Re: [Histonet] automatic stainers and tissue processors

[WILLIAM DESALVO via Histonet]

I suggest you take the opportunity to find the instruments that fit your needs. 
Are going to rapid process, what types of tissue samples do you have, what do 
you want to improve? The histology lab is changing and you should look 5+ years 
down the road. I would not want you to be pigeon holed into a set of 
instruments. Drive quality, patient experience and flexibility. I am a wrong 
proponent of rapid processing, fix appropriately and do not use xylene or over 
process. For staining, Prisms and film are my choices. You need to find the 
instrumentation that works best for you. Good luck in your search, there are 
many options and great products to evaluate.

Sent from my Windows Phone

-Original Message-
From: "Silvia Bonner via Histonet" 
Sent: ‎2/‎29/‎2016 1:19 PM
To: "histonet@lists.utsouthwestern.edu" 
Subject: [Histonet] automatic stainers and tissue processors


Hello,
We are looking into purchasing new automatic H strainers and new tissue 
processors.  Any helpful advice?  I know this may have been discussed before 
but I an new to histonet.
Thanks for your help!
Silvia Bonner, HT(ASCP) CM
Histology Supervisor

sbon...@pathregional.com
Pathologists' Regional Laboratory
1225 Highland Ave
Clarkston, WA 99403
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[Histonet] MET amplification - need help

[Mark Tarango via Histonet]

Hello everyone,

I am working a validation for MET amplification by FISH and am having a
hard time finding positive cases.  Would anyone have any to share or
trade?  I have access to all kinds of cases from both IHC and molecular for
exchange.

thanks

*Mark Tarango*
Lead Molecular Technologist - FISH

CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-576-6526
Fax: 206-215-5946.
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Re: [Histonet] H staining

[Tony Henwood (SCHN) via Histonet]

I would look at the dewaxing stage.

Have you changed waxes?
Try longer in xylene &/or longer in the oven.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, 29 February 2016 10:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H staining

Hello all,

 We've been running into an issue lately with our H staining. The hematoxylin 
and eosin are light on and off but the chromatin is consistently staining 
light. I have changed the time in hematoxylin to longer, used a fresh lot of 
both hematoxylin and our clarifier solution and the stain is not improving.  
Does anyone have any suggestions?

Here is our routine stain line procedure.
65 degree Celsius oven for 20 minutes
2x xylene for 5 mins
2x 100% alcohol for  1 minute
95% alcohol for 1 minute
DI water rinse for 1 minute
Richard Allan scientific hematoxylin for 4 minutes DI water rinse for 2 minutes 
Richard Allan Clarifier 1 for 2.5 minutes DI water for 2 minutes Bluing Reagent 
for 1 minute DI water rinse for 2 minutes 95% alcohol for 1 minute Eosin Y for 
2 minutes 95% alcohol for 30 seconds 2x 100% alcohol for 1 minute each 2x 
xylene for 1 minute each Mount and coverslip.
-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] H staining

[Teri Johnson via Histonet]

Hi Charles,

If my memory serves, Clarifier 1 and Clarifier 2 are different mostly in that 
one is an alcoholic solution and the other an aqueous one. So they both behave 
differently in their mechanism of action. The alcohol based reagent should 
remove more hematoxylin from the cells than the aqueous based reagent.

Also if you do a google search for Richard Allan Clarifier 1, you should find a 
pdf document that outlines what their stain series are, and how to use (time) 
them. It's a great document that has a couple charts on how to increase or 
decrease stain contrast.  It appears that they recommend 30 seconds-1 minute 
for either solution for the Hematoxylin 7211 and 7212 stain, and 20 seconds-1 
minute if using the Hematoxylin 1 and 2. At 2.5 minutes, you might be overdoing 
your "clarifying"!

Best wishes and good luck!

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohn...@genoptix.com
www.genoptix.com




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Re: [Histonet] automatic stainers and tissue processors

[Jamal Rowaihi via Histonet]

Go for Sakura products with no hesitate. 


Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent 
from my cell phone Original message From: Silvia Bonner via 
Histonet  Date: 2/29/2016  11:09 PM  
(GMT+03:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automatic 
stainers and tissue processors 

Hello,
We are looking into purchasing new automatic H strainers and new tissue 
processors.  Any helpful advice?  I know this may have been discussed before 
but I an new to histonet.
Thanks for your help!
Silvia Bonner, HT(ASCP) CM
Histology Supervisor

sbon...@pathregional.com
Pathologists' Regional Laboratory
1225 Highland Ave
Clarkston, WA 99403
 This email may contain physician, patient and/or attorney material that is 
confidential or privileged for the sole use of the intended recipient. YOU ARE 
NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF 
THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED.  If you 
are not the intended recipient, please notify us immediately by telephone at 
(208) 746-0516 or (509) 758-5576 and delete all copies.

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[Histonet] automatic stainers and tissue processors

[Silvia Bonner via Histonet]

Hello,
We are looking into purchasing new automatic H strainers and new tissue 
processors.  Any helpful advice?  I know this may have been discussed before 
but I an new to histonet.
Thanks for your help!
Silvia Bonner, HT(ASCP) CM
Histology Supervisor

sbon...@pathregional.com
Pathologists' Regional Laboratory
1225 Highland Ave
Clarkston, WA 99403
 This email may contain physician, patient and/or attorney material that is 
confidential or privileged for the sole use of the intended recipient. YOU ARE 
NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF 
THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED.  If you 
are not the intended recipient, please notify us immediately by telephone at 
(208) 746-0516 or (509) 758-5576 and delete all copies.

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[Histonet] Masson's Trichrome or Sirius Red on frozen tissue

[Connolly, Brett M via Histonet]

Has anyone preformed these stains of frozen sections??? Looking for any tips.

I'll have fresh frozen tissue that can be fixed after sectioning and mounting 
onto the slide.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

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Re: [Histonet] Freeze Spray Ban

[Charles Riley via Histonet]

We use Stoner Multipurpose freeze spray. 100% pure virgin tetrafluoroethane

On Mon, Feb 29, 2016 at 12:29 PM, Histology via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi All,
>
> Just got an alert stating that the EPA is banning HFCs found in Freeze
> Spray effective July 20th.  Does anybody use a spray that does not contain
> this banned chemical?  If so, please let me know where you get it.
>
> Thanks,
>
> Mehndi Helgren
> Dominion Pathology Labs  "DPL"
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-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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[Histonet] Freeze Spray Ban

[Histology via Histonet]

Hi All,

Just got an alert stating that the EPA is banning HFCs found in Freeze Spray 
effective July 20th.  Does anybody use a spray that does not contain this 
banned chemical?  If so, please let me know where you get it.

Thanks,

Mehndi Helgren
Dominion Pathology Labs  "DPL"
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[Histonet] NJ Histology Meeting in June

[FRENCH, MICHELE via Histonet]

Dear Histonet Members,

I wanted to let you know that The New Jersey Society for Histotechnology is 
planning to host a two-day Summer Meeting June 10-11 at the Wyndham Hotel in 
Mount Laurel, NJ. More information will be available at the end of March, and 
meeting registration will start on April 1st.

SPEAKERS NEEDED ASAP: We need to fill out last three time slots: (one 1.5hr 
session, one 3hr session and one 45minute session).
VENDORS NEEDED: We only have 14 tables left in the exhibit area. Reservations 
are on a first-come, first-served basis.
JOB LISTINGS WANTED: If you have a job opening that you would like posted on 
our Employment Opportunities board during the event, just let us know!

Please contact Michele French by Email 
michele.fre...@bms.com or Phone 609-818-3873.


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[Histonet] H staining

[Carlos Defeo via Histonet]

Charles,extend time on the deparaffinization station,and even 
better,renew solvents at this point also.

My kind regards,
Carlos Defeo
Histotechnologist
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