[Histonet] Histotech opportunities

[Stephanie L. Thompson via Histonet]

Sonic Healthcare USA has a great opportunity located in Somerset, 
Massachusetts. We are willing
to pay relocation for the right candidate. The labs is great and the people are 
very family oriented.

Check out our website at 
www.sonichealthcareusa.com

Apply through the link below:

https://shusa.wd5.myworkdayjobs.com/External/job/Somerset-MA/Histotechnologist_REQ-018151

You can also contact me directly at 210-428-1646 or 
sthomps...@sonichealthcareusa.com

Thank you - Stephanie Thompson


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Re: [Histonet] research questions

[Caroline Miller via Histonet]

I would say it depends on the project. I would always strive to get
controls from the same species, but sometimes that is just not possible. If
the marker's cellular or tissue location is easily recognizable then Ok
with the different species, but if it was a diffuse and hard to interpret
marker then I would try harder to get an in-species control.

I don't think I would use a different species control to determine ideal
concentration / protocol for the main cohort. I would get it going on the
control and run the cohort and tweak from there with positive samples
(hopefully) in the cohort.

Length of fixation/processing/age of slides of the control vs cohort is
also something to think about.

yours,
Caroline



On Tue, Feb 21, 2023 at 12:53 PM Charles Riley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> When performing IHC's for research projects is it recommended to use the
> same species for control tissues or will any tissues that the antibody has
> been validated for by the company be acceptable?
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-- 
Caroline Miller, M.Sc
Histology, Imaging, and Image Analysis Specialist
415 2187297
https://www.linkedin.com/in/mills42/
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[Histonet] research questions

[Charles Riley via Histonet]

When performing IHC's for research projects is it recommended to use the
same species for control tissues or will any tissues that the antibody has
been validated for by the company be acceptable?
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[Histonet] HepB positive tissue - question about safety

[M.O. via Histonet]

Hello,

We have some donor tissue that showed up positive for HepB on the Ultrio
Elite PCR test and Core Ab test, but was negative for the antigen test.

We have the samples in zinc buffered formalin. When processing this for
FFPE, is there a risk when working with this tissue for potential infection
to the histologist if there is an injury during sectioning?

Thank you,
Merissa
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