Re: [Histonet] GSH Virtual Summit- THIS SATURDAY

[Colleen Forster via Histonet]

I'd love to join you but I will be working that entire day

Colleen Forster

On Wed, May 19, 2021 at 3:24 PM Lauren Sweeney via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Everyone,
>
> It is not too late to join us for the GSH Virtual Summit on May 22, 2021
> beginning at 8:00 am until 4:30 pm.
> This virtual meeting will be a great opportunity to get 6 CEU’s through
> some excellent speakers/sessions and we hope to do another in the fall in
> order to stay committed to providing the needed education of histotechs.
> Please visit our website at
> https://georgiahistotech.org/gsh-spring-symposium/ for the registration
> form. Cost is $10.00 per session or $50.00 for all 6 sessions. You can do
> 1, 2 or more sessions for the day.
> Hope to see you there,
> Ely Klar
> GSH President
>
>
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>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
612-626-1930
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[Histonet] GSH Virtual Summit- THIS SATURDAY

[Lauren Sweeney via Histonet]

Hi Everyone,

It is not too late to join us for the GSH Virtual Summit on May 22, 2021 
beginning at 8:00 am until 4:30 pm.
This virtual meeting will be a great opportunity to get 6 CEU’s through some 
excellent speakers/sessions and we hope to do another in the fall in order to 
stay committed to providing the needed education of histotechs.
Please visit our website at https://georgiahistotech.org/gsh-spring-symposium/ 
for the registration form. Cost is $10.00 per session or $50.00 for all 6 
sessions. You can do 1, 2 or more sessions for the day.
Hope to see you there,
Ely Klar
GSH President


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Re: [Histonet] Movats

[Mayer,Toysha N via Histonet]

Hey Betsy,

It could also be the woodstain scarlet and the saffron.  I would omit the 5% 
acetic after the phosphotungstic. 
Take care,
Toysha Mayer

Message: 1
Date: Fri, 7 May 2021 22:11:30 +
From: John Kiernan 
To: "histonet@lists.utsouthwestern.edu"
,Betsy Molinari

Subject: Re: [Histonet] Movats
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

Dear Betsy,

Don't say you are sorry for putting a long post on Histonet! To get 
troubleshooting help you need to say exactly what you did. If you wrote only, 
"why are my sections brown after Movat staining", nobody would understand your 
problem.

Your procedure starts with an hour in hot Bouin. For many years this has been a 
routine prior to trichrome stains done on sections of specimens fixed in 
neutral formaldehyde. It isn't part of Movat's original method (Arch. Path. 
60:209-295, 1955), which probably was devised for sections optimally fixed for 
trichrome staining (in mixtures containing mercuric chloride).

Movat's pentachrome is a trichrome method preceded by alcian blue (for no 
obvious reason) and an iron-haematoxylin for nuclei and elastin. It differs 
from older trichromes in using a mixture of yellow polyene dyes  (saffron) to 
stain the collagen, instead of the blues or greens as in the Mallory and Masson 
methods.

Your method includes "5% sodium thiosulfate -1 min" after the iron-haematoxylin 
stain for black nuclei and elastic fibres. This also isn't part of Movat's 
pentachrome method, and I wonder why. Did you inherit an informal list of 
instructions passed on within the lab?  After a mercuric fixative, hydrated 
sections are dipped in iodine, followed by thiosulphate, before staining, to 
remove a black deposit (probably mercurous chloride) introduced by the 
fixative.I've been seeing similar informal passing of bad staining instructions 
in research labs for many years.  Are you a victim of this trend?

The thiosulphate step in your procedure obviously does no harm, because you got 
the right results with the dog tissues. There may be something different about 
your human specimens: perhaps inadequate fixation, or excessive acid treatment 
(if that's what Cal rite is) for decalcification.

If the sections of human arteries look OK with a microscope, it might not 
matter that grossly they are a different colour from the dog small intestine 
sections. They are, after all, different tissues.

A rather long, and not very helpful reply!

John Kiernan
London, Canada
= = =

From: Betsy Molinari via Histonet 
Sent: May 5, 2021 9:46 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Movats

Hi Histonetters,
I have received several human vessels for paraffin processing and to stain the 
sections for H and Movats. The H were fine. The human sections turned 
brownish yellow with the Movats.The control which is canine small intestine was 
perfect.
The protocol is standard
Bouins 1hr in 58C waterbath
Rinse till yellow disappears
Rinse in DH2O
1% Alcian Blue -20 min
Rinse in running tap H2O -5min
Alkaline alcohol-1hr
Rinse 10 min tap H2O
Rinse in DH2O
Verhoff's Hematoxylin -15 min
3 changes DH2O
Differentiate in 2% FeCl
Rinse in DH2O
5% sodium Thiosulfate -1min
Rinse in running tap-10 min
Rinse in DH2O
Woodstain scarlet/acid fuchsin-1.5 min
Rinse in DH2O
Rinse in 0.5% acetic acid water
5% aqueous phosphotungstic acid -2 changes 5 min each Rinse in 5% acetic acid 
water Rinse in 3 changes absolute ETOH 6% alcoholic  Safran solution Absolute 
alcohol-xylene-coverslip The human slides were fine until the Safran step. When 
I removed them from the stain into the 100% they were a yellowish brown .Under 
the scope the colors were there, blue, red, yellow and black. But on the slide 
the tissue was that brownish yellow. The researcher does not like to strong 
yellow color. Since my control was fine I question if something was going on 
with their tissue. I do not know how the tissue was handled before it came into 
the lab. They were very calcified and were decaled for 1-3 days in Cal Rite. I 
do know they were not rinsed after decal and were put straight back into 10% 
NBF before I got them for processing.
Should I have used a human control instead of canine?  These were very large 
pieces that were crammed into the cassette.
Thanks for the help. Sorry for the long post.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
1101 Bates St.
Houston,TX
832-355=6524 (lab)
832-355-6812 (fax)



Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
texasheart.org | 

Re: [Histonet] Cryostat

[Morken, Timothy via Histonet]

Guess I missed your other questions. 

I think the fog mist (Cold D) may be better - it reaches all area of the 
chamber. UV light is shaded from some areas, though probably hits most areas 
you might be touching. 

The misting requires a specific solution, not just anything. Hydrogen peroxide 
is the disinfecting agent. 

Other features:
We have the vacutome on the original units but we never use it. We do all 
kidney and muscle sectioning and these small samples do not produce much 
debris. On our new replacement unit (to replace a 7-year old NX70) we opted out 
of the vacuum.  If using in a gross room/Or setting the vacuum may be very 
useful. It only adds a hose and suction manifold in the unit and does not get 
in the way of anything. 




Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Morken, Timothy via Histonet  
Sent: Wednesday, May 19, 2021 8:26 AM
To: Brittany Hethcox 
Cc: Histonet 
Subject: Re: [Histonet] Cryostat

Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're 
reliable and easy to use. We have the elevator on ours and it makes a 
difference for different size people in the lab. It also can have a vapor 
disinfectant system which we use and it works great. 

We use ours for kidney and muscle cryosectioning, not gross room/OR sections 
but these would work fine for that. They have a lot of room inside for 
specimens. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center


-Original Message-
From: Brittany Hethcox via Histonet 
Sent: Wednesday, May 19, 2021 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat

Hello everyone!

I am new to the Histology world. You all may know my previous coworker, Rhonda 
Ford. She officially retired and I am so happy for her! With that being said, I 
am in a pickle because the cryostat we had completely broke down. We were 
having issues with it during my training period, so I never officially got to 
use it. We are now looking for a new cryostat for our department. My supervisor 
is asking me about different models and features, but I don't know much about 
any of this. So I was hoping you all could help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically the 
"Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones 
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our purchase 
(e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

--
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

--
*"We Make Lasting Connections"*Henry Community Health 
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Re: [Histonet] Cryostat

[Morken, Timothy via Histonet]

Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're 
reliable and easy to use. We have the elevator on ours and it makes a 
difference for different size people in the lab. It also can have a vapor 
disinfectant system which we use and it works great. 

We use ours for kidney and muscle cryosectioning, not gross room/OR sections 
but these would work fine for that. They have a lot of room inside for 
specimens. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Brittany Hethcox via Histonet  
Sent: Wednesday, May 19, 2021 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat

Hello everyone!

I am new to the Histology world. You all may know my previous coworker, Rhonda 
Ford. She officially retired and I am so happy for her! With that being said, I 
am in a pickle because the cryostat we had completely broke down. We were 
having issues with it during my training period, so I never officially got to 
use it. We are now looking for a new cryostat for our department. My supervisor 
is asking me about different models and features, but I don't know much about 
any of this. So I was hoping you all could help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically the 
"Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones 
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our purchase 
(e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

--
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

--
*"We Make Lasting Connections"*Henry Community Health 
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[Histonet] Cryostat

[Brittany Hethcox via Histonet]

Hello everyone!

I am new to the Histology world. You all may know my previous coworker,
Rhonda Ford. She officially retired and I am so happy for her! With that
being said, I am in a pickle because the cryostat we had completely broke
down. We were having issues with it during my training period, so I never
officially got to use it. We are now looking for a new cryostat for our
department. My supervisor is asking me about different models and features,
but I don't know much about any of this. So I was hoping you all could
help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically
the "Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our
purchase (e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

-- 
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

-- 
*"We Make Lasting Connections"*Henry Community Health
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