[Histonet] Reagent bottles VIP 5

[Hilllel Hellinger via Histonet]

I need used reagent bottles for VIP 5
Does anyone have?

-- 
Hillel Hellinger
North Miami Beach, Fl 33162
Tel# 305-992-1348
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[Histonet] Image analysis software

[Judi Ford via Histonet]

Hi everyone,
We are in the middle of looking at image analysis software and I was wondering 
what software programs people are using. Why did you pick the one you are 
using? Are you using it for brightfield whole slide analysis or for fluorescent 
slide analysis?  What file types can be used with your software?

I would love to hear your experiences.

Thanks,
Judi
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Re: [Histonet] Professiional competency

[Megan Dishop via Histonet]

Fawn, 

At Children's Minnesota, this is managed and tracked at the hospital level via 
ongoing and focused professional practice evaluation (OPPE, FPPE).  We have 
chosen specific elements of our anatomic pathology quality program to forward 
to the hospital and then these are incorporated into a semi-annual assessment 
form, and reviewed by a hospital committee.

Our internal laboratory policy reflects this practice and lists some of the 
specific quality metrics we have chosen, but your lab leadership and pathology 
group should select criteria that seem most appropriate for your practice 
setting.  See our attached policy as an example.



Megan K. Dishop MD
Medical Director, Pediatric Anatomic Pathology
Children's Hospitals and Clinics of Minnesota Laboratories
2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA
Phone: 612-813-6521Fax: 612-813-7721  Email: megan.dis...@childrensmn.org
Adjoint Professor of Pediatrics, University of Colorado School of Medicine




>>> Fawn Bomar via Histonet  2/25/2016 12:39 
>>> PM >>>
Does anyone have a policy for assessing professional competency and a log for 
recording the assessment for pathologists who provide interpretive services to 
the lab that they would be willing to share?



Thank you

Fawn
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[Histonet] Professiional competency

[Fawn Bomar via Histonet]

Does anyone have a policy for assessing professional competency and a log for 
recording the assessment for pathologists who provide interpretive services to 
the lab that they would be willing to share?



Thank you

Fawn
-
This electronic message may contain information that is 
confidential or legally privileged.  It is intended only
for the use of the individual(s) and entity named as recipients
in the message. 

If you are not an intended recipient of this message, please 
notify the sender immediately and delete the material from any 
computer. Do not deliver, distribute, or copy this message, and 
do not disclose its contents or take any action in reliance on
the information it contains. 

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[Histonet] metal enhanced DAB

[King,Michael A via Histonet]

Tyrone,
I've had better luck using acetate buffer with metal-enhanced DAB.  There's 
less of a precipitation problem.  You can also dilute the reaction solution and 
the reaction product will develop more slowly, or just decrease the peroxide 
concentration.  You also have some control over the color by adjusting the pH; 
Co can range from blue to black and Ni from brown to black. 
Good luck,
Mike King

-
Wed, 24 Feb 2016 16:08:00 -0600 Tyron Genade wrote:
Subject: [Histonet] metal enhanced DAB
Message-ID:

[Histonet] Rabbit and chicken eye processing

[mohamed abd el razik via Histonet]

Dear all 
What is your optimal protocol for processing rabbit and chicken eyes ? and do 
you prefer to cut it 2 halfs or inject it with NBF 10% or whole processing is 
the best ??
Thanks in advance
Mohamed
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Re: [Histonet] Human nuclear antibody MAB1281

[Eva Permaul via Histonet]

Liz,
Any chance you could find out what protocol was used by the ones that have
used it? or help me find some of the literature references? Everywhere I
look I just see people who have not gotten it to work asking for help.
Thanks,
Eva

On Thu, Feb 25, 2016 at 10:39 AM, Elizabeth Chlipala 
wrote:

> Eva
>
> We have worked with various antibodies in order to detect human cells in a
> rodent background.  I know that this antibody has been referenced in the
> literature and I also have known of other individuals who have had success
> with it, but in our hands we have never been able to make it work on FFPE
> tissues but I believe it works in frozen sections.  We have opted to try
> some different antibodies.
>
> NuMA -we researched this protein and determined that it may work -here is
> a snippet of what we found from a quick look into the Human Protein Atlas
> entry for NUMA1. Though it is functionally involved in the structural
> rearrangement of DNA during mitosis and apoptosis, it is a constitutively
> expressed structural protein. From the images on HPA (
> http://www.proteinatlas.org/ENSG0137497-NUMA1/tissue/primary+data)
> it looks like it is almost universally expressed, with the one exception of
> hepatocytes. The vast majority of neurons, which are overwhelmingly
> post-mitotic, show fairly strong expression, so that's encouraging.   We
> initially looked at the cell signaling antibody (our target was human cells
> in a rat background) but found that it does cross react with rat, its fine
> with mouse but this antibody is our hands was found to be sensitive to time
> in fixation and retrieval time and temp.  We opted to try another source
> for this antibody - abcam.   They had several different antibodies for that
> target.  We work with their antibodies a lot and they normally do not list
> the sequence of the antigen so we could not check the sequence homology to
> other species ourselves but you can call them and they will provide that
> information.  Here is what they provided to us -   "We have not tested
> ab86129 for cross reactivity with rat and we have not received any
> researcher feedback for using the antibody with this species. The immunogen
> sequence has 56% identity with the rat protein, so it is unlikely that it
> will cross react. However we have not confirmed this experimentally and we
> do not know if it would show non-specific staining with rat. I do think
> ab86129 would be the best antibody to try since it has the lowest homology
> with the rat protein. The other antibodies that have not been tested with
> rat have 69% (ab55767) or 88% (ab84680) homology."  Ab86129 worked very
> nicely in our hands.
>
> HLA-1 this will also work nicely but the protein is expressed in the
> cytoplasm and expression levels can vary but it does work nicely in some
> instances.
>
> So for us its dependent upon the project as to which antibody we will use
> but the NuMA is expressed in the nuclei so it will work quite nicely for
> dual staining of another protein that is expressed in the cytoplasm or cell
> membrane.
>
> Good Luck
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> l...@premierlab.com
> www.premierlab.com
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> -Original Message-
> From: Eva Permaul via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Thursday, February 25, 2016 7:07 AM
> To: histonet
> Subject: [Histonet] Human nuclear antibody MAB1281
>
> Good morning,
> We recently bought MAB1281 with the hope of being able to determine if the
> cells in a mouse model was human or mouse.
> We want to use it to stain FFPE tissues. I have tried it with Citrate per
> the companies recommendation. I ran it on a human tonsil but saw no
> staining at all.
> Is there anyone who has been able to get this antibody to work? And if so
> would you please share your protocol?
> Thank you,
> Eva
> ___
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Re: [Histonet] Human nuclear antibody MAB1281

[Elizabeth Chlipala via Histonet]

Eva

We have worked with various antibodies in order to detect human cells in a 
rodent background.  I know that this antibody has been referenced in the 
literature and I also have known of other individuals who have had success with 
it, but in our hands we have never been able to make it work on FFPE tissues 
but I believe it works in frozen sections.  We have opted to try some different 
antibodies. 

NuMA -we researched this protein and determined that it may work -here is a 
snippet of what we found from a quick look into the Human Protein Atlas entry 
for NUMA1. Though it is functionally involved in the structural rearrangement 
of DNA during mitosis and apoptosis, it is a constitutively expressed 
structural protein. From the images on HPA 
(http://www.proteinatlas.org/ENSG0137497-NUMA1/tissue/primary+data)  it 
looks like it is almost universally expressed, with the one exception of 
hepatocytes. The vast majority of neurons, which are overwhelmingly 
post-mitotic, show fairly strong expression, so that's encouraging.   We 
initially looked at the cell signaling antibody (our target was human cells in 
a rat background) but found that it does cross react with rat, its fine with 
mouse but this antibody is our hands was found to be sensitive to time in 
fixation and retrieval time and temp.  We opted to try another source for this 
antibody - abcam.   They had several different antibod
 ies for that target.  We work with their antibodies a lot and they normally do 
not list the sequence of the antigen so we could not check the sequence 
homology to other species ourselves but you can call them and they will provide 
that information.  Here is what they provided to us -   "We have not tested 
ab86129 for cross reactivity with rat and we have not received any researcher 
feedback for using the antibody with this species. The immunogen sequence has 
56% identity with the rat protein, so it is unlikely that it will cross react. 
However we have not confirmed this experimentally and we do not know if it 
would show non-specific staining with rat. I do think ab86129 would be the best 
antibody to try since it has the lowest homology with the rat protein. The 
other antibodies that have not been tested with rat have 69% (ab55767) or 88% 
(ab84680) homology."  Ab86129 worked very nicely in our hands.

HLA-1 this will also work nicely but the protein is expressed in the cytoplasm 
and expression levels can vary but it does work nicely in some instances.

So for us its dependent upon the project as to which antibody we will use but 
the NuMA is expressed in the nuclei so it will work quite nicely for dual 
staining of another protein that is expressed in the cytoplasm or cell membrane.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Eva Permaul via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, February 25, 2016 7:07 AM
To: histonet
Subject: [Histonet] Human nuclear antibody MAB1281

Good morning,
We recently bought MAB1281 with the hope of being able to determine if the 
cells in a mouse model was human or mouse.
We want to use it to stain FFPE tissues. I have tried it with Citrate per the 
companies recommendation. I ran it on a human tonsil but saw no staining at all.
Is there anyone who has been able to get this antibody to work? And if so would 
you please share your protocol?
Thank you,
Eva
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[Histonet] (no subject)

[Nirmala Srishan via Histonet]

Hi All. 

If anyone is doing the ER/PGR Immnunohistochemistry TMA with CAP, what 
have you been doing when when they grade some cores as code 27 ?  If any 
one has any input regarding this, I would greatly appreciate it. 
CAP provide the slides, but one of our slides were missing some cores.

Thanks

Nirmala










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Re: [Histonet] paraformaldehyde

[Caroline Miller via Histonet]

I don't know if there is a hard and fast rule, but I keep 20% in the fridge
for around 6 months or until the little bits start to form on the bottom.
4% I would never keep that long, maybe a week at most.

Best if you want fresh, small volumes, all the time is to get those little
37% ampules and dilute as necessary

I am very interested to hear other folks opinions too, as I am sure they
will vary!

yours,
mills

On Thu, Feb 25, 2016 at 6:11 AM, Bruijntjes, J.P. (Joost) via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi all
>
> Can anyone tell me what the expiration time is for home-made
> paraformaldehyde?
>
> Joost
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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[Histonet] RELIA HOT JOB ALERT New Opportunities in AZ, NY, VA and TX

[Pam Barker via Histonet]

Hi Histonetters!
I hope your week is going well and you are getting ready for a fantastic
weekend.
My phone continues to ring OFF the hook and here are the latest exciting
opportunities:
Histotech - Flagstaff, AZ
Immunohistochemistry Specialist - Long Island, NY
Histology Manager - Amarillo, TX
Lead Histotech - Norfolk, VA

I also have exciting opportunities in CA, WI, KY, AR and MT

All of these positions are full time and permanent and my clients offer
relocation assistance and/or sign on bonuses.
For more information please contact Pam Barker toll free at 866-607-3542 or
cell/text 407-353-5070 or email at rel...@earthlink.net 

Thanks and have a great day!

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 






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[Histonet] Human nuclear antibody MAB1281

[Eva Permaul via Histonet]

Good morning,
We recently bought MAB1281 with the hope of being able to determine if the
cells in a mouse model was human or mouse.
We want to use it to stain FFPE tissues. I have tried it with Citrate per
the companies recommendation. I ran it on a human tonsil but saw no
staining at all.
Is there anyone who has been able to get this antibody to work? And if so
would you please share your protocol?
Thank you,
Eva
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[Histonet] paraformaldehyde

[Bruijntjes, J.P. (Joost) via Histonet]

Hi all

Can anyone tell me what the expiration time is for home-made paraformaldehyde?

Joost
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[Histonet] cassette printer

[Maryann Deathridge via Histonet]

Hi Histoland-
 Looking for a refurbished cassette printer. Currently using the Shurmark 
cassette printer.  Prefer several hoppers for color cassettes, ability to 
print on Activ-Flo cassettes and Simport cassettes with no issues. At this 
point if it can just print.
 Need something to get through till we make a decision on the complete 
"system" (specimen tracking) for our lab.
 Thanks
  
  
  Maryann Deathridge, BS HT (ASCP)  Pathology Assoc. of St. Thomas  4220 
Harding Pike, Bldg. SE, Suite 504  Nashville, TN 37205  (615) 298-4100  
madeathri...@pastnashville.com  

  




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