[Histonet] counter stains for Sudan Black B

2018-11-08 Thread Tyrone Genade via Histonet
Hello, I need a counterstain for use together with Sudan Black B. I am using this method: https://www.ncbi.nlm.nih.gov/pubmed/27812872 which uses Nuclear Fast Red. I don't have nuclear fast red... In an updated protocol by these authors they use hematoxylin but in a previous trial I found it was

[Histonet] Gout specimens

2018-11-08 Thread Anne Murvosh via Histonet
I haven't done a gout specimen in years, I remember the processing part, not remembering the Staining part. Could someone please send me your gout protocol? Thanks Anne Murvosh HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Freezing artefact

2018-11-08 Thread Finlay Finlay via Histonet
Good Afternoon all, I have a couple of cases where my post mortem samples have been accidentally frozen in Neutral Buffered Formalin. Naturally this has led to pretty striking artefact in the tissue. I was wondering if anyone who perhaps has more experience of dealing with frozen tissue has