[Histonet] Technovit 9100 PMMA Depolymerization
Hello, any information on how to depolymerize bone samples that are in Technovit 9100 Poly(methyl methacrylate) PMMA polymer? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Vacuolated and torn?
If it is a block issue you can also often see 'white' bits on the block surface that correlate to the torn areas on the slide, try 'polishing' the block at 1um until they go away, cool and then try sectioning again. I am unsure how that relates to vacuolation - as I have usually seen that referred to an intracellular event - and not to refer to a regular hole. I also suggest having someone else cut a block and then compare. yours, mills On Thu, Oct 6, 2016 at 3:43 PM, Cartun, Richard via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Microtomy is so important, but often over-looked. You might try giving > the paraffin block(s) to different people and have them all cut H, and > then have your pathologist compare them. If everyone's sections are > suboptimal, then the problem is with fixation and/or processing. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > > -Original Message- > From: Martin, Erin via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Thursday, October 06, 2016 9:31 AM > To: histonet > Subject: [Histonet] Vacuolated and torn? > > Good morning everyone! > > One of my pathologists says that we are having a problem with the tissue > on the slides looking vacuolated and torn. He is convinced it is from > microtomy. Anyone have any ideas? I was thinking that it might be a > processing issue. > > > > Thanks in advance! > > Erin Martin, Histology Supervisor > UCSF Dermatopathology and Oral Pathology Service > 1701 Divisadero St, San Francisco, CA 94044 > 415-353-7248 > > Confidentiality Notice > The information transmitted is intended only for the person or entity to > which it is addressed and may contain confidential and/or privileged > material. Any review, retransmission, dissemination or other use of, or > taking of any action in reliance upon, this information by persons or > entities other than the intended recipient is prohibited. If you receive > this in error please contact the sender and delete the material from any > computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Vacuolated and torn?
Microtomy is so important, but often over-looked. You might try giving the paraffin block(s) to different people and have them all cut H, and then have your pathologist compare them. If everyone's sections are suboptimal, then the problem is with fixation and/or processing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: Martin, Erin via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, October 06, 2016 9:31 AM To: histonet Subject: [Histonet] Vacuolated and torn? Good morning everyone! One of my pathologists says that we are having a problem with the tissue on the slides looking vacuolated and torn. He is convinced it is from microtomy. Anyone have any ideas? I was thinking that it might be a processing issue. Thanks in advance! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura/Tissue Tek Glas Coverslipper
Hi all, I'm curious if anyone uses a Sakura/Tissue Tek Glas Coverslipper in their lab, and if they use xylene/xylene based media or if it would be possible to use a xylene substitute product on the machine? Thanks Tyanna Stuckey Histology Technician North Sound Dermatology tyanna.stuc...@northsoundderm.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Handling Breast Lumpectomy Specimens with radioactive seed localization
Further researching the topic, I've learned that there are two recently introduced non-radioactive alternatives, Savi Scout and Sentimag. I don't understand how they work. Coincidentally, I received a circular in this morning's mail with information about a continuing medical education event on breast disease, with presentations about both of these non-radioactive techniques. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Faxitron
Hi, We use a Faxitron machine in our lab. _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. -Original Message- From: Lori Wright [mailto:wrg...@aol.com] Sent: Wednesday, October 05, 2016 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Faxitron Hi All, I was wondering if anyone was using a Faxitron in their Lab? Thanks, Lori Wright Lead Technician Surgical Pathology Duke Medicine Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cutting blocks for mouse tissue troubleshooting
Blanca If your samples are fixed and processed properly the sections should cut without wrinkles. We process mouse pancreas on a 30 minute cycle - 30 minutes per station starting at 50% alcohol. We trim and soak blocks on ice and we normally do not have issue with wrinkles. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting blocks for mouse tissue troubleshooting Hello Histonets! I need to find out a better way to cut the whole pancreas mouse tissue without wrinkles, Does anybody has suggestion. I am having trouble to obtain a nice section without wrinkles. It is pretty big piece and crawl in on my embedding, is hard to stretch out without breaking. I cannot use ammonia water because they use the unstained for research and I don't want get discrepancies in any other stains. Suggestions??? thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: blanca.lo...@utsouthwestern.edu UT Southwestern Medical Center The future of medicine, today. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Small Auto cover slipper to use with Histoclear.
Hello Histonet! I work in a lab that uses Histoclear as our clearing agent on our automatic stainer. I am looking for an automatic cover slipper/cover slipping tape that we can use with this. The thing about our lab is that it is very small and we have limited space. If there is not something out there for us, would it be possible to use xylene on only the cover slipper to avoid changing our protocols? Thanks! Tyanna Stuckey Histology Technician North Sound Dermatology tyanna.stuc...@northsoundderm.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Clia Competency Form
Does anyone have a CLIA competency form for histology supervisor, histotechs, and technical supervisors that they would be willing to share? It would be greatly appreciated!!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vacuolated and torn?
Good morning everyone! One of my pathologists says that we are having a problem with the tissue on the slides looking vacuolated and torn. He is convinced it is from microtomy. Anyone have any ideas? I was thinking that it might be a processing issue. Thanks in advance! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lab related blog post
Hoping all are doing well. Good lab-bad lab blog. http://www.chicagonow.com/downsize-maybe/2016/10/ben-stiller-and-psa-fly-elizabeth-holmes-and-theranos-die/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] KU Size
Dear Histonetters, Searching for a product -an enzyme- its size it is not indicated in volume or weight. It is labelled as 25 KU. Does it mean that the vial contains 25.000 activity units? Thanks Pablo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
