My first thought about calciumcrystals is solving by the chromatic acid. I would recommend a vonKossa stain for calcium deposits.
Regards Gudrun Lang -----Ursprüngliche Nachricht----- Von: Akemi via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Dienstag, 9. August 2022 23:51 An: Histonet Betreff: [Histonet] Absence of Crystals in stains I received this from a pathologist friend and need your help. Unfortunately, I can’t attach the images he sent me on histonet. “I’ve got a histopuzzle for you. This is a fungus ball from a maxillary sinus. On the H&E, it is loaded with these birefringent crystals, which I hypothesize are calcium oxalate. On the GMS stain, however, the crystals are completely absent. Do you think there’s some reagent in the GMS procedure that dissolves crystals that would be resistant to tissue processing and the H&E stain? “ Thanks in advance! Akemi Allison BS, HT/HTL Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
