Re: [Histonet] Microwave Processing
Hello all, I use microwave processing exclusively. First of all, are you making sure that each reagent is completely extracted before the next reagent is administered? I know the tissue cannot be completely dry, but all residue reagent should be siphoned off before the next one goes in. This is especially important when dehydrating the tissue with ethanol, acetone, or polypropylene reagents. I usually do at least 3 resin changes if not 4. Curing the resin is another issue I've seen. Even though protocol says to cure for three days, I have found curing for a week is better. Especially important to cure the resin blocks longer in humid, rainy, or cold environments. Another issue is the diamond knives. Make sure that your clearance angle is correct and the water in the boat meets the knife edge, if you are doing TEM. Check those things and happy sectioning. Lita Sent from my Verizon, Samsung Galaxy smartphone Original message From: "Campbell, Tasha via Histonet" Date: 11/8/23 1:44 PM (GMT-06:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Processing Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=ZQs-KZ8oxEw0p81sqgiaRA=Z-Quiwr_oQfGYLNUELwQ1A=V2zfrROQEcGKlRUW4kujQYF33S2YA8Y2zc14D8heDFEQhQs5URz_aaD3SVRB-BEY=Abfh6qOZ3XnmvUsraeSWPUfFcn1Ow8IUH6TreF8Ivnk= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave Processing
They may make a combo UPS/line conditioner. That would be ideal. Line conditioners stabilize and protect against power spikes/surges. Thanks, Brian -Original Message- From: Campbell, Tasha Sent: Wednesday, November 8, 2023 12:40 PM To: Cooper, Brian Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwave Processing (EXTERNAL EMAIL) CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: tcampb...@fgamd.com. What does a line conditioner do? The company was bought out recently and basically has no employees so I have no one to help me. Tasha Campbell Sent from my iPhone > On Nov 8, 2023, at 3:36 PM, Cooper, Brian wrote: > > Hi Tasha, > > Maybe look into getting a line conditioner for your tissue processors. Your > tissue processor vendor may have some suggestions. > > Thanks, > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of > Pathology and Laboratory Medicine Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 > bcoo...@chla.usc.edu > > -Original Message- > From: Campbell, Tasha via Histonet > Sent: Wednesday, November 8, 2023 11:44 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL) > > CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside > CHLA. Do not open attachments, click on links, or respond unless you expected > this message and recognize the email address: > histonet-boun...@lists.utsouthwestern.edu. > > Is there anyone that is super familiar with microwave processing? I really > need some help. I am having trouble with my tissue and I think it's the > processing. I don't know what else it would be. We moved to a new building > so I don't know if the power could be stronger and its changing the way it > processes or what. I have tried messing with the program times and temps but > I cannot figure it out. My blocks keep having what looks like knife lines > but it's not the microtomes. The only change has been moving into a brand new > building. Any help would be appreciated! Thanks. > > > Tasha Campbell, B.S., HTL (ASCP) > Frederick Gastroenterology Associates > 7109 Guilford Dr. Suite 300 > Frederick, MD 21704 > 301-695-6800 ext. 144 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://secure-web.cisco.com/1xOy41N6xvg0UteDOIpjvHf_CApfMcmUGNNj458SM > -CGccqMtGH-FueUgDT2-PuUzi4tvd_GkWrTORVTZWS5BZJgE72NR2xJJTk01-cOaT-nzZM > tUbO3ZfdoGwdKFXuKi0Qq7wY6p0enhF_HVv0kxEW-8mnROFvKHXVmVK3ntIMO6OhYNRZVw > X-VTPtEydHHpARUr1BSXsHA3MtHNTBt4HykE0OXOK8asWk8RQW_yKcYjWxSEEwH_vD1ocX > Up48AzfKg8xNdL56OW30ndSRD5hmp0Gpp-mLx0fN5_ET34tkBsdiHLyQg0kk3pJOLACTze > dmSiOPdFtx_ZRJ-ytfdE1V08WztA5BHIJhSrt44_FhbVF5KKndReERS2HveVBcsPKFebUL > FXvEP72PvfysukCulHG3OpPnMFY_IXE2fkNA8_EQhQGsOmCVyg7Y9AICeRTtXwI9DytSpM > f7XCTujLDQ/https%3A%2F%2Furl.emailprotection.link%2F%3FbXyKyZCpvAJAuWZ > pEARN9oWjGEc0NHCYoC4VTztcJcf6GYOHMwDA04MoeflfYb1xPjKWMnZkaOCqeOtNoZ4Sw > kd-VyGHnZDhhsZd6fJhf9axt9VVVZ50GIwj4DnibSEV8tf2M7CLjuHYF9MkMPooCQ4Mt0P > ATuuXc0T64axJSX2gX7INjxTgJxGB_LdNp_wf2pRolRjTD6LT6OcXK7vrZgrIgSwnTljdL > T_anURFohI0k-BfXgqgcHF6KIYIK23zWTmIg3tG0Sbiybk0fQ_cSufokcCBEdzJ7X8qfla > g8Qfau0FsEpgdw5sDb49u1tC5rIVFbE7XPwklXgZA1K2hCImmIcuRZyO_TcmopBwK598tM > 1u4L5MHzmYR6AXF4ClgS7r2ofMK0RsiZIcnPq2daWo7Pfh6fs7eV1avQ-x0wojgsvi_XnL > is5CwLvu6oaRjdbCzHjtxYS2nfdzJYUEqHClxcA7Twh0DlyJ3vEf8iqCEdWX5p0nW9EvH9 > 11zINBjt0lUDflMBMXT4tkDigoynpB7nJH3RZDt9yBFTZ4HQSoYKUJcatXnLiZ2qw-tIY1 > GRcxdriVyv_XEubDtu2IsBa-S69JOaJXnnuwrK2EcEgHbXlTs-ZunA8_KfVoyBf6bj3K54 > 7D0LehZYi4ZTn3_l8jMqlPyw8-4eJ1SiU_iyIh4%7E > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential or > legally privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave Processing
What does a line conditioner do? The company was bought out recently and basically has no employees so I have no one to help me. Tasha Campbell Sent from my iPhone > On Nov 8, 2023, at 3:36 PM, Cooper, Brian wrote: > > Hi Tasha, > > Maybe look into getting a line conditioner for your tissue processors. Your > tissue processor vendor may have some suggestions. > > Thanks, > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 > bcoo...@chla.usc.edu > > -Original Message- > From: Campbell, Tasha via Histonet > Sent: Wednesday, November 8, 2023 11:44 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL) > > CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside > CHLA. Do not open attachments, click on links, or respond unless you expected > this message and recognize the email address: > histonet-boun...@lists.utsouthwestern.edu. > > Is there anyone that is super familiar with microwave processing? I really > need some help. I am having trouble with my tissue and I think it's the > processing. I don't know what else it would be. We moved to a new building > so I don't know if the power could be stronger and its changing the way it > processes or what. I have tried messing with the program times and temps but > I cannot figure it out. My blocks keep having what looks like knife lines > but it's not the microtomes. The only change has been moving into a brand new > building. Any help would be appreciated! Thanks. > > > Tasha Campbell, B.S., HTL (ASCP) > Frederick Gastroenterology Associates > 7109 Guilford Dr. Suite 300 > Frederick, MD 21704 > 301-695-6800 ext. 144 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://url.emailprotection.link/?bXyKyZCpvAJAuWZpEARN9oWjGEc0NHCYoC4VTztcJcf6GYOHMwDA04MoeflfYb1xPjKWMnZkaOCqeOtNoZ4Swkd-VyGHnZDhhsZd6fJhf9axt9VVVZ50GIwj4DnibSEV8tf2M7CLjuHYF9MkMPooCQ4Mt0PATuuXc0T64axJSX2gX7INjxTgJxGB_LdNp_wf2pRolRjTD6LT6OcXK7vrZgrIgSwnTljdLT_anURFohI0k-BfXgqgcHF6KIYIK23zWTmIg3tG0Sbiybk0fQ_cSufokcCBEdzJ7X8qflag8Qfau0FsEpgdw5sDb49u1tC5rIVFbE7XPwklXgZA1K2hCImmIcuRZyO_TcmopBwK598tM1u4L5MHzmYR6AXF4ClgS7r2ofMK0RsiZIcnPq2daWo7Pfh6fs7eV1avQ-x0wojgsvi_XnLis5CwLvu6oaRjdbCzHjtxYS2nfdzJYUEqHClxcA7Twh0DlyJ3vEf8iqCEdWX5p0nW9EvH911zINBjt0lUDflMBMXT4tkDigoynpB7nJH3RZDt9yBFTZ4HQSoYKUJcatXnLiZ2qw-tIY1GRcxdriVyv_XEubDtu2IsBa-S69JOaJXnnuwrK2EcEgHbXlTs-ZunA8_KfVoyBf6bj3K547D0LehZYi4ZTn3_l8jMqlPyw8-4eJ1SiU_iyIh4~ > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential or > legally privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave Processing
Hi Tasha, Maybe look into getting a line conditioner for your tissue processors. Your tissue processor vendor may have some suggestions. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu -Original Message- From: Campbell, Tasha via Histonet Sent: Wednesday, November 8, 2023 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL) CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-boun...@lists.utsouthwestern.edu. Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://secure-web.cisco.com/1iXiR7DgxMp8b_BUwsOCVLYh5BrZ18oRqXPXSrsq6yyJTaeSklH-M9e_nxo0H1edrZXY8yW2gB8uBPQFlrkycg81-6fiZfp9m4ew3W1GIWPzMJxjQlg5CqwTVlRq0Cyjnm_YOVGfYZOiimFv4mFXsvQ4s6Z5mi6VGMQ9l9EV181iFfWy_EdrhCytI8lcrPR5T3NjeUxeI0fA9cFJZ7LWxqU--onyetKWST0IMK268d0AjEQ7OSLKvdYXVmYyVJAzxfipn9ViDmsCqXJRxf4uITFghZAI2yDQKiuWScym6vAcxOar9XYPwUrdH57HihsGPipJmQxk7mxyMmOsxjl-Hao_xLjG2Vk5YaQwnVbDu_3Wghi1tH8eSyr9-aN3yczudiSqKuNpUfLXBO5tnIwNkVhpnspiPX0-LrFtTxl5TTZGGeAUdxUxy3O4osa6kJqifCnXi6k8ZX46q32cw2YH4vw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave Processing
Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
