Is the humitity causing any problems or is the number just not what you're
used to seeing when you look at the readout?
On Tue, Aug 30, 2011 at 10:08 AM, Jill Cox jco...@yahoo.com wrote:
Hi Histonetters!
I am having humidity issues in a new lab in Long Beach Ca. It's 68%
humidity inside lab.
I think normal breast staining of myoepithial cells is the best control for
this antibody. We use BioGenex's antibody (product # MU333-UC) at 1:200
on the Benchmark XT (CC1 mild, 8 mins primary antibody and ultraview DAB
detection).
Mark
On Fri, Mar 16, 2012 at 7:34 AM, Rene J Buesa
FISH should be scored at high power under oil immersion (60-100x
objective). If they are scoring at 40x, it could be a problem.
good luck!
Mark Tarango
On Tuesday, June 5, 2012, Eric Hoy wrote:
We do a LOT of fluorescent microscopy in our immunology lab, so I have a
bit
of experience
I'm trying to think this through. Like Will said, if it can be shown that
you can charge immunos per block... I thought we could only charge IHC per
specimen these days. Wouldn't each stage be a different specimen? I would
think billing per block of the same stage would be over charging. Why
Carol,
Its nice to hear this isn't a regular thing. In reading your original
question, it sounded like you were excited to be charging five times for
those immunos and you were ready to argue for it. Apparently that was not
the case, you wanted more information and thoughts on the subject.
I
I would think that if you're billing the client and not the insurance that
you could charge per block for the technical. After all you're just
providing the stain to them. In my opinion, the client should eat this
cost. I would let the client know that you'd be billing this way before
staining
Hi Willem,
H. pylori needs the low pH of the stomach to survive and grow. It won't
naturally be in lung tissue. When looking for HP, it's found on the mucosa
and gastric pits of the stomach. Since you're looking for it in a specific
place, I think putting it in lung is a bad idea.
Your best
Hi Cindy,
The one from Dako works well at a 1:200 dilution on the Ventana platforms
(catalog #M7001). We use HIGH GRADE ovarian serous carcinoma as a positive
control. You should get intense nuclear staining throughout the tumor with
this control.
Mark
On Thu, Jul 19, 2012 at 12:25 PM, Cindy
Hi Donna,
We use Dako #M3635 at 1:100. Mantle cell lymphoma is our control.
Mark
On Tue, Jul 24, 2012 at 8:16 AM, Suresch, Donna L.
donna_sure...@merck.comwrote:
Hello Histonetters,
Has anyone done IHC using Cyclin D1 antibody? What vendor was used? What
control tissue was used?
Thank
Hi Debra,
Formaldehyde was listed as known to be a human carcinogen in the 12th
Report on Carcinogens (2011) put out by the Department of Health and Human
Services. Here is a link
http://ntp.niehs.nih.gov/ntp/roc/twelfth/profiles/Formaldehyde.pdf
This is what is probably behind any recent
Hi Carol,
Does this person put them in a freezer for 20 mins or so after DAPI
staining? Someone once told me this helps with smearing. I'm not really
sure if its true, but couldn't hurt to try it.
Mark
On Thursday, August 30, 2012, Barone, Carol wrote:
Someone sent me a question regarding
dispensers are from that lot. The recall letter
should have been received... he could look for that too...
Mark Tarango
On Wed, Sep 9, 2009 at 4:14 AM, Sally Price sprice2...@gmail.com wrote:
On behalf of a colleaugue who doesn't participate in the Histonet, I'd like
to ask folks if there experiencing
the new labels but they did help our IT people figure out how to do it.
Mark Tarango
On Wed, Sep 9, 2009 at 12:18 PM, Michael Mihalik m...@pathview.com wrote:
I need some help from those of you who work with Ventana stainers.
The Ventana equipment requires the use of Ventana slide labels. You can't
that the barcode itself can
serve as the second identifier of the specimen.
Mark Tarango HT(ASCP)QIHC
On Wed, Sep 9, 2009 at 1:11 PM, Michael Mihalik m...@pathview.com wrote:
Ventana’s ebar printer – tell me a little about that, please….
I don’t think I’m going to like what I hear, but let me
labeling.
Mark Tarango
On Wed, Sep 9, 2009 at 4:44 PM, Michael Mihalik m...@pathview.com wrote:
Yeap, news travels fast. I had heard of this as well.
For us and our system design, however, the issue is labeling the slide
correctly AT THE TIME THE SLIDE IS CUT. I hate that you have to either
the
labels on the slides. Then they go over to the waterbath and cut the
orders.
Since we only have two location where Ventana labels can be printed, the
tech has to get up and to go print them. At the cutting station there is
only a slide label printer for LIS/Powerpath labels.
Mark Tarango
We've been getting these requests lately too. I've been wondering if we
need to monitor fixation time for these specimens, since Her2neu is a
possibility.
Mark Tarango
On Mon, Nov 2, 2009 at 9:44 AM, Maray Weirauch mweira...@crittenton.comwrote:
We have had some oncologists asking for HER2
We use the polyclonal from cellmarque. 1:200 on the Ventana XT. We use CC1
mild and then do protease 2 for 8 minutes to get a nice clean stain.
Mark Tarango
On Tue, Nov 10, 2009 at 1:41 PM, Taylor, Jean jtay...@meriter.com wrote:
Hi everyone,
Just trying to get an idea of where labs
Your goal is not to have floaters. If you get one, your policy should set
out to determine the cause of these incidents. You should track who did it
(in a spreadsheet), where it happened (grossing, embedding, cutting...).
Then you should have a meeting every so often with people from the lab and
!
Thanks
Mark Tarango HT(ASCP)QIHC
Cellnetix Pathology
1124 Columbia Street, Suite 200
Seattle, Wa 98104
On Thu, Jan 7, 2010 at 9:50 AM, Hammel, Vicky vham...@primecare.org wrote:
What are labs using for positive tissue for CMV IHC? If anyone is
interested
in trading tissue (slides or blocks
Sorry everyone, I didn't mean to send that to the whole histonet. Please
respond privately Vicky.
On Thu, Jan 7, 2010 at 10:12 AM, Mark Tarango marktara...@gmail.com wrote:
I can trade you. I have a few prepared control blocks that we use for
IHC. I'd be willing to trade for malignant
Are you really running special stains on the Intellipath?
Mark Tarango
On Wed, Jan 27, 2010 at 7:11 AM, Blazek, Linda
lbla...@digestivespecialists.com wrote:
I love the Intellipath from BioCare. I've had mine for over a year. It's a
fully open system, it has the ability to continually add
The inform HPV16 is a DNP labeled probe. You should use the ISH Iview plus
detection kit for staining.
Mark Tarango HT(ASCP)QIHC
On Wed, Feb 17, 2010 at 10:35 AM, Marian Powers mpow...@dpspa.com wrote:
Hi: Any one out there running HPV 16 insitu on the Benchmark XT. I need
some help
Does anyone know if the ruling about BRCA1 and BRCA2 affects MTM labs /
Cintec's monopoly on the p16 antibody?
Thanks,
Mark Tarango
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minutes then 8 minutes with anti-RCC, counterstain and bluing.
Mark Tarango
On Fri, Apr 2, 2010 at 10:13 AM, Howery, Jeffrey jhowe...@yrmc.org wrote:
What are people using for a Renal cell Carcinoma. ( this is a test )
Confidentiality Notice: This e-mail message, including any attachments
that the pathologists did all the grossing.
Just brings back memories. Thought I'd share.
Mark Tarango
On Mon, Apr 5, 2010 at 10:03 AM, Andrew Burgeson nap...@siscom.net wrote:
The point is not about gender, as I stated before...
It's about a person's health risks and lack of training
had much more trouble with
Ventana's negative rabbit serum before they finally discontinued it and
switched to a new product.
Mark Tarango
On Wed, Apr 7, 2010 at 1:37 PM, Gagnon, Eric gagn...@kgh.kari.net wrote:
Having just validated and started using Biocare Universal Negative Control
Serum
Hi Histonetters,
I remember a while back someone asking about this. I just wanted to post
for anyone having trouble with Ventana's ultraview kit (mast cell staining)
that when you use a combination of CC1 and protease, the mast cell staining
goes away.
Thanks
Mark Tarango
I forgot to add: I was talking about pankeratin staining in sentinal nodes.
On Wed, Apr 7, 2010 at 2:05 PM, Mark Tarango marktara...@gmail.com wrote:
Hi Histonetters,
I remember a while back someone asking about this. I just wanted to post
for anyone having trouble with Ventana's ultraview
x3 only because you can't tell the difference between these basal cell
markers CK5 and CK14 (they stain the same cells and both in the cytoplasm).
Mark Tarango
On Fri, Apr 9, 2010 at 8:31 AM, Cheri Miller cmil...@physlab.com wrote:
What are people charging Pin 4 p63,CK5-14and P504S..88342
Hi Cindy,
My lab uses KP-1.
Mark
On Fri, Apr 30, 2010 at 8:48 AM, Cynthia Pyse cp...@x-celllab.com wrote:
Happy Friday Everyone
What clone is everyone using for the CD68 antibody for FFPE human tissue?
Thanks for the info in advance. Everyone have a great weekend.
Cindy Pyse, CLT, HT
You would think the ASCP would have some kind of grossing tech qualification
or certification for those who aren't PA's but have the 60 semester hours
and training. Just thought I'd throw that out there.
Mark Tarango
On Wed, May 19, 2010 at 7:36 AM, Jeter, Brent
brent.je...@gwu
I just realized the question came from Belgium. I have no idea how they do
things there.
Mark
On Thu, May 20, 2010 at 2:14 PM, Mark Tarango marktara...@gmail.com wrote:
Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if
you do HER2 the maximum is still 48 hours. I'm
Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if
you do HER2 the maximum is still 48 hours. I'm assuming you want HER2 as
well, so your best option would probably be to hold the tissues in 70%
alcochol on the processer until Sunday night.
Mark Tarango
On Thu, May 20
I heard on a teleconference yesterday that they're going to be changing both
to 72 hours max time fixation, but until its the published guidlines 48
hours still stands for her2neu.
Mark
On Thu, May 20, 2010 at 2:28 PM, Mike Pence mpe...@grhs.net wrote:
The problem with all this is that the
Hi Phebe,
I can't be sure about this since you didn't post your protocol, but
alpha-SMA is an antibody that does not require antigen retrieval. If you're
doing some kind of retrieval, I'd suggest trying it without.
Thanks
Mark
On Wed, May 26, 2010 at 6:56 PM, Phebe Verbrugghe
You'll have to use prep kit stickers and duplicate protocols to do it, but
it is possible. You just need to copy the protocol and save it as another
number, then change the primary antibody to a prep kit sticker save again
and then put that sticker on the dispenser. Then you need to print
Biocare has a rabbit polyclonal anti-HP ab and a mouse monoclonal. Which
one do you use?
Mark
On Wed, Jun 30, 2010 at 8:05 AM, McMahon, Loralee A
loralee_mcma...@urmc.rochester.edu wrote:
Use the RTU from Biocare. It is clean and easy. You can use it with the
Dako Flex Kit.
Loralee
Hi Cathy,
I'm using their p63, but on the Ventana XT with ultraview DAB detection. We
use it at 1:200 and get a strong signal in both breast and prostate
tissues.
If you haven't tried staining various cases of prostate, I'd suggest it.
Sometimes I'll get a prostate that stains the basal cells
Ethanol/alcohol is what will process the specimen. If the tissue is fixed
would it really matter that the tissue came into contact with ethanol?
Mark
On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB)
randi.ha...@horizonnb.ca wrote:
At a recent conference, our PA learned of using GEWF
Hi Norm,
It sounds like he had something to contribute. I don't think It shouldn't
matter where he works. Someone else asked the question.
Mark
On Mon, Oct 29, 2012 at 10:00 AM, Norm Burnham norm.burn...@propath.comwrote:
I didn't know that the Histonet site is being used as a medium for
Have you tried using more sections during extraction? Can you extract into
a smaller volume?
On Friday, November 2, 2012, Vanessa Orsini wrote:
Hello,
I need to extract RNA for a RT-PCR after Laser Micro
Dissection on xGal stained slides.
I tried using sections from unfixed frozen
Hi Jill,
Have you tried using cases that you've already sent out to other labs and
they've reported as ALK-positive? That is how we validated this assay in
my lab. Most cases aren't very positive (you only have to find 15 cells
out of a hundred that you count to call the case positive if a
...so even if I increase the number
of slides I'll never have a lot of material...
Inviato da iPhone
Il giorno 2 nov. 2012, alle ore 16:44, Mark Tarango
marktara...@gmail.com javascript:_e({}, 'cvml',
'marktara...@gmail.com'); ha scritto:
Have you tried using more sections during extraction
Francie,
Conjugated antibodes CAN be detected by secondary antibodies. So the
conjugation doesn't really hide the primary antibody to the secondary.
I've done it and seen it with biotin and FITC labeled antibodies.
Something to consider..
Mark
On Wed, Nov 7, 2012 at 11:43 AM, Frances Elizabeth
Hi Cynthia,
We do PCR for KRAS and BRAF. You cant do these by FISH anyway, the
mutations are too small.
We do FISH for ALK and HER2. For ALK, there is no antibody that can
detect all the positive cases. ALK FISH is the only method that can detect
ALK break aparts regardless of the fusion
Hi Vicki,
I don't have a protocol but it should be very similar to the HER2 dual ISH.
I'm assuming that you'll be using a probe targeted to chromosome 12 too
and not just doing the MDM2 (but maybe I'm wrong). I would start there.
I'm working on validating MDM2 FISH and it's nearly identical to
It can't be used to just pull blocks. The slides have to be reviewed and
the best block chosen by a pathologist. If there is only one block then
the pathologist needs to look at the slides and determine if there is
enough tissue for molecular testing. It's a professional charge.
Use it on
The staining portion is not high complexity. The reading of the slide is.
On Tue, Jan 22, 2013 at 10:04 AM, Courtney Pierce
courtney.pie...@quintiles.com wrote:
Are IHC high complexity test.
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating the
If anyone has any documentation that says the staining of IHC slides is NOT
high complexity it would help a histonetter out there. I got an e-mail
from someone who is HT(ASCP)QIHC but does not have an AA degree. Their lab
director is threatening their job saying they aren't qualified to do IHC
Am I the only one who thinks its funny Joe the Toe did an unsubscribe
e-mail and Cheryl Kerry copied an entire digest to reply. Oh histonet pet
peeves... haha
On Mon, Feb 4, 2013 at 1:05 PM, Cheryl tkngfl...@yahoo.com wrote:
Oh no, Joe! Say it ain't so!! Please tell us this is just to take
protocol (specially if it is based on DAKO'sprotocol)
should remain as is.
The diagnosis differences should not determine a change.
René J.
*From:* Mark Tarango marktara...@gmail.com
*To:* Wilson A wilson6...@yahoo.com
*Cc:* histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Hi Cheryl,
We use the Aperio system for HER2 scoring. Our lab manager put a cytotech
in charge of validating the digital reading and her next project is ER and
PR. For HER2 IHC, the software is initially set for Dako's Hercept test.
We don't use Dako, we use Ventana staining platform and the
of tumor.
Mark
On Wed, Feb 6, 2013 at 7:03 AM, Bob Richmond rsrichm...@gmail.com wrote:
Mark Tarango notes:
Many pathologists, if they have any doubt about the score will just say
that it is 2+ so that its gets HER2 by FISH which is considered the best
method for determining HER2 status.
On one
If you are just staining the slides and not reading them, then you are NOT
performing high complexity testing. The person who reads the slide is
doing the high complexity part.
Mark
On Wed, Feb 6, 2013 at 11:54 AM, Sara Baldwin/mhhcc.org
sbald...@mhhcc.orgwrote:
Hi histonetters
Is ventana
Just to clarify, this is not my interpretation. This is what CAP will tell
you when you give them a call.
Mark
On Wed, Feb 6, 2013 at 1:07 PM, Jesus Ellin jel...@yumaregional.org wrote:
I would say this is high complexoty testing and the tech performing this
has to have knowledge of the
Center
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [mailto:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Wednesday, February 06, 2013 2:07 PM
To: Jesus Ellin
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] High
Hi Jim,
We run the control slide that Ventana sends (although not the best control)
but we add a section from our tissue control block before staining. The
tissue control block contains a 0+, weakly staining 1+, and a 3+. We use
the ventana/tissue control slide as our batch control. This slide
I second that!
On Tue, Feb 12, 2013 at 11:12 AM, Robert Schoonhoven
robert_schoonho...@yahoo.com wrote:
All you need to do is have them drain the chamber and place the cassettes
on a nonabsorbent surface and allow them to cool to room temp. On Monday
morning have the techs put the into the
The package insert for PathVysion (HER2 FISH) says to bake overnight at 56
degrees C. We never do this and our signals are clear, bright, and
punctate. Many of the slides are just air-dried. Some are baked for about
20 minutes and I don't notice any difference between them. I wonder if I
would
Hi Matthew,
We use them and they work fine. They are much cheaper too. We've had them
for about 3 years. I initially had some concerns about the DAB being
absorbed and staining the labels on some cases. Our safety person told me
that it's not a safety concern and it's safe to touch the label
We use a heat block during digestion. There is less chance of
contamination with a heat block than with a water bath.
... and no we don't use antigen retrieval solution for this! We use a
Qiagen kit too.
Mark
On Wed, Apr 3, 2013 at 9:11 AM, Helen Fedor hfe...@jhmi.edu wrote:
We have been
11, 2013 at 6:26 PM, Cristi Rigazio cls71...@gmail.com wrote:
We have a formaldehyde test kit. It's a dip stick type test.
Sent from my iPhone
On Apr 11, 2013, at 5:31 PM, Mark Tarango marktara...@gmail.com wrote:
Can I ask how you test before dumping?
Thanks
Mark
On Apr 11, 2013 6:21
I'd like to add that we use the Aperio system with Ventana's antibody and
it works well. It took a lot of work tweaking of the algorithm to get the
software to score accurately using the 4B5 clone but that was the stain our
pathologists were already used to.
Mark
On Tue, Apr 23, 2013 at 1:50
Yes, if the stain can wait get some mount quick from Newcomer. There is a
good chance the tissue will fall off if you proceed with the tissue on
regular slides. I would wait until I get the mount quick.
Everyone else reading this should order a tube now for emergencies. You
will find yourself
Hi Kris,
The polymer backbone for this detection kit is conjugated with anti-rabbit
antibodies only (AP Polymer reagent). The specific probe is a rabbit
anti-mouse antibody in the AP probe reagent. That is why you only need to
apply the AP probe when your primary antibody is from mouse but not
Hi Elizabeth,
Room temp is fine for FFPE tissue for DNA extraction. We do it this way
every day!
thanks
Mark
On Tue, Jul 30, 2013 at 12:02 PM, Elizabeth Cameron
elizabeth.came...@jax.org wrote:
We are preparing paraffin slides for DNA extraction, and I am not sure if
once cut they should
My understanding was that this is just for Medicare patients...
On Mon, Jan 6, 2014 at 12:35 PM, Johns, Jill jjoh...@cpallab.com wrote:
The following was taken from the NCCI manual, Chapter 10, effective 1/1/14:
9. The unit of service for in situ hybridization reported as CPT codes
88365,
Here I am answering my own question. I just remembered where I used to get
this antibody. It's from Millipore/Chemicon.
Mark
On Mon, Aug 9, 2010 at 7:53 AM, Mark Tarango marktara...@gmail.com wrote:
Hi Histonet,
Does anyone use an Anti-IDO on FFPE tissues? Would you please let me know
I've been told by a Biocare Salesperson that the BC4A4 clone is the same
exact clone as 4A4. The BC in front of 4A4 just means Biocare. I don't
think that Ventana sells a concentrate of this antibody. You'd want a
concentrate for your PIN4 so you're probably better off sticking with your
Hi Nita,
I agree that buffer should be used as the negative control reagent. If you
have a seperate slide that is cut and put into buffer, just coverslip it
with the same mounting media and you have a negative control. All the
instrument is doing is putting on the antibody and then rinsing it
Hi Jessica,
Our control for this antibody is normal lung, adenocarcinoma of the lung,
and thyroid. We use this same block for other stains too (TTF-1,
sufactant-A, EMA, etc.) but if I were going to make a control block specific
for this antibody, I would want to include a squamous cell carcinoma
* *I think the chatter just died down since many of us are at the NSH
symposium.
Mark
On Wed, Sep 29, 2010 at 8:49 AM, Feher, Stephen sfe...@cmc-nh.org wrote:
I am having some issues with receiving email from the listserv. I was
successfully receiving email and it suddenly stopped. If I
Hi Vikki,
It's best to run it all together and run a negative control for each
detection kit.
Mark
On Fri, Oct 15, 2010 at 7:25 AM, Victoria Baker bakevicto...@gmail.comwrote:
Hi
I have a hypothetical question to those who run IHC on Ventana instruments.
Are you running your negatives with
Hi Sarah,
It's better to have the control on the same slide. There are slides that
work and slides that don't. You know which ones are good and which ones are
bad because you have that control on each slide. It's not always a complete
run that fails. Yes, you CAN have a single batch control
Hi Melissa,
We use it on bone marrow biopsies all the time on the Ventana XT. We made
sure to include BMBXs in the validation. We do get strange staining from
time to time, so it's particularily important to look at the negative
control for these.
Mark Tarango
On Tue, Oct 19, 2010 at 7:49 AM
Hi Justin,
Do you run the p53 on a cell block or a cytospin or something different?
Mark
On Mon, Nov 8, 2010 at 12:36 PM, Justin Peters
jpet...@bostwicklaboratories.com wrote:
We receive requests from our pathologists for p53 IHC on urine but I am
unsure as to what controls to use. We
Hi Gloria,
Are you still looking for some good HP controls? I could send you a block
of one of our controls if you're intersted. I have blocks from
several different specimens. Just let me know your address and fedex
number.
Thanks,
Mark
On Sun, Nov 21, 2010 at 12:30 PM, Gloria Cole
background
staining that I've seen?
I'd appreciate any info at all.
Thanks
Mark Tarango
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...@wvhcs.orgwrote:
Pax-2 (rabbit poly) is available from Cell Marque but in our experience it
also has some cytoplasmic background staining.
Mike Dessoye
---
Message: 21
Date: Tue, 4 Jan 2011 16:06:56 -0800
From: Mark Tarango marktara...@gmail.com
Subject: [Histonet] Pax-2
To: histonet
Hi Paula,
You mentioned the staining in a liver specimen using the cell marque
antibody against TTF-1. The clone named 8G7G3/1 that cell marque sells is
known to stain liver cells and liver cancer. Your pathologist might be
interested in knowing that. Here is a reference you can give him:
Hi Mike,
Did you wash the bottles and rinse them and then pump the water through the
instrument before adding reagents and purging all again? We had a problem
like this about 2 weeks ago. I think someone loaded the wrong bulk reagent
onto the instrument. Cleaning it, purging with water and
Its true, that test is too easy if you ask me. Dako handbook is all you'll
need.
Mark
On Thu, Feb 10, 2011 at 5:46 AM, Houston, Ronald
ronald.hous...@nationwidechildrens.org wrote:
Read and digest the Dako book and you will sail through the exam. For a
specialist type qualification it
Hi Milton,
We use Biocare's Universal Negative Control Serum in place of the antibodies
in the negative control staining protocol. We use normal prostate tissue to
show lack of p504s staining (to make sure it isn't overstaining normal
glands). The prostate cancer in the tissue control shows
So cruel!
On Fri, Feb 25, 2011 at 8:13 AM, sgoe...@mirnarx.com wrote:
It's sunshine and 75 here in Texas...nanny nanny...I do love my state's
weather!! We had shorts on yesterday =)
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin,
We do use the Ventana antibody for gastric cases. There is some difference
in reading the slide for the pathologist. I'll send you an article on it in
a seperate e-mail (so I can attach it).
Mark
On Fri, Mar 11, 2011 at 8:54 AM, Coppin, Margaret copp...@aruplab.comwrote:
Hello,
I am
Hi Jim,
If it's a new clone then a new valiation should be performed. I know of
many instances where one clone stains something that another doesn't.
If this is regarding Ventana not selling certains clones, those clones are
still available from Cell Marque directly.
Mark
On Mon, Mar 14, 2011
Well I wouldn't try and use a Ph.D. in religous studies to qualify for high
complexity testing...
On Wed, Mar 16, 2011 at 4:36 PM, Mark Turner mtur...@csilaboratories.comwrote:
Regarding CAP checklist, question ANP.23041. The operation of the imaging
system is performed by high-complexity
If anyone has the catalog numbers for the probes, that would help. I
couldn't get this info from Ventana when I asked. They said we have to ask
another lab, even for catalog numbers!
Mark
On Tue, Apr 19, 2011 at 7:59 AM, Sheila Fonner sfon...@labpath.com wrote:
SAME HERE!!! I would love
Hi Dana,
Invitrogen sells a rabbit polyclonal anti-pax-2 antibody that works well on
FFPE tissues (Cat # 180483). We use a multi-tissue control block for a
control. It has normal kidney to show the endothelial cells around
glomeruli and renal tubules staining, clear cell renal cell Ca to show
Hi Marcia,
Our slides for the Her2 CAP survey stained just fine. We're using a Ventana
XT to stain for Her2 by IHC.
Mark
On Wed, May 4, 2011 at 9:54 AM, Marcia Funk fu...@mercyhealth.com wrote:
CAP validation Her/2 slides not staining as clear as before. Is anyone
else having any issues ?
protocol. Then again, even deparaffinzing the slide using xylene
is off label, since it was approved using Hemo-D substitute. I imagine it
would be harder to go off label with Ventana's software. I guess you've
got to weigh the pros and cons as with everything.
Mark Tarango
On Sun, Jul 3, 2011 at 2
The only problem here is that the Cell Marque antibody is currently on
backorder until at least the end of August from what I've heard.
Mark
On Thu, Jul 7, 2011 at 12:36 PM, Angela Bitting akbitt...@geisinger.eduwrote:
CC1 standard, 32 min incubation with heat disabled. I use Cellmarques
We run the H. pylori IHC stain on all stomach specimens to improve turn
around time; however, when an inflammatory background is not appreciated by
the pathologist the charge is removed before sign-out and we eat the cost of
producing the slide.
Mark
On Wed, Jul 13, 2011 at 11:37 AM, Richard
My lab uses the SPT24 clone for TTF-1. Let me know if you'd like any
protocol info.
Mark
On Thu, Jul 14, 2011 at 11:47 AM, Inman, Anna anna.in...@stmarygj.orgwrote:
Is anyone using the SPT24 clone for TTF-1 using the Ultraview platform
on the Benchmark XT?
Our pathologists have read this
Hi Toni,
We put the control on the same slide and after it's stained and coverslipped
we draw a line to seperate the control and patient tissue with each side of
the line having either a C or P written on it.
Mark
On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni
Hi Anita,
We ran 40 cases and sent the same blocks to Phenopath for them to
run for comparision. There is a requistion for doing an ER/PR validation on
their website.
Mark
On Tue, Jul 19, 2011 at 1:43 PM, anita dudley azdud...@hotmail.com wrote:
I know this has been talked about before here
We do put controls on each slide in a case. Sometimes it's just one slide
that failed in a run. A batch control wouldn't tell you which slide failed
if there are no internal controls in the patient tissue. I personally
wouldn't feel comfortable doing IHC with batch controls.
Mark
On Wed, Jul
That's funny that you got cited for that. I was surprised to learn what our
safety officer setup at my lab for paraffin disposal. A commercial company
takes our paraffin and makes a product with it that is mixed with cedar
sawdust and paraffin wax for starting fires in the BBQ, fireplace, or
Hi Linda,
I had simliar trouble before I bought and added the extra HybReady to the
protocol (there is one that comes with the detection kit, but you need two
for good staining). It will clean up your stain and A LOT and remove those
blue splotches that show up when you don't use it.
Mark
On
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