[Histonet] 5-methylcytosine IHC in tissue
Hi Has anybody experience with 5-metcyt staining in brain tissue? some advice about the HCl and Boric acid treatment? I use 50um free floating sections ferfused with PFA 4%. Thank you in advance for your help!! Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Química Biológica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabellón II Piso 4 Ciudad Autónoma de Buenos Aires C1428EGA - Argentina Tel: 54 11 4576-3300/09 - Int. 221 email:marielachert...@gmail.com marielachert...@qb.fcen.uba.ar mariela.chert...@uab.cat ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Masson Trichrome stain
Justine, I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver. As a rule of thumb, it is just easier to use plastic all the way around. The Carson text does not state the use of only plastic forceps, but I would think that maybe they are concerned with a reaction between the Weigert's and the metal. That would be a stretch. As for no water before aniline blue, I believe the concentration is very weak and the water may dilute they dye even further. This would affect the staining results. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -- Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan jkier...@uwo.ca Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf lindamarg...@gmail.com, histonet@lists.utsouthwestern.edu Cc: justinelan...@hotmail.com Message-ID: 7380eaed48941.54fe3...@uwo.ca Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf lindamarg...@gmail.com wrote: Here is a message from Justine... From: Justine Lanzon [mailto:justinelan...@hotmail.com] justinelan...@hotmail.com] Sent: Thursday, March 05, 2015 5:36 AM To: lindamarg...@gmail.com Subject: Masson's trichrome stain Hi, I am doing a write up on Masson's trichrome stain however I cannot answer these two questions: - Why are plastic forceps used instead of metal ones to hold the stained slide? - Why do we not rinse before Alinine blue? ? Can you please help me? ? Many Thanks, Justine Lanzon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Old slides
We have literally about one hundred slides to re-slip for the this reason. Are there any suggestions for large numbers of slides to be re-coverslipped as this method would be too time consuming. We have used only glass for about nine years or so and it is much better. The old ones are the problem when someone needs THAT slide only. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Wednesday, March 11, 2015 10:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Old slides Bernice, Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so. Most of the bubbles will be gone, and the tissue will be saved. The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 + From: Bernice Frederick b-freder...@northwestern.edu Subject: [Histonet] Old slides. To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: eb9d7062461640e5ac22d213a4acc...@evcspmbx03.ads.northwestern.edu Content-Type: text/plain; charset=us-ascii Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Old slides
Bernice, Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so. Most of the bubbles will be gone, and the tissue will be saved. The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 + From: Bernice Frederick b-freder...@northwestern.edu Subject: [Histonet] Old slides. To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: eb9d7062461640e5ac22d213a4acc...@evcspmbx03.ads.northwestern.edu Content-Type: text/plain; charset=us-ascii Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 136, Issue 12
Hello, We received some liver tissue (Mouse) in 10% formalin (NFB). Then transferred to 70% ETOH. My question is that, Is it ok to transfer to 30% sucrose? So, frozen section can be perform. Please advise. Thanks, Bryan S. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, March 10, 2015 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 136, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Old slides. (Bernice Frederick) 2. RE: Old slides. (Jason McGough) 3. RE: Mushrooms for GMS fungus control (Morken, Timothy) 4. Re: FW: Masson's trichrome stain (John Kiernan) 5. RE: Old slides. (John Kiernan) 6. soft for microwriter (thermo scientific, Lamb, Shandon) (richard wild) 7. Re: Old slides. (b.curran.mcwill...@gmail.com) 8. Re: Old slides. (Rene J Buesa) 9. RE: Old slides. (Gowan,Christie C) 10. IHC / Morphometry Technician wanted in Shenandoah Valley Virginia (Erin Sarricks) -- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 + From: Bernice Frederick b-freder...@northwestern.edu Subject: [Histonet] Old slides. To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: eb9d7062461640e5ac22d213a4acc...@evcspmbx03.ads.northwestern.edu Content-Type: text/plain; charset=us-ascii Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu -- Message: 2 Date: Mon, 9 Mar 2015 14:20:09 -0600 From: Jason McGough jmcgo...@clinlab.com Subject: RE: [Histonet] Old slides. To: Bernice Frederick b-freder...@northwestern.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: zarafa.54fe0079.5165.2525b65b03dde...@mail.clinlab.com Content-Type: text/plain; charset=utf-8 Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com www.clinlab.com http://www.clinlab.com -Original message- From:Bernice Frederick b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu Sent: Monday, March 9, 2015 1:51 PM To: histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Mon, 9 Mar 2015 22:46:08 + From: Morken, Timothy timothy.mor...@ucsf.edu Subject:
[Histonet] Acetone fixing and tissue damage
Hi Everyone. When I fix my cryosections in acetone, I am using HPLC grade 99.9% for 10 minutes at -20C. Would the Histology grade 99.5% be less damaging to them? Higher H20 content, i.e. less than 99.5% apparently is also very bad. With the HPLC grade I often get tissue damage, the tissue also floats off the slide causing a stringy effect. Fixing with 4% p-formaldehyde or 100% Methanol, prevented the antibody from recognizing the Nuclear Antigens. Looking for advice, Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] repair 350-2 Thermo Scientific
The cyro console on our 350-2 Thermo Scientific is no longer cooling. Any ideas about getting this repaired and/or replaced?? We are located in central Florida. Thx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] THICK AND THIN SECTIONS ?
If you are using one of the following microtomes and are being told the advance mechanism is worn out. ( too much play between spindle and spindle nut ) You could be faced with purchasing a new Microtome. ( No parts availability ) REICHERT/JUNG 2030 LEICARM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 Rather than replacing these excellent Instruments, I have a PERMANENT solution to fix this problem. For Information, contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.der...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diff Quik troubleshooting
Good Morning- Random FNA case where the slides turn blue as they dry. We have tried taking them back through stains, destaining and restaining, etc. FNA smears are air-dried when we receive them on plain slides, unfixed. Any thoughts appreciated. Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
