Ion Exchange Decal containers will change your experience forever.
-Original Message-
From: Gudrun Lang via Histonet
Sent: Friday, January 26, 2024 3:32 AM
To: 'Chakib Boussahmain'
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Bone samples
Hi,
In my opinion the hardness of the decalcified blocks is often rather due to the
paraffin-processing than the residual calcium. Especially when the tissue is
decalcified really long. The hardness comes from the dehydration and "cooking"
of collagen fibers. So additional decal will not help reducing the calcium, but
helps to reintroduce water into the collagen-grid. And this is helpful for
softening and cutting.
For myself, I often scratch the paraffin on the blocksurface away to face the
bone directly to the water. Then I let them "swim" on my waterbath, until the
surface is turned rather milky. After cooling again I cut in very very small
steps to trim the surface. Sometimes it needs repeated swimming and cooling
(and patience) to get a rather acceptable section. It is advantageous to pick
them up on adhesive slides and let them dry in an 60°C oven to get rid of any
residual water under the section.
Hope this helps
Gudrun
-Ursprüngliche Nachricht-
Von: Chakib Boussahmain via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Mittwoch, 24. Jänner 2024 23:34
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Bone samples
Hi guys,
I hope this messagefinds you well. I am currently working on a study involving
bone samples thathave been treated with slow decal and embedded in paraffin. I
am facingchallenges in obtaining nice sections, and I was wondering if you
could providesome guidance or recommendations.
Thank you in advance for your help!
Chakib
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