[Histonet] HSV Slides needed for Validation

[Bliven, Laura M via Histonet]

Looking for a commercial or lab source for IHC unstained slides that we can use 
for validation of HSV type I & type II please email me at 
bliven.la...@marshfieldclinic.org.
Thanks,
Laura

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[Histonet] Merkel cell polyomavirus antibody

[Martha Ward-Pathology via Histonet]

Good morning,

I have had a request for this antibody but I am having trouble locating a 
vendor for it.If anyone has any information on vendor, etc. , I would 
appreciate it.   We have Leica Bond 3s.

Thank you in advance for your help.

Martha Ward
Wake Forest Baptist Health

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[Histonet] Tissues storage question

[Blanca Lopez via Histonet]

Dear Histonets,
I would like to ask everybody what do you think about a specific way to storage 
tissues in -80 C for long time of periods and how this type of procedure can 
affect those tissues.
   This is my first time that I heard about this procedure:
I have a customer that storage hundreds of tissues this way, she fixed them in 
4 paraformaldehyde for long time then transfer to a 100% alcohol and storage 
them in -80 C for several years...Can you told me if this procedure is wrong or 
good and how can affect those tissues?
   I told her that I can test a couple to see how they react...I 
process them starting 70% alcohol for 12 hrs. processing, cut them (they were 
little crunchy) and perform an H everything stain pink except the nucleus 
were fade purple. It looks weird to me.

My question is how can she troubleshooting those tissues? Can we fix them in 
formalin again and start a new process? Can we wash the tissues in soap water 
to clean them? How can we do to make those tissue back to normal? Is alcohol a 
bad storage reagent?

I am kind of loss with this case. I really need to understand how the tissue 
get affected to be storage this way. How can I troubleshooting?

Thanks for your advices:)

Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu




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[Histonet] CA 15-3 and CA 27.29

[Roy, Lisa via Histonet]

I was wondering if anyone out there knows of a lab that is performing CA 15-3 
and/or CA 27.29 by IHC on FFPE tissue?  Thanks in advance.

Lisa Roy, HT(ASCP)cm
Histology Supervisor
Worcester, MA

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