a small validation of each new processor. I figured as I was validating the tissue processing programs I would also be validating the first tissue processor. Both of our two processors will be VIP6(s) and the machines from my former employer were also VIP6(s) which were of course validated. Can anyone share how many samples are adequate for tissue processing program validation and new processor validation? In other words what have you done and what was accepted by CAP since the wording of the question does not state any particulars? Also can you tell me if you think the special stains and IHC stains comparison is necessary, given that all of the stains will be done at the old lab and the only difference will be where the tissues were processed. My original design of the study had 25 parallel samples but I am wondering if that is overkill. Thanks Jim Vickroy Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Bone Saw
We also use the little table-top band saw from Mar-Med. It has a small footprint, splash guards, and is just awesome for cutting thin beautiful sections. We use toothed forceps to hold the specimen and keep fingers out of the way. Like the man said, a hot knife through soft butter! We have 3 Pathologists and one PA and they all LOVE it. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? We use a small band saw from MAR-MED. cuts through a femoral head like a hot knife through soft butter. You let the blade do the job, don't force the bone. Just very little consistent pressure. Rich Y - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: New Lab Setup
Hi Jim, While I do not have quite the length of time in the field that you do, I do have been through this several times and have some thoughts. First, to your question about how many samples is acceptable, I would say you should somewhat base this on your acceptability criteria. For example, if acceptability for your validation is 95% of the specimens being overall acceptable, you should do either 20 or 40 (for your scope, I would pick 20 personally), but NOT somewhere in between those numbers. The reason for this is you gain nothing in the land of percentages by doing, say 25. At 20, any more than one specimen that is unacceptable will throw you below 95%. At 40, you can have two outliers and still be within 95%. That is just a hypothetical, but hopefully you get the gist. Essentially, don't give yourself more opportunities to fail the validation if it gains you nothing in the margin. If your application requires a more robust validation, go for 40 (a la prognostic IHC). I would think an IHC/histochemistry validation would be overkill if you are validating your initial samples in parallel anyway. Just be sure to document that the results of the specimens were comparable to each other. I might have a different opinion if you were not using the exact same processors and doing some form of rapid/microwave processing instead. Regards, Bryan Date: Wed, 18 Feb 2015 17:27:50 + From: Vickroy, James jvick...@springfieldclinic.com Subject: [Histonet] New lab setup To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 9b1a1501a800064397369bd8072e6bca984...@e2k10db.springfieldclinic.com Content-Type: text/plain; charset=us-ascii I am working on setting up an adequate validation study for tissue processing at a new lab. I want it to be adequate but also not too labor intensive or costly. I have been in Histotechnology for over 36 years and have done this before in the lab I can from. Unfortunately sometimes I make things more complicated than others. I realize the standards for testing new protocols and antibodies are defined in the CAP checklist but routine tissue processing just says to document the procedure and to run parallel samples as a blind study. We are going to run only GI biopsies at first and I had planned on running parallel samples with the department from my previous employer. I also thought that down the road we may have more than a rapid biopsy program so I should do a few larger samples at a regular processing schedule to validate both schedules (rapid and normal). I also thought that I should pick out a couple of blocks for representative special stains and IHC stains tha we would compare even though the new lab will send everything to the old lab for special stains and IHC stains. We have two processors and know I will also have to run a small validation of each new processor. I figured as I was validating the tissue processing programs I would also be validating the first tissue processor. Both of our two processors will be VIP6(s) and the machines from my former employer were also VIP6(s) which were of course validated. Can anyone share how many samples are adequate for tissue processing program validation and new processor validation? In other words what have you done and what was accepted by CAP since the wording of the question does not state any particulars? Also can you tell me if you think the special stains and IHC stains comparison is necessary, given that all of the stains will be done at the old lab and the only difference will be where the tissues were processed. My original design of the study had 25 parallel samples but I am wondering if that is overkill. Thanks Jim Vickroy Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto: jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Bone Saw
What is clean-up like? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Wednesday, February 18, 2015 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bone Saw We also use the little table-top band saw from Mar-Med. It has a small footprint, splash guards, and is just awesome for cutting thin beautiful sections. We use toothed forceps to hold the specimen and keep fingers out of the way. Like the man said, a hot knife through soft butter! We have 3 Pathologists and one PA and they all LOVE it. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? We use a small band saw from MAR-MED. cuts through a femoral head like a hot knife through soft butter. You let the blade do the job, don't force the bone. Just very little consistent pressure. Rich Y - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Bone Saw
In the event you don't have a band saw, I wish I did, we have a vice that has a suction cup bottom that we attach to the top of one of our specimen carts. It holds the bone well enough to get reasonable sections. If I had to do too many more than I do, I'd request a band saw. When I'm done, I just clean up the saw, the cart and I'm done. Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow / CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, February 18, 2015 1:56 PM To: 'Terri Braud'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bone Saw What is clean-up like? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Wednesday, February 18, 2015 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bone Saw We also use the little table-top band saw from Mar-Med. It has a small footprint, splash guards, and is just awesome for cutting thin beautiful sections. We use toothed forceps to hold the specimen and keep fingers out of the way. Like the man said, a hot knife through soft butter! We have 3 Pathologists and one PA and they all LOVE it. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? We use a small band saw from MAR-MED. cuts through a femoral head like a hot knife through soft butter. You let the blade do the job, don't force the bone. Just very little consistent pressure. Rich Y - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2015.0.5646 / Virus Database: 4284/9137 - Release Date: 02/18/15 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for an article
Hi all, I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue. The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. Thank you for any help Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Help with cutting mouse brain at 9-10 Microns
Several questions: 1. Like Pamela has asked - what's the processing schedule and what regents are used? I've had some problems if the brain is soaked in EtOH too long (70% or higher), it will cause chattering. 2. Do you slice the whole brain including cerebellum and spinal cord or did you split the hemispheres to slice them individually? 3. Since you said that you cannot get any slices more than 9-10um at all, could you describe the situation? (i.e. can't get ribbons, it was cut unevenly such as thick and thin alternatively, paraffin too hard/too soft, etc) I mostly section rat and mouse brains at 10um without any problems. What I normally do is once the paraffin block (with brain inside of it, of course) is solidified, I tend to trim around the block around where the brain is and put it directly (the brain facing towards the ice) on the ice (NOT dry ice, just normal crushed ice) for 2-3 hours before sectioning it. After being on ice and right before sectioning, I like to rub with a small soft towel smooth the edges off around the brain then quickly dip it in the warm bath then section right away. I do not know why I always do those above, but it sure helped me every time I slice any rodent brains. Maybe that will help you somehow! Denise -- Message: 9 Date: Wed, 18 Feb 2015 14:20:26 + From: Kimberly Marshall kimbe...@animalreferencepathology.commailto:kimbe...@animalreferencepathology.com Subject: [Histonet] Help with cutting mouse brain at 9-10 Microns To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Message-ID: 1424269230196.97...@animalreferencepathology.commailto:1424269230196.97...@animalreferencepathology.com Content-Type: text/plain; charset=iso-8859-1 ?Hello Histo folks I am starting a research project with mouse brain, I am having trouble with chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all. I have soaked them in warm water and wonder in using a softener like conditioner would help. I am new to the animal tissue world and any advise would help. Thanks in advance. Kimberly -- The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Release of tissue
Thanks to all who responded to my requested for information concerning the release of tissue to patients. I seems that most facilities are not releasing tissue, especially if it has been fixed in formalin. Thanks, Gary Martin El Dorado Pathology Med. Grp. Marshall Med. Ctr. Pathology Dept. gmar...@marshallhospital.org mailto:gmar...@marshallhospital.org This e-mail is only intended for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other then the intended recipient is strictly prohibited. If you receive this communication in error, please contact the sender and destroy all forms of this communication. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening
We have a position open for Histology Surpervisor at Providence Hospital in Mobile, Alabama. Online application www.providencehospital.org. Anita Dudley Providence Hospital Mobile, al ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Looking for an article
You can read it here: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768083/ Geoff On 2/18/2015 2:48 PM, Fawn Bomar wrote: Hi all, I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue. The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. Thank you for any help Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcaul...@rwjms.rutgers.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking for an article
If you are a member of NSH you can also get a pdf for free by way of nsh.org. See email copied below. John Kiernan London, Canada = = = Dear All, I am pleased to confirm that the login button for the Journal of Histotechnology Editorial Manager site is now working. You may need to clear your internet browser cache before you can use the button. Instructions on how to do this are available at (http://www.wikihow.com/Clear-Your-Browser%27s-Cache; target=_blankhttp://www.wikihow.com/Clear-Your-Browser) My apologies for the inconvenience, With best wishes, Rasheda Begum Editorial Assistant Maney Publishing, 1 Carlton House Terrace, London, SW1Y 5AF, United Kingdom Tel: +44 (0)20 7451 7408 Fax: +44 (0)20 7451 7307 Email: r.be...@maneypublishing.com _ On 18/02/15, Morken, Timothy timothy.mor...@ucsf.edu wrote: If you are a member of NSH you can get a pdf for free just by contacting the staff at the NSH office. A great reason to join, if you have not already! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Wednesday, February 18, 2015 12:51 PM To: Fawn Bomar; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Looking for an article My error, I sent a link to a similar article. The original is in The Journal of Histotechnology. 2006;29(3):173-176 but my library does not have that journal. Perhaps someone on the list has it. Geoff On 2/18/2015 2:48 PM, Fawn Bomar wrote: Hi all, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking for an article
I sent her the .pdf file I downloaded from Many Pub. Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Wednesday, February 18, 2015 4:34 PM To: Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Looking for an article If you are a member of NSH you can also get a pdf for free by way of nsh.org. See email copied below. John Kiernan London, Canada = = = Dear All, I am pleased to confirm that the login button for the Journal of Histotechnology Editorial Manager site is now working. You may need to clear your internet browser cache before you can use the button. Instructions on how to do this are available at (http://www.wikihow.com/Clear-Your-Browser%27s-Cache; target=_blankhttp://www.wikihow.com/Clear-Your-Browser) My apologies for the inconvenience, With best wishes, Rasheda Begum Editorial Assistant Maney Publishing, 1 Carlton House Terrace, London, SW1Y 5AF, United Kingdom Tel: +44 (0)20 7451 7408 Fax: +44 (0)20 7451 7307 Email: r.be...@maneypublishing.com _ On 18/02/15, Morken, Timothy timothy.mor...@ucsf.edu wrote: If you are a member of NSH you can get a pdf for free just by contacting the staff at the NSH office. A great reason to join, if you have not already! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Wednesday, February 18, 2015 12:51 PM To: Fawn Bomar; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Looking for an article My error, I sent a link to a similar article. The original is in The Journal of Histotechnology. 2006;29(3):173-176 but my library does not have that journal. Perhaps someone on the list has it. Geoff On 2/18/2015 2:48 PM, Fawn Bomar wrote: Hi all, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking for an article
I would be curious to know if there was formalin on the processor. They only mentioned processing in a conventional manner. Just curious! Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow / CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Wednesday, February 18, 2015 3:47 PM To: Fawn Bomar; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Looking for an article You can read it here: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768083/ Geoff On 2/18/2015 2:48 PM, Fawn Bomar wrote: Hi all, I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue. The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. Thank you for any help Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcaul...@rwjms.rutgers.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2015.0.5646 / Virus Database: 4284/9137 - Release Date: 02/18/15 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help with cutting mouse brain at 9-10 microns
Hi Kimberly, Most of what I work with is mouse tissue and I've found the brains to be a bit tricky because they both hydrate and dry out quickly. I normally keep the paraffin blocks on an ice for about 2 hours (after they've been fully faced in), checking periodically to make sure the tissue is not overhydrating. When I section, I can normally only take a few sections before the brain starts to dry out again (you can tell when you start to see scratches and dry white areas on the tissue). Most of the blocks are ready to cut again after a few additional minutes back on the ice. For thicker sections (up to 20um), I take use the wooden stick of a cotton swab and hold it against the base of the paraffin block as I cut the section. The section will curl around the stick and you can roll it out flat on the water bath to smooth it out. Hope this helps! Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 301-846-1730 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone Marrow
How are you billing for bone marrow bxs. we do irons on smear and core plus clot. should we bill for all iron stains? also we do a wrights stain. Anita Dudley Providence Hospital Mobile, Al ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking for an article
If you are a member of NSH you can get a pdf for free just by contacting the staff at the NSH office. A great reason to join, if you have not already! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Wednesday, February 18, 2015 12:51 PM To: Fawn Bomar; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Looking for an article My error, I sent a link to a similar article. The original is in The Journal of Histotechnology. 2006;29(3):173-176 but my library does not have that journal. Perhaps someone on the list has it. Geoff On 2/18/2015 2:48 PM, Fawn Bomar wrote: Hi all, I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue. The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. Thank you for any help Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcaul...@rwjms.rutgers.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Looking for an article
My error, I sent a link to a similar article. The original is in The Journal of Histotechnology. 2006;29(3):173–176 but my library does not have that journal. Perhaps someone on the list has it. Geoff On 2/18/2015 2:48 PM, Fawn Bomar wrote: Hi all, I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue. The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. Thank you for any help Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcaul...@rwjms.rutgers.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking for an article
I'll scan a copy I have and email it to you later today. Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com www.parascreen.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, February 18, 2015 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for an article Hi all, I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue. The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. Thank you for any help Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Storing antibodies in a frostless freezer
Being old school I was taught that antibodies do not get stored in a frostless freezer. I am now being told that the new frostless freezers do not go above -13°C during the defrost cycle and it is safe to use. Teach this old dog a new trick, is anyone using a frostless freezer for storing antibodies? Jamie James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915mailto:858-812-1915jwat...@gnf.org jwat...@gnf.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help with cutting mouse brain at 9-10 Microns
?Hello Histo folks I am starting a research project with mouse brain, I am having trouble with chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all. I have soaked them in warm water and wonder in using a softener like conditioner would help. I am new to the animal tissue world and any advise would help. Thanks in advance. Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Help with cutting mouse brain at 9-10 Microns
Please give your processing schedule with times and reagents? It is hard to help without knowing how the tissue was handled from sacrifice to embedding. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly Marshall Sent: Wednesday, February 18, 2015 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help with cutting mouse brain at 9-10 Microns ?Hello Histo folks I am starting a research project with mouse brain, I am having trouble with chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all. I have soaked them in warm water and wonder in using a softener like conditioner would help. I am new to the animal tissue world and any advise would help. Thanks in advance. Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Looking for an article
This article about using honey as a fixative http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768083/ contends that you can use honey, jaggery sugar (look that up in Wikipedia), or sugar syrup as a fixative. What you're doing with honey is creating a transport medium - like the chemically more sophisticated Streck medium and others promoted to us a few years ago - that preserves the tissue until it can fix in alcohol in the processor. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] honey versus formalin
Sorry but my itchy delete finger got rid of the subscriber who was asking about this. There is a 2014 article by Sabarinath et al in Journal of Histotech. vol 37 pp 21-25. comparing honey and formalin fixation. If you use honey versus formalin as fixative and go to the go to www.maneyonline.com refernce you can access the entire article. Barry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
