Re: [Histonet] picric acid

2016-05-06 Thread E. Wayne Johnson via Histonet
I read "Chemical Magic" and the "Anarchist's Handbook" in high school many years ago. That was back when boys carried pocket knives and sometime took their shotguns to school to show their friends. I still occasionally make a little bit of NI_3 for fun. We've tried to make picric acid explod

Re: [Histonet] Pap stain without xylene

2016-05-26 Thread E. Wayne Johnson via Histonet
Xylene is becoming more and more of a nuisance material and a problem for us in use and in disposal. We are still able to use it but with increasing concern. We have been able to eliminate xylene from our staining procedures altogether in our small laboratory. We use a hair dryer to dry the

Re: [Histonet] PIG processing tissue

2017-09-06 Thread E. Wayne johnson via Histonet
We routinely examine pig heart as part of diagnostic histopathology and generally we are looking for the lesions of Vitamin E / Selenium deficiency. Vitamin E and organic Se are expensive and often deficient in commercial piglet diets. We simply fix with a formalin based fixative and proces

Re: [Histonet] Processing Veterinary Samples

2019-04-16 Thread e wayne johnson via Histonet
The vet samples can be processed just any other animal tissue whether they be human or rat. People is animals too. Formalin fixed there are no special safety issues. E. Wayne Johnson DVM Enable AgTech Beijing ewj Email:e...@pigs.ag Signature is customized by Netease Mail Master On 04/16/2019 08:

Re: [Histonet] Tissue Tek TEC 6 Embedding Centre

2019-05-30 Thread E. Wayne Johnson via Histonet
I am skeptical that parts are not available for these relatively new (fifteen years old) machines... Of course reliability is a serious concern in any case. E. Wayne Johnson DVM Enable AgTech Beijing Etheridge, Sandra AGRI:EX via Histonet wrote: Hi everyone, We are looking to purchase a new

Re: [Histonet] UV LIGHT BOX to kill BLOODBORNE PATH

2019-11-23 Thread E. Wayne Johnson via Histonet
I really liked that idea of having a secondary copy of the paperwork to take to any potentially dirty area. I am not very excited about UV light as a disinfectant for paper. If the paper is dirty, it's dirty. Incineration is a great disinfectant method. Fire is another good one for paper (as a s

Re: [Histonet] On-line references

2020-03-26 Thread E. Wayne Johnson via Histonet
And I appreciate Bryan Llewellyn and the other old experienced hands and I really like Gray and Humason and even Lillie, and the others and the way they wrote and the way they thought, and their delight and fascination with the world they were discovering. E. Wayne Johnson Enable AgTech Beijing

Re: [Histonet] FW: Microtome at home

2020-04-16 Thread E. Wayne Johnson via Histonet
"Taking control of the situation is key." It's very interesting to me that most of the responses have to do with institutionalized bureaucratic ideas (safety, insurance, liability, regulations) rather than how to get things done. I was thinking about how Virchow and Henle and other pioneers woul

Re: [Histonet] Formalin fixation for COVID-19 positive tissues .....

2020-04-20 Thread E. Wayne Johnson via Histonet
Where aldehydes are used for disinfection we consider 30 minutes contact time for difficult viruses like African Swine Fever (ASF) virus to be adequate.  Coronaviruses like PEDv TGE IBV and the SARS virus are much easier to inactivate than ASF virus. I'd consider the SARS virus to be similar to

Re: [Histonet] Tissue processor errors, failures and what to do

2020-07-04 Thread E. Wayne Johnson via Histonet
Automation is a wonderful thing but it is only a replacement for what people used to do by hand. We have incubators that can be set to 60C and we have a Rube Goldberg-ized microwave oven with a thermal controller and relays (and the not-to-be-forgotten flyback diode) and a K-type thermal probe c

Re: [Histonet] Blades

2020-11-17 Thread E. Wayne Johnson via Histonet
In China we can buy Leica, Feather, or domestic. The domestic ones are not uniformly good or bad. I am really really tired of Feather and I consider that my time is worth something so I insist that we use Leica although they are as "high as a cat's back". > ---Original Message--- > From

Re: [Histonet] Maximum and Minimum Temperatures on Embedding Center Paraffin Tanks

2020-12-08 Thread E. Wayne Johnson via Histonet
You could warm the paraffin in an oven overnight to melt it so that the heat of fusion is not extracted from the embedding center paraffin tanks when it is added as melted paraffin at 58-65 degrees. Pairan, Kelly via Histonet wrote: Good Afternoon, Recently we starting taking the daily maximum a

Re: [Histonet] Dragon

2021-02-10 Thread E. Wayne Johnson via Histonet
I use Nuance Dragon for making video scripts, transcribing audio stripped out of video recordings, and for dictation of documents. Until one gets it trained it will create very humourous mondegreens of technical terms. But you can add terms like thromboembolic meningoencephalitis and extramedul

Re: [Histonet] release of body parts

2021-08-18 Thread E. Wayne Johnson via Histonet
I wanted my femoral heads after a dual hip replacement. I was refused as it was against local policy but the surgeon was kind enough to take some pictures for me and sawed through one sagitally so that i could see the pathology. E. Wayne Johnson DVM Enable AgTech Beijing Cartun, Richard via Hist

Re: [Histonet] release of body parts

2021-08-19 Thread E. Wayne Johnson via Histonet
I will take the other side of this argument. If you go to the Dentist and he extracts a tooth, it is the usual procedure that he gives it to you. After all it is "your tooth". Like wise, it's your gall bladder.  The legal department should understand that it is your personal property and the m

Re: [Histonet] The LEGAL side of specimen ownership

2021-08-19 Thread E. Wayne Johnson via Histonet
Townsend synth intro [dito dito dito dito nano dito nano dito] Washington University vs WJ Catalona (2005, 2008) dealt with the subject of research samples which had been formally donated to the university, not with body parts excised during the course of surgery that the patient wants to

Re: [Histonet] Cold ischemic times

2022-02-15 Thread E. Wayne Johnson via Histonet
When I first saw the title of your email I thought it was a publication OR a commentary on The Era. New York Times Cold Ischemic Times Global Times Los Angeles Times. * It was the worst of times it was the best of times Man, these are such cold ischemic times, (Brother, can you paradigm?) We