Re: [Histonet] Digital Pathology & Coverslipping

2017-08-14 Thread Haley Huggins via Histonet
Glass or film coverslips are fine, but you have to make sure they are clean, no excess mounting media, or bubbles. The scanners pick up a lot of extra things you don't want scanned. Also, check with your pathologists to see if they have an opinion one way or another about which coverslips they

[Histonet] IHC for Lupus

2017-07-21 Thread Haley Huggins via Histonet
Is there an IHC antibody that tests for Lupus? Does anyone know? If so which? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] scrubs/business casual

2017-06-21 Thread Haley Huggins via Histonet
We wear scrubs. However, we are a small reference lab and our employer bought us 4 sets of scrubs w/embroidered company logo to wear M-Th. I added free-scrub Friday, so we can wear whatever scrub set we want to wear on Friday purchased on our own. On Jun 21, 2017 6:51 AM, "O'Donnell, Bill via

[Histonet] CPT Code for PGP9.5 free floating tissue stain

2017-06-22 Thread Haley Huggins via Histonet
I am running into an issue with billing and which code is correct for staining PGP9.5. Is it 88342? Also the billing guy asked me if thr secondary goat, anti-mouse secondary antibody could be billed, can it? I would appreciate any help I can get as soon as possible! He needs to bill out today.

[Histonet] Question regarding frozen control tissue

2017-06-08 Thread Haley Huggins via Histonet
I am trying to find a way to obtain small bowel/intestine as a fresh/frozen sample to be able to use it for a control with my staining for PGP9.5 free floating IHC stain. Does anyone know where a good place would be to obtain the needed control tissue? *Haley Huggins, HT (ASCP)cm* *Technical Lab

Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

2017-10-06 Thread Haley Huggins via Histonet
I would also be interested in knowing this tip if anyone knows it. I will also have to check out that book. We only do cryostat sections at our lab. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* On Fri, Oct 6,

[Histonet] Developing a multiplex cocktail

2017-10-06 Thread Haley Huggins via Histonet
Hello Histonetters, It has been years since I had to create a multiplex of a dual-antibody cocktail and for the life of me am having a brain block on the best way to go about this. Does anyone have or know of a procedure to make this type of cocktail? *Haley Huggins, HT (ASCP)cm* *Technical Lab

Re: [Histonet] QIHC Study Materials

2017-10-17 Thread Haley Huggins via Histonet
I sent to Lindsey in a private email which I thought I had hit reply all that Michigan Society of Histotechnology also has a study guide for the IHC, but you are right, Jay, that the Dako IHC manual is your best study guide. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas

Re: [Histonet] Histonet Digest, Vol 167, Issue 12

2017-10-17 Thread Haley Huggins via Histonet
My suggestion for using the Dako IHC manual is not necessarily just about the automated application, but about the antibodies themselves and what the end results are supposed to be. It isn't the only thing you should use to prepare for the QIHC exam, but if you want to learn about a large amount

Re: [Histonet] Type of Nitrile Gloves in your lab

2017-11-07 Thread Haley Huggins via Histonet
I like the Halyard brand. We also use Confiderm from McKesson, those are pretty good too. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* On Mon, Nov 6, 2017 at 10:25 AM, Cooper, Brian via Histonet <