Re: [Histonet] Congo Red
Carl Hobbs FIBMS, Histology and Imaging Manager at Kings College in London replies: >>That's a good point, Bob (mouse amyloid controls for human sections). The amyloid produced by the the various mouse models (TASTPM, TGs) is human amyloid... as you know. I suspect that it wouldn't be accepted as a positive control (I don't know) because it isn't pathologically produced. It is genetically induced as you know).<< There are more then ten structurally unrelated proteins capable of arranging into clinically significant amyloid in people. They have slightly different tinctorial properties, and of course react differently to immunohistochemicaly studies. I don't know of any regulatory agency that has rules about amyloid controls. The mouse models of amyloidosis involve injection of casein (or diazotized casein). I haven't read anything about these for close to fifty years, and there may be better methods now. I'd want an animal amyloid that was either AA or AL type amyloid. Bob Richmond Samurai Pathologist Maryville, Tennessee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Congo Red- Glass wool
Fran, I've never heard of that. The books I looked at just say "filter" with no specific filter recommendations. For Sudan Black and Congo red we use Whatman "Reeve Angel 802" (Pleated). It has a 15um pore size which is "coarse." Fisher catalog number 09-901A Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Frances Pearsall via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, March 26, 2018 12:28 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Congo Red- Glass wool Can anyone tell me what kind and/or size glass wool do you use to for filtering the Working Congo Red solution right before use? From what I've read, it recommends not to use regular filter paper. Thank you so much, Fran Pearsall, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Congo Red- Glass wool
Can anyone tell me what kind and/or size glass wool do you use to for filtering the Working Congo Red solution right before use? From what I've read, it recommends not to use regular filter paper. Thank you so much, Fran Pearsall, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PRN opening
New England dermatology in Springfield, MA has an immediate opening for an experienced, per-diem HT in our dermatopathology and Mohs department . Responsibilities will include embedding, microtomy and staining. Mohs experience is a plus. FT potential. Contact: Terry Molnar, Human Resources New England dermatology and Laser Center 3455 Main St Springfield, MA 01107 (413)733-9600 x 319 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Silver waste
Hi All. Happy Monday! Would anyone share how they collect, store, and dispose of silver waste from manual special stains such as GMS, Melanin Fontana-Masson, and Calcium Von Kossa? Thanks in advance! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
