We have always stored fixed specimens of various sizes in plastic Nalgene
bottles, because they are so resistant to chemicals. However, we're recently
realized that the bottles grow very brittle with age, and can "explode" into
numbers shards when squeezed slightly. I think it is a result of
We purchase the antibody from Ventana and run it on our Bond Max platforms.
During re-optimization, we found we could dilute it 1:10 (15 minutes) following
"H2" (high pH) retrieval and still get excellent results.
Richard
Richard W. Cartun, MS, PhD
Director, Histology and the Martin M.
Hello Histonet,
Our exclusively retained client has opened a new Applications Specialist
opportunity and I wanted to reach out regarding a few specifics and see if
anyone would be interested in learning more.
Our client is a global leader in cancer diagnostics and they are looking
for an
Ventana medical systems is the only one I know of right now.
On Wed, Jun 8, 2016, 10:47 AM Baldwin, Kathy via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Just saw someone reaching out for P16 was wondering if anyone would share
> their protocol for this antibody we are using
Annual Formaldehyde safety education should be part of your on-boarding
process, as well as annual education, per OSHA
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
4.
Jim,
You are correct. OSHA requires annual education for any personnel that handle
formalin to be educated in the hazards, proper handling, etc... This can be as
simple or as involved as you want to make it but it should cover the basics at
the very least.
Thanks,
Julia Cates, HT(ASCP)cm
Just saw someone reaching out for P16 was wondering if anyone would share their
protocol for this antibody we are using Ventana/Roche and are stain is coming
out weak :(
Thanks
S. Kathy Baldwin
Histology/Cytology Supervisor
Memorial Hospital and Health Care Center
800 West 9th St.
Jasper,
Ventana/Roche bought out MTM which had the patent, so they're the only vendor
you can get it from. Since you're using Bond you obviously don't want it in a
dispenser, but you can buy their manual staining kit and just use the antibody
from that.
Michael J. Dessoye, M.S. |
We use the p16 from Ventana/Roche, but of course it comes in their dispenser.
Jeanne Blanchard, CT (ASCP)CM
-Original Message-
From: Paula via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, June 08, 2016 11:14 AM
To: histonet@lists.utsouthwestern.edu
Subject:
Hello,
Our pathologist would like us to get the P16 antibody. We have the Bond III
and we use ready to use antibodies. Leica doesn't carry this antibody and so
I was hoping someone can tell me which vendor carries it and that I can
order it from.
Thanks in advance for your help
Paula
Lab
Pretty interesting Erin, thanks. I reread the Nature article I saw back in
February detailing a bit about the complaints being investigated. Among those
complaints were notifications of goats with coyote bites and massive tumors
(from where??). Both of which could drastically affect
Hi all!
Santa Cruz Biotech has to discontinue all goat and rabbit antibody production:
http://blogs.sciencemag.org/pipeline/archives/2016/05/23/trouble-at-santa-cruz-biotechnology
I hope you are all having a great Tuesday!
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology
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