Re: [Histonet] Survey!!!!!!

[J B via Histonet]

Stainer for sure.

JB

On Fri, Mar 31, 2017, 2:05 PM Walter Benton via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> ​Patti,
>
>
> If you are able to incorporate the special stains along with the H on
> the automated stainer, I think that would be the best option. Based on your
> information you may have 120-180 slides to coverslip by hand each day.
>
>
> Walter Benton HT(ASCP)QIHC
> Lab Operations Manager
> Chesapeake Urology Associates
> 806 Landmark Drive, Suite 127
> Glen Burnie, MD 21061
> 443-471-5850 (Direct)
> 410-768-5961 (Lab)
> 410-768-5965 (Fax)
> Chesapeakeurology.com
>
> Voted a Best Place to Work by
> Baltimore and Modern Healthcare
> Magazines.
> 
> From: Patti Nelson - PNP Lab Consultant 
> Sent: Friday, March 31, 2017 3:50 PM
> To: Walter Benton; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Survey!!
>
> Walter the staff consist of 1 Histotech and 1 lab assistant. On an average
> there will be 2 special stains per block. Hope that helps.
>
> Sincerely,
>
> PATTI NELSON  H.T.(ASCP)
> PNP LABORATORY CONSULTANTS
> SUPERVISOR DGC/ZADEH LABS
> PO BOX 412
> CABAZON, CA. 92230
> 909-841-9761
> nelsonr...@verizon.net
> CONFIDENTIALITY NOTICE:This message and any included attachments are from
> Patti Nelson, PNP Laboratory Consultants and are intended only for the
> addressee. The information contained in this message is confidential and
> may contain privileged, confidential, proprietary and/or exemption from
> disclosure under applicable law.  Unauthorized forwarding, printing,
> copying, distribution, or use of such information is strictly prohibited
> and may be unlawful.  If you are not the addressee, please promptly delete
> this message and notify the sender ofthe delivery error by e-mail or you
> may call  909-841-9761.
>
>
> -Original Message-
> From: Walter Benton 
> To: Patti Nelson - PNP Lab Consultant ; Histonet <
> histonet@lists.utsouthwestern.edu>
> Sent: Fri, Mar 31, 2017 12:39 pm
> Subject: Re: [Histonet] Survey!!
>
> How many slides are you producing from that number of blocks? How many
> techs do you have that are able to hand stain and/or hand coverslip?
>
> Walter Benton HT(ASCP)QIHC
> Lab Operations Manager
> Chesapeake Urology Associates
> 806 Landmark Drive, Suite 127
> Glen Burnie, MD 21061
> 443-471-5850 (Direct)
> 410-768-5961 (Lab)
> 410-768-5965 (Fax)
> Chesapeakeurology.com
>
> Voted a Best Place to Work by
> Baltimore and Modern Healthcare
> Magazines.
> 
> From: Patti Nelson - PNP Lab Consultant via Histonet <
> histonet@lists.utsouthwestern.edu >>
> Sent: Friday, March 31, 2017 2:58 PM
> To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu>
> Subject: [Histonet] Survey!!
>
> Hi Everyone,
>
> I just wanted to get everyone's opinion. If you had to chose between
> buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you
> chose? Lets say your volume was around 60 to 80 blocks a day and you worked
> for a GI Lab. Everyone's input would be greatly appreciated.
>
>
>
> Sincerely,
>
> PATTI NELSON H.T.(ASCP)
> PNP LABORATORY CONSULTANTS
> SUPERVISOR DGC/ZADEH LABS
> PO BOX 412
> CABAZON, CA. 92230
> 909-841-9761
> nelsonr...@verizon.net
> CONFIDENTIALITY NOTICE:This message and any included attachments are from
> Patti Nelson, PNP Laboratory Consultants and are intended only for the
> addressee. The information contained in this message is confidential and
> may contain privileged, confidential, proprietary and/or exemption from
> disclosure under applicable law. Unauthorized forwarding, printing,
> copying, distribution, or use of such information is strictly prohibited
> and may be unlawful. If you are not the addressee, please promptly delete
> this message and notify the sender ofthe delivery error by e-mail or you
> may call 909-841-9761.
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu >
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> CONFIDENTIALITY NOTICE: The information contained in this electronic
> message is intended solely for the personal and confidential use of the
> designated recipient(s) named above and may contain information that is
> protected from disclosure under applicable law. If you are not the intended
> recipient, or the employee or agent responsible for delivering it to the
> intended recipient, you are hereby notified that any dissemination,
> distribution or copying of this transmission is strictly prohibited. If you
> have received this transmission in error, please notify the transmitting
> person/department immediately by email or telephone (410) 581-5881 and
> delete the message without making a 

Re: [Histonet] Survey!!!!!!

[Walter Benton via Histonet]

​Patti,


If you are able to incorporate the special stains along with the H on the 
automated stainer, I think that would be the best option. Based on your 
information you may have 120-180 slides to coverslip by hand each day.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Patti Nelson - PNP Lab Consultant 
Sent: Friday, March 31, 2017 3:50 PM
To: Walter Benton; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Survey!!

Walter the staff consist of 1 Histotech and 1 lab assistant. On an average 
there will be 2 special stains per block. Hope that helps.

Sincerely,

PATTI NELSON  H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law.  Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful.  If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call  909-841-9761.


-Original Message-
From: Walter Benton 
To: Patti Nelson - PNP Lab Consultant ; Histonet 

Sent: Fri, Mar 31, 2017 12:39 pm
Subject: Re: [Histonet] Survey!!

How many slides are you producing from that number of blocks? How many techs do 
you have that are able to hand stain and/or hand coverslip?

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Patti Nelson - PNP Lab Consultant via Histonet 
>
Sent: Friday, March 31, 2017 2:58 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Survey!!

Hi Everyone,

I just wanted to get everyone's opinion. If you had to chose between buying a 
Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets 
say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. 
Everyone's input would be greatly appreciated.



Sincerely,

PATTI NELSON H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law. Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful. If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call 909-841-9761.

___
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employee or agent responsible for delivering it to the intended recipient, you 
are hereby notified that any dissemination, distribution or copying of this 
transmission is strictly prohibited. If you have received this transmission in 
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Re: [Histonet] Survey!!!!!!

[Patti Nelson - PNP Lab Consultant via Histonet]

Walter the staff consist of 1 Histotech and 1 lab assistant. On an average 
there will be 2 special stains per block. Hope that helps.



Sincerely,

PATTI NELSON  H.T.(ASCP) 
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law.  Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful.  If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call  909-841-9761.




-Original Message-
From: Walter Benton 
To: Patti Nelson - PNP Lab Consultant ; Histonet 

Sent: Fri, Mar 31, 2017 12:39 pm
Subject: Re: [Histonet] Survey!!

How many slides are you producing from that number of blocks? How many techs do 
you have that are able to hand stain and/or hand coverslip?

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Patti Nelson - PNP Lab Consultant via Histonet 

Sent: Friday, March 31, 2017 2:58 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Survey!!

Hi Everyone,

I just wanted to get everyone's opinion. If you had to chose between buying a 
Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets 
say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. 
Everyone's input would be greatly appreciated.



Sincerely,

PATTI NELSON  H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law.  Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful.  If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call  909-841-9761.

___
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intended solely for the personal and confidential use of the designated 
recipient(s) named above and may contain information that is protected from 
disclosure under applicable law. If you are not the intended recipient, or the 
employee or agent responsible for delivering it to the intended recipient, you 
are hereby notified that any dissemination, distribution or copying of this 
transmission is strictly prohibited. If you have received this transmission in 
error, please notify the transmitting person/department immediately by email or 
telephone (410) 581-5881 and delete the message without making a copy.

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Re: [Histonet] Survey!!!!!!

[Walter Benton via Histonet]

How many slides are you producing from that number of blocks? How many techs do 
you have that are able to hand stain and/or hand coverslip?

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: Patti Nelson - PNP Lab Consultant via Histonet 

Sent: Friday, March 31, 2017 2:58 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Survey!!

Hi Everyone,

I just wanted to get everyone's opinion. If you had to chose between buying a 
Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets 
say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. 
Everyone's input would be greatly appreciated.



Sincerely,

PATTI NELSON  H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law.  Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful.  If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call  909-841-9761.

___
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CONFIDENTIALITY NOTICE: The information contained in this electronic message is 
intended solely for the personal and confidential use of the designated 
recipient(s) named above and may contain information that is protected from 
disclosure under applicable law. If you are not the intended recipient, or the 
employee or agent responsible for delivering it to the intended recipient, you 
are hereby notified that any dissemination, distribution or copying of this 
transmission is strictly prohibited. If you have received this transmission in 
error, please notify the transmitting person/department immediately by email or 
telephone (410) 581-5881 and delete the message without making a copy.

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Re: [Histonet] Auto Stainer vs Auto Coverslipper

[Mequita Praet via Histonet]

Hands down the Automatic Stainer preferred over the coverslipper. You can
do H & E  & you can do the AB/PAS as well on it. No standing there hand
dipping. You can be doing something else and come back and take them off to
sit and hand coverslipp. Email me personally if you want to know which
Automatic Stainer I have many years of experience with. It is a work horse.
With yearly maintenance hardly ever breaks down.
Mequita Praet, HTL(ASCP)SLS
mdpr...@gmail.com
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Re: [Histonet] [EXTERNAL] Re: Tissue fixation

[Elizabeth Chlipala via Histonet]

I'm going to give my two cents here.  I think you need to look closely at how 
you handle these samples.  First of all leeps are not small samples therefore 
just because the leep has been placed in fixative upon removal and then sits in 
fixative until its processed.  Unless the sample is grossed  into smaller 
pieces only then it will adequately fix.   Electro cautery  instruments 
function to do two things, they cut and then cauterize - there is always a 
thermal defect associated with the process or thermal spread, this is clearly 
evident on the H slides.   The process is essentially coagulating the tissue 
proteins.  The thermal spread and char that is associated with this process 
varies dependent upon the instrument and setting that is being used.  This 
process may decrease fixative penetration so it's important that these samples 
are grossed in as soon as possible.  Trimming the tissue will allow for better 
penetration of the fixative and good fixation requires at least 4 to 6 hour
 s in formalin AFTER the tissue has been trimmed in.The artifact that the 
pathologist is seeing might not be related to the amount of time the sample 
sits in formalin it might be due to the amount of time the samples sits  in 
formalin prior to being grossed in.

If I had this issue I would start tracking the different times associated with 
these samples and possibly the instrument and setting used (if you are able to 
get that info).   

1.  Time to fixative - cold ischemic time
2.  Time to when the sample is grossed in 
3.  Time in formalin once the sample is grossed in

Good Luck I hope this helps.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Stedman, Nancy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 31, 2017 12:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] [EXTERNAL] Re: Tissue fixation

I would bet there are quite a few pathologists on this mailing list (like me) 
who are here because we respect histo techs and know you have valuable 
information to offer!

I agree, cautery artifact is pretty easy to tell from poor fixation, so it 
should be easy to determine if that is the problem.  Sometimes, specimens that 
are submitted smashed in a cassette may have poor fixation of the tissue in 
contact with the cassette.  Or if they are submitted sponged and floating, some 
tissue may not be fully immersed in formalin.  Also, crush artifact may 
resemble poor fixation, so maybe the clinical team is not handling the tissue 
gently enough?  

I read veterinary specimens; we don't do LEEPs so I don't know if any of these 
scenarios are possible, but they are things I see frequently.  

-Nancy Stedman


-Original Message-
From: Mayer,Toysha N via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 31, 2017 2:15 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL] Re: [Histonet] Tissue fixation

I agree with Rene.  To discredit the pathologist theory  show if all of the 
specimens from the run are not fixed properly.  Then show if it is just the 
LEEPs.  Then show if it is that particular clients specimens?  Then onto that 
client's LEEPs.
That should prove your problem lies with the client handling and not the 
fixation on your end.  Cauterization can cause burning of the specimen, but not 
look unfixed. If it was left out of formalin, autolysis can set in.
The situation shows the god-complex some physicians have and the lack of 
respect they have for their techs.  
This is why we should all be certified and keep up with continuing education, 
so that these pathologists will respect us.
Ok, I'm going to get off my soapbox now, before I say something ugly.

Toysha Mayer






--

Message: 3
Date: Fri, 31 Mar 2017 12:50:37 +
From: T H 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Tissue Fixation
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or 

[Histonet] Survey!!!!!!

[Patti Nelson - PNP Lab Consultant via Histonet]

Hi Everyone,

I just wanted to get everyone's opinion. If you had to chose between buying a 
Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets 
say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. 
Everyone's input would be greatly appreciated. 



Sincerely,

PATTI NELSON  H.T.(ASCP) 
PNP LABORATORY CONSULTANTS
SUPERVISOR DGC/ZADEH LABS
PO BOX 412
CABAZON, CA. 92230
909-841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti 
Nelson, PNP Laboratory Consultants and are intended only for the addressee. The 
information contained in this message is confidential and may contain 
privileged, confidential, proprietary and/or exemption from disclosure under 
applicable law.  Unauthorized forwarding, printing, copying, distribution, or 
use of such information is strictly prohibited and may be unlawful.  If you are 
not the addressee, please promptly delete this message and notify the sender 
ofthe delivery error by e-mail or you may call  909-841-9761.

___
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Histonet@lists.utsouthwestern.edu
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Re: [Histonet] [EXTERNAL] Re: Tissue fixation

[Stedman, Nancy via Histonet]

I would bet there are quite a few pathologists on this mailing list (like me) 
who are here because we respect histo techs and know you have valuable 
information to offer!

I agree, cautery artifact is pretty easy to tell from poor fixation, so it 
should be easy to determine if that is the problem.  Sometimes, specimens that 
are submitted smashed in a cassette may have poor fixation of the tissue in 
contact with the cassette.  Or if they are submitted sponged and floating, some 
tissue may not be fully immersed in formalin.  Also, crush artifact may 
resemble poor fixation, so maybe the clinical team is not handling the tissue 
gently enough?  

I read veterinary specimens; we don't do LEEPs so I don't know if any of these 
scenarios are possible, but they are things I see frequently.  

-Nancy Stedman


-Original Message-
From: Mayer,Toysha N via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 31, 2017 2:15 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL] Re: [Histonet] Tissue fixation

I agree with Rene.  To discredit the pathologist theory  show if all of the 
specimens from the run are not fixed properly.  Then show if it is just the 
LEEPs.  Then show if it is that particular clients specimens?  Then onto that 
client's LEEPs.
That should prove your problem lies with the client handling and not the 
fixation on your end.  Cauterization can cause burning of the specimen, but not 
look unfixed. If it was left out of formalin, autolysis can set in.
The situation shows the god-complex some physicians have and the lack of 
respect they have for their techs.  
This is why we should all be certified and keep up with continuing education, 
so that these pathologists will respect us.
Ok, I'm going to get off my soapbox now, before I say something ugly.

Toysha Mayer






--

Message: 3
Date: Fri, 31 Mar 2017 12:50:37 +
From: T H 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Tissue Fixation
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim



--

Message: 4
Date: Fri, 31 Mar 2017 13:32:35 + (UTC)
From: Rene J Buesa 
To: T H ,   "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Tissue Fixation
Message-ID: <1730848134.8065268.1490967155...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

What you describe as a possible scenario is absolutely possible.If your PT does 
not "want to hear" about it, suggest she gets a "hearing aid" or to study 
something about histotechnology or even better yet, pay attention to what a 
professional on the subject (you) has to say about it. You would never dare to 
question her diagnosis, why would she question yours on this subject?Ren? 

On Friday, March 31, 2017 9:11 AM, T H via Histonet 
 wrote:
 

 Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.? She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).? She claims we must be diluting our formalin to cause this 
issue or "something".? We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some 

Re: [Histonet] Tissue fixation

[Bob Richmond via Histonet]

Tim describes a problem "I have a pathologist that is not happy with the
fixation on some of our LEEP specimens."

LEEP specimens are inherently crappy, because of the cautery used to obtain
them, and the resulting cautery artifact in the specimens. In the last few
years they've turned the voltage down, and that's helped some - and perhaps
this particular clinic hasn't turned the voltage down.

I wonder how familiar your pathologist is with the whole process - the sort
of homely detail not taught in pathology residency, and the sort of detail
I often go out and research on my own. It's helpful to all to know that the
clinical stakes here are not high - we do report margins on cervical LEEP
specimens (when we can figure them out), but clinical outcomes apparently
don't have much to do with whether margins are positive for dysplasia or
not.

It's impossible to get india ink to stick to the cauterized gross margins,
but the cautery artifact is painfully obvious under the microscope anyway.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: [Histonet] Tissue fixation

[Mayer,Toysha N via Histonet]

I agree with Rene.  To discredit the pathologist theory  show if all of the 
specimens from the run are not fixed properly.  Then show if it is just the 
LEEPs.  Then show if it is that particular clients specimens?  Then onto that 
client's LEEPs.
That should prove your problem lies with the client handling and not the 
fixation on your end.  Cauterization can cause burning of the specimen, but not 
look unfixed. If it was left out of formalin, autolysis can set in.
The situation shows the god-complex some physicians have and the lack of 
respect they have for their techs.  
This is why we should all be certified and keep up with continuing education, 
so that these pathologists will respect us.
Ok, I'm going to get off my soapbox now, before I say something ugly.

Toysha Mayer






--

Message: 3
Date: Fri, 31 Mar 2017 12:50:37 +
From: T H 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Tissue Fixation
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim



--

Message: 4
Date: Fri, 31 Mar 2017 13:32:35 + (UTC)
From: Rene J Buesa 
To: T H ,   "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Tissue Fixation
Message-ID: <1730848134.8065268.1490967155...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

What you describe as a possible scenario is absolutely possible.If your PT does 
not "want to hear" about it, suggest she gets a "hearing aid" or to study 
something about histotechnology or even better yet, pay attention to what a 
professional on the subject (you) has to say about it. You would never dare to 
question her diagnosis, why would she question yours on this subject?Ren? 

On Friday, March 31, 2017 9:11 AM, T H via Histonet 
 wrote:
 

 Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.? She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).? She claims we must be diluting our formalin to cause this 
issue or "something".? We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.? Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.? That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?? Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim

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Re: [Histonet] disposable grossing pad

[Mayer,Toysha N via Histonet]

We use chux here as well.  The cotton in them can bunch up, also they don't lay 
completely flat.  We also use the thin lab mats.  Cardinal carries them as does 
fisher.  They can be bought in bulk, have an absorbent side and a fluid 
resistant side.  Since we are on a tight budget here, and students are 
wasteful, they work well for us.  A pack of 30 or so lasts us about two 
calendar months.  We use 1/day and 3/week.
There is also a bench top liner that you can purchase.  It may come in a roll, 
and you can just cut what you need.
As for disposal, since they are contaminated with specimens, we place ours 
directly in the biohazard.  Any item that may have come in contact with an 
unprocessed specimen (no blades or glass) goes into the biohazard waste.  
Hope this helps.

Toysha Mayer

Message: 1
Date: Thu, 30 Mar 2017 17:42:55 +
From: "Terri  Braud" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: Re: [Histonet] disposable grossing pad
Message-ID:
<48E053DDF6CE074DB6A7414BA05403F812D064@HRHEX03-HOS.holyredeemer.local>

Content-Type: text/plain; charset="us-ascii"

I know many, including staff here, that prefer to use "chux".  They are very 
cheap, come in various sizes and adsorption levels, latex-free, with a highly 
absorbent core and soft, quick-drying coverstock so you don't get lint in your 
specimens. Use your favorite search engine to look them up.  Almost all major 
Med suppliers carry them.  Between specimens, we just toss the old one into 
red-bag waste.
I hope this helps.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

   1. formalin pad for grossing (Wang, Weixi)
--
Message: 1
Date: Wed, 29 Mar 2017 17:09:25 +
From: "Wang, Weixi" 
Subject: [Histonet] formalin pad for grossing Dear all Histonetters, It has 
been a while since I joined the mailing list and I learned a lot here!
Now I have a question and would like to get feedbacks regarding the use of 
formalin in the grossing room. I was instructed to use the formalin-neutralized 
pads during the grossing. Any other pads or paper, absorbent or not, cannot go 
to the bio-hazardous waste. But the neutralizer pads are so costly, especially 
for grossing part, when we need to change very frequently to void the cross 
contamination between specimen.
I would like to know what is the pad/mat/paper you use for grossing, and how do 
you dispose these formalin contaminated materials.
Any feedback is deeply appreciated!
Weixi
Weixi Wang, Ph.D., HTL(ASCP)
Manager | Histology Laboratory
St. Joseph's Healthcare System
703 Main Street, Paterson, NJ 07503
Phone: 973.754.3541 | Fax: 973.754.3292
wangwe...@sjhmc.org | 
www.StJosephsHealth.org
***








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Re: [Histonet] Histonet Digest, Vol 160, Issue 30 Subject: disposable grossing pad for specimen imaging

[Steve McClain via Histonet]

We use squares of absorbent paper towels to first blot excess fixative and then 
place on a 2mm thickness piece of art foam.
Art foam is used as a background for 
photography and slicing.
By sliding or moving the foam we position the specimen under the camera during 
specimen photography. We have photographed every specimen since 2004. 
Send me an email and I will reply w images to illustrate our method.
Steve A. McClain, MD

> On Mar 31, 2017, at 13:25, "histonet-requ...@lists.utsouthwestern.edu" 
>  wrote:
> 
> Subject: Re: [Histonet] disposable grossing pad

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Re: [Histonet] Tissue Fixation

[Gudrun Lang via Histonet]

I second the opinion of Joyce. We see such effects in portio-conisations, that 
are done with a thermo-electrical knife. The surface and the underlying area 
show a very pink colour in HE. It can also be seen in prostata-chips. IHC on 
such biopsies shows the effect of an non-stainable edge with a clear cut 
between positive and negative. 
https://www.uni-marburg.de/fb20/zahnerhaltk/lehre/Download/Bilder/35_plattenepithelmetaplasie_portio_02212427.jpg
I've found this picture, that shows a typical conisation-section.
regards
Gudrun 

-Ursprüngliche Nachricht-
Von: Weems, Joyce K. via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Freitag, 31. März 2017 17:26
An: Morken, Timothy; Rene J Buesa; T H; Histonet@lists.utsouthwestern.edu
Betreff: Re: [Histonet] Tissue Fixation

Aren't LEEPS done with some sort of electric method that will damage the tissue 
before it even reaches formalin. I'm not positive but Google it - I believe 
that might be the problem. j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
770-380-8099 Cell
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph’s 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, March 31, 2017 11:07 AM
To: Rene J Buesa ; T H 
Cc: Histonet 
Subject: Re: [Histonet] Tissue Fixation

Tim, I have to agree with Rene that the formalin or time in formalin is 
obviously not the problem - it has plenty of time in formalin (and who would 
dilute it anyway?). Handling before formalin must always be determined when 
problems arise. If the sample sits on a paper towel, gauze etc it does not 
really degrade faster, rather the tissue may dry out and so fixes by 
dehydration rather than by formalin. It may be the formalin cannot get into the 
tissue in those dried out portions of the tissue. However that is just 
speculation. Since these all seem to be from one particular client, the client 
is the place to start. The only way to determine the problem is to follow the 
specimen from start to finish. Can you or someone you trust physically observe 
the way samples are handled from the time they are taken to the time put in 
formalin? One issue I always run up against is people  saying they do one thing 
but actually doing another. And they may realize during questioning that they 
are not doing it right but don't want to admit it. It wastes a lot of time. 
I've had physicians tell me to ignore the part of the process they are 
responsible for because they do it right. I just tell them that to be complete 
we need to follow the process from start to finish, nothing personal, just 
business. Leaving any part out may lead to not resolving the problem. Probably 
99% of the process is just fine, but that 1% is damaging the sample and needs 
to be illuminated.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center



-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, March 31, 2017 6:33 AM
To: T H; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue Fixation

What you describe as a possible scenario is absolutely possible.If your PT does 
not "want to hear" about it, suggest she gets a "hearing aid" or to study 
something about histotechnology or even better yet, pay attention to what a 
professional on the subject (you) has to say about it. You would never dare to 
question her diagnosis, why would she question yours on this subject?René

On Friday, March 31, 2017 9:11 AM, T H via Histonet 
 wrote:


 Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits 

[Histonet] Disposal of slides/blocks

[Lester Raff MD via Histonet]

Our lab is once again evaluating disposal of slides/blocks from >10 years ago. 
Maintaining or donating the material do not appear to be feasible options.

Aside from PHI issues, how are labs disposing of this type of material. Do you 
place the slides in sharps containers?  Do you red bag the blocks?

Thanks.

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] Update on Trimming soft brain

[Julio Benavides via Histonet]

Hi there,


just to update you on the issue. Thanks to the kind and useful 
suggestions from histonetes, I found the solution to my problem.


Fixation for two days in 10% formalin in absolute ethanol worked 
perfectly well. Brains were well fixed, we could trimmed them, and the 
fixation in alcoholic formalin did not interfere with the IHCs, at least 
with the one we are using (CD3, bAPP and GFAP).


Again, thank you so much for your help!

cheers

Julio


El 06/03/2017 a las 23:18, Julio Benavides via Histonet escribió:

Hi there,

I´m trying to trim ovine foetal brain (90 days of gestation). The 
brain is
too soft and there is no way to get a proper slice. It has been in 
buffered

10% formalin for three weeks but still too soft. When you take the brain
out of the pot and put into the trimming table, it just kind of spill 
over
the place. We have tried to embed the brain in 7.5% gelatin, which 
helps to
keep the shape of the brain before trimming, but as soon you cut the 
slice,

the centre of the tissue just melt away. I was wondering if there is any
post fixative solution we can try. May be Bouin´s?

Any suggestion would be greatly appreciated!

Cheers

Julio
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[Histonet] Tissue banking

[Judi Ford via Histonet]

Hi everyone, Happy Friday to you all..

I am hoping to gather some information on how people bank frozen tissue for 
work in research. I am trying to start a bank of frozen human samples stored in 
a -80 freezer for our scientists use.  Information that I'm looking for is as 
follows:

1.   Accessioning software/database - what software do you use to keep 
track of samples and their useage. What information is useful for you in 
keeping track of the samples.

2.   Storing samples in the freezer - how do you store them; wrapped foil, 
in cryoboxes, in ziplock baggies, etc.

3.   Do you have a system of checking out the samples? If so, how do you 
keep track and control of the samples?

4.   Do you have one person who is in charge of the tissue bank? If so what 
are their responsibilities?

Thanks for your help with this.  If you want to send me information offline 
here is my email address: jf...@cytomx.com.

Thank you and I hope you have a really great weekend.
Judi Ford

SRA
CytomX Therapeutics

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Re: [Histonet] Tissue Fixation

[Weems, Joyce K. via Histonet]

Aren't LEEPS done with some sort of electric method that will damage the tissue 
before it even reaches formalin. I'm not positive but Google it - I believe 
that might be the problem. j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
770-380-8099 Cell
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph’s 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, March 31, 2017 11:07 AM
To: Rene J Buesa ; T H 
Cc: Histonet 
Subject: Re: [Histonet] Tissue Fixation

Tim, I have to agree with Rene that the formalin or time in formalin is 
obviously not the problem - it has plenty of time in formalin (and who would 
dilute it anyway?). Handling before formalin must always be determined when 
problems arise. If the sample sits on a paper towel, gauze etc it does not 
really degrade faster, rather the tissue may dry out and so fixes by 
dehydration rather than by formalin. It may be the formalin cannot get into the 
tissue in those dried out portions of the tissue. However that is just 
speculation. Since these all seem to be from one particular client, the client 
is the place to start. The only way to determine the problem is to follow the 
specimen from start to finish. Can you or someone you trust physically observe 
the way samples are handled from the time they are taken to the time put in 
formalin? One issue I always run up against is people  saying they do one thing 
but actually doing another. And they may realize during questioning that they 
are not doing it right but don't want to admit it. It wastes a lot of time. 
I've had physicians tell me to ignore the part of the process they are 
responsible for because they do it right. I just tell them that to be complete 
we need to follow the process from start to finish, nothing personal, just 
business. Leaving any part out may lead to not resolving the problem. Probably 
99% of the process is just fine, but that 1% is damaging the sample and needs 
to be illuminated.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center



-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, March 31, 2017 6:33 AM
To: T H; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue Fixation

What you describe as a possible scenario is absolutely possible.If your PT does 
not "want to hear" about it, suggest she gets a "hearing aid" or to study 
something about histotechnology or even better yet, pay attention to what a 
professional on the subject (you) has to say about it. You would never dare to 
question her diagnosis, why would she question yours on this subject?René

On Friday, March 31, 2017 9:11 AM, T H via Histonet 
 wrote:


 Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim

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Re: [Histonet] Tissue Fixation

[Morken, Timothy via Histonet]

Tim, I have to agree with Rene that the formalin or time in formalin is 
obviously not the problem - it has plenty of time in formalin (and who would 
dilute it anyway?). Handling before formalin must always be determined when 
problems arise. If the sample sits on a paper towel, gauze etc it does not 
really degrade faster, rather the tissue may dry out and so fixes by 
dehydration rather than by formalin. It may be the formalin cannot get into the 
tissue in those dried out portions of the tissue. However that is just 
speculation. Since these all seem to be from one particular client, the client 
is the place to start. The only way to determine the problem is to follow the 
specimen from start to finish. Can you or someone you trust physically observe 
the way samples are handled from the time they are taken to the time put in 
formalin? One issue I always run up against is people  saying they do one thing 
but actually doing another. And they may realize during questioning that they 
are not doing it right but don't want to admit it. It wastes a lot of time. 
I've had physicians tell me to ignore the part of the process they are 
responsible for because they do it right. I just tell them that to be complete 
we need to follow the process from start to finish, nothing personal, just 
business. Leaving any part out may lead to not resolving the problem. Probably 
99% of the process is just fine, but that 1% is damaging the sample and needs 
to be illuminated.


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 31, 2017 6:33 AM
To: T H; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue Fixation

What you describe as a possible scenario is absolutely possible.If your PT does 
not "want to hear" about it, suggest she gets a "hearing aid" or to study 
something about histotechnology or even better yet, pay attention to what a 
professional on the subject (you) has to say about it. You would never dare to 
question her diagnosis, why would she question yours on this subject?René 

On Friday, March 31, 2017 9:11 AM, T H via Histonet 
 wrote:
 

 Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim

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Re: [Histonet] Tissue Fixation

[Paula Keene Pierce via Histonet]

Bravo Rene! Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, 
Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com

  From: Rene J Buesa via Histonet 
 To: T H ; "histonet@lists.utsouthwestern.edu" 
 
 Sent: Friday, March 31, 2017 8:44 AM
 Subject: Re: [Histonet] Tissue Fixation
   
What you describe as a possible scenario is absolutely possible.If your PT does 
not "want to hear" about it, suggest she gets a "hearing aid" or to study 
something about histotechnology or even better yet, pay attention to what a 
professional on the subject (you) has to say about it. You would never dare to 
question her diagnosis, why would she question yours on this subject?René 

    On Friday, March 31, 2017 9:11 AM, T H via Histonet 
 wrote:
 

 Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim

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Re: [Histonet] Tissue Fixation

[Rene J Buesa via Histonet]

What you describe as a possible scenario is absolutely possible.If your PT does 
not "want to hear" about it, suggest she gets a "hearing aid" or to study 
something about histotechnology or even better yet, pay attention to what a 
professional on the subject (you) has to say about it. You would never dare to 
question her diagnosis, why would she question yours on this subject?René 

On Friday, March 31, 2017 9:11 AM, T H via Histonet 
 wrote:
 

 Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim

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[Histonet] Tissue Fixation

[T H via Histonet]

Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim

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