Re: [Histonet] Long term museum specimen storage

I don't know anything about "Jore's fixative" or the rationale of using a very hypertonic unbuffered 4% formaldehyde with magnesium, sodium, chloride and sulphate ions. If brown stuff is now bleeding out of your museum specimens, Jore Juice evidently isn't a good preservative. According to

Re: [Histonet] Histonet Digest, Vol 237, Issue 4

Thought on alternative for Sudan Black. I don't use this stain...an alternative is an Oil Red O stain. Oil Red O is done on frozen sections...but you can also deparaffinize FFPE sections to water, and then perform your Oil Red O stain. I hope this helps. Very Respectfully, Eddie Martin Eddie

[Histonet] Detergent in heating antigen retrieval

Dear histoneters, I have performed heating antigen retrieval with citrate buffer pH 6 with 0.05% tween-20, however I have seem recipes with no detergent, anyone has any experience or knowledge if it is better with or without the detergent? Thank you! -- Dr. Alonso Martínez

[Histonet] Long term museum specimen storage

Hi All, I am looking to replace the fixative for veterinary specimens that have been preserved as "museum specimens". They are kept in jars in a glass case outside our lab, however, some of the fixative is starting to turn brown (and we've pulled a few jars that have some slight cracks in