[Histonet] FT NIGHT (evening shift) POSITION DELRAY BCH FL
Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com if interested. *full time position Mon-Fri or Sun-Thurs 6PM-2:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of your shift) *MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred Kari M Simeone 561.819.6517 fax ksime...@leavittmgt.commailto:ksime...@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Special Stain kits Vet lab ques?
This is an SOP question. I have several older kits that I have made up in-house solutions to replenish certain stains that have been used up without having to order a whole new kit. (eg. decolorizer) How do word the Stain SOP to be put into practice? How often will I have to revise it officially until a new kit is purchased? If the stain is coming out correctly, will this pass ISO standards? Thanks for any help Fran ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Dako Artisan Special Stainer
Hi all, We recently upgraded our old Artisan to the Artisan Link Pro. It does the depar. within the system itself. Set-up was easy, we just transferred our old protocols to the new units. Modifying protocols was easy too. Staining is consistent, tech support and their specialists are reliable and share information from what other users do. If I have kit problems, a replacement kit is shipped overnight to the lab. Kits come in 50 test or 100 test, our GMS 100 test kit usually lasts us 1 month and staining is consistent. We run multiple protocols per run and found that certain combinations can go together to save time such as: GMS, Reticulin and Jones for a 1 hour 20 minute stain and Trichrome, PAS to 1 hour, 40 minutes. Last month, we did 369 stains and 347 stains in May on this system. I print out usage reports by protocols and case lists including which unit performed the stain. A quick scan of the barcode lets me know the lot number and expiration date of the kit used. Hope this was helpful. Jose de Guzman MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected. Thank you. Help conserve valuable resources - only print this email if necessary. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hi Need some advise on tissue cassette printers. What is everyone using, and what problems does everyone have ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re. Decalcification with formic acid sodium
Dorothy and Carl, Comments about your Histonet replies on formic acid decalcification. The Morse solution referred to by Dorothy can be picked up online by typing in the DOI number: 10.1.1.4689.3439.pdf or title,Morse A. Formic acid-sodium citrate decalcification and butyl alcohol dehydration of teeth and bones for sectioning in paraffin. 1945 J Dental Res 1945:24:143. You will find the reference to Evans and Krajian paper on formic acid/sodium citrate along with the original recipe for their solution (equal parts of 85% (stock) formic acid and 20% sodium citrate). Morse modified the Evans Krajian method (1 part diluted stock formic acid i.e. 90% diluted 1:1 with water for 45% formic acid) plus 1 part 20% sodium citrate. The Morse paper was excellent and well worth reading. Interestingly, in 1962, our lab used the Morse solution for decalcifying teeth although it was never referred to by that name but simply formic acid/sodium citrate. The formic acid content in Morse's solution is half the concentration of formic acid in Evans/Krajian solution. It seems both work equally well and the higher concentration of formic acid should increase the decalcification rate somewhat. Morse also did chemical decalcification endpoint testing. Carl is correct about not mixing citric acid with formic acid as citric acid is not going to act as a buffer salt. However, you will find in the literature that citric acid is very mild and has been used as a decalcifying agent for EM studies. Carl is also correct in that sodium formate can be used as a buffering salt instead of sodium citrate. We have worked with both of the buffering salts/formic acid formulations and found they works equally well for decalcification. I have some publications on file comparing acid versus EDTA for cartilage and IHC studies and learned some researchers referred to buffered formic acid methods as acidic buffers . The latter terminology could be confusing to people in the business of decalcifying bones and teeth. but no more so than the acronyms manufacturers give their solutions. It pays to read the MSDS for any decalcifying solution, and even compare this information to what is in histology textbooks as part of our education. I have found the discussions on this topic enlightening.I will be happy to send the pdf of the Morse method to those interested in reading it. I have not been able to access the 1930 Evans Krajian method yet. What is important is knowing these older, classic formic acid methods are still tried and true with the added advantage of being available commercially for our convenience. Thanks everyone Gayle Callis You Wrote: There was a paper http://www.genedetect.com/Merchant2/ExampleRefs/Decalcifying_protocols.pdf http://www.genedetect.com/Merchant2/ExampleRefs/Decalcifying_protocols.pdf Talking about formic acid (Morse solution) can get as good result as EDTA in ISH. FYI. Dorothy Hu Mouse knee joints: done lots of decalcified FFPWS for assessment of articular cartilage degeneration models. See Histonet images for a Tol blue image. Decal in 10 % EDTA for 3 days on a rocker at RT. Sure5days if you are worried. No difference in Immuno-reactivity, imho. If you want to use buffered Formic acid, use Formic acid; sodium formate. Use of citric acid with Formic acid does not make a buffer. It's just mixing two relatively mild acids. However, I am sure that Prof Kiernan can further enlighten us. Respectfully, Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Toluidine blue stain for MMA
Hi, is there anyone have an experience with MMA toluidine staining? Im using T7200, T9100, Osteo-bed resin in lab now. Thanks, Kai Research Histotechnologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Toluidine blue stain for MMA
Yes. Probably hundreds of Histonetters stain plastic sections. Let us all hope they don't all bombard the Histonet listserver with replies to your question. Instructions for staining plastic sections with toluidine blue are in every library that contains books with paper pages, and also (albeit with less authority) in great abundance on the Web. Try typing SEMITHIN STAIN into Google. I just did, and an excellent web site came up on top of the heap. John Kiernan = = = On 27/07/15, Kai Hong via Histonet histonet@lists.utsouthwestern.edu wrote: Hi, is there anyone have an experience with MMA toluidine staining? Im using T7200, T9100, Osteo-bed resin in lab now. Thanks, Kai Research Histotechnologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Dako Artisan Special Stainer
Thank you! All for your feedback. It will definitely help us make better decision for our lab. In addition to quality user friendly, we need this to work with our LIS / vantage tracking system...need it IT friendly too! Nothing is plug and play anymore... Kiran On Jul 27, 2015, at 11:27 AM, deGuzman, Jose R via Histonet histonet@lists.utsouthwestern.edu wrote: Hi all, We recently upgraded our old Artisan to the Artisan Link Pro. It does the depar. within the system itself. Set-up was easy, we just transferred our old protocols to the new units. Modifying protocols was easy too. Staining is consistent, tech support and their specialists are reliable and share information from what other users do. If I have kit problems, a replacement kit is shipped overnight to the lab. Kits come in 50 test or 100 test, our GMS 100 test kit usually lasts us 1 month and staining is consistent. We run multiple protocols per run and found that certain combinations can go together to save time such as: GMS, Reticulin and Jones for a 1 hour 20 minute stain and Trichrome, PAS to 1 hour, 40 minutes. Last month, we did 369 stains and 347 stains in May on this system. I print out usage reports by protocols and case lists including which unit performed the stain. A quick scan of the barcode lets me know the lot number and expiration date of the kit used. Hope this was helpful. Jose de Guzman MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected. Thank you. Help conserve valuable resources - only print this email if necessary. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
