[Histonet] Florida Cytology

2019-09-05 Thread Ashwin Ashwin via Histonet
Hello,
Do we need to have a licensed Histotech to perform pre-analytical non-gyn 
Cytology slide prep? Kindly advise 
Thanks

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[Histonet] Tissue Processor Validation

2019-09-05 Thread Ashwin Ashwin via Histonet
Hello Everyone,
If we change a processing protocol and are in need to recalibrate our tissue 
processors, is it required to perform concordance study between the old 
protocol and the new?
Please let me know
Thanks

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[Histonet] Leica Bond Max - non OEM products?

2019-09-05 Thread Dr. Stephen Chang [UML] via Histonet
Hello,

 

I was referred to join this listserv due to a question we have about our
Leica Bond Max. I work in a medical diagnostic laboratory. Does anyone know
where we can get good quality but cheaper reagents to use on the Leica Bond
Max, perhaps from a third party? The prices from Leica directly are somewhat
pricey and the person who referred me said she believes that many labs use
"aftermarket" supplies. I'm talking about epitope retrieval and wash
solution, etc, not antibodies.

 

Thanks in advance!

 

Sincerely,

 

Stephen W. Chang, MD, MBA


United Medical Laboratories
1980 Gallows Rd, Ste 300
Tysons, VA 22182
703-356-4422 (phone)
703-356-2460 (fax)
www.UnitedMedicalLab.com


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Re: [Histonet] How do you section PPFE tissue in embedding rings?

2019-09-05 Thread Paula Keene Pierce via Histonet
Hi,
I used embedding rings back in the 80s and still use the chuck adapter for 
large blocks. I do eyes.
I have a couple John's Hopkins chuck adapters and could loan you one.

Send me a picture of your microtome with a closeup of the chuck adapter to see 
if it will fit.
Do you have an old AO 820 sitting around? I know it will fit it.
The old Leica 1512s had an adapter that would work too.
Otherwise, I suggest just re-embedding.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, September 5, 2019, 01:16:21 PM CDT, Preiszner, Johanna via 
Histonet  wrote:  
 
 Hi,

I received a lot of specimens embedded in plastic embedding rings...have a 
Leica RM2135 microtome.

How can I put these blocks in the clamps of the microtome?

Searched Internet with zero success.

Nobody in town ever used embedding rings so I can not borrrow an adapter if 
that's what I need.

Could anybody please help out?

Re-embedding the tissue in regular cassettes would cost the client a lot of 
money.


Thanks,

Johanna Preiszner
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Re: [Histonet] Cryostat cooling time

2019-09-05 Thread Lynn Whitmore via Histonet
The amount of time a cryostat takes to get to cutting temperature depends on 
the age of the machine, manufacturer, and air flow around the cryostat. We have 
4 Avantiks the newer ones cool faster than the older ones and the one that is 
under a wall cabinet with about 2 feet from the top of the cryostat to the 
bottom of the cabinet takes about an hour longer to cool.  The cryostats are 
kept at -27. The cooling time for the quicker one is 2 hours from room 
temperature (70 degrees Fahrenheit) and the slower one is 3 hours.

I would suggest you plan for 4 hours and monitor the cooling time so you know 
for the next time you need to shutdown the cryostat. I have had Leicas and 
Thermo Fisher cryostats and they were different. Hope this helps.



-Original Message-
>From: histonet-requ...@lists.utsouthwestern.edu
>Sent: Sep 5, 2019 1:00 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: Histonet Digest, Vol 190, Issue 3
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>Today's Topics:
>
>   1. Re: CPT Code 88172 (Webster, Thomas S.)
>   2. Re: CPT Code 88172 (Joe W. Walker, Jr.)
>   3. Re: CPT Code 88172 (Victoria Baker)
>   4. PPE for frozen sections (Moe, Barbi A)
>   5. DAKO Artisan special stain kit validations (Moe, Barbi A)
>   6. Cryostat to room temperature (Moe, Barbi A)
>
>
>--
>
>Message: 1
>Date: Wed, 4 Sep 2019 17:31:03 +
>From: "Webster, Thomas S." 
>To: "'histonet@lists.utsouthwestern.edu'"
>   
>Subject: Re: [Histonet] CPT Code 88172
>Message-ID: 
>Content-Type: text/plain; charset="us-ascii"
>
>You can only bill for 88172 if the pathologist looks at the FNA slide(s) 
>during the procedure.  Otherwise do not attempt to bill for it.  It is a 
>physician code.
>
>
>CONFIDENTIALITY NOTICE:
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>Message: 2
>Date: Wed, 4 Sep 2019 18:07:26 +
>From: "Joe W. Walker, Jr." 
>To: "Webster, Thomas S." 
>Cc: "histonet@lists.utsouthwestern.edu"
>   
>Subject: Re: [Histonet] CPT Code 88172
>Message-ID:
>   
> 
>   
>Content-Type: text/plain; charset="utf-8"
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>Hi Thomas,
>
>What is your reference source for stating this is "a physician code"?  In 
>reviewing our coding data, this code carries a facilities component in 
>addition to a professional component.  I agree that a pathologist must perform 
>the review in order to bill for both, but I am aware of people in New England 
>who bill just the TC when a technologist provides the adequacy interpretation 
>and some payers cover this charge.
>
>Joe W. Walker, Jr. MS, SCT(ASCP)
>Anatomical Pathology Manager
>joewal...@rrmc.org, www.rrmc.org
>
>-Original Message-
>From: Webster, Thomas S. via Histonet 
>Sent: Wednesday, September 4, 2019 1:31 PM
>To: 'histonet@lists.utsouthwestern.edu' 
>Subject: Re: [Histonet] CPT Code 88172
>
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>You can only bill for 88172 if the pathologist looks at the FNA slide(s) 
>during the procedure.  Otherwise do not attempt to bill for it.  It is a 
>physician code.
>
>
>CONFIDENTIALITY NOTICE:
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[Histonet] How do you section PPFE tissue in embedding rings?

2019-09-05 Thread Preiszner, Johanna via Histonet
Hi,

I received a lot of specimens embedded in plastic embedding rings...have a 
Leica RM2135 microtome.

How can I put these blocks in the clamps of the microtome?

Searched Internet with zero success.

Nobody in town ever used embedding rings so I can not borrrow an adapter if 
that's what I need.

Could anybody please help out?

Re-embedding the tissue in regular cassettes would cost the client a lot of 
money.


Thanks,

Johanna Preiszner
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